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Application of CFD and FEA Coupling to Predict Structural Dynamic Responses of A Trimaran in Uni-and Bi-Directional Waves
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作者 LIAO Xi-yu XIA Jin-song +4 位作者 CHEN Zhan-yang TANG Qin ZHAO Nan ZHAO Wei-dong GUI Hong-bin 《China Ocean Engineering》 SCIE EI CSCD 2024年第1期81-92,共12页
To predict the wave loads of a flexible trimaran in different wave fields,a one-way interaction numerical simulation method is proposed by integrating the fluid solver(Star-CCM+)and structural solver(Abaqus).Differing... To predict the wave loads of a flexible trimaran in different wave fields,a one-way interaction numerical simulation method is proposed by integrating the fluid solver(Star-CCM+)and structural solver(Abaqus).Differing from the existing coupled CFD-FEA method for monohull ships in head waves,the presented method equates the mass and stiffness of the whole ship to the hull shell so that any transverse and longitudinal section stress of the hull in oblique waves can be obtained.Firstly,verification study and sensitivity analysis are carried out by comparing the trimaran motions using different mesh sizes and time step schemes.Discussion on the wave elevation of uni-and bi-directional waves is also carried out.Then a comprehensive analysis on the structural responses of the trimaran in different uni-directional regular wave and bi-directional cross sea conditions is carried out,respectively.Finally,the differences in structural response characteristics of trimaran in different wave fields are studied.The results show that the present method can reduce the computational burden of the two-way fluid-structure interaction simulations. 展开更多
关键词 CFD-FEA fluid-structure coupling structural responses TRIMARAN bi-directional cross sea
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A chaotic hierarchical encryption/watermark embedding scheme for multi-medical images based on row-column confusion and closed-loop bi-directional diffusion
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作者 张哲祎 牟俊 +1 位作者 Santo Banerjee 曹颖鸿 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第2期228-237,共10页
Security during remote transmission has been an important concern for researchers in recent years.In this paper,a hierarchical encryption multi-image encryption scheme for people with different security levels is desi... Security during remote transmission has been an important concern for researchers in recent years.In this paper,a hierarchical encryption multi-image encryption scheme for people with different security levels is designed,and a multiimage encryption(MIE)algorithm with row and column confusion and closed-loop bi-directional diffusion is adopted in the paper.While ensuring secure communication of medical image information,people with different security levels have different levels of decryption keys,and differentiated visual effects can be obtained by using the strong sensitivity of chaotic keys.The highest security level can obtain decrypted images without watermarks,and at the same time,patient information and copyright attribution can be verified by obtaining watermark images.The experimental results show that the scheme is sufficiently secure as an MIE scheme with visualized differences and the encryption and decryption efficiency is significantly improved compared to other works. 展开更多
关键词 chaotic hierarchical encryption multi-medical image encryption differentiated visual effects row-column confusion closed-loop bi-directional diffusion transform domain watermark embedding
