Comparative study of chitosan/fibroin–hydroxyapatite and collagen membranes for guided bone regeneration in rat calvarial defects: micro-computed tomography analysis

This study aimed to utilize micro-computed tomography （micro-CT） analysis to compare new bone formation in rat calvarial defects using chitosan/fibroin-hydroxyapatite （CFB-HAP） or collagen （Bio-Gide） membranes. Fifty-four （54） rats were studied. A circular bony defect （8 mm diameter） was formed in the centre of the calvaria using a trephine bur. The CFB-HAP membrane was prepared by thermally induced phase separation. In the experimental group （n= 18）, the CFB-HAP membrane was used to cover the bony defect, and in the control group （n= 18）, a resorbable collagen membrane （Bio-Gide） was used. In the negative control group （n= 18）, no membrane was used. In each group, six animals were euthanized at 2, 4 and 8 weeks after surgery. The specimens were then analysed using micro-CT. There were significant differences in bone volume （BV） and bone mineral density （BMD） （P〈O.05） between the negative control group and the membrane groups. However, there were no significant differences between the CFB-HAP group and the collagen group. We concluded that the CFB-HAP membrane has significant potential as a guided bone regeneration （GBR） membrane.

Real-time-guided bone regeneration around standardized critical size calvarial defects using bone marrow-derived mesenchymal stem cells and collagen membrane with and without using tricalcium phosphate: an in vivo microcomputed tomographic and histologic e

The aim of the present real time in vivo micro-computed tomography （pCT） and histologic experiment was to assess the efficacy of guided bone regeneration （GBR） around standardized calvarial critical size defects （CSD） using bone marrow-derived mesenchymal stem cells （BMSCs）, and collagen membrane （CM） with and without tricalcium phosphate （TCP） graft material. In the calvaria of nine female Sprague-Dawley rats, full-thickness CSD （diameter 4.6 mm） were created under general anesthesia. Treatment-wise, rats were divided into three groups. In group 1, CSD was covered with a resorbable CM; in group 2, BMSCs were filled in CSD and covered with CM; and in group 3, TCP soaked in BMSCs was placed in CSD and covered with CM. All defects were closed using resorbable sutures. Bone volume and bone mineral density of newly formed bone （NFB） and remaining TCP particles and rate of new bone formation was determined at baseline, 2, 4, 6, and 10 weeks using in vivo pCT. At the lOth week, the rats were killed and calvarial segments were assessed histologically. The results showed that the hardness of NFB was similar to that of the native bone in groups I and 2 as compared to the NFB in group 3. Likewise, values for the modulus of elasticity were also significantly higher in group 3 compared to groups 1 and 2. This suggests that TCP when used in combination with BMSCs and without CM was unable to form bone of significant strength that could possibly provide mechanical ＂lock＂ between the natural bone and NFB. The use of BMSCs as adjuncts to conventional GBR initiated new bone formation as early as 2 weeks of treatment compared to when GBR is attempted without adiunct BMSC therapy.

Experimental study of exogenous collagen membrane in myringoplasty: a preliminary report

Myringoplasty using exogenous collagen membrane was performed in 20 earsof 10 rabbits after complete removal of drumhead.Healing results were observed at 10,20,40and 60d after operation.Nineteen ears were transplanted successfully.Meanwhile,thehistology and ultrastructural morphology of the rabbit tympanic membranes were com-pared before and after transplantation.The results confirmed that the graft has a goodproperty of biocompatibility,and that it is useful in tympanic membrane transplantation.

Collagen/Chitosan Electrospun Nanofibrous Membrane for Wound Dressing

Collagen(Col)/chitosan(CS)nanofibrous membrane has great potential to be used as wound dressing.However,current Col/CS nanofibrous membrane produced from electrospinning can not offer sufficient mechanical strength for practical applications.Herein,a novel mixed solvent was used to prepare next-generation high-strength Col/CS nanofibrous membrane.Meanwhile,the optimal Col to CS weight ratio was investigated as well.The asproduced membrane was examined by scanning electron microscopy(SEM),attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR),differential scanning calorimetry(DSC),and XF-1A tester to study its morphological,chemical,thermal and mechanical properties.The preliminary results demonstrated that the mechanical properties of Col/CS nanofibrous membranes were enhanced substantially with the increase of CS weight ratios from 0 to 90%and the optimal Col to CS weight ratio was determined to be 1∶1.A promising way was presented to fabricate Col/CS electrospun nanofibrous membrane with sufficient mechanical strength for practical wound dressing applications.

