Two antagonistic yeasts, Thichosporon pullulans and Cryptococcus laurentii, were investigated for their biocontrol potential to blue mold rot and rhizopus rot on harvested peach fruits(Prunus persica L. Batsch, cv. Ok...Two antagonistic yeasts, Thichosporon pullulans and Cryptococcus laurentii, were investigated for their biocontrol potential to blue mold rot and rhizopus rot on harvested peach fruits(Prunus persica L. Batsch, cv. Okubao), alone or in combination with a low dose of iprodione(50 μg ml-1). The results indicated that T. pullulans and C. laurentii were effective at reducing disease incidence and severity of blue mold rot and rhizopus rot in peach fruits. Biocontrol efficacy of C. laurentii and T. pullulans were significantly enhanced by combination with a low dose of iprodione(50 μg ml-1)against blue mold and rhizopus rot in peach fruits. T. pullulans and C. laurentii combined with a low dose of iprodione(50 μg ml-1)resulted in better disease control than either iprodione or the yeasts used alone. Dipping fruits in suspensions of antagonist cells showed the similar control effect as the treatment with iprodione(500μg ml-1).展开更多
The yeasts Cryptococcus laurentii, Trichosporon pullulans, Rhodotorula glutinis andTrichosporon sp.were used to investigate their sensitivity to four fungicides anddifferent concentrations of CO2, as well as their ant...The yeasts Cryptococcus laurentii, Trichosporon pullulans, Rhodotorula glutinis andTrichosporon sp.were used to investigate their sensitivity to four fungicides anddifferent concentrations of CO2, as well as their antagonistic ability to Penicilliumexpansumand Alternariaalternata in vitro when applied with fungicide. There were significantdifferences in sensitivity to the fungicides among the different yeasts(P=0.05). R.glutinis was more sensitive to Deccocil, Iprodione and Stroby as compared to otheryeasts. Combination antagonistic yeasts with fungicide could more significantly enhancebiocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05). C.laurentii was the most effective antagonist among the four yeasts and could completelycontrol spore germination of P.expansum and A.alternata when combined with Stroby at theconcentration of 100 L L-1. The yeasts, except R.glutinis, could grow well on nutrientyeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp. showeda better adaptability to low temperature as comparedto other antagonists.展开更多
The physiological and biochemical characteristics of the yeast 3SJ with resistance against three species of postharvest diseases were identified in the pa- per, and the inhibitory effects of the yeast 3SJ on Penicilli...The physiological and biochemical characteristics of the yeast 3SJ with resistance against three species of postharvest diseases were identified in the pa- per, and the inhibitory effects of the yeast 3SJ on Penicillium expansum, Botrytis cinerea and Colletotrichum gloeosporioides during storage period was preliminarily studied. The control effects of the yeast on postharvest diseases caused by P. expansum, B. cinerea and C. gloeosporioides were measured in vivo and in vitro condi- tions. The results showed that the yeast had best control effect against P. expansum and B. cinerea at 20 ~C ; followed by the control effect against C. gloeospori- oides. Inoculation of spore suspension of yeast in apple wound could effectively inhibit rot of apple, while supematant filtrate and sterilized liquid could not inhibit rot of apple. This simultaneously indicated that the action mode of the yeast mainly was nutrition competition. The yeast was conducted traditional classification and identification based on physiological and biochemical characteristics of the yeast, and preliminarily identified to be Kloechera spp. The yeast could survive for at least one month in apple wound at 4 ~C low temperature conditions, with increased amounts. The tolerance to low temperature laid the foundation for application of yeast in low-temperature refrigeration of fruits.展开更多
Pichia membranefaciens, which was isolated from the surface of peach fruits, showed effective biocontrol capabilityagainst rhizopus rot of peach fruits. Aminoglycoside antibiotic G418 can inhibit the growth of P. memb...Pichia membranefaciens, which was isolated from the surface of peach fruits, showed effective biocontrol capabilityagainst rhizopus rot of peach fruits. Aminoglycoside antibiotic G418 can inhibit the growth of P. membranefaciens. Theminimal inhibitory concentration of G418 to P. membranefaciens in YPD medium was 100g mL-1. We constructed aphosphoglycerate kinase (PGK) promoter-driven neoR expression cassette, which was called pFL61-neo. The biocontrolyeast P. membranefaciens was transformed with pFL61-neo by lithium acetate method. Expression vector pFL61-neoconferred P. membranefaciens drug resistance to 100g mL-1 G418. The transformant could keep a high percentage ofplasmid-containing of transformant with 67.87% after 50 generations in non-selective medium. The result showed that P.membranefaciens could recognize the promoter and terminator of PGK and direct the expression of heterologous neoRgene. Expression vector pFL61-neo could exist stably in P. membranefaciens. Therefore, it is feasible to utilize G418-resistance as a dominant selectable marker for heterogenous gene expression in antagonist P. membranefaciens.展开更多
基金supported by the National Science Fund for Distinguished Young Scholars of China(30225030)the National Natural Science Foundation of China(30170663).