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基于改进平方根无迹粒子滤波的RAM类目标跟踪
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作者 张涵 张逊 梁燊 《指挥控制与仿真》 2024年第5期55-61,共7页
RAM类目标运动复杂,对跟踪系统提出了更高的要求。针对粒子滤波在解决RAM类目标跟踪过程中存在的粒子退化等问题,通过无迹变换计算得出后验概率密度函数充当粒子滤波的先验概率密度函数,提高后验概率密度函数拟合程度,最终提高粒子的有... RAM类目标运动复杂,对跟踪系统提出了更高的要求。针对粒子滤波在解决RAM类目标跟踪过程中存在的粒子退化等问题,通过无迹变换计算得出后验概率密度函数充当粒子滤波的先验概率密度函数,提高后验概率密度函数拟合程度,最终提高粒子的有效性。同时,在无迹变换过程中采用qr分解取代部分Cholesky分解,使输出信号方差矩阵保持非负性。最后,针对一段RAM类目标轨迹建立动力学模型,通过Matlab进行仿真,验证了该算法的有效性。 展开更多
关键词 平方根无迹粒子滤波 无迹变换 ram类目标 粒子退化
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浸没燃烧式气化器SCV的RAM分析及可用度优化
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作者 周文安 王海清 +1 位作者 陈桂海 韩思杰 《高校化学工程学报》 EI CAS CSCD 北大核心 2024年第2期302-309,共8页
浸没燃烧式气化器(SCV)是液化天然气LNG应急调峰站的关键核心系统,系统中出现设备故障会导致天然气供应中断,将造成显著社会影响与生产损失。为了评估SCV系统的生产性能及提高运行稳定性,提出并建立SCV-RAM分析模型,应用蒙特卡洛模拟仿... 浸没燃烧式气化器(SCV)是液化天然气LNG应急调峰站的关键核心系统,系统中出现设备故障会导致天然气供应中断,将造成显著社会影响与生产损失。为了评估SCV系统的生产性能及提高运行稳定性,提出并建立SCV-RAM分析模型,应用蒙特卡洛模拟仿真定量辨识SCV的瓶颈单元设备并针对性设计改进方案。结果表明,SCV系统易发生故障的单元按贡献度依次分别是:助燃空气单元、循环冷却水单元,其中导致系统不可用度增大的具体关键设备为水泵、电机以及鼓风机。据此对关键设备的预防性维修间隔进行优化,SCV系统的全寿命周期统计可用度从90.17%提高到94.93%,总停机时间减少了3478h。分析结果有助于优化SCV系统的维修资源分配,为实现SCV系统长周期稳定运行提供技术支持。 展开更多
关键词 浸没燃烧式气化器(SCV) 可靠性、可用性和维护性分析(ram分析) 关键设备 可用度优化
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Text Sentiment Analysis Based on Multi-Layer Bi-Directional LSTM with a Trapezoidal Structure
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作者 Zhengfang He Cristina E.Dumdumaya Ivy Kim D.Machica 《Intelligent Automation & Soft Computing》 SCIE 2023年第7期639-654,共16页
Sentiment analysis,commonly called opinion mining or emotion artificial intelligence(AI),employs biometrics,computational linguistics,nat-ural language processing,and text analysis to systematically identify,extract,m... Sentiment analysis,commonly called opinion mining or emotion artificial intelligence(AI),employs biometrics,computational linguistics,nat-ural language processing,and text analysis to systematically identify,extract,measure,and investigate affective states and subjective data.Sentiment analy-sis algorithms include emotion lexicon,traditional machine learning,and deep learning.In the text sentiment analysis algorithm based on a neural network,multi-layer Bi-directional long short-term memory(LSTM)is widely used,but the parameter amount of this model is too huge.Hence,this paper proposes a Bi-directional LSTM with a trapezoidal structure model.The design of the trapezoidal structure is derived from classic neural networks,such as LeNet-5 and AlexNet.These classic models have trapezoidal-like structures,and these structures have achieved success in the field of deep learning.There are two benefits to using the Bi-directional LSTM with a trapezoidal structure.One is that compared with the single-layer configuration,using the of the multi-layer structure can better extract the high-dimensional features of the text.Another is that using the trapezoidal structure can reduce the model’s parameters.This paper introduces the Bi-directional LSTM with a trapezoidal structure model in detail and uses Stanford sentiment treebank 2(STS-2)for experiments.It can be seen from the experimental results that the trapezoidal structure model and the normal structure model have similar performances.However,the trapezoidal structure model parameters are 35.75%less than the normal structure model. 展开更多
关键词 Text sentiment bi-directional LSTM Trapezoidal structure
