A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon ma...A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon master with positive features.The PDMS replica was fabricated by casting PDMS prepolymer against the silicon master,where an optical fiber was first fixed on the end of separation microchannel.To improve the rigid characteristics of integrated PDMS microchip,the chips were subsequently assembled by reversible sealing against glass plate.A blue light emitting diode (LED) was used as excitation light sources for inducing fluorescence detection through coupling LED light into the optical fiber.As an application, integrated PDMS microchip was tested in the capillary electrophoresis separation of DNA markers.The results showed that DNA markers could be effectively separated and detected except for the segments of 271 and 281.展开更多
欧司朗光电半导体推出的RGB Multi Chip LED,被应用于FormoLight显示器上。其紧凑的外形设计,使得它们在不同大小体积的LED视频显示器上可以显示特殊的图像格式。该显示器区别于液晶显示器,LED不再作为人们看不到的背光光源，在这种应...欧司朗光电半导体推出的RGB Multi Chip LED,被应用于FormoLight显示器上。其紧凑的外形设计,使得它们在不同大小体积的LED视频显示器上可以显示特殊的图像格式。该显示器区别于液晶显示器,LED不再作为人们看不到的背光光源，在这种应用上，LED在显示器的表面清晰可见。展开更多
基金This present work was supported by grant from the National Natural Science Foundation of China (N0．60501020， No．60341005 and No．20299030)
文摘A PDMS electrophoresis microchip,which integrated with optical fiber for fluorescence detection,was fabricated by using silicon master.A deep reactive ion etches (DRIE) technology was used to fabricate the silicon master with positive features.The PDMS replica was fabricated by casting PDMS prepolymer against the silicon master,where an optical fiber was first fixed on the end of separation microchannel.To improve the rigid characteristics of integrated PDMS microchip,the chips were subsequently assembled by reversible sealing against glass plate.A blue light emitting diode (LED) was used as excitation light sources for inducing fluorescence detection through coupling LED light into the optical fiber.As an application, integrated PDMS microchip was tested in the capillary electrophoresis separation of DNA markers.The results showed that DNA markers could be effectively separated and detected except for the segments of 271 and 281.
