Manure substitution improves maize yield by promoting soil fertility and mediating the microbial community in lime concretion black soil

Synthetic nitrogen(N)fertilizer has made a great contribution to the improvement of soil fertility and productivity,but excessive application of synthetic N fertilizer may cause agroecosystem risks,such as soil acidification,groundwater contamination and biodiversity reduction.Meanwhile,organic substitution has received increasing attention for its ecologically and environmentally friendly and productivity benefits.However,the linkages between manure substitution,crop yield and the underlying microbial mechanisms remain uncertain.To bridge this gap,a three-year field experiment was conducted with five fertilization regimes:i)Control,non-fertilization;CF,conventional synthetic fertilizer application;CF_(1/2)M_(1/2),1/2 N input via synthetic fertilizer and 1/2 N input via manure;CF_(1/4)M_(3/4),1/4 N input synthetic fertilizer and 3/4 N input via manure;M,manure application.All fertilization treatments were designed to have equal N input.Our results showed that all manure substituted treatments achieved high soil fertility indexes(SFI)and productivities by increasing the soil organic carbon(SOC),total N(TN)and available phosphorus(AP)concentrations,and by altering the bacterial community diversity and composition compared with CF.SOC,AP,and the soil C:N ratio were mainly responsible for microbial community variations.The co-occurrence network revealed that SOC and AP had strong positive associations with Rhodospirillales and Burkholderiales,while TN and C:N ratio had positive and negative associations with Micromonosporaceae,respectively.These specific taxa are implicated in soil macroelement turnover.Random Forest analysis predicted that both biotic(bacterial composition and Micromonosporaceae)and abiotic(AP,SOC,SFI,and TN)factors had significant effects on crop yield.The present work strengthens our understanding of the effects of manure substitution on crop yield and provides theoretical support for optimizing fertilization strategies.

Co-incorporating green manure and crop straw increases crop productivity and improves soil quality with low greenhouse-gas emissions in a crop rotation

In a nine-year field experiment in a wheat-maize-sunflower cropping system in Hetao Irrigation Area,Inner Mongolia,China,organic amendments applied as straw,manure,green manure,and the combination of green manure and straw increased wheat and maize yield,soil aggregate stability,and soil microbial activity in comparison with chemical fertilizer,without changing greenhouse gas emission intensity.

Application of organic manure as a potential strategy to alleviate the limitation of microbial resources in soybean rhizospheric and bulk soils

The development and vigor of soil microorganisms in terrestrial ecosystems are frequently constrained by the limited availability of essential elements such as carbon(C),nitrogen(N),and phosphorus(P).In this study,we investigated the impact of long-term application of varying levels of organic manure,low(7.5 Mg ha^(−1)yr^(−1)),moderate(15.0 Mg ha^(−1)yr^(−1)),and high(22.5 Mg ha^(−1)yr^(−1)),on the stoichiometry of enzymes and the structures of the microbial communities in soybean rhizospheric and bulk soils.The main goal of this research was to examine how soil microbial resource limitations in the rhizosphere respond to different long-term fertilization strategies.The soil enzymatic activities were quantified,and the structure of the microbial community was assessed by analyzing phospholipid fatty acid profiles.When compared to the bulk soil,the rhizospheric soil had significant increases in microbial biomass carbon(MBC),nitrogen(MBN),and phosphorus(MBP),with MBC increasing by 54.19 to 72.86%,MBN by 47.30 to 48.17%,and MBP by 17.37 to 208.47%.Compared with the unfertilized control(CK),the total microbial biomasses of the rhizospheric(increased by 22.80 to 90.82%)and bulk soils(increased by 10.57 to 60.54%)both exhibited increases with the application of organic manure,and the rhizospheric biomass was higher than that of bulk soil.Compared with bulk soil,the activities of C-,N-and P-acquiring enzymes of rhizospheric soil increased by 22.49,14.88,and 29.45%under high levels of organic manure,respectively.Analyses of vector length,vector angle,and scatter plots revealed that both rhizospheric and bulk soils exhibited limitations in terms of both carbon(C)and phosphorus(P)availability.The results of partial least-squares path modelling indicated that the rhizospheric soil exhibited a more pronounced response to the rate of manure application than the bulk soil.The varying reactions of rhizospheric and bulk soils to the extended application of organic manure underscore the crucial function of the rhizosphere in mitigating limitations related to microbial resources,particularly in the context of different organic manure application rates.