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A Modified Bi-Directional Evolutionary Structural Optimization Procedure with Variable Evolutionary Volume Ratio Applied to Multi-Objective Topology Optimization Problem
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作者 Xudong Jiang Jiaqi Ma Xiaoyan Teng 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第4期511-526,共16页
Natural frequency and dynamic stiffness under transient loading are two key performances for structural design related to automotive,aviation and construction industries.This article aims to tackle the multi-objective... Natural frequency and dynamic stiffness under transient loading are two key performances for structural design related to automotive,aviation and construction industries.This article aims to tackle the multi-objective topological optimization problem considering dynamic stiffness and natural frequency using modified version of bi-directional evolutionary structural optimization(BESO).The conventional BESO is provided with constant evolutionary volume ratio(EVR),whereas low EVR greatly retards the optimization process and high EVR improperly removes the efficient elements.To address the issue,the modified BESO with variable EVR is introduced.To compromise the natural frequency and the dynamic stiffness,a weighting scheme of sensitivity numbers is employed to form the Pareto solution space.Several numerical examples demonstrate that the optimal solutions obtained from the modified BESO method have good agreement with those from the classic BESO method.Most importantly,the dynamic removal strategy with the variable EVR sharply springs up the optimization process.Therefore,it is concluded that the modified BESO method with variable EVR can solve structural design problems using multi-objective optimization. 展开更多
关键词 bi-directional evolutionary structural optimization variable evolutionary volume ratio multi-objective optimization weighted sum topology optimization
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多腔梯度序贯充压联合Caprini RAM量表指导下的康复方案对肺癌患者术后深静脉血栓的影响
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作者 翟巨婵 范园园 潜艳 《临床护理杂志》 2024年第3期18-21,共4页
目的探讨多腔梯度序贯充压联合Caprini RAM量表指导下的康复方案对肺癌患者术后深静脉血栓的影响。方法选择2020年1月-2023年1月我院收治的肺癌患者150例,将2020年1月-2021年12月收治的75例患者列为常规组,2022年1月-2023年1月收治的75... 目的探讨多腔梯度序贯充压联合Caprini RAM量表指导下的康复方案对肺癌患者术后深静脉血栓的影响。方法选择2020年1月-2023年1月我院收治的肺癌患者150例,将2020年1月-2021年12月收治的75例患者列为常规组,2022年1月-2023年1月收治的75例患者列为观察组。常规组实施多腔梯度序贯充压治疗与传统护理,观察组实施多腔梯度序贯充压联合Caprini RAM量表指导下的康复方案。观察两组DVT预防知识、结局相关指标和护理满意度。结果观察组干预后DVT预防知识得分明显高于常规组,患者下肢DVT发生率、D-二聚体、下肢周径差均明显低于常规组,住院时间短于常规组(P<0.05),观察组护理满意度明显高于常规组(P<0.05)。结论多腔梯度序贯充压联合Caprini RAM量表指导下的康复方案可提高肺癌患者术后DVT预防知识水平,促进康复进程,提升患者满意度。 展开更多
关键词 肺肿瘤 Caprini ram量表 深静脉血栓形成 多腔梯度序贯充压
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Wave propagation responses of porous bi-directional functionally graded magneto-electro-elastic nanoshells via nonlocal strain gradient theory
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作者 Xinte WANG Juan LIU +2 位作者 Biao HU Bo ZHANG Huoming SHEN 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2023年第10期1821-1840,共20页
This study examines the wave propagation characteristics for a bi-directional functional grading of barium titanate(BaTiO_(3)) and cobalt ferrite(CoFe_(2)O_(4)) porous nanoshells,the porosity distribution of which is ... This study examines the wave propagation characteristics for a bi-directional functional grading of barium titanate(BaTiO_(3)) and cobalt ferrite(CoFe_(2)O_(4)) porous nanoshells,the porosity distribution of which is simulated by the honeycomb-shaped symmetrical and asymmetrical distribution functions.The nonlocal strain gradient theory(NSGT) and