干旱胁迫下白桦(Betula platyphylla)实生苗叶片的水分代谢与部分渗透调节物质的变化

在土壤干旱胁迫进程中 ,白桦实生苗叶片的自然含水量呈下降趋势 ;相对含水量总的变化趋势与自然含水量相同 ;束缚水含量及束缚水 /自由水比值显著升高 ,复水后 ,三者又重新恢复至正常水平。随着土壤水分逐渐减少 ,可溶性蛋白质的含量呈现减少趋势 ,复水后 ,可溶性蛋白质含量又可恢复至正常水平。白桦实生苗叶片可溶性糖含量增加 。

Analysis of three types of triterpenoids in tetraploid white birches(Betula platyphylla Suk.) and selection of plus trees

Betulin, oleanolic acid, and betulinic acid are naturally occurring pentacyclic triterpenoids that have significant medicinal value. Considerable amounts of these triterpenoids are available in the outer bark of white birch. In this study, we used ultrasound-assisted extraction （UAE） to extract triterpenoids from birch bark rapidly and with high efficiency. Using high performance liquid chro- matography （HPLC）, three types of triterpenoids were separated and detected. We examined the differences among triterpenoids extracted from diploid versus tetra- ploid white birch. Then, we used factor analysis to screen out tetraploid white birches with comprehensively excel- lent performance. The results indicate that the optimum conditions for extraction include the use of ethanol as an extraction solvent, a solid-to-liquid ratio of 0.1 g/10 ml, ultrasonic power set at 100 W, a temperature of 60 ℃ and an extraction time of 15 min. A reversed-phase C18 col- umn （4.6 mm × 250 mm × 5 μm） with a column tem- perature of 30 ℃ and the mobile phase composed of A （acetonitrile） and B （0.1% aqueous phosphoric acid, v/v） at a flow rate of 0.5 ml/min were used, and the detection wavelength was 195 nm. No significant difference wasobserved between diploid and tetraploid white birch in terms of the content of three types of triterpenoids （at a confidence level of 0.05）. As triterpenoid content, height, and DBH （diameter at breast height） are strongly interre- lated, we used factor analysis to evaluate all individuals, and we screened out six plus trees with excellent com- prehensive characters.

Construction and analysis of a subtracted cDNA library of Betula platyphylla female inflorescence

Female inflorescence of Betula platyphylla was sampled at an interval of eachtwo days to analyze the background of gene expression in floral phase. On the basis of SMARTstrategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA wasfrom that of the others by RT-PCR which were subsequently used to construct a subtracted cDNAlibrary. The result of the ESTs (expression sequence tags) blastX showed that the genes in thesubtracted cDNA library could be mainly clustered into 5 groups related to metabolism,transportation and signal transduction, cell cycle, stress response, and regulation. Therelationship between gene expression and development was also discussed.

Progeny test of tetraploid Betula platyphylla and preliminary selection of hybrid parents

Estimating genetic parameters of parental lines through progeny testing and choosing good hybrid parents are important for genetically improving seed orchard trees.In this study, 24 tetraploid progeny seedlings were used as experimental materials, which came from test cross design：six tetraploid Betula platyphylla lines（Q33, Q13, Q103,Q19, Q83 and Q14） as female parents and four individual B. platyphylla diploid lines（F3, F4, F9 and F11） as male parents were crossed. Variance analysis of height, diameter, height-to-diameter ratio, and internodal distance showed that the differences between hybrid combinations reached highly significant levels. Using multi-objective decisionmaking, we performed a comprehensive assessment of the various hybrid combinations. Using a selection rate of 20 % of the standard, five hybrids were selected;their genetic gains in average height, diameter, height-todiameter ratio, and internodal distance were 20.95, 6.07,13.07 and 8.96 %, respectively. We also analyzed the combining ability and genetic parameter effect values of parents and hybrid combinations. The combined analysis revealed that Q13, Q103, Q33 and Q83 were superior females; F3, F4, and F9 were superior males; and F3 × Q13, F4 × Q83 and F9 × Q33 were superior hybrid combinations. The heights and diameters of these progenies were 22.49 and 11.48 % greater than average,respectively.

A New Caffeoylquiniclactone, Neochlorgeniclatone from the Leaves of Betula platyphylla Suk.

A new caffeoylquiniclactone, named neochlorgeniclatone, was isolated from the leaves of Betula platyphylla Suk. The Structure was established by spectroscopic data.