联合应用Bio-oss骨代材料和Bio-gide胶原膜行引导骨组织再生术治疗重度牙周炎的效果

目的 评估重度牙周炎在引导骨组织再生术中Bio-gide+Bio-oss的作用。方法 方便选取2020年12月—2021年12月上海市第六人民医院金山分院的74例重度牙周炎患者,随机分为常规组和再生组,每组37例,常规组行翻瓣术治疗,再生组行Bio-oss骨代材料+Bio-gide胶原膜引导骨组织再生术,对比两组有效率、龈沟液内炎症因子、牙周指数、牙周疼痛感及并发症。结果 再生组牙周炎的治疗有效率为97.30%,高于常规组(78.38%),差异有统计学意义(χ^(2)=4.554,P<0.05)。术后再生组龈沟液中的炎症情况、牙周指数均比常规组低,差异有统计学意义(P<0.05)。再生组轻度疼痛率(89.19%)比常规组(59.46%)高,中度疼痛率(10.81%)比常规组(35.14%)低,差异有统计学意义(χ^(2)=8.568、6.186,P<0.05)。再生组牙周炎治疗的并发症发生率2.70%,比常规组(21.62%)低,差异有统计学意义(χ^(2)=4.554,P<0.05)。结论 Bio-gide+Bio-oss使用后达到的骨组织再生效果好,能纠正牙周指数,缓解牙周炎症,且牙周创伤小。

Comparative evaluation of effect of injectable platelet-rich fibrin with collagen membrane compared with collagen membrane alone for gingival recession coverage

BACKGROUND Collagen membrane and platelet-rich fibrin(PRF)have emerged as vital biomaterials in the field of periodontal regeneration.Minimally invasive techniques are being preferred by most periodontists,as it is patient compliant with fewer post-surgical complications as compared to conventional surgical techniques.Thus,in this study we have evaluated the effect of injectable PRF(i-PRF)with collagen membrane compared with collagen membrane alone using vestibular incision subperiosteal tunnel access(VISTA)technique for gingival recession coverage.AIM To compare the efficacy of VISTA using collagen membrane with collagen membrane soaked in injectable PRF for gingival recession coverage.METHODS A split mouth randomized controlled clinical trial was designed;13 subjects having at least 2 teeth indicated for recession coverage were enrolled in this study.The sites were randomly assigned to control group(VISTA using collagen membrane alone)and the test group(VISTA using collagen membrane with i-PRF).The clinical parameters assessed were pocket depth,recession depth(RD),recession width(RW),relative attachment level,keratinised tissue width(KTW),keratinised tissue thickness(KTT),and percentage root coverage.RESULTS RD showed a statistically significant difference between the test group at 3 mo(0.5±0.513)and 6 mo(0.9±0.641)and the control group at 3 mo(0.95±0.51)and 6 mo(1.5±0.571),with P values of 0.008 and 0.04,respectively.RW also showed a statistically significant difference between the test group at 3 mo(1±1.026)and 6 mo(1.65±1.04)and the control group at 3 mo(1.85±0.875)and 6 mo(2.25±0.759),with P values of 0.008 and 0.001,respectively.Results for KTW showed statistically significant results between the test group at 1 mo(2.85±0.489),3 mo(3.5±0.513),and 6 mo(3.4±0.598)and the control group at 1 mo(2.45±0.605),3 mo(2.9±0.447),and 6 mo(2.75±0.444),with P values of 0.04,0.004,and 0.003,respectively.Results for KTT also showed statistically significant results between test group at 1 mo(2.69±0.233),3 mo(2.53±0.212),and 6 mo(2.46±0.252)and the control group at 1 mo(2.12±0.193),3 mo(2.02±0.18),and 6 mo(1.91±0.166),with P values of 0.001,0.001,and 0.001,respectively.The test group showed 91.6%,81.6%,and 67%root coverage at 1 mo,3 mo,and 6 mo,while the control group showed 82.3%,66.4%,and 53.95%of root coverage at 1 mo,3 mo,and 6 mo,respectively.CONCLUSION The use of minimally invasive VISTA technique along with collagen membrane and injectable form of platelet-rich fibrin can be successfully used as a treatment method for multiple or isolated gingival recessions of Miller’s class-I and class-II defects.