first-order shear deformation theory are used to determine the size effect and shear deformation,respectively.Nonlocal governing equations are derived for the nanoshells by Hamilton's principle.The resulting dimensionless differential equations are solved by means of an analytical solution of the combined exponential function after dimensionless treatment.Finally,extensive parametric surveys are conducted to investigate the influence of diverse parameters,such as dimensionless scale parameters,radiusto-thickness ratios,bi-directional functionally graded(FG) indices,porosity coefficients,and dimensionless electromagnetic potentials on the wave propagation characteristics.Based on the analysis results,the effect of the dimensionless scale parameters on the dispersion relationship is found to be related to the ratio of the scale parameters.The wave propagation characteristics of nanoshells in the presence of a magnetoelectric field depend on the bi-directional FG indices. 展开更多
关键词 bi-directional functionally graded(FG) wave propagation dimensionless magneto-electro-elastic(MEE)nanoshell nonlocal strain gradient theory(NSGT) porosity
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热蒙机型RAM发电机仿真建模与保护配置及定值整定研究
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作者 邹伟 李润培 +2 位作者 桂林 陈鑫 王祥珩 《水电与抽水蓄能》 2024年第5期26-32,42,共8页
大亚湾核电站棒控电源(rotating asynchronous machine,RAM)是两台带飞轮的电动发电机,采用的是热蒙机型(进口机型/有刷相复励励磁方式),由于其保护装置并非微机型且缺乏故障录波装置,难以判断其他核电站发生的RAM系统失磁与过流保护失... 大亚湾核电站棒控电源(rotating asynchronous machine,RAM)是两台带飞轮的电动发电机,采用的是热蒙机型(进口机型/有刷相复励励磁方式),由于其保护装置并非微机型且缺乏故障录波装置,难以判断其他核电站发生的RAM系统失磁与过流保护失配导致停堆事故是否存在共性风险。文章首先建立了热蒙机型RAM系统的PSCAD模型,通过真机试验结果和机端短路故障的仿真分析证明其正确性;然后通过不同负载工况下发生不同类型失磁故障后非故障机机端电流的变化情况,对热蒙机型RAM发电机过流保护的配置及定值整定进行合理化建议,避免RAM系统冗余设计失效;最后进一步对热蒙机型RAM系统出口断路器过流保护的配置及定值整定进行了优化。相关工作为热蒙机型RAM发电机保护装置国产化改造奠定了基础。 展开更多
关键词 棒控电源 电动发电机 失磁故障 机端短路 保护配置及定值整定
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Overall Bi-directional Panoramic Exploration of the Solar System 被引量:1
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作者 WU Zhimin ZHAO Jincai 《Aerospace China》 2019年第2期46-52,共7页
An overall bi-directional panoramic solar system exploration activity,not just looking at the solar system at a macro level and helping to build a simulation model for the solar system,but the probe will also be able ... An overall bi-directional panoramic solar system exploration activity,not just looking at the solar system at a macro level and helping to build a simulation model for the solar system,but the probe will also be able to explore the Milky Way and the vast universe from a much wider perspective.By observing the characteristics of the solar system,solar wind,ionization envelope and other parameters from a bi-directional panorama on both sides of the solar ecliptic plane,it will assist the scientific community and human kind to understand the solar system in a more extensive,deeper and systematic way than before.The exploration can be done in two steps.The first step is to launch a solar polar probe.Secondly to launch a bi-directional probe orbiting the galaxy in sync with the sun. 展开更多
关键词 overall bi-directional PANOramIC EXPLORATION SOLAR SYSTEM
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基于FPGA的异步双端口RAM芯片原型验证研究
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作者 巩京爽 靳旭 +2 位作者 武方达 林子明 刘光宇 《铁路通信信号工程技术》 2024年第9期21-25,38,共6页
为保证芯片功能符合预期,提高流片成功率,异步双端口RAM芯片设计在流片前进行充分的功能验证,其中FPGA原型验证能够重现芯片的实际工作环境,以此验证芯片设计的有效性。基于FPGA的异步双端口RAM芯片原型验证根据芯片定义,选择使用Intel... 为保证芯片功能符合预期,提高流片成功率,异步双端口RAM芯片设计在流片前进行充分的功能验证,其中FPGA原型验证能够重现芯片的实际工作环境,以此验证芯片设计的有效性。基于FPGA的异步双端口RAM芯片原型验证根据芯片定义,选择使用Intel公司的10M40DCF256FPGA为核心硬件搭建FPGA原型验证平台,在平台上通过完成从ASIC到FPGA的代码移植实现芯片原型,然后根据设计需求编写测试程序实现对该芯片的FPGA原型验证。测试结果均符合预期,表明异步双端口RAM芯片的功能符合设计需求,避免项目时间和资金成本的额外增加。 展开更多