Detection of Adenovirus in Fresh Fruit, Vegetables, Wastewater and Manure from Irrigated Farms in Ouagadougou, Burkina Faso

Enteric viral pathogens are responsible for numerous epidemics associated with the consumption of fresh fruit and vegetable, whether raw or minimally processed. The aim of the present study was to assess agricultural practices and the presence of adenovirus (AdV) in fruits and vegetables, manure and irrigation wastewater sampled in the urban and peri-urban perimeters of Ouagadougou. A total of 286 samples including 30 lettuces, 42 tomatoes, 30 carrots, 30 strawberries, 74 manures and 80 wastewater samples were collected from four market garden sites in and around Ouagadougou. Nested PCR was performed with specific primers to detect adenoviruses (AdVs). A face-to-face survey was carried out using a questionnaire on market garden production practices. Overall, adenoviruses prevalence was 5.9% [IC95, 3.2% - 8.7%] in all samples analyzed. It was specifically 7.14% (3/42) from tomatoes, 6.7% (2/30) from lettuces, 20% (6/30) on strawberries and 7.5% (6/80) in irrigation water. The survey showed that irrigation water came from untreated sources (dam, well, canal) and then 52% of farms used untreated manure. No farms have implemented measures to limit access by domestic and wild animals. This work shows the presence of human adenoviruses in surface irrigation water and fresh produce, which is of concern when fresh produce is consumed raw. To reduce the public health risks associated with consuming these foods, it is essential to follow good hygiene and cultivation practices.

Excessive manure application stimulates nitrogen cycling but only weakly promotes crop yields in an acidic Ultisol:Results from a 20-year field experiment

Population growth and growing demand for livestock products produce large amounts of manure,which can be harnessed to maintain soil sustainability and crop productivity.However,the impacts of excessive manure application on crop yields,nitrogen(N)-cycling processes and microorganisms remain unknown.Here,we explored the effects of 20-year of excessive rates(18 and 27 Mg ha^(–1)yr^(–1))of pig manure application on peanut crop yields,soil nutrient contents,N-cycling processes and the abundance of N-cycling microorganisms in an acidic Ultisol in summer and winter,compared with none and a regular rate(9 Mg ha^(–1)yr^(–1))of pig manure application.Long-term excessive pig manure application,especially at the high-rate,significantly increased soil nutrient contents,the abundance of N-cycling functional genes,potential nitrification and denitrification activity,while it had a weaker effect on peanut yield and plant biomass.Compared with manure application,seasonality had a much weaker effect on N-cycling gene abundance.Random forest analysis showed that available phosphorus(AP)content was the primary predictor for N-cycling gene abundance,with significant and positive associations with all tested N-cycling genes.Our study clearly illustrated that excessive manure application would increase N-cycling gene abundance and potential N loss with relatively weak promotion of crop yields,providing significant implications for sustainable agriculture in the acidic Ultisols.

The combined effects of farm yard manure and boron application on growth,and oil quality of Canola grown under newly reclaimed soils

Two field experiments were conducted during the main seasons of 2021/2022 at the Research and Production Station of National Research Centre in Egypt to investigate the effects of farmyard manure(FYM)and boron on Canola growth,yield,oil yield,and quality.The results unequivocally demonstrated that the combined application of FYM at a rate of 14.4 ton ha^(-1)with a foliar spray of boron at 100 ppm positively influenced plant characteristics,leading to enhanced growth rates and higher yields compared to the control group.Moreover,this integrated approach significantly improved nutrient content by enhancing levels of oil content,carbohydrates,proteins,phenolics,flavonoids,and total soluble sugars.These findings provide compelling evidence that utilizing farm manure along with boron can effectively enhance Canola properties in newly reclaimed soils while promoting sustainable agricultural practices.