文摘Two antagonistic yeasts, Thichosporon pullulans and Cryptococcus laurentii, were investigated for their biocontrol potential to blue mold rot and rhizopus rot on harvested peach fruits(Prunus persica L. Batsch, cv. Okubao), alone or in combination with a low dose of iprodione(50 μg ml-1). The results indicated that T. pullulans and C. laurentii were effective at reducing disease incidence and severity of blue mold rot and rhizopus rot in peach fruits. Biocontrol efficacy of C. laurentii and T. pullulans were significantly enhanced by combination with a low dose of iprodione(50 μg ml-1)against blue mold and rhizopus rot in peach fruits. T. pullulans and C. laurentii combined with a low dose of iprodione(50 μg ml-1)resulted in better disease control than either iprodione or the yeasts used alone. Dipping fruits in suspensions of antagonist cells showed the similar control effect as the treatment with iprodione(500μg ml-1).
基金supported by the National Science Fund for Distinguished Young Scholars of China(30225030)the National Natural Science Foundation of China(30170663)the Natural Science Foundation of Beijing(6012005).
文摘The yeasts Cryptococcus laurentii, Trichosporon pullulans, Rhodotorula glutinis andTrichosporon sp.were used to investigate their sensitivity to four fungicides anddifferent concentrations of CO2, as well as their antagonistic ability to Penicilliumexpansumand Alternariaalternata in vitro when applied with fungicide. There were significantdifferences in sensitivity to the fungicides among the different yeasts(P=0.05). R.glutinis was more sensitive to Deccocil, Iprodione and Stroby as compared to otheryeasts. Combination antagonistic yeasts with fungicide could more significantly enhancebiocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05). C.laurentii was the most effective antagonist among the four yeasts and could completelycontrol spore germination of P.expansum and A.alternata when combined with Stroby at theconcentration of 100 L L-1. The yeasts, except R.glutinis, could grow well on nutrientyeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp. showeda better adaptability to low temperature as comparedto other antagonists.
基金Supported by The Forth Subject of National Scientific and Technological Support Project ( 2007BA I32B04)
文摘The physiological and biochemical characteristics of the yeast 3SJ with resistance against three species of postharvest diseases were identified in the pa- per, and the inhibitory effects of the yeast 3SJ on Penicillium expansum, Botrytis cinerea and Colletotrichum gloeosporioides during storage period was preliminarily studied. The control effects of the yeast on postharvest diseases caused by P. expansum, B. cinerea and C. gloeosporioides were measured in vivo and in vitro condi- tions. The results showed that the yeast had best control effect against P. expansum and B. cinerea at 20 ~C ; followed by the control effect against C. gloeospori- oides. Inoculation of spore suspension of yeast in apple wound could effectively inhibit rot of apple, while supematant filtrate and sterilized liquid could not inhibit rot of apple. This simultaneously indicated that the action mode of the yeast mainly was nutrition competition. The yeast was conducted traditional classification and identification based on physiological and biochemical characteristics of the yeast, and preliminarily identified to be Kloechera spp. The yeast could survive for at least one month in apple wound at 4 ~C low temperature conditions, with increased amounts. The tolerance to low temperature laid the foundation for application of yeast in low-temperature refrigeration of fruits.
基金supported by the National Science Fund for Distinguished Young Scholars of China(30225030)the National Natural Science Foundation of China(30430480).
文摘Pichia membranefaciens, which was isolated from the surface of peach fruits, showed effective biocontrol capabilityagainst rhizopus rot of peach fruits. Aminoglycoside antibiotic G418 can inhibit the growth of P. membranefaciens. Theminimal inhibitory concentration of G418 to P. membranefaciens in YPD medium was 100g mL-1. We constructed aphosphoglycerate kinase (PGK) promoter-driven neoR expression cassette, which was called pFL61-neo. The biocontrolyeast P. membranefaciens was transformed with pFL61-neo by lithium acetate method. Expression vector pFL61-neoconferred P. membranefaciens drug resistance to 100g mL-1 G418. The transformant could keep a high percentage ofplasmid-containing of transformant with 67.87% after 50 generations in non-selective medium. The result showed that P.membranefaciens could recognize the promoter and terminator of PGK and direct the expression of heterologous neoRgene. Expression vector pFL61-neo could exist stably in P. membranefaciens. Therefore, it is feasible to utilize G418-resistance as a dominant selectable marker for heterogenous gene expression in antagonist P. membranefaciens.