关键词 异步双端口ram芯片 FPGA 原型验证
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浅析基于RAMS的轨道交通车辆状态评估方法
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作者 戴杰 廖玻 《中国设备工程》 2024年第7期272-273,共2页
随着轨道交通的快速发展,对其关键部件可靠性、可用性、安全性以及维修性的评估方法提出了更高的要求。因此提出了基于RAMS的轨道交通车辆状态评估方法,应用RAMS对关键部件进行状态评估,其中利用安全性确定部件维修方式,可靠性确定部件... 随着轨道交通的快速发展,对其关键部件可靠性、可用性、安全性以及维修性的评估方法提出了更高的要求。因此提出了基于RAMS的轨道交通车辆状态评估方法,应用RAMS对关键部件进行状态评估,其中利用安全性确定部件维修方式,可靠性确定部件维修时间间隔,可用性判断部件维修状态。 展开更多
关键词 车辆状态评估 ramS 故障分布函数 风险优先数
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基于双口RAM接收并处理1553B总线数据的方法研究
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作者 邓凯 蔡东博 赵昶宇 《科技与创新》 2024年第12期162-164,168,共4页
为了保证主机板和1553B板通过双口RAM(Random Access Memory,随机存取存储器)进行1553B总线通信时的正确性和可靠性,提出了一种利用双口RAM接收并处理1553B总线数据的方法,用于实现1553B的BC(Bus Controller,总线控制器)功能。该方法对... 为了保证主机板和1553B板通过双口RAM(Random Access Memory,随机存取存储器)进行1553B总线通信时的正确性和可靠性,提出了一种利用双口RAM接收并处理1553B总线数据的方法,用于实现1553B的BC(Bus Controller,总线控制器)功能。该方法对双口RAM中的地址按照子地址号进行地址分配,通过建立相同子地址序号的数据链表和建立环形缓冲区,完美解决了发送BC→RT(Remote Terminal,远程终端)报文时相同子地址数据覆盖和主机端发送的子地址序号顺序与1553B板端发送的子地址序号顺序不一致等问题。 展开更多
关键词 嵌入式系统 双口ram 1553B总线 数据覆盖
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轨道交通装备制造企业的RAMS体系分析
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作者 关欣 张博 马琳 《质量与认证》 2024年第1期93-95,共3页
本文以轨道交通装备制造企业RAMS体系为研究对象,论述了建立RAMS体系的必要性,分析了轨道交通装备制造企业RAMS体系管理组织、体系文件和审核等要素,对轨道交通装备制造企业RAMS体系落实情况进行了简要分析,为轨道交通装备制造企业完善R... 本文以轨道交通装备制造企业RAMS体系为研究对象,论述了建立RAMS体系的必要性,分析了轨道交通装备制造企业RAMS体系管理组织、体系文件和审核等要素,对轨道交通装备制造企业RAMS体系落实情况进行了简要分析,为轨道交通装备制造企业完善RAMS体系、建立切实有效的执行方法提供了参考。 展开更多
关键词 ramS体系 轨道交通装备制造 ramS体系管理组织 体系文件 ramS体系审核
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轨道交通车辆系统RAMS管理技术应用研讨
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作者 胡志强 《中国科技期刊数据库 工业A》 2024年第1期0182-0184,共3页
轨道交通是人们交通出行的主要方式之一,同时也是交通事业建设的重点内容,如何保证轨道交通车辆安全运行,做好车辆系统安全管理至关重要。RAMS管理属于主动性隐患管理工具,将其投入到轨道交通车辆系统中,能够及时发现和消除安全隐患,最... 轨道交通是人们交通出行的主要方式之一,同时也是交通事业建设的重点内容,如何保证轨道交通车辆安全运行,做好车辆系统安全管理至关重要。RAMS管理属于主动性隐患管理工具,将其投入到轨道交通车辆系统中,能够及时发现和消除安全隐患,最大程度上提升轨道交通车辆安全性和可靠性。尽管当前国内多数轨道交通车辆系统中开始运用RAMS管理进行可靠性、安全性管理,但却面临着相关主体单位的RAMS管理意识薄弱的问题,种种问题还有待进一步完善。本文就轨道交通车辆系统RAMS管理技术应用情况展开分析,并分析轨道交通车辆安全性、可靠性和可维修性内容,提出合理的控制手段付诸实践。 展开更多
关键词 ramS管理技术 轨道交通 车辆系统 可靠性 可维修性 安全性
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面向可变长度数据的高效ORAM方案
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作者 许道远 孙明明 李蓉 《工业信息安全》 2024年第1期32-40,共9页
数字经济的发展使得数据体量增长急剧加速,数据安全也成为了当前社会中重要的关注点。为了保护数据隐私安全,不经意随机访问机(ORAM,Oblivious Random Access Machine)方案被提出来保护不可信内存中的访问模式。本文提出一种全新的ORAM... 数字经济的发展使得数据体量增长急剧加速,数据安全也成为了当前社会中重要的关注点。为了保护数据隐私安全,不经意随机访问机(ORAM,Oblivious Random Access Machine)方案被提出来保护不可信内存中的访问模式。本文提出一种全新的ORAM协议,与以往只能存储固定长度分块的方案不同,本文提出的方案支持存储不同长度的数据,以更高的效率保证攻击者无法通过观察访问推测出隐私信息。该方案重塑了初始的二叉树结构,并采用加法同态加密方案来实现恒定的通信复杂性。该方案通过可变长度数据提升了多项性能,对于小型客户端存储,该ORAM协议的网络带宽比RingORAM低30%,比HIRBORAM低40%。同时,该ORAM协议极大地节省了客户端计算开销,并利用第三方(可信代理)提高了存储容量。最后证明了本方案过程中读取、写入以及驱逐操作的安全性。 展开更多
关键词 不经意随机访问机 隐私保护 数据安全 安全计算
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Pilot Study of Molecular Mechanism on Vasculogenic Mimicry in Bi-directional Differentiated Malignant Tumors 被引量:2
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作者 孙保存 张诗武 +2 位作者 赵秀兰 张丹芳 郝希山 《The Chinese-German Journal of Clinical Oncology》 CAS 2005年第1期50-52,68,共4页