Bio-oss结合Bio-gide修复牙种植体周围骨缺损的组织学研究

目的:通过制作带有种植体的硬组织磨片,评价无机牛骨(deproteinizednaturalbovinemineral,Bio-oss)结 合可吸收性胶原膜(bioresorbablecollagenmambrane,Bio-gide)在牙种植体周围骨缺损中的引导骨再生作用及效 果。方法:在兔股骨植入羟基磷灰石涂层BLB种植体,并在其外侧壁制造标准骨缺损,A组在骨缺损处植入Bio- oss颗粒并在其表面覆盖Bio-gide膜,B组作为空白对照,分别于术后1、2、4、6个月取样品,通过带种植体的硬组 织切片进行骨组织形态学分析。结果:术后1个月Bio-oss颗粒表面有新骨形成,随时间延长Bio-oss发生降解 吸收,新生骨量增加,并与种植体表面形成骨性结合。结论:可吸收性胶原膜Bio-gide结合Bio-oss应用于牙种 植体周围骨缺损中,可以引导骨组织再生,重建缺损的骨组织,新生骨与种植体形成骨性结合。

采用Bio-Oss胶原骨联合Bio-Gide胶原屏障膜行引导骨再生术修复牙龈瘤电切术后牙槽骨缺损的临床效果

目的探讨采用Bio-Oss胶原骨联合Bio-Gide胶原屏障膜行引导骨再生术修复牙龈瘤电切术后牙槽骨缺损的临床效果。方法将64例存在牙槽骨缺损的牙龈瘤患者随机分为研究组和对照组,各32例,所有患者高频牙龈电凝刀切除牙龈瘤后均保留累及牙齿。研究组创口植入Bio-Oss胶原骨并覆盖Bio-Gide胶原屏障膜,对照组创口覆盖牙周塞制剂。术后随访1年,比较两组保留牙齿的牙髓敏感度(PS)、牙松动度、牙周附着丧失(PAL)情况,并采用X线片观察牙槽骨变化。结果牙龈瘤电切术中得以保留的研究组47枚牙龈瘤术前累及牙齿和对照组42枚牙龈瘤术前累及牙齿,术毕均有不同程度牙槽骨缺损,但无松动。64例牙龈瘤患者术后1年均无复发。术后1、3、6、12个月,研究组保留牙齿的PS、牙松动度和PAL改善情况均优于对照组(均P<0.05),研究组保留牙齿的PS、牙松动度、PAL改善情况随着时间的推移无明显变化,而对照组保留牙齿的PS、牙松动度、PAL改善情况随着时间的推移持续加重。X线片可见研究组牙槽骨稳定无吸收,对照组牙槽骨呈进展性吸收。结论Bio-Oss胶原骨和Bio-Gide胶原屏障膜联用行引导骨再生术,能有效地修复牙龈瘤电切术后的牙槽骨缺损,改善牙周状况,对牙周、牙髓病变起到积极的预防作用。

人骨髓间充质干细胞体外培养及与Bio-gide胶原膜复合培养的实验研究

目的本实验拟研究人骨髓间充质干细胞(human mesenchymal stemcells,hMSCs)分离、培养与鉴定的方法,探讨其作为牙周组织工程种子细胞的可行性。方法体外分离培养hMSCs,进行形态学观察,细胞表面抗原检测;将hMSCs与Bio-gide胶原膜进行体外复合培养,通过倒置相差显微镜,扫描电镜进行观察。结果梯度密度离心法结合贴壁筛选法能获得高纯度高活性的hMSCs。hMSCs可在Bio-gide胶原膜上贴附增殖,并复层生长。结论hMSCs取材方便、创伤小;扩增分化能力强。hMSCs可作为牙周组织工程种子细胞来源。Bio-gide胶原膜可作为细胞生长载体。