Legume green manure can intensify the function of chemical nitrogen fertilizer substitution via increasing nitrogen supply and uptake of wheat

Achieving the green development of agriculture requires the reduction of chemical nitrogen(N)fertilizer input.Previous studies have confirmed that returning green manure to the field is an effective measure to improve crop yields while substituting partial chemical N fertilizer.However,it remains unclear how to further intensify the substituting function of green manure and elucidate its underlying agronomic mechanism.In a split-plot field experiment in spring wheat,different green manures returned to the field under reduced chemical N supply was established in an oasis area since 2018,in order to investigate the effect of green manure and reduced N on grain yield,N uptake,N use efficiency(NUE),N nutrition index,soil organic matter,and soil N of wheat in 2020-2022.Our results showed that mixed sown common vetch and hairy vetch can substitute 40%of chemical N fertilizer without reducing grain yield or N accumulation.Noteworthily,mixed sown common vetch and hairy vetch under reduced N by 20%showed the highest N agronomy efficiency and recovery efficiency,which were 92.0%and 46.0%higher than fallow after wheat harvest and conventional N application rate,respectively.The increase in NUE of wheat was mainly attributed to mixed sown common vetch and hairy vetch,which increased N transportation quantity and transportation rate at pre-anthesis,enhanced N harvest index,optimized N nutrition index,and increased activities of nitrate reductase and glutamine synthetase of leaf,respectively.Meanwhile,mixed sown common vetch and hairy vetch under reduced N by 20%improved soil organic matter and N contents.Therefore,mixed sown common vetch and hairy vetch can substitute 40%of chemical N fertilizer while maintaining grain yield and N accumulation,and it combined with reduced chemical N by 20%or 40%improved NUE of wheat via enhancing N supply and uptake.

Legume Green Manure and Intercropping for High Biomass Sorghum Production

Before the advent of cheap, synthetic fertilizers, legumes were commonly used as green manure crops for their ability to fix atmospheric nitrogen (N). A three-year study at Overton, TX examined legume integration into high-biomass sorghum (Sorghum bicolor L.) production systems on a Lilbert loamy fine sand recently cultivated after a fertilized bermudagrass [Cynodon dactylon (L.) Pers.] pasture. In this split-split plot design, ‘Dixie’ crimson clover (Trifolium incarnatum L.) and ‘Iron and Clay’ cowpea (Vigna unguiculata L.) were integrated into a high-biomass sorghum production system to evaluate impacts on N concentration, C concentration, and yield of high-biomass sorghum and their impacts on soil total N and soil organic carbon (SOC). Main plots were split into crimson clover green manure (CLGM) and winter fallow (FALL) followed by three sub-plots split into warm-season crop rotations: cowpea green manure (CPGM), cowpea-sorghum intercrop (CPSR), and sorghum monocrop (SORG). Three N fertilizer treatments (0, 45, 90 kg N∙ha−1) were randomized and applied as sub-sub plots. The CLGM increased (P sorghum biomass yield (16.5 t DM∙ha−1) 28% in year three but had no effect in the first two years. The CPSR treatment reduced sorghum yield up to 62% compared to SORG;whereas CPGM increased sorghum yield 56% and 18% the two years following cowpea incorporation. Rate of N fertilizer had no effect on sorghum biomass yield. Decrease in SOC and soil N over time indicated mineralization of organic N and may explain why no N fertilizer response was observed in sorghum biomass yield. Cowpea showed strong potential as a green manure crop but proved to be too competitive for successful intercropping in high-biomass sorghum production systems.

A Biogas Production Model from the Combination of Pig Manure and Cow Dung in N’Zérékoré City, Republic of Guine

This present research work focuses on the valorization of pig droppings for production of biogas in mono digestion and co-digestion with proportions of cow dung from the urban commune of N’Zérékoré. It was carried out in December 2020 in the Physics laboratory of the University of N’Zérékoré. The anaerobic digestion process took 25 days in an almost constant ambient temperature of 25˚C. Five digesters were loaded on 12/06/2020, two of which with 1 kg of pig dung and 1 kg of cow dung both in mono-digestion. The 3 other digesters in co-digestion with different proportions of pig manure and cow dung. The substrate in each digester is diluted in 2 liters of water, with a proportion of (1/2). The main results obtained are: 1) the evolution of the temperature and pH during digestion process, 2) the average biogas productions 0.61 liters for (D1);1.20 liter for (D2);1.65 liter for (D3);1.51 liter for (D4) and 1.31 liter for (D5). The cumulative amounts of biogas are respectively: D1 (7.95 liters), D2 (15.60 liters), D3 (21.50 liters), D4 (19.65 liters) and D5 (17.05 liters). The total cumulative production is 81.75 liters at the end of the process. The originality of this research work is that the proposed model examines the relation between the daily biogas production and the variation of temperature, pH and pressure. The combustibility test showed the biogas produced during the first week was no combustible (contains less than 50% methane). Combustion started from the biogas produced from the 15th day and it is from the 20th day that a significant amount of stable yellow/blue flame was observed. The results of this study show the combination of pig manure and cow dung presents advantages for optimal biogas production.