Objective: To investigate the role of collagen IV and PAS positive substancesecreted by tumor cells in vasculogenic mimicry (VM) and the effects of VM on tumor cells expressingVEGF. Methods: 158 cases of bi-direction ... Objective: To investigate the role of collagen IV and PAS positive substancesecreted by tumor cells in vasculogenic mimicry (VM) and the effects of VM on tumor cells expressingVEGF. Methods: 158 cases of bi-direction differential malignant tumor specimens withparaffin-embedded were enrolled into our study and made tissue microarray which were dual-stainedwith CD31-PAS and stained with collagen IV. The difference of the areas and distribution withpattern surrounded by between CD31 and PAS positive respectively were identified via grid-counting,as well as the difference of VEGF expression with VE absent and present. Results: The basementmembrane of VM was both PAS and collagen IV positive. VEGF expression in the bi-directiondifferential malignant tumor was higher VM-absent than VM-present and the difference wasstatistically significance in malignant melanoma and alveolar rhabdomyosarcoma (P 【 0.05).Conclusion: PAS positive substance and collagen IV compose the wall of VE and VE could provide theoxygen and nutrition for tumor growth and progression. 展开更多
关键词 bi-direction differential malignant tumor vasculogenic mimicry dual-staining tissue microarray
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Multi-agent system application in accordance with game theory in bi-directional coordination network model 被引量:3
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作者 ZHANG Jie WANG Gang +3 位作者 YUE Shaohua SONG Yafei LIU Jiayi YAO Xiaoqiang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2020年第2期279-289,共11页
The multi-agent system is the optimal solution to complex intelligent problems. In accordance with the game theory, the concept of loyalty is introduced to analyze the relationship between agents' individual incom... The multi-agent system is the optimal solution to complex intelligent problems. In accordance with the game theory, the concept of loyalty is introduced to analyze the relationship between agents' individual income and global benefits and build the logical architecture of the multi-agent system. Besides, to verify the feasibility of the method, the cyclic neural network is optimized, the bi-directional coordination network is built as the training network for deep learning, and specific training scenes are simulated as the training background. After a certain number of training iterations, the model can learn simple strategies autonomously. Also,as the training time increases, the complexity of learning strategies rises gradually. Strategies such as obstacle avoidance, firepower distribution and collaborative cover are adopted to demonstrate the achievability of the model. The model is verified to be realizable by the examples of obstacle avoidance, fire distribution and cooperative cover. Under the same resource background, the model exhibits better convergence than other deep learning training networks, and it is not easy to fall into the local endless loop.Furthermore, the ability of the learning strategy is stronger than that of the training model based on rules, which is of great practical values. 展开更多
关键词 LOYALTY GAME theory bi-directional COORDINATION network MULTI-AGENT system learning STRATEGY
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Construction and analysis of a plant transformation binary vector pBDGG harboring a bi-directional promoter fusing dual visible reporter genes 被引量:4
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作者 Chunxiao Zhang Ying Gai +3 位作者 Wenqi Wang Yanyan Zhu Xuemei Chen Xiangning Jiang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第4期245-249,共5页
