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Big Data 4.0: The Era of Big Intelligence
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作者 Zhaohao Sun 《Journal of Computer Science Research》 2024年第1期1-15,共15页
Big data has had significant impacts on our lives,economies,academia and industries over the past decade.The current equations are:What is the future of big data?What era do we live in?This article addresses these que... Big data has had significant impacts on our lives,economies,academia and industries over the past decade.The current equations are:What is the future of big data?What era do we live in?This article addresses these questions by looking at meta as an operation and argues that we are living in the era of big intelligence through analyzing from meta(big data)to big intelligence.More specifically,this article will analyze big data from an evolutionary perspective.The article overviews data,information,knowledge,and intelligence(DIKI)and reveals their relationships.After analyzing meta as an operation,this article explores Meta(DIKE)and its relationship.It reveals 5 Bigs consisting of big data,big information,big knowledge,big intelligence and big analytics.Applying meta on 5 Bigs,this article infers that 4 Big Data 4.0=meta(big data)=big intelligence.This article analyzes how intelligent big analytics support big intelligence.The proposed approach in this research might facilitate the research and development of big data,big data analytics,business intelligence,artificial intelligence,and data science. 展开更多
关键词 big Data 4.0 big analytics Business intelligence Artificial intelligence Data science
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Exploration and Practice of Big Data Introductory Courses for Big Data Management and Application Majors
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作者 Tinghui Huang Junchao Dong Liang Min 《Journal of Contemporary Educational Research》 2024年第2期131-137,共7页
As an introductory course for the emerging major of big data management and application,“Introduction to Big Data”has not yet formed a curriculum standard and implementation plan that is widely accepted and used by ... As an introductory course for the emerging major of big data management and application,“Introduction to Big Data”has not yet formed a curriculum standard and implementation plan that is widely accepted and used by everyone.To this end,we discuss some of our explorations and attempts in the construction and teaching process of big data courses for the major of big data management and application from the perspective of course planning,course implementation,and course summary.After interviews with students and feedback from questionnaires,students are highly satisfied with some of the teaching measures and programs currently adopted. 展开更多
关键词 big data management and application “Introduction to big Data” Teaching reform Curriculum exploration
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BIG评分对接受去骨瓣减压术的中重度创伤性脑损伤儿童早期脑功能的预测价值
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作者 徐静静 党红星 《临床医学进展》 2024年第4期2631-2640,共10页
目的:探讨BIG评分(由格拉斯哥评分、国际标准化比值、碱剩余组成)对接受去骨瓣减压术(DC)的中重度创伤性脑损伤(TBI)患儿脑功能早期预后的预测价值。方法:回顾性分析2014年3月至2023年7月于我院接受DC治疗的所有中重度TBI患儿,以出院时... 目的:探讨BIG评分(由格拉斯哥评分、国际标准化比值、碱剩余组成)对接受去骨瓣减压术(DC)的中重度创伤性脑损伤(TBI)患儿脑功能早期预后的预测价值。方法:回顾性分析2014年3月至2023年7月于我院接受DC治疗的所有中重度TBI患儿,以出院时儿童脑功能分类(PCPC)为结局,分为预后良好组(PCPC 1~2)和预后不良组(PCPC 3~6)。通过病历资料回顾,提取患儿的临床信息,并使用Logistic回归分析评估BIG评分的预测价值。结果:共纳入55例接受DC治疗的中重度TBI患儿,其中25例出院时脑功能良好,30例预后不良(包括9例死亡)。患儿入院时的高BIG评分(p < 0.001)、瞳孔对光反射差(p = 0.027),存在失血性休克(p = 0.042)及多发伤(p = 0.043)、脑水肿(p = 0.007),高血糖(p = 0.042)、高乳酸血症(p = 0.029)均与出院时脑功能不良相关。Logistic回归分析显示,入院时的高BIG评分是出院时脑功能不良的独立危险因素。ROC曲线分析确定的最佳BIG评分阈值为17.5,以此预测不良预后的敏感性为66.7%,特异性为88.0%。结论:接受DC的中重度TBI患儿出院时的总体脑功能不良比例为54.5%。入院时的BIG评分能够预测这些患儿出院时的早期脑功能预后,具有较高的敏感性和特异性。 展开更多
关键词 创伤性脑损伤 去骨瓣减压术 big评分 儿童 预后
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Reliability evaluation of IGBT power module on electric vehicle using big data
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作者 Li Liu Lei Tang +5 位作者 Huaping Jiang Fanyi Wei Zonghua Li Changhong Du Qianlei Peng Guocheng Lu 《Journal of Semiconductors》 EI CAS CSCD 2024年第5期50-60,共11页