Impact on Soil Organic C and Total Soil N from Cool- and Warm-Season Legumes Used in a Green Manure-Forage Cropping System

Annual forage legumes are important components of livestock production systems in East Texas and the southeastern US. Forage legumes contribute nitrogen (N) to cropping systems through biological N fixation, and their seasonal biomass production can be managed to complement forage grasses. Our research objectives were to evaluate both warm- and cool-season annual forage legumes as green manure for biomass, N content, ability to enhance soil organic carbon (SOC) and soil N, and impact on post season forage grass crops. Nine warm-season forage legumes (WSL) were spring planted and incorporated as green manure in the fall. Forage rye (Secale cereale L.) was planted following the incorporation of WSL treatments. Eight cool-season forage legumes (CSL) were fall planted in previously fallow plots and incorporated as green manure in late spring. Sorghum-sudangrass (Sorghum bicolor x Sorghum bicolor var. sudanense) was planted over all treatments in early summer after forage rye harvest and incorporation of CSL treatments. Sorghum-sudangrass was harvested in June, August and September, and treatments were evaluated for dry matter and N concentration. Soil cores were taken from each plot, split into depths of 0 to 15, 15 to 30 and 30 to 60 cm, and soil C and N were measured using combustion analysis. Nylon mesh bags containing plant samples were buried at 15 cm and used to evaluate decomposition rate of above ground legume biomass, including change in C and N concentrations. Mungbean (Vigna radiata L. [Wilczek]) had the highest shoot biomass yield (6.24 t DM ha-1) and contributed the most total N (167 kg∙ha-1) and total C (3043 kg∙ha-1) of the WSL tested. Decomposition rate of WSL biomass was rapid in the first 10 weeks and very slow afterward. Winter pea (Pisum sativum L. spp. sativum), arrow leaf clover (Trifolium vesiculosum Savi.), and crimson clover (Trifolium incarnatum L.) were the most productive CSL in this trial. Austrian winter pea produced 8.41 t DM ha-1 with a total N yield of 319 kg N ha-1 and total C production of 3835 kg C ha-1. The WSL treatments had only small effects on rye forage yield and N concentration, possibly due to mineralization of N from a large SOC pool already in place. The CSL treatments also had only minimal effects on sorghum-sudangrass forage production. Winter pea, arrow leaf and crimson clover were productive cool season legumes and could be useful as green manure crops. Mungbean and cowpea (Vigna unguiculata [L.] Walp.) were highly productive warm season legumes but may include more production risk in green manure systems due to soil moisture competition.

Sterilization Effects of Bacterial Inhibitor on Escherichia coli in Cattle Manure Compost

[Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor(CaCN2)during the process of cattle manure composting so as to provide a theoretical basis for cattle manure harmless treatment.[Method] Both experimental groups supplemented with 2.0% bacterial inhibitor and control groups without bacterial inhibitor were cultured under different temperatures(20,30,37,50,60 ℃)to determine the optimal composing temperature.Under 30 ℃,different bacterial inhibitor doses(0,2.0%,2.5%,3.0%)were added into the compost to obtain the optimal bacterial inhibitor addition dose.[Result] 30,50 and 60 ℃ were ideal temperatures for sterilization of E.coli.Under 30 ℃,E.coli couldn't be detected in 2.5% dose group and 3.0% dose group after culture for 48 h,demonstrating no less than 2.5% bacterial inhibitor should be added.[Conclusion] It has an important significance to enhance the sterilization effects on E.coli by adding CaCN2 into cattle manure compost especially in winter.