Bio-oss骨代材料与Bio-gide膜治疗重度牙周病疗效评估

目的观察联合应用Bio-oss骨代材料和Bio-gide胶原膜行引导骨组织再生术治疗重度牙周炎的疗效。方法将46例重度牙周病患者随机分为实验组和对照组,各23例,实验组39颗患牙行Bio-oss、Bio-gide引导性组织再生术,对照组32颗患牙行常规翻瓣术刮治。分别记录术前,术后12个月患牙周牙探诊深度(periodontal probing depth,PPD)、附着丧失(attachment loss,AL)、龈沟出血指数(sulcus bleeding index,SBI)、牙松动度(teeth mobility,TM)及牙槽骨高度(alveolar bone height,AH)。结果与术前相比两组术后PPD、AL、SBI、TM均明显减少,且有显著性差异,实验组疗效优于对照组。X线根尖片显示,实验组骨缺损区有新骨形成,AH增加;对照组未见新骨形成,AH无明显改善。而且实验组骨密度增强较对照组明显增多(P<0.05)。结论牙周翻瓣术后植入Bio-oss骨代材料,联合应用Bio-gide可吸收胶原膜治疗重度牙周病能明显改善临床指标,且疗效优于单纯翻瓣刮治术。

Bio-gide胶原膜体外复合培养犬骨髓间充质干细胞的软骨分化

背景:应用组织工程方法修复股骨头坏死后的软骨损伤应解决的2个关键因素是种子细胞和支架材料。目的:探讨Bio-gide胶原膜体外与犬骨髓间充质干细胞复合成软骨的可行性。方法:采用全骨髓血离心法结合贴壁筛选法体外分离培养比格犬骨髓间充质干细胞,观察细胞形态学变化,以细胞表面抗原进行鉴定。采用成软骨诱导培养基诱导第3代骨髓间充质干细胞向软骨细胞分化作为实验组,设置普通培养基培养对照组,MTT比色法测试软骨细胞生长曲线,并行Ⅱ型胶原免疫组织化学和甲苯胺蓝染色等生物学鉴定。将第3代骨髓间充质干细胞与Bio-gide胶原膜体外复合培养行倒置相差显微镜、扫描电镜观察。结果与结论:采用全骨髓血离心法结合贴壁筛选法获得了高纯度高活性的骨髓间充质干细胞,生长状态良好,扩增能力强,并成功诱导分化为软骨细胞。大量骨髓间充质干细胞在Bio-gide胶原膜上贴附增殖并复层生长,细胞与Bio-gide胶原膜逐渐融为一体;在胶原膜的断面可见胞体伸出突起相互连接包绕胶原膜,其间有明显细胞基质分泌。表明犬骨髓间充质干细胞可在Bio-gide胶原膜上生长并向软骨细胞分化。

Bio-Gide胶原膜结合Bio-Oss多孔骨治疗牙周垂直型骨缺损的6个月临床评价

目的 评价Bio Gide胶原膜结合Bio Oss多孔骨治疗牙周垂直型骨缺损的临床疗效。方法 15例慢性牙周炎患者经过基础治疗后口腔内仍存在 2个探诊深度 6mm以上的骨下袋 ,随机选出 1个骨缺损用Bio Gide胶原膜结合Bio Oss多孔骨修复 ,另一个骨缺损仅用Bio Gide胶原膜进行修复治疗 ,分别于手术前及手术后 6个月测量各项临床指标。结果 术后两组各项临床指标均有明显改善 ,且具有统计学意义 (P <0 .0 0 1)。牙周袋探诊深度、临床附着水平丧失、骨水平的变化在两组间无显著性差异 ,边缘龈退缩在单独应用Bio Gide胶原膜组更加明显(P <0 .0 5 )。结论 尚不能肯定Bio Oss多孔骨可以加强Bio Gide胶原膜修复牙周垂直型骨缺损的临床效果。