There are challenges to the reliability evaluation for insulated gate bipolar transistors(IGBT)on electric vehicles,such as junction temperature measurement,computational and storage resources.In this paper,a junction... There are challenges to the reliability evaluation for insulated gate bipolar transistors(IGBT)on electric vehicles,such as junction temperature measurement,computational and storage resources.In this paper,a junction temperature estimation approach based on neural network without additional cost is proposed and the lifetime calculation for IGBT using electric vehicle big data is performed.The direct current(DC)voltage,operation current,switching frequency,negative thermal coefficient thermistor(NTC)temperature and IGBT lifetime are inputs.And the junction temperature(T_(j))is output.With the rain flow counting method,the classified irregular temperatures are brought into the life model for the failure cycles.The fatigue accumulation method is then used to calculate the IGBT lifetime.To solve the limited computational and storage resources of electric vehicle controllers,the operation of IGBT lifetime calculation is running on a big data platform.The lifetime is then transmitted wirelessly to electric vehicles as input for neural network.Thus the junction temperature of IGBT under long-term operating conditions can be accurately estimated.A test platform of the motor controller combined with the vehicle big data server is built for the IGBT accelerated aging test.Subsequently,the IGBT lifetime predictions are derived from the junction temperature estimation by the neural network method and the thermal network method.The experiment shows that the lifetime prediction based on a neural network with big data demonstrates a higher accuracy than that of the thermal network,which improves the reliability evaluation of system. 展开更多
关键词 IGBT junction temperature neural network electric vehicles big data
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Big Data Access Control Mechanism Based on Two-Layer Permission Decision Structure
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作者 Aodi Liu Na Wang +3 位作者 Xuehui Du Dibin Shan Xiangyu Wu Wenjuan Wang 《Computers, Materials & Continua》 SCIE EI 2024年第4期1705-1726,共22页
Big data resources are characterized by large scale, wide sources, and strong dynamics. Existing access controlmechanisms based on manual policy formulation by security experts suffer from drawbacks such as low policy... Big data resources are characterized by large scale, wide sources, and strong dynamics. Existing access controlmechanisms based on manual policy formulation by security experts suffer from drawbacks such as low policymanagement efficiency and difficulty in accurately describing the access control policy. To overcome theseproblems, this paper proposes a big data access control mechanism based on a two-layer permission decisionstructure. This mechanism extends the attribute-based access control (ABAC) model. Business attributes areintroduced in the ABAC model as business constraints between entities. The proposed mechanism implementsa two-layer permission decision structure composed of the inherent attributes of access control entities and thebusiness attributes, which constitute the general permission decision algorithm based on logical calculation andthe business permission decision algorithm based on a bi-directional long short-term memory (BiLSTM) neuralnetwork, respectively. The general permission decision algorithm is used to implement accurate policy decisions,while the business permission decision algorithm implements fuzzy decisions based on the business constraints.The BiLSTM neural network is used to calculate the similarity of the business attributes to realize intelligent,adaptive, and efficient access control permission decisions. Through the two-layer permission decision structure,the complex and diverse big data access control management requirements can be satisfied by considering thesecurity and availability of resources. Experimental results show that the proposed mechanism is effective andreliable. In summary, it can efficiently support the secure sharing of big data resources. 展开更多
关键词 big data access control data security BiLSTM
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Study of primordial deuterium abundance in Big Bang nucleosynthesis
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作者 Zhi-Lin Shen Jian-Jun He 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第3期208-215,共8页
Big Bang nucleosynthesis(BBN)theory predicts the primordial abundances of the light elements^(2) H(referred to as deuterium,or D for short),^(3)He,^(4)He,and^(7) Li produced in the early universe.Among these,deuterium... Big Bang nucleosynthesis(BBN)theory predicts the primordial abundances of the light elements^(2) H(referred to as deuterium,or D for short),^(3)He,^(4)He,and^(7) Li produced in the early universe.Among these,deuterium,the first nuclide produced by BBN,is a key primordial material for subsequent reactions.To date,the uncertainty in predicted deuterium abundance(D/H)remains larger than the observational precision.In this study,the Monte Carlo simulation code PRIMAT was used to investigate the sensitivity of 11 important BBN reactions to deuterium abundance.We found that the reaction rate uncertainties of the four reactions d(d,n)^(3)He,d(d,p)t,d(p,γ)^(3)He,and p(n,γ)d had the largest influence on the calculated D/H uncertainty.Currently,the calculated D/H uncertainty cannot reach observational precision even with the recent LUNA precise d(p,γ)^(3) He rate.From the nuclear physics aspect,there is still room to largely reduce the reaction-rate uncertainties;hence,further measurements of the important reactions involved in BBN are still necessary.A photodisintegration experiment will be conducted at the Shanghai Laser Electron Gamma Source Facility to precisely study the deuterium production reaction of p(n,γ)d. 展开更多