A New Monoterpene Glucoside from the Leaves of Betula platyphylla Suk.

A new monoterpene glucoside. (2E,67.)-2.6-dimethyl-8-beta -D-glucosyloxy-2,6-octadienoic acid, was isolated from the leaves of Betula platyphylla Suk. The structure was established by spectroscopic data.

BpMADS12 gene role in lignin biosynthesis of Betula platyphylla Suk by transcriptome analysis

MADS-box transcription factors show highly diverse regulatory functions in a wide variety of organisms. In this study, we characterized a MADS-box gene （BpMADS12） from the white birch （Betula platyphylla Suk）. This gene is a member of the suppressor of overexpression of CO 1/tomato MADS 3 class of MADS-box genes. We generated lines overexpressing BpMADS12 and found that these had higher levels of lignin compared to that observed in nontransgenic lines. Transcriptome anal- ysis revealed numerous changes in gene expression patterns. In total, 8794 differentially expressed genes were identified, including 5006 upregulated unigenes and 3788 downregulated unigenes in BpMADS-overexpression lines. Differentially expressed genes involved in the pathways for lignin and brassinosteroid biosynthesis were significantly enriched and may have contributed to phenotypic changes. The results from a quantitative RT-PCR analysis were consistent those obtained with the transcriptome analysis.Our transcriptome analysis, in combination with measure- ment of lignin level, indicated that BpMADS12 promotes lignin synthesis through regulation of key enzymes in response to brassinosteroid signaling. These results suggest that this MADS-box protein is crucial to all subsequent structural events and provide a good foundation for studies aiming to elucidate the developmental mechanisms underlying formation of wood.

Selection of elite lines of BpGH3.5-transgenic Betula platyphylla using growth adaptability analysis

White birch(Betula platyphylla)is precious material for pulpwood and widely distributed in 14 provinces of China.Previous study indicated that inhibited expression of a gene encoding an auxin amide synthase,BpGH3.5,in transgenic plants reduced the level of IAA–amino acid conjugation,resulting in more free IAA,thereby better growth of birch.Utilizing transgenic-B pGH3.5 lines to increase wood production in a wide range of environments is the goal for breeders.In three field trials here,we measured tree height,diameter at breast height,and volume of 16 BpGH3.5-transgenic 7-year-old white birch lines(including 12 antisense strand lines and 4 overexpression lines)and a wild-type white birch line from three sites that varied greatly in their environmental conditions.To select elite BpGH3.5-transgenic lines for each target environment,we used an additive main effects and multiplicative interaction model to analyze genotype by environment interaction,growth adaptability and stability.The selection criteria for elite transgenic lines were set as the average volume plus 0.75 times the standard deviation for the tested lines at each test site.Results showed that the effect of line and site for height was highly significant(P<0.01),and the effect of line×site was significant(P<0.05);selected as the elite lines were FG12,FG13 and FG27 at the Maoershan Experimental Forest Farm,FG13 and FG32 at the Shidaohe Forest Farm,and FG3 and FG31 at the Ecological Experiment Forest Farm.These seven high-yield,stable lines can now be tested in production trials or adjacent trial areas with similar environmental conditions,while the high-yield,unstable lines should be tested in production trials in areas deemed suitable for their growth.These results provide guidance on which released transgenic elite lines will grow best in a wide range of conditions.

G×E Analysis of Growth Traits of Betula platyphylla Clones at Three Separated Sites in Northeastern China

To select elite materials,the growth traits of 32 Betula platyphylla clones at three separated northern sites in Northeast China were investigated and analyzed.The results showed that there were significant differences among all variation sources in the different investigated traits(P<0.01).Except for the carbon contents,all the coeffi-cients of phenotypic variation of the other investigated traits were higher than 10%.The repeatability of different traits ranged from 0.760 to 0.998.Correlation analysis showed that tree height were significantly correlated with diameter at breast height,but neither was significantly correlated with leaf traits nor element contents.Additive main effects and multiplicative interaction analysis showed that genotype,environment and genotype×environment interactions were significantly different in diameter at breast height,which indicated that environment had a significant effect on genotype.Comprehensive assessment results showed that three clones with high and stable diameters at breast height were selected,and the genetic gains of diameter at breast height on sites Maoershan,Qingan,and Yongji were 21.24%,20.58%,and 38.65%,respectively.The results could provide a theoretical basis for elite clone selection in B.platyphylla and other broad leaved species.