Bio-oss胶原质及Bio-gide胶原膜治疗牙周病的临床研究

目的:观察联合应用Bio-oss胶原质和Bio-gide胶原膜行引导骨组织再生术治疗牙周炎的疗效。方法:将42例牙周病患者随机分为实验组和对照组各21例,实验组35颗患牙行Bio-oss、Bio-gide引导性组织再生术,对照组30颗患牙行常规翻瓣术刮治。分别记录术前、术后12个月患牙周袋深度(periodontal probing depth,PPD)、附着丧失(attachment loss,AL)、牙松动度(teeth mobility,TM)。结果:与术前相比,2组术后PPD、AL、TM均明显减少,且有显著性差异,实验组疗效优于对照组。X线根尖片显示,实验组骨缺损区有新骨形成,对照组未见新骨形成。而且实验组骨密度增强较对照组明显增多。结论:牙周翻瓣术后植入Bio-oss骨代材料,联合应用Bio-gide胶原膜治疗牙周病能明显改善临床指标,且疗效优于单纯翻瓣刮治术。

High matrix metalloproteinase-9 expression induces angiogenesis and basement membrane degradation in stroke-prone spontaneously hypertensive rats after cerebral infarction

Basement membrane degradation and blood-brain barrier damage appear after cerebral infarc- tion, severely impacting neuronal and brain functioning; however, the underlying pathogenetic mechanisms remain poorly understood. In this study, we induced cerebral infarction in stroke- prone spontaneously hypertensive rats by intragastric administration of high-sodium water （1.3% NaC1） for 7 consecutive weeks. Immunohistochemical and immunofluorescence assays demonstrated that, compared with the non-infarcted contralateral hemisphere, stroke-prone spontaneously hypertensive rats on normal sodium intake and Wistar-Kyoto rats, matrix metalloproteinase-9 expression, the number of blood vessels with discontinuous collagen IV expression and microvessel density were significantly higher, and the number of continuous collagen IV-positive blood vessels was lower in the infarct border zones of stroke-prone sponta- neously hypertensive rats given high-sodium water. Linear correlation analysis showed matrix metalloproteinase-9 expression was positively correlated with the number of discontinuously collagen IV-labeled blood vessels and microvessel density in cerebral infarcts of stroke-prone spontaneously hypertensive rats. These results suggest that matrix metalloproteinase-9 upregula- tion is associated with increased regional angiogenesis and degradation of collagen IV, the major component of the basal lamina, in stroke-prone spontaneously hypertensive rats with high-sodi- um water-induced focal cerebral infarction.

拔牙窝内即刻植入Bio-Oss颗粒联合Bio-Gide胶原膜表面覆盖在磨牙区拔牙中的应用

目的:探究拔牙窝内即刻植入Bio-Oss颗粒联合Bio-Gide胶原膜表面覆盖在磨牙区拔牙中的应用效果。方法:选取2016年1月~2018年6月我院60例磨牙区拔牙患者,依据随机数字表法分组,各30例。两组均行磨牙区拔牙,对照组拔牙后行常规处理,观察组拔牙后即刻于拔牙窝内植入Bio-Oss颗粒,并于表面覆盖Bio-Gide胶原膜。统计对比两组临床效果、新骨轮廓及骨密度评分,并对比拔牙前、拔牙6个月后牙槽骨宽度与高度。结果:两组拔牙后均无并发症发生,拔牙伤口愈合良好。1个月,患者牙龈基本愈合,拔牙伤口关闭;3~6个月,对照组颊舌侧牙龈愈合,但牙槽骨外形较拔牙前缩窄,牙槽骨宽、高度则减少,特别是颊侧凹陷显著;观察组胶原膜被牙龈覆盖,颊舌侧牙龈呈平台状愈合,牙槽骨外形丰满;观察组新骨轮廓、骨密度评分较对照组高(P<0.05);两组拔牙后6个月牙槽骨宽度、高度均较拔牙前有所下降,但观察组高于对照组(P<0.05)。结论:磨牙区拔牙患者拔牙后于拔牙窝内即刻植入Bio-Oss颗粒联合Bio-Gide胶原膜表面覆盖,可显著减少因拔牙造成的牙槽骨吸收,维持拔牙位点骨量,为后期种植牙修复提供良好位点条件,且安全性高。

Porous titanium granules in critical size defects of rabbit tibia with or without membranes