关键词 big Bang nucleosynthesis Abundance of deuterium Reaction cross section Reaction rate Monte Carlo method
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Evaluation of a software positioning tool to support SMEs in adoption of big data analytics
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作者 Matthew Willetts Anthony S.Atkins 《Journal of Electronic Science and Technology》 EI CAS CSCD 2024年第1期13-24,共12页
Big data analytics has been widely adopted by large companies to achieve measurable benefits including increased profitability,customer demand forecasting,cheaper development of products,and improved stock control.Sma... Big data analytics has been widely adopted by large companies to achieve measurable benefits including increased profitability,customer demand forecasting,cheaper development of products,and improved stock control.Small and medium sized enterprises(SMEs)are the backbone of the global economy,comprising of 90%of businesses worldwide.However,only 10%SMEs have adopted big data analytics despite the competitive advantage they could achieve.Previous research has analysed the barriers to adoption and a strategic framework has been developed to help SMEs adopt big data analytics.The framework was converted into a scoring tool which has been applied to multiple case studies of SMEs in the UK.This paper documents the process of evaluating the framework based on the structured feedback from a focus group composed of experienced practitioners.The results of the evaluation are presented with a discussion on the results,and the paper concludes with recommendations to improve the scoring tool based on the proposed framework.The research demonstrates that this positioning tool is beneficial for SMEs to achieve competitive advantages by increasing the application of business intelligence and big data analytics. 展开更多
关键词 big data analytics EVALUATION Small and medium sized enterprises (SMEs) Strategic framework
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Big data challenge for monitoring quality in higher education institutions using business intelligence dashboards
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作者 Ali Sorour Anthony S.Atkins 《Journal of Electronic Science and Technology》 EI CAS CSCD 2024年第1期25-41,共17页
As big data becomes an apparent challenge to handle when building a business intelligence(BI)system,there is a motivation to handle this challenging issue in higher education institutions(HEIs).Monitoring quality in H... As big data becomes an apparent challenge to handle when building a business intelligence(BI)system,there is a motivation to handle this challenging issue in higher education institutions(HEIs).Monitoring quality in HEIs encompasses handling huge amounts of data coming from different sources.This paper reviews big data and analyses the cases from the literature regarding quality assurance(QA)in HEIs.It also outlines a framework that can address the big data challenge in HEIs to handle QA monitoring using BI dashboards and a prototype dashboard is presented in this paper.The dashboard was developed using a utilisation tool to monitor QA in HEIs to provide visual representations of big data.The prototype dashboard enables stakeholders to monitor compliance with QA standards while addressing the big data challenge associated with the substantial volume of data managed by HEIs’QA systems.This paper also outlines how the developed system integrates big data from social media into the monitoring dashboard. 展开更多
关键词 big data Business intelligence(BI) Dashboards Higher education(HE) Quality assurance(QA) Social media
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The Impact of Big Five Personality Traits on Older Europeans’ Physical Health
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作者 Eleni Serafetinidou Christina Parpoula 《Journal of Biomedical Science and Engineering》 2024年第2期41-56,共16页
