This paper reviews a novel cell-based biosensor and Bio-MEMS which incorporate living cells as sensing elements that convert a change in immediate environment to signals conducive for processing.It is characterized wi...This paper reviews a novel cell-based biosensor and Bio-MEMS which incorporate living cells as sensing elements that convert a change in immediate environment to signals conducive for processing.It is characterized with high sensitivity,excellent selectivity and fast response and have been implemented for a number of applications ranging from pharmaceutical screening to environmental pollutant detection.This paper also introduces our recent work about Light-Addressable Potentiometric Sensors (LAPS),Field Effect Transistor (FET),Micro-Electrode Array Sensors (MEAS) and Bio-MEMS for detecting the changes of concentration of extracellular ions and the action potential of living cell under effect of drugs and environmental parameters.Finely, the paper gives some prospects of cell-based biosensors in the future.展开更多
Extracellular matrix(ECM)-based implantable neural electrodes(NEs)were achieved using a microfabrication strategy on naturalsubstrate-based organic materials.The ECM-based design minimized the introduction of non-natu...Extracellular matrix(ECM)-based implantable neural electrodes(NEs)were achieved using a microfabrication strategy on naturalsubstrate-based organic materials.The ECM-based design minimized the introduction of non-natural products into the brain.Further,it rendered the implants sufficiently rigid for penetration into the target brain region and allowed them subsequently to soften to match the elastic modulus of brain tissue upon exposure to physiological conditions,thereby reducing inflammatory strain fields in the tissue.Preliminary studies suggested that ECM-NEs produce a reduced inflammatory response compared with inorganic rigid and flexible approaches.In vivo intracortical recordings from the rat motor cortex illustrate one mode of use for these ECM-NEs.展开更多
基金Acknowledgement： This work was supported by the National Natural Science Foundation of China （Grant Nos. 30270387, No. 30570492）；the Project of State Key Laboratory of Transducer Technology of China （Grant No. SKT0403）；the Foundation for the Bureau of Zhejiang Province of China （Grant No. 20040197）.
文摘This paper reviews a novel cell-based biosensor and Bio-MEMS which incorporate living cells as sensing elements that convert a change in immediate environment to signals conducive for processing.It is characterized with high sensitivity,excellent selectivity and fast response and have been implemented for a number of applications ranging from pharmaceutical screening to environmental pollutant detection.This paper also introduces our recent work about Light-Addressable Potentiometric Sensors (LAPS),Field Effect Transistor (FET),Micro-Electrode Array Sensors (MEAS) and Bio-MEMS for detecting the changes of concentration of extracellular ions and the action potential of living cell under effect of drugs and environmental parameters.Finely, the paper gives some prospects of cell-based biosensors in the future.
基金This work was funded by the Defense Advanced Research Projects Agency(DARPA)MTO under the auspices of Dr.Jack Judy through the Space and Naval Warfare Systems Center,Pacific Grant/Contract No.N66001-11-1-4014.
文摘Extracellular matrix(ECM)-based implantable neural electrodes(NEs)were achieved using a microfabrication strategy on naturalsubstrate-based organic materials.The ECM-based design minimized the introduction of non-natural products into the brain.Further,it rendered the implants sufficiently rigid for penetration into the target brain region and allowed them subsequently to soften to match the elastic modulus of brain tissue upon exposure to physiological conditions,thereby reducing inflammatory strain fields in the tissue.Preliminary studies suggested that ECM-NEs produce a reduced inflammatory response compared with inorganic rigid and flexible approaches.In vivo intracortical recordings from the rat motor cortex illustrate one mode of use for these ECM-NEs.
