Two-Dimension(2D) Cu-MOFs/aptamer Nanoprobe for In Situ ATP Imaging in Living Cells

Monitoring the intracellular adenosine triphosphate(ATP)level is vital for elaborating its function in physiological states.However,the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation.Herein,by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer,we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP.This nanoprobe reveals a very low fluorescence intensity because of the excellent quenching efficiency of 2D Cu-MOF nanosheets.The presence of ATP was capable to dissociate the FAM-aptamer from Cu-MOF nanosheets and resulted in an intense fluorescence signal.The Cu-MOFs/aptamer nanoprobe enables highly sensitive and selective measurement of ATP level ranging from 10μM to 800μM with a detection limit of 4.24μM.This nanosystem also further realized in situ detection of the undulation of ATP trigged by drug stimulation,depending on the selective delivery of the nanoprobe and attractive capability of resisting nonspecific displacement.The constructed nanoprobe may supply a potential platform in clinical diagnostics and biological studies.

Poly(α-L-lysine)-based nanomaterials for versatile biomedical applications:Current advances and perspectives

Poly(α-L-lysine)(PLL)is a class of water-soluble,cationic biopolymer composed ofα-L-lysine structural units.The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites.PLL-based polymers and copolymers,till date,have been extensively explored in the contexts such as antibacterial agents,gene/drug/protein delivery systems,bio-sensing,bio-imaging,and tissue engineering.This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade.The review first describes the synthesis of PLL and its derivatives,followed by the main text of their recent biomedical applications and translational studies.Finally,the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed.

Ultra-fast polymer optical fibre Bragg grating inscription for medical devices

We report the extraordinary result of rapid fibre Bragg grating inscription in doped polymer optical fibres based on polymethyl methacrylate in only 7 ms,which is two orders of magnitude faster than the inscription times previously reported.This was achieved using a new dopant material,diphenyl disulphide,which was found to enable a fast,positive refractive index change using a low ultraviolet dose.These changes were investigated and found to arise from photodissociation of the diphenyl disulphide molecule and subsequent molecular reorganization.We demonstrate that gratings inscribed in these fibres can exhibit at least a 15 times higher sensitivity than silica glass fibre,despite their quick inscription times.As a demonstration of the sensitivity,we selected a highly stringent situation,namely,the monitoring of a human heartbeat and respiratory functions.These findings could permit the inscription of fibre Bragg gratings during the fibre drawing process for mass production,allowing costeffective,single-use,in vivo sensors among other potential uses.