Investigating the role of Big Five personality traits in relation to various health outcomes has been extensively studied. The impact of “Big Five” on physical health is here explored for older Europeans with a focu... Investigating the role of Big Five personality traits in relation to various health outcomes has been extensively studied. The impact of “Big Five” on physical health is here explored for older Europeans with a focus on examining age groups differences. The study sample included 378,500 respondents derived from the seventh data wave of Survey of Health, Aging and Retirement in Europe (SHARE). The physical health status of older Europeans was estimated by constructing an index considering the combined effect of well-established health indicators such as the number of chronic diseases, mobility limitations, limitations with basic and instrumental activities of daily living, and self-perceived health. This index was used for an overall physical health assessment, for which the higher the score for an individual, the worst health level. Then, through a dichotomization process applied to the retrieved Principal Component Analysis scores, a two-group discrimination (good or bad health status) of SHARE participants was obtained as regards their physical health condition, allowing for further con-structing logistic regression models to assess the predictive significance of “Big Five” and their protective role for physical health. Results showed that neuroti-cism was the most significant predictor of physical health for all age groups un-der consideration, while extraversion, agreeableness and openness were not found to significantly affect the self-reported physical health levels of midlife adults aged 50 up to 64. Older adults aged 65 up to 79 were more prone to open-ness, whereas the oldest old individuals aged 80 up to 105 were mainly affected by openness and conscientiousness. . 展开更多
关键词 big Five Personality Traits Physical Health Older Europeans SHARE Principal Component Analysis
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Sports Prediction Model through Cloud Computing and Big Data Based on Artificial Intelligence Method
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作者 Aws I. Abu Eid Achraf Ben Miled +9 位作者 Ahlem Fatnassi Majid A. Nawaz Ashraf F. A. Mahmoud Faroug A. Abdalla Chams Jabnoun Aida Dhibi Firas M. Allan Mohammed Ahmed Elhossiny Salem Belhaj Imen Ben Mohamed 《Journal of Intelligent Learning Systems and Applications》 2024年第2期53-79,共27页
This article delves into the intricate relationship between big data, cloud computing, and artificial intelligence, shedding light on their fundamental attributes and interdependence. It explores the seamless amalgama... This article delves into the intricate relationship between big data, cloud computing, and artificial intelligence, shedding light on their fundamental attributes and interdependence. It explores the seamless amalgamation of AI methodologies within cloud computing and big data analytics, encompassing the development of a cloud computing framework built on the robust foundation of the Hadoop platform, enriched by AI learning algorithms. Additionally, it examines the creation of a predictive model empowered by tailored artificial intelligence techniques. Rigorous simulations are conducted to extract valuable insights, facilitating method evaluation and performance assessment, all within the dynamic Hadoop environment, thereby reaffirming the precision of the proposed approach. The results and analysis section reveals compelling findings derived from comprehensive simulations within the Hadoop environment. These outcomes demonstrate the efficacy of the Sport AI Model (SAIM) framework in enhancing the accuracy of sports-related outcome predictions. Through meticulous mathematical analyses and performance assessments, integrating AI with big data emerges as a powerful tool for optimizing decision-making in sports. The discussion section extends the implications of these results, highlighting the potential for SAIM to revolutionize sports forecasting, strategic planning, and performance optimization for players and coaches. The combination of big data, cloud computing, and AI offers a promising avenue for future advancements in sports analytics. This research underscores the synergy between these technologies and paves the way for innovative approaches to sports-related decision-making and performance enhancement. 展开更多
关键词 Artificial Intelligence Machine Learning Spark Apache big Data SAIM
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Particle Swarm Optimization-Based Hyperparameters Tuning of Machine Learning Models for Big COVID-19 Data Analysis
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作者 Hend S. Salem Mohamed A. Mead Ghada S. El-Taweel 《Journal of Computer and Communications》 2024年第3期160-183,共24页