Genetic Effect Analysis of Quantitative Traits and Selection of Excellent Population in Betula platyphylla var.mandshurica Hara.

Betula platyphylla var. mandshurica Hara. is a geographical variant of B. platyphylla Suk. with strong adaptability, rapid growth, fine texture, wide application and other notable features. In order to investigate the genetic effects of quantitative traits and specificity of different populations of B. platyphylla var. mandshurica Hara. , by permanent sample plot continuous measurement and temporary sample plot random investigation, B. platyphyUa var. mandshurica Hara. populations naturally distributed in Zhangguangcailing Mountain and Laoye Mountain in Changbai Mountain Range were used as experimental materials to systemati- cally analyze the growth dynamic patterns of plant height and diameter at breast height, and comprehensively evaluate the genetic effects of various quantitative traits. According to the results, site conditions suitable for B. platyphylla var. mandshurica Hara. were classified into four grades according to the absolute value of partial correlation coefficients. The growth process of B. platyphylla var. mandshurica Hara. stand generally underwent rapid growth period, steady growth period and slow growth period. The growth of B. platyphylla var. mandshurica Hara. stand at different growth stages varied signitlcantly among various site grades, but the growth trend was basieally the same. Plant height, diameter at breast height, east-west crown diameter and north-south crown diameter varied significantly among B. platyphylla vat. mandshurica Hara. populations, exhibiting extremely significant differences. The heritability of four growth traits was 0. 558,0. 762, 0. 652 and 0. 722, respectively; the genetic gain was 8.09%, 17.67%, 14.56% and 17.53%, respectively. Based on comprehensive evaluation of various indicators, MES （Maoershan） was identified as an excellent population, which exhibited minor variations between individuals within the population, stable growth, strong growth potential and remarkable genetie effects.

Genetic Improvement of Betula platyphylla Suk.in China:A Review

Birch(Betula platyphylla Suk.),distributed in Eurasia,North America,and Australia,is a kind of cold-resistant,fast-growing,and vital pulpwood tree species.It is also one of the most important ecological restoration tree species with high values of economic benefits in Northeast China.To improve the genetic gain and expand the economic benefit of B.platyphylla,many genetic improvements have been carried out.In China,B.platyphylla is widely distributed and varied,and there are many varieties with excellent genetic characteristics.In this paper,the genetic improvement of B.platyphylla was reviewed,and the previous research results were discussed from two aspects:conventional breeding and molecular breeding.Some problems and corresponding solutions in the genetic improvement were put forward to provide ideas for B.platyphylla breeding in the future.

Growth Variation and Early Selection of Betula Platyphylla in Natural Stands

The stem analysis of Betula platyphylla in natural forest at the Maoershan Forest Experimental Station of Northeast Forestry Universitu. showed that the growth variation of the trees. including height and diameter breast height decreaseswith the increase of the age. There is the turning point for the growth variation of the tree from acute change to relative sta-bility when the trees are in eighteen years old. There are signiticant difttrences among the height. diameter and volumegrowth of the trees at that time. Theretbre. the optimal age for carly seleetion of this species in natural stands is eighteenyears old (18 a). Diameter is used as main index for eary seleclion and height growth as auxiliary one.

Plant hormone treatments to alleviate the effects of salt stress on germination of Betula platyphylla seeds

We analyzed the germination traits (germination rate, germination potential, and germination index) of seeds from 23 half-sib families of birch (Betula platyphylla). The germination rates of the 23 families ranged from 38.57 to 76.50%. Seeds from the eight families germinating at the highest rates were then used for germination experiments under salt stress. Germination rate, germination potential, and germination index of these eight half-sib families decreased with increasing salt concentrations. The effects of four different hormones (6-benzylaminopurine, 6BA;gibberellin, GA3;naphthalene acetic acid, NAA;and melatonin) at various concentrations on the germination of seeds from the eight half-sib birch families under salt stress were investigated. Treatments with 6BA and GA3 alleviated the effects of salt stress on seed germination, whereas treatments with NAA and melatonin aggravated the effects of salt stress. The most effective treatments for alleviating the effects of salt stress on birch seed germination were 10 mg/L 6BA and 300 mg/L GA3. This study provides practical information for screening birch families for salt tolerance at the seed germination stage, and for cultivation of birch in saline environments.