应用Manure-DNDC模型模拟畜禽养殖氮素污染

畜禽养殖是重要的农业面源氮素污染源头,大量的畜禽粪便施入农田后,会加大农田氮素径流和淋溶损失强度。畜禽养殖废弃物氮素污染过程复杂,涉及到动物自身营养循环以及废弃物通过不同途径进入环境的过程,目前大多通过排放系数法估算畜禽养殖过程产生的氮素污染负荷。该文选用最新版Manure-DNDC模型,以山东小清河流域为例,模拟畜禽养殖及废弃物处理的生物地球化学过程,分析氮素在动物、畜禽粪便、农田之间的迁移转化,探讨该过程中氮素的主要损失途径以及污染物负荷的时空变化特征。模拟结果表明,小清河流域2008年畜禽养殖及粪便处理场所氮素径流损失4.66万t,粪便施入农田后的氮素径流和淋溶损失分别为0.1、0.51万t。

Research Progress on Resource Utilization of Livestock and Poultry Manure

Although mass livestock and poultry breeding brought magnificent econom-ic benefits to the society, it made great harm to the environment. By reviewing the current status of environmental pol ution caused by livestock and poultry manure in China, harm-free treatment technology for livestock and poultry manure including fer-tilization technology, feed processing technology and energy technology were elabo-rated so as to address the existing issues in this regard and provide references for resource utilization and eliminating environmental pol ution through modified technical system and environment laws.

Study on Compound Substrate Properties with Spent Mushroom Compost and Cattle Manure Compost and Effects on the Growth of Seedlings

[Objective] The purpose was to study the optimum composition ratio of compound substrate with spent mushroom compound （SMC） and cattle manure com- post （CMC） for the seedling growth of tomato, cucumber and watermelon. [Method] With internationally best formula substrate （turf：vermiculite=2：1） used for CK, SMC and CMC were matched according to different proportions to get different substrate whose physical and chemical nutrient properties and their effects on the growth of tomato, cucumber and watermelon were studied by means of plug seeding technolo- gy. [Result] The results showed that the bulk density, porosity and the pH of the compound substrates are all in the ideal condition. However, CMC increased the EC value and the pH of the compound substrates. Compound substrates with high ratio of CMC are not suitable for seedlings. [Conclusion] Tomato and watermelon seedlings grew well in the compost substrate with SMC：CMC=3：1 with no river sand. And the cucumber seedlings grew well in the compost substrate with SMC：CMC=2：1 with 5% volume river sand.

Effects of Green Manure Rotation on Rice Growth Dynamics and Nitrogen Uptake and Utilization

This study aimed to comprehensive evaluation of different winter green manure on characterization of nitrogen uptake and utilization, to provide the basis for N fertilizer reasonable operation and characteristics improvement of nitrogen nutrition in rice high-yield cultivation. This experiment was set to compare milk vetch, rapeseed,ryegrass and mixed of green manure on rice yield, rice growth dynamics and nitrogen uptake and utilization in rice. The results showed that among 4 different winter green manure, the treatment of MV-R-R（milk vetch-rice-rice） for the early rice yield was the most than others treatments. Compared with RG-R-R（ryegrass-rice-rice）, RPR-R（rapeseed-rice-rice）, MS-R-R（mixed green manure-rice-rice）, the treatment of MVR-R inceased by 6.61%, 3.29%, 0.78%, respectively. The treatment of MV-R-R in N content in plant of rice was maximized in the tillering, booting, heading and maturity periods, respectively higher than the average of other treatments 9.68%, 19.72%,6.23% and 8.66%. At tillering, booting, heading and maturity, the treatment of MV-R-R were the highest in N uptake, RP-R-R minimum. The N periodic accumulation for MV-R-R were higher than other treatments in the tillering to booting, booting to heading and heading to maturity periods. The rates respectively were 21.81%, 68.73% and286.5%. In addition, N periodic accumulation and its ratio to total in the heading to maturity was minimum, maximum before tillering under green manure rotation system.So the cropping system of milk vetch-rice-rice could increase nitrogen use efficiency and improve N cycling.