Analyzing big data, especially medical data, helps to provide good health care to patients and face the risks of death. The COVID-19 pandemic has had a significant impact on public health worldwide, emphasizing the ne... Analyzing big data, especially medical data, helps to provide good health care to patients and face the risks of death. The COVID-19 pandemic has had a significant impact on public health worldwide, emphasizing the need for effective risk prediction models. Machine learning (ML) techniques have shown promise in analyzing complex data patterns and predicting disease outcomes. The accuracy of these techniques is greatly affected by changing their parameters. Hyperparameter optimization plays a crucial role in improving model performance. In this work, the Particle Swarm Optimization (PSO) algorithm was used to effectively search the hyperparameter space and improve the predictive power of the machine learning models by identifying the optimal hyperparameters that can provide the highest accuracy. A dataset with a variety of clinical and epidemiological characteristics linked to COVID-19 cases was used in this study. Various machine learning models, including Random Forests, Decision Trees, Support Vector Machines, and Neural Networks, were utilized to capture the complex relationships present in the data. To evaluate the predictive performance of the models, the accuracy metric was employed. The experimental findings showed that the suggested method of estimating COVID-19 risk is effective. When compared to baseline models, the optimized machine learning models performed better and produced better results. 展开更多
关键词 big COVID-19 Data Machine Learning Hyperparameter Optimization Particle Swarm Optimization Computational Intelligence
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Construction and Application Exploration of Smart Agriculture Based on Big Data Technology
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作者 Zhongling Li Hairui Wang 《Journal of Electronic Research and Application》 2024年第3期72-77,共6页
Big data finds extensive application and many fields.It brings new opportunities for the development of agriculture.Using big data technology to promote the development of smart agriculture can greatly improve the eff... Big data finds extensive application and many fields.It brings new opportunities for the development of agriculture.Using big data technology to promote the development of smart agriculture can greatly improve the effect of agricultural planting,reduce the input of manpower and material resources,and lay a solid foundation for the realization of agricultural modernization.In this regard,this paper briefly analyzes the construction and application of smart agriculture based on big data technology,hoping to provide some valuable insights for readers. 展开更多
关键词 big data technology Smart agriculture CONSTRUCTION Apply
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Digital Transformation of Enterprise Human Resource Management Enabled by Big Data
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作者 Zhefan Zhuang 《Proceedings of Business and Economic Studies》 2024年第2期60-65,共6页
With the continuous development of big data technology,the digital transformation of enterprise human resource management has become a development trend.Human resources is one of the most important resources of enterp... With the continuous development of big data technology,the digital transformation of enterprise human resource management has become a development trend.Human resources is one of the most important resources of enterprises,which is crucial to the competitiveness of enterprises.Enterprises need to attract,retain,and motivate excellent employees,thereby enhancing the innovation ability of enterprises and improving competitiveness and market share in the market.To maintain advantages in the fierce market competition,enterprises need to adopt more scientific and effective human resource management methods to enhance organizational efficiency and competitiveness.At the same time,this paper analyzes the dilemma faced by enterprise human resource management,points out the new characteristics of enterprise human resource management enabled by big data,and puts forward feasible suggestions for enterprise digital transformation. 展开更多
关键词 big data Digital transformation Enterprise management Human resource management
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Opportunities and Challenges of College Mental Health Education from the Perspective of Big Data
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作者 Xiaojian Cai 《Journal of Contemporary Educational Research》 2024年第4期193-198,共6页