Recently, porous titanium granules （PTGs） have been indicated for the preservation of the dimensions of post-extraction sockets, as a filler in sinus lift procedures and for the treatment of peri-implant and periodontal defects, based on the osteoconductivity and dimensional stability of the titanium granules. However, there is a lack of information regarding the use of this material in larger defects and in conjunction with membranes. The objective of this study is to test the behavior of PTGs used to fill critical size defects in rabbit tibiae, with and without membranes. Critical defects were created in both tibiae of rabbits, divided randomly into three groups： Group A （defect filled with PTG）, Group B （defect filled with PTG＋collagen membrane） and a control group （empty defect）. After six weeks, histomorphometric analysis was performed. The results showed more defect closures at the cortical area （87.37%±2.2%） and more bone formation at the marrow area （57.6%± 1.3%） in Group B, in comparison with the other groups （P〈0.05）; the use of membranes improved the material stability expressed as more percentages of the original material when membranes were used （P〈0.05）. Finally, inflammatory reactions were observed when the granules were not protected by membranes. In spite of the limitations of this animal study, it may be concluded that PTG particles are osteoconductive and allow bone growth. The PTG particles must be covered by a membrane, especially when grafting larger defects, in order to control particle migration, promote clot stabilization and separate the PTG graft from undesired soft tissue cells.

沙丁鱼鱼鳞胶原蛋白肽的制备及其抗氧化活性研究

目的优化沙丁鱼(Sardinapilchardus)鱼鳞胶原蛋白肽的制备工艺和评价不同分子量大小胶原蛋白肽的抗氧化活性。方法以沙丁鱼鱼鳞为原料,通过酶工程结合单因素与响应面实验优化胶原蛋白肽制备工艺,采用超滤膜分级分离鱼鳞胶原蛋白肽,并通过体外自由基化学体系分析对比不同分子量大小(10、5、1 kDa)的胶原蛋白肽的抗氧化活性。结果采用碱性蛋白酶进行酶解鱼鳞,胶原蛋白肽的得率最高;最优酶解工艺参数为:碱性蛋白酶加酶量7526 U/g、料液比1:20(g/mL)、pH 10.5、酶解温度67℃、酶解时间4 h,此条件下鱼鳞胶原蛋白肽得率最高,达43.44%±1.59%。通过超滤获得的分子量小于1k Da的鱼鳞胶原蛋白肽对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和羟基自由基(hydroxyl radical,·OH)的清除能力最强,其半抑制浓度(half maximal inhibitory concentration,IC_(50))分别为0.72 mg/mL和1.76 mg/mL;分子量为1~5 kDa的鱼鳞胶原蛋白肽对超氧阴离子自由基(superoxide radical,O^(2-)·)的清除效果最好,其IC50为1.45 mg/mL。结论碱性蛋白酶能够有效制备鱼鳞胶原蛋白肽,且小于1 kDa的鱼鳞胶原蛋白肽抗氧化活性最好。

载骨髓间充质干细胞的高孔聚己内酯-胶原纳米纤维膜修复大鼠长期鼓膜穿孔

背景:近年来出现许多新颖的鼓膜修补材料,包括贴片、3D打印支架等,然而经过这些材料修补后的鼓膜在厚度和内部结构上不同于天然鼓膜。目的:探究载骨髓间充质干细胞的高孔聚己内酯-胶原纳米纤维膜修补大鼠长期鼓膜穿孔的效果。方法:利用静电纺丝技术分别制备聚己内酯、聚己内酯-胶原与高孔聚己内酯-胶原纳米纤维膜,表征支架的表面形态、孔隙率及细胞相容性。取50只雄性SD大鼠,使用无菌23号针头刺破双耳鼓膜后下部联合丝裂霉素C、氢化可的松用药法建立鼓膜穿孔模型;造模12周后,采用随机数字表法将大鼠分为5组:空白对照组不进行任何治疗,其他4组鼓膜穿孔处分别植入聚己内酯纳米纤维膜(聚己内酯组)、聚己内酯-胶原纳米纤维膜(聚己内酯-胶原组)、高孔聚己内酯-胶原纳米纤维膜(高孔聚己内酯-胶原组)与载骨髓间充质干细胞的高孔聚己内酯-胶原纳米纤维膜(载细胞高孔聚己内酯-胶原组),每组10只,支架植入1,2,3,4周利用耳内镜检查鼓膜愈合情况,植入4周后进行鼓膜组织苏木精-伊红、Masson染色与Ki-67免疫组化染色观察。结果与结论:①支架表征:扫描电镜显示相较于其他纳米纤维膜,高孔聚己内酯-胶原纳米纤维膜具有排列更加整齐的纳米纤维结构与更大的表面孔径,并且孔隙率更高(P<0.001);细胞活死染色显示,骨髓间充质干细胞在3种支架上附着良好,其中高孔聚己内酯-胶原纳米纤维膜上的活细胞数量多于其他两种支架;阿尔马兰染色显示,高孔聚己内酯-胶原纳米纤维膜上的骨髓间充质干细胞增殖率高于其他两种纤维膜;②动物实验:除空白对照组外,随着纤维膜植入时间的延长,其他4组大鼠鼓膜逐渐愈合,其中载细胞高孔聚己内酯-胶原组愈合速度最快;苏木精-伊红、Masson与Ki-67免疫组化染色显示,载细胞高孔聚己内酯-胶原组大鼠鼓膜厚度适中且具有3层结构,其中胶原纤维层均匀一致,与正常鼓膜相似,鼓膜修复质量好于其他纤维膜组;③结果表明:载骨髓间充质干细胞的高孔聚己内酯-胶原纳米纤维膜不仅能够快速修补鼓膜穿孔,而且新愈合鼓膜在组织结构和厚度上类似于正常鼓膜。