The constitutive promoter of cauliflower mosaic virus 35S (CaMV 35S) is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has... The constitutive promoter of cauliflower mosaic virus 35S (CaMV 35S) is a polar unidirectional promoter and is widely used in plant genetic engineering. In the present study, the unidirectional CaMV 35S promoter has been modified to a bi-directional promoter by fusing its minimal promoter element to the 5' end of CaMV 35S promoter in the opposite orientation. To qualitatively and quantitatively estimate its bi-directional transcriptional function and activity, two visible reporter genes, gusA (13-glucuronidase, GUS) and gfp (green fluorescent protein, GFP), were fused to the two ends of the promoter in bi-orientations ending with NOS terminator sequences, respectively. Stable expression of gusA and gfp genes in transgenic tobacco (Nicotiana tabacum L.) was visulized by histochemically staining for GUS and fluorescence microscopic observation under UV for GFP in transgenic plants. The expression of two reporter genes showed that the constructed bi-directional promoter did have the bi-directional transcriptional function in both expected orientations. The quantitative estimation of GUS and GFP were determined on a HITACHI F1000 Fluorescence Spectrophotometer with various wavelengths of excitation and emission. The GUS activity varied from g to 250 pmol 4-MU/min/mg protein and the GFP content varied from 0.9 to 1.8 μg/ mg protein in various lines of transgenic tobacco plants. Higher GUS activity generally coupled with lower GFP content, and vice versa. 展开更多
关键词 bi-directional promoter gusA gene gfp gene Nicotiana tabacum L. expression
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Experimental Investigation of Local Scour Around A New Pile-Group Foundation for Offshore Wind Turbines in Bi-Directional Current 被引量:5
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作者 JI Chao ZHANG Jin-feng +2 位作者 ZHANG Qing-he LI Ming-xing CHEN Tong-qing 《China Ocean Engineering》 SCIE EI CSCD 2018年第6期737-745,共9页
The local scour around a new pile-group foundation of offshore wind turbine subjected to a bi-directional current was physically modeled with a bi-directional flow flume. In a series of experiments, the flow velocity ... The local scour around a new pile-group foundation of offshore wind turbine subjected to a bi-directional current was physically modeled with a bi-directional flow flume. In a series of experiments, the flow velocity and topography of the seabed were measured based on a system composed of plane positioning equipment and an ADV.Experimental results indicate that the development of the scour hole was fast at the beginning, but then the scour rate decreased until reaching equilibrium. Erosion would occur around each pile of the foundation. In most cases, the scour pits were connected in pairs and the outside widths of the scour holes were larger than the inner widths. The maximum scour depth occurred at the side pile of the foundation for each test. In addition, a preliminary investigation shows that the larger the flow velocity, the larger the scour hole dimensions but the shorter equilibrium time. The field maximum scour depth around the foundation was obtained based on the physical experiments with the geometric length scales of 1:27.0, 1:42.5 and 1:68.0, and it agrees with the scour depth estimated by the HEC-18 equation. 展开更多
关键词 offshore wind turbines new pile-group foundation local scour bi-directional current
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