This paper explores the opportunities and challenges of college mental health education from the perspective of big data.Firstly,through literature review,the importance of mental health education and the current issu... This paper explores the opportunities and challenges of college mental health education from the perspective of big data.Firstly,through literature review,the importance of mental health education and the current issues are elucidated.Then,from the perspective of big data,the potential opportunities of big data in college mental health education are analyzed,including data-driven personalized education,real-time monitoring and warning systems,and interdisciplinary research and collaboration.At the same time,the challenges faced by college mental health education under the perspective of big data are also pointed out,such as data privacy and security issues,insufficient data analysis and interpretation capabilities,and inadequate technical facilities and talent support.Lastly,the research content of this paper is summarized,and directions and suggestions for future research are proposed. 展开更多
关键词 big data perspective College mental health education OPPORTUNITIES CHALLENGES Personalized education Real-time monitoring Interdisciplinary research
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A Review of the Status and Development Strategies of Computer Science and Technology Under the Background of Big Data
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作者 Junlin Zhang 《Journal of Electronic Research and Application》 2024年第2期49-53,共5页
This article discusses the current status and development strategies of computer science and technology in the context of big data.Firstly,it explains the relationship between big data and computer science and technol... This article discusses the current status and development strategies of computer science and technology in the context of big data.Firstly,it explains the relationship between big data and computer science and technology,focusing on analyzing the current application status of computer science and technology in big data,including data storage,data processing,and data analysis.Then,it proposes development strategies for big data processing.Computer science and technology play a vital role in big data processing by providing strong technical support. 展开更多
关键词 big data Computer science and technology Data storage Data processing Data visualization
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Designing and Implementing an Advanced Big Data Governance Platform
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作者 Yekun Chen Tianqi Xu Yongjiang Xue 《Journal of Electronic Research and Application》 2024年第3期13-19,共7页
Contemporary mainstream big data governance platforms are built atop the big data ecosystem components,offering a one-stop development and analysis governance platform for the collection,transmission,storage,cleansing... Contemporary mainstream big data governance platforms are built atop the big data ecosystem components,offering a one-stop development and analysis governance platform for the collection,transmission,storage,cleansing,transformation,querying and analysis,data development,publishing,and subscription,sharing and exchange,management,and services of massive data.These platforms serve various role members who have internal and external data needs.However,in the era of big data,the rapid update and iteration of big data technologies,the diversification of data businesses,and the exponential growth of data present more challenges and uncertainties to the construction of big data governance platforms.This paper discusses how to effectively build a data governance platform under the big data system from the perspectives of functional architecture,logical architecture,data architecture,and functional design. 展开更多
关键词 big data Data governance Cleansing and transformation Data development Sharing and exchange
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Research on the Development Strategy of Smart Tourism in Hainan Under the Background of Big Data
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作者 Limei Fu 《Journal of Contemporary Educational Research》 2024年第4期108-113,共6页
Hainan is a major tourist province.It is urgent to promote the transformation and upgrading of Hainan’s tourism industry from a traditional service industry to a modern service industry by means of informatization.Sm... Hainan is a major tourist province.It is urgent to promote the transformation and upgrading of Hainan’s tourism industry from a traditional service industry to a modern service industry by means of informatization.Smart tourism is a brand-new tourism form and operation mode of tourism transformation and upgrading.Integrating big data technology will make smart tourism more accurate in three aspects:tourism management,tourism service,and tourism marketing,and further enhance the satisfaction of the tourism experience.This paper studies the development status of smart tourism in Hainan,deeply summarizes its existing problems and causes,and puts forward the development strategy of smart tourism in Hainan to promote the healthy development of the tourism industry in Hainan. 展开更多
