The changes in growth status, ion contents, betaine levels and betaine\|aldehyde dehydrogenase (BADH) activity in seedlings of nonhalophyte barley treated with different concentrations of NaCl and KCl were studied. Re...The changes in growth status, ion contents, betaine levels and betaine\|aldehyde dehydrogenase (BADH) activity in seedlings of nonhalophyte barley treated with different concentrations of NaCl and KCl were studied. Results showed that the inhibition of growth in barley seedlings increased as enhancing NaCl and KCl for 96h and NaCl inhibitory degree was higher than that of KCl. The K\++ content of barley seedlings in NaCl was lower than the control, while the Na\++ content was higher, the levels of Na\++ fell in the seedlings treated with KCl, but K\++ levels rose, and K\++ content was higher than that of Na\++. The betaine levels of barley shoots rose with the increase in both external salt concentration and treatment time. Higher BADH activity was observed in low\|salt concentration but lower slightly in high\|salt concentration.展开更多
High active betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) is found in wild Suaeda liaotungensis . The enzyme is purified 206 fold with recovery of 1.5%. It have a specific activity of 2363 nmol/min·mg protein...High active betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) is found in wild Suaeda liaotungensis . The enzyme is purified 206 fold with recovery of 1.5%. It have a specific activity of 2363 nmol/min·mg protein and the molecular mass of each subunit is 64.5 kDa as determined by SDS PAGE.展开更多
Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） act...Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） activity and Glybet accumulation in the seedlings of salt-tolerant and salt-sensitive rice varieties grown under saline and acidic conditions peaked after treatment for 72 h and 96 h, respectively, and were higher than those grown under neutral pH and alkaline salt stress. A positive correlation was found between BADH activity and Glybet content in both salt-tolerant （P=0.71） and salt-sensitive （P=0.86） genotypes. The chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids contents in the stressed seedlings significantly decreased under both acidic and alkaline stresses, especially in the salt-sensitive genotype. Similarly, the maximum quantum yield of PSII （Fv/Fm）, photon yield of PSII （ФPSII）, non-photochemical quenching （NPQ） and net photosynthetic rate （Pn） in the stressed seedlings were inhibited, leading to overall growth reduction. The positive correlations between chlorophyll a content and Fv/Fm, total chlorophyll content and ФPSII, and Pn as well as Pn and leaf area in both salt-tolerant and salt-sensitive genotypes were found. Saline acidic and saline alkaline soils may play a key role affecting vegetative growth prior to the reproductive stage in rice plants.展开更多
In the previous report, we showed that the betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8 )from spinach leaves(Spinacia oleracea L.) was partially purified. It was observed that the activity of BADH fractionated by ...In the previous report, we showed that the betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8 )from spinach leaves(Spinacia oleracea L.) was partially purified. It was observed that the activity of BADH fractionated by saturation of 60％ ammonium sulfate展开更多
A genomic library derived from leaves of spinach was constructed with the λGem11_BamHI Arms as the vector. The library was screened using the BADH cDNA of mountain spinach as a probe and six positive clones were obta...A genomic library derived from leaves of spinach was constructed with the λGem11_BamHI Arms as the vector. The library was screened using the BADH cDNA of mountain spinach as a probe and six positive clones were obtained through three rounds of screening. One of the positive clones named D, which was hybridized with the 5′600 bp fragment of mountain spinach BADH cDNA, was selected and further analyzed. The size of the insert in clone D was about 12 kb. 8 856 nucleotides of the insert were sequenced which contained 2 459 nucleotides of 5′ noncoding region, 6 111 nucleotides of the complete sequence of the BADH gene, and 286 nucleotides of a 3′ noncoding region. The result of sequence analysis indicated that the BADH gene contained 14 introns and the junction sequences at splicing sites followed the GT_AG rule basically.展开更多
文摘The changes in growth status, ion contents, betaine levels and betaine\|aldehyde dehydrogenase (BADH) activity in seedlings of nonhalophyte barley treated with different concentrations of NaCl and KCl were studied. Results showed that the inhibition of growth in barley seedlings increased as enhancing NaCl and KCl for 96h and NaCl inhibitory degree was higher than that of KCl. The K\++ content of barley seedlings in NaCl was lower than the control, while the Na\++ content was higher, the levels of Na\++ fell in the seedlings treated with KCl, but K\++ levels rose, and K\++ content was higher than that of Na\++. The betaine levels of barley shoots rose with the increase in both external salt concentration and treatment time. Higher BADH activity was observed in low\|salt concentration but lower slightly in high\|salt concentration.
文摘High active betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) is found in wild Suaeda liaotungensis . The enzyme is purified 206 fold with recovery of 1.5%. It have a specific activity of 2363 nmol/min·mg protein and the molecular mass of each subunit is 64.5 kDa as determined by SDS PAGE.
基金supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC) (Grant No. BT-B-06-RG-14-4502)partly funded by the International Atomic Energy Agency (IAEA) (Contract No. 12998/R0)
文摘Glycinebetaine （Glybet） accumulation, photosynthetic efficiency and growth performance in indica rice cultivated under salt stress and extreme pH stress were investigated. Betaine aldehyde dehydrogenase （BADH） activity and Glybet accumulation in the seedlings of salt-tolerant and salt-sensitive rice varieties grown under saline and acidic conditions peaked after treatment for 72 h and 96 h, respectively, and were higher than those grown under neutral pH and alkaline salt stress. A positive correlation was found between BADH activity and Glybet content in both salt-tolerant （P=0.71） and salt-sensitive （P=0.86） genotypes. The chlorophyll a, chlorophyll b, total chlorophyll and total carotenoids contents in the stressed seedlings significantly decreased under both acidic and alkaline stresses, especially in the salt-sensitive genotype. Similarly, the maximum quantum yield of PSII （Fv/Fm）, photon yield of PSII （ФPSII）, non-photochemical quenching （NPQ） and net photosynthetic rate （Pn） in the stressed seedlings were inhibited, leading to overall growth reduction. The positive correlations between chlorophyll a content and Fv/Fm, total chlorophyll content and ФPSII, and Pn as well as Pn and leaf area in both salt-tolerant and salt-sensitive genotypes were found. Saline acidic and saline alkaline soils may play a key role affecting vegetative growth prior to the reproductive stage in rice plants.
基金Project supported by the National Natural Science Foundation of China and the National Science and Technology Key Research Plan.
文摘In the previous report, we showed that the betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8 )from spinach leaves(Spinacia oleracea L.) was partially purified. It was observed that the activity of BADH fractionated by saturation of 60％ ammonium sulfate
文摘A genomic library derived from leaves of spinach was constructed with the λGem11_BamHI Arms as the vector. The library was screened using the BADH cDNA of mountain spinach as a probe and six positive clones were obtained through three rounds of screening. One of the positive clones named D, which was hybridized with the 5′600 bp fragment of mountain spinach BADH cDNA, was selected and further analyzed. The size of the insert in clone D was about 12 kb. 8 856 nucleotides of the insert were sequenced which contained 2 459 nucleotides of 5′ noncoding region, 6 111 nucleotides of the complete sequence of the BADH gene, and 286 nucleotides of a 3′ noncoding region. The result of sequence analysis indicated that the BADH gene contained 14 introns and the junction sequences at splicing sites followed the GT_AG rule basically.