Collagen type Ⅳ对周围神经中再生轴突及非神经元细胞的作用和影响

本文用抗ｃｏｌｌａｇｅｎｔｙｐｅⅣ对抗体阻断ｃｏｌｌａｇｅｎｔｙｎｅⅣ的方法，研究了ｃｏｌｌａｇｅｎｔｙｐｅⅣ失活的移植神经段（长１０ｍｍ）植入大鼠坐骨神经后对再生轴突和非神经元细胞的作用和影响．实验结果显示：在移植神经段近端距近侧吻合口１ｍｍ处，术后１０ｄ抗ｃｏｌｌａｇｅｎｔｙｐｅⅣ组再生轴突数为对照组的５４％，术后１５ｄ增加到６６％，术后３０ｄ高达９４％．在移植神经段远侧距近侧吻合口９ｍｍ处，术后３０ｄ抗ｃｏｌｌａｇｅｎｔｙｐｅⅣ组再生轴突数为对照组的５８％。表明抗ｃｏｌｌａｇｅｎｔｙｐｅⅣ组再生轴突的生长启动和生长速度明显慢于对照组．巨噬细胞在移植神经段内的滞留数量抗ｃｏｌｌａｇｅｎｔｙｐｅⅣ组明显多于对照组．这些结果揭示ｃｏｌｌａｇｅｎｔｙｐｅⅣ在神经损伤和再生中对促进轴突的生长和维持神经微环境的平衡起着积极的作用．本文对ｃｏｌｌａｇｅｎｔｙｐｅⅣ在神经再生中的作用机制作了初步的分析和探讨。

海奥口腔修复膜与Bio-Gide胶原膜在即刻种植牙膜引导骨再生治疗中的疗效观察

目的:探讨海奥口腔修复膜修复及Geistlich Bio-Gide胶原膜修复在即刻种植牙膜引导骨再生的疗效。方法:选择2020年1月~2023年1月于本院行即刻种植牙的78例患者作为研究对象,采用随机数字表法分为A组(n=39)及B组(n=39)。给予A组患者海奥口腔修复膜修复,B组则采用Geistlich Bio-Gide胶原膜修复。比较两组植骨情况、术后疼痛程度[视觉模拟评分量表(visual analog scale,VAS)]、种植修复效果,并统计并发症发生情况。结果:B组患者植骨厚度、植骨高度、成骨厚度、成骨高度、水平骨成长效果及垂直骨生成效果均高于A组(P<0.05)。术后3个月B组患者VAS评分低于A组(P<0.05)。B组种植成功率高于A组,且B组并发症总发生率低于A组(P<0.05)。结论:与海奥口腔修复膜比较,Geistlich Bio-Gide胶原膜修复能改善即刻种植牙膜引导骨再生治疗患者植骨情况与种植修复效果,且有助于降低术后疼痛程度与并发症发生率。