关键词 big data Smart tourism Development strategy
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Research on Tax Risk Regulation and Strategic Management in the Context of Big Data
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作者 Shouzeng Gong 《Proceedings of Business and Economic Studies》 2024年第2期145-150,共6页
With the rapid development of big data,big data has been more and more applied in all walks of life.Under the big data environment,massive big data provides convenience for regional tax risk control and strategic deci... With the rapid development of big data,big data has been more and more applied in all walks of life.Under the big data environment,massive big data provides convenience for regional tax risk control and strategic decision-making but also increases the difficulty of data supervision and management.By analyzing the status quo of big data and tax risk management,this paper finds many problems and puts forward effective countermeasures for tax risk supervision and strategic management by using big data. 展开更多
关键词 big data Tax risk Strategic management
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Research on Enterprise Human Resource Management Reform Strategy in the Era of Big Data
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作者 Xin Zhang 《Proceedings of Business and Economic Studies》 2024年第2期184-190,共7页
In the 21st century,with the development of the Internet,mobile devices,and information technology,society has entered a new era:the era of big data.With the help of big data technology,enterprises can obtain massive ... In the 21st century,with the development of the Internet,mobile devices,and information technology,society has entered a new era:the era of big data.With the help of big data technology,enterprises can obtain massive market and consumer data,realize in-depth analysis of business and market,and enable enterprises to have a deeper understanding of consumer needs,preferences,and behaviors.At the same time,big data technology can also help enterprises carry out human resource management innovation and improve the performance and competitiveness of enterprises.Of course,from another perspective,enterprises in this era are also facing severe challenges.In the face of massive data processing and analysis,it requires superb data processing and analysis capabilities.Secondly,enterprises need to reconstruct their management system to adapt to the changes in the era of big data.Enterprises must treat data as assets and establish a perfect data management system.In addition,enterprises also need to pay attention to protecting customer privacy and data security to avoid data leakage and abuse.In this context,this paper will explore the thinking of enterprise human resource management innovation in the era of big data,and put forward some suggestions on enterprise human resource management innovation. 展开更多
关键词 big data Enterprise human resource management Reform strategy
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Big-BALB/c小鼠亚系选育前后的抗体制备效率比较研究
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作者 王丹 张晓璐 +10 位作者 王妍 傅博 王文栋 刘京 张甦寅 武怡荷 吴德国 杜小燕 战大伟 章秀林 李长龙 《实验动物与比较医学》 CAS 2023年第6期612-618,共7页
目的比较利用BALB/c和Big-BALB/c(简称B-BALB/c)两种亚系小鼠制备鼠痘及鼠肝炎单克隆抗体的效率,为今后通过杂交瘤进行体内诱导单克隆抗体制备时实验动物的选择提供参考。方法从常规繁育的BALB/c小鼠群体中选择4周龄体重超过正常动物5%... 目的比较利用BALB/c和Big-BALB/c(简称B-BALB/c)两种亚系小鼠制备鼠痘及鼠肝炎单克隆抗体的效率,为今后通过杂交瘤进行体内诱导单克隆抗体制备时实验动物的选择提供参考。方法从常规繁育的BALB/c小鼠群体中选择4周龄体重超过正常动物5%(后期选种标准为超过10%)的个体进行选种并单独繁育,经10代选育制备B-BALB/c小鼠亚系。选择10~11周龄、雌雄各半的BALB/c小鼠和B-BALB/c小鼠各40只,分别接种用液体石蜡预处理后的鼠痘单克隆抗体杂交瘤细胞株G23或鼠肝炎单克隆抗体杂交瘤细胞株Y15,然后通过体内诱生法获取含单克隆抗体的小鼠腹水,利用间接ELISA法检验抗体效价,按照品系、性别和接种杂交瘤类型对小鼠进行分组,分析腹水产量、抗体效价和抗体产量以评估两种亚系小鼠的抗体制备效率。结果经10代选育后获得的10周龄雄性和雌性B-BALB/c小鼠体重分别比同周龄BALB/c小鼠增加了22.3%和12.8%。抗体制备过程中,B-BALB/c小鼠的耐受力比BALB/c小鼠更好,能够适应二次腹水采集;与BALB/c小鼠相比,B-BALB/c小鼠腹水产量和抗体效价均显著提高,尤以杂交瘤细胞株G23接种组更明显(均P<0.0001)。接种杂交瘤细胞株G23或Y15后,B-BALB/c小鼠的平均抗体产量(14.99×10^(4)U和33.22×10^(4)U)高于BALB/c小鼠(5.33×10^(4)U和19.31×10^(4)U)(均P<0.01)。接种杂交瘤细胞株G23后,B-BALB/c小鼠的平均单位体重的抗体产量(0.55×10^(4)U/g)高于BALB/c小鼠(0.23×10^(4)U/g)(P<0.0001)。接种杂交瘤细胞株Y15后,雌性B-BALB/c和BALB/c小鼠的单位体重抗体产量高于同亚系的雄性B-BALB/c小鼠(均P<0.01)。结论B-BALB/c小鼠在单克隆抗体体内诱导制备中可作为BALB/c小鼠的替代选择,能够达到减少实验动物使用数量、降低人力成本并提高抗体制备效率的目的。 展开更多
关键词 单克隆抗体 制备效率 杂交瘤 BALB/C小鼠 big-BALB/c小鼠
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