Expression profiles of genes regulated by BplMYB46 in Betula platyphylla

The transcription factor BplMYB46 has been identified as a regulator of abiotic stress responses and promoter of secondary wall deposition in Betula platyphylla.To investigate the downstream targets of BplMYB46,the expression profiles of genes in stems from BplMYB46-overexpressing(OE)and BplMYB46-silencing(SE)plants were studied.In OE stems,952 genes were upregulated,and 1469 were downregulated in comparison to SE stems.In a KEGG pathway enrichment analysis of differentially expressed genes(DEGs),1387 differentially expressed genes were annotated for 117 metabolic pathways.DEGs were abundant for metabolic pathway,secondary metabolite biosynthesis,plant hormone signal transduction,phenylpropanoid and flavonoid biosynthesis.DEGs were implicated lignin or cellulose biosynthesis,cell wall modification,xylem development,disease resistance,stress responses,and anthocyanin biosynthesis.These results suggested that BplMYB46 regulates cell wall development and stress resistance by affecting the expression of these genes.Our study further elucidates the mechanism by which BplMYB46 mediates abiotic stress responses and secondary cell wall biosynthesis in birch.

Expression profiling of the BpIAA gene family and the determination of IAA levels in Betula platyphylla tetraploids

Betula platyphylla Sukaczev tetraploids have significantly larger leaf, fruit and stoma (gigantic phenotype) than diploids of the same species;however, the mechanism underlying this difference remains unclear. Tetraploid B. platyphylla transcriptome data have indicated that the expression of genes related to indole-3-acetic acid (IAA) biosynthesis and signal transduction was altered after genome duplication. IAA exerts pleiotropic effects on growth and development by inducing the expression of Aux/IAA. We identified 20 Aux/IAA genes (BpIAA1– BpIAA20) in B. platyphylla distributed across 10 chromosomes. Multiple alignment and motif analyses revealed that nine BpIAA proteins shared all four conserved domains. Phylogenetic analysis indicated that Aux/IAA families were divided into four subfamilies and that there were two pairs of BpIAA sister genes. The BpIAAs were differentially expressed in diploids and tetraploids. Moreover, the expression levels of the nine BpIAA genes were specifically up-regulated in tetraploids from June to September compared with May (except August 5th) in tetraploids, while they were down-regulated in diploids. IAA levels were more than twofold higher in tetraploids than diploids during the vegetative season. These results indicate that genome duplication of B. platyphylla caused the up-regulated of genes involved in IAA synthesis, and the increased concentration of IAA may induce the constitutive expression of 20 BpIAA genes. Therefore, the significant changes in the expression patterns of the BpIAAs contributed to the gigantic phenotype of tetraploids to some extent. Our research sheds light on the phenotypic variations observed in B. platyphylla tetraploids.

A phytosociological study on Betula Platyphylla forestsin Daxing'an Mountains of China

For the first time in this paper the forests of Betula platyphylla in Daxing’an Mountains are Subdivided by phytosociological methods. Three community types of B. platyphylla forest have been differentiated, e.g., Rhododendron dahuricum-Betula platyphylla Corylus, Corylus heterophylla-Betula platyphylla community and Arternisia stolonifera-Betula platyphylla community. The distributed elevation, stand height, differential species, major composition species of tree layer, shrub layer and herb layer for each community were detail

Comparison, Selection and Progeny Test of Natural Populations of White Birch (Betula platyphylla Suk.) in Northern China

[Objectives]This study was conducted to rationally and effectively use natural genetic resources of white birch(Betula platyphylla Suk.),and improve the scientific and technological content of improved varieties.[Methods]With the progeny of the white birch populations naturally distributed in northern China as objects,based on genetic effect analysis and comprehensive evaluation,the genetic variation,heritability and genetic gain of the growth characters in the progeny forest were systematically analyzed,by which populations or individuals with good growth vigor and strong stress resistance and adaptability were finally selected.[Results]There were extremely significant differences in the growth characters between the natural populations of white birch in northern China.The variation was the smallest in tree height,followed by crown width,and largest in DBH,and the average coefficients of variation were 20.56%,32.25%and 33.00%,respectively.The differences in characters between progeny of the same population were also extremely significant.The growth characters of white birch were more controlled by genes,and the genetic gains were larger.The heritability of tree height,DBH and crown width was 0.971,0.816 and 0.576,respectively,and the genetic gains were 15.97%,21.54%and 13.87%,respectively.[Conclusions]With the growth characters as the main evaluation indexes,taking various influencing factors into account,the Shanxi[SX]population,Hebei[HB]population and Liaoning[LN]population were selected to be excellent populations,and LN305,SX516 and HB403 were excellent individuals,which are advised to be widely used in multi-generation genetic improvement breeding.