Effects of Feed Fermentation on Chemical Fractionation of Trace Elements in Feed and Pig Manure and Skatole Content in Pig Manure

[Objective] This study aimed to promote the combination of cultivation and livestock farming, and to explore an environment-protecting farming style. [Method]The effects of anaerobically fermented complete compound sow feed, added with Lactobacillus and Bacillus subtilis, on the chemical fractionation of copper, iron, zinc and manganese in feed and pig manure and skatole content in pig manure were investigated. [Result] Compared with those in the non-fermented feed, in the fermented feed and pig manure, the acetic acid-extractable copper, iron, zinc and manganese contents increased significantly（P0.05）, the reduced copper and iron contents increased significantly（P 0.05）, the oxidized copper and iron contents reduced significantly（P0.05）, and the residual copper contents remained unchangeable（P0.05）. The pH value of fermented feed decreased significantly（P0.05）, and that of pig manure increased significantly（P0.05）. The skatole content in pig manure decreased significantly（P 0.01）. The Lactobacillus abundance and amylase and cellulase activity increased significantly（P 0.05）. [Conclusion] The fermentation of feed changed the chemical fractionation of copper, iron, zinc and manganese in feed and pig manure, and reduced the manure odor.

Isolation,Screening and Identification of High-temperature Cellulolytic Microbes in Pig Manure

[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.

Effects of Bacteria and Accessories on Chicken Manure Compost

[Objective] In order to study the influence of bacteria and accessories of chicken manure compost.[Method] The experiment was conducted to investigate the changes of temperature,the quantification of Escherichia coli and the odor during chicken manure compost by adding sawdust,rice husk,yeast and lactic acid bacteria respectively.[Result] The results showed that：（1） The yeast group reached the highest fermentation temperature 67.6 ℃,which was 6.9 ℃ higher than that of lactic acid bacteria group,and the fermentation time of yeast group kept over 55 ℃for 16 days,which was 5 days longer than that of lactic acid bacteria group,both of which were better than that of control group（55.9 ℃,5 days）;（2）The highest fermentation temperature of sawdust group was 2.2 ℃ higher than that of rice husk group,and the fermentation time of sawdust group kept over 55 ℃ was 3 days longer than that of rice husk group;（3） The quantification of E.coli reduced from10^5 to 10^2per gram in both of the yeast group and the lactic acid bacteria group.The odor of the yeast group and the lactic acid bacteria group disappeared in the seventh day and the eighth day respectively.[Conclusion] The results showed that the adding of yeast and sawdust was the best condition for chicken manure composting in this experiment.

Effects of Applying Bio-bacterial Fertilizer on Aroma Components of Flue-cured Tobacco

Objective] This research aimed to investigate the effects of bio-bacterial fertilizer on quality of flue-curved tobacco. [Method] The various concentrations of bio-bacterial fertilizer were applied to flue-cured tobacco as leaf fertilizer and base fertilizer respectively. [Result] The effect of bio-bacterial fertilizer on flue-cured tobac-co differed among different treatments. Compared with the control, the bio-bacterial fertilizer significantly affected the quality of flue-cured tobacco. Among al the treat-ments, Treatment 3（leaf fertilizer） showed the best treatment effect, and it significant-ly improved the quality of flue-cured tobacco. [Conclusion] The bio-bacterial fertilizer showed significant improving effect on quality of flue-cured tobacco if spayed as leaf fertilizer.

Study on Environmental and Economic Benefits of Rice and Wheat Rotation with Swine Manure-Straw Returning

[Objective] The aim was to investigate the application effect of swine manure-straw returning and to determine the best mode. [Method] A field experiment under rice and wheat rotation with different swine manure-straw treatments was con- ducted to study the growth characters and output of rice and wheat, calculate the economic benefit and carbon dioxide emission reduction, and analyze the best mode of swine manure applying-straw returning. [Result] The swine manure-straw returning was conducive to the growth of crop, the highest outputs of rice and wheat were on the treatment of ＂30% swine manure and 20% straw and 50% chemical fertiliz- er＂, they were 7 874.57 and 6 427.00 kg/hm^2, and saved cost about 5 146.35 Yuan/hm^2, increased input 5 312.56 and 3 931.93 Yuan/hm^2, the greenhouse gas e- mission reduction was 1.30 t/hm^2 （calculated according to carbon dioxide on a dry basis）. [Conclusion] The treatment of ＂30% swine manure and 20% straw and 50% chemical fertilizer＂ was the best mode of swine manure-straw returning.