Effect of MeJA and Light on the Accumulation of Betulin and Oleanolic Acid in the Saplings of White Birch (<i>Betula platyphylla</i>Suk.)

In this study, we investigated the effect of different types of light and MeJA treatment on the accumulation of betulin and oleanolic acid in various organs of white birch. Our results showed that betulin and oleanolic were accumulated mainly in the stalk skin. The content of both substances in the stalk skin was significantly affected by seasons with a peak accumulation in August. The content of oleanolic and betulin was significantly decreased in the stem skin treated with 4 types of light (red, yellow, blue and green) compared with the plant with normal illumination. In contrast, oleanolic acid in leaves was increased by 13.28 folds when the white birch was treated with green light. Betulin was increased by 1.959 folds in leaves of white birch treated with blue light. The highest content of betulin and oleanolic acid in various organs of birch with appropriate shading treatment (light transmittance: 50%) was increased by 45.09% and 30.50%, respectively, in comparison with those with non-shading treatment. Content of oleanolic acid and betulin can be significantly improved in various parts of birch after treatment with different concentration of MeJA. The study lays the foundation to metabolic regulation of oleanolic acid and betulin in birch.

Differences and similarities in radial growth of Betula species to climate change

Betula platyphylla and Betula costata are important species in mixed broadleaved-Korean pine(Pinus koraiensis)forests.However,the specific ways in which their growth is affected by warm temperatures and drought remain unclear.To address this issue,60 and 62 tree-ring cores of B.platyphylla and B.costata were collected in Yichun,China.Using dendrochronological methods,the response and adaptation of these species to climate change were examined.A“hysteresis effect”was found in the rings of both species,linked to May–September moisture conditions of the previous year.Radial growth of B.costata was positively correlated with the standardized precipitation-evapotranspiration index(SPEI),the precipitation from September to October of the previous year,and the relative humidity in October of the previous year.Growth of B.costata is primarily restricted by moisture conditions from September to October.In contrast,B.platyphylla growth is mainly limited by minimum temperatures in May–June of both the previous and current years.After droughts,B.platyphylla had a faster recovery rate compared to B.costata.In the context of rising temperatures since 1980,the correlation between B.platyphylla growth and monthly SPEI became positive and strengthened over time,while the growth of B.costata showed no conspicuous change.Our findings suggest that the growth of B.platyphylla is already affected by warming temperatures,whereas B.costata may become limited if warming continues or intensifies.Climate change could disrupt the succession of these species,possibly accelerating the succession of pioneer species.The results of this research are of great significance for understanding how the growth changes of birch species under warming and drying conditions,and contribute to understanding the structural adaptation of mixed broadleaved-Korean pine(Pinus koraiensis)forests under climate change.

Diacylglycerol Compounds from Barks of Betula platyphylla with Inhibitory Activity against Acyltransferase

Objective To identify the active compounds from the barks of Betula platyphylla for inhibitory on diacylglycerol acyltransferase（DGAT1）.Methods Bioassay-guided fractionation resulted in the isolation of DGAT1 inhibitory activity of lupane triterpenes.Results Ten compounds were identified as lupenone（1）,lupeol（2）,betulinic acid（3）,betulinaldehyde（4）,betulin（5）,3-deoxybetulonic acid（6）,glochidonol（7）,lup-20/29-ene-1β/3β-diol（8）,3α-hydroxy-lup-20（29）-en-23,28-dioic acid（9）,and 3α,11α-dihydroxy-23-oxo-lup-20（29）-en-28-oic acid（10）.Compounds 3-6,9,and 10 inhibited DGAT1 with IC50 values ranging from（11.2±0.3）to（38.6±1.2）μmol/L.Conclusion Compounds 6,9,and 10 are first isolated from the barks of B.platyphylla.,and compounds 3-6,9,and 10 from the barks of B.platyphylla are responsible for the inhibition on DGAT1.