Camelina(Camelina sativa)has emerged as a potential biofuel crop globally with its suitability even as a jet fuel source with 75–80%less greenhouse gas emissions compared to common petroleum fuels.The crop has origin...Camelina(Camelina sativa)has emerged as a potential biofuel crop globally with its suitability even as a jet fuel source with 75–80%less greenhouse gas emissions compared to common petroleum fuels.The crop has originated from Mediterranean region and belongs to Brassicaceae family.DIBER,DRDO has made initial and pioneer efforts in successful introduction of this crop to India and its agro-technology standardization.Being a short duration crop with lesser input requirement,it fits well in the cropping pattern of hilly states of Indian Himalaya.These areas experience fallow land due to shortage of irrigation water for growing other crops.The present study revealed that irrigation at flowering stage is more beneficial.Irrigation at this stage(if only one irrigation is available)exhibited grain yield of 1.004 kg·m^(-2)which was 21 and 80%higher over pod setting and rosette stage irrigation,respectively.The maximum seed yield was observed under treatment where irrigation was provided at all three crop growth stages(2.044 kg·m^(-2)).It was 50%higher over T6 treatment(where two irrigations were given at rosette and flowering stage)and 104%higher over T3(where only one irrigation was given at flowering stage).展开更多
Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolat...Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolated from switchgrass(Panicum virgatum), a C4 warm-season dual-purpose forage and bioenergy crop. Our results showed that recombinant PvCOMT1 enzyme protein catalyzed the methylation of 5-OH coniferyl alcohol, 5-OH coniferaldehyde(CAld5H) and 5-OH ferulic acid. Further in vitro studies indicate that CAld5H can dominate COMT-mediated reactions by inhibiting the methylation of the other substrates. Transgenic switchgrass plants generated by an RNAi approach were further employed to study the function of COMT in internode lignification. A dramatic decrease in syringyl lignin units coupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenic plants. However, the constitutive suppression of COMT in switchgrass plants altered neither the pattern of lignin deposition along the stem nor the anatomical structure of internodes. Consistent with the biochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic switchgrass plants, suggesting that CAld5H could be the optimal intermediate in the biosynthesis syringyl lignin.展开更多
基金This research was funded by the Defence Research&Development Organisation,Ministry of Defence,Govt of India.Contribution of all contributors has been acknowledged.
文摘Camelina(Camelina sativa)has emerged as a potential biofuel crop globally with its suitability even as a jet fuel source with 75–80%less greenhouse gas emissions compared to common petroleum fuels.The crop has originated from Mediterranean region and belongs to Brassicaceae family.DIBER,DRDO has made initial and pioneer efforts in successful introduction of this crop to India and its agro-technology standardization.Being a short duration crop with lesser input requirement,it fits well in the cropping pattern of hilly states of Indian Himalaya.These areas experience fallow land due to shortage of irrigation water for growing other crops.The present study revealed that irrigation at flowering stage is more beneficial.Irrigation at this stage(if only one irrigation is available)exhibited grain yield of 1.004 kg·m^(-2)which was 21 and 80%higher over pod setting and rosette stage irrigation,respectively.The maximum seed yield was observed under treatment where irrigation was provided at all three crop growth stages(2.044 kg·m^(-2)).It was 50%higher over T6 treatment(where two irrigations were given at rosette and flowering stage)and 104%higher over T3(where only one irrigation was given at flowering stage).
基金supported by the "100-Talent Program of the Chinese Academy of Sciences" foundationthe National Natural Science Foundation of China (31470390)the National Key Technologies Research & Development Program-Seven Major Crop Breeding Project (2016YFD0101803)
文摘Caffeic acid O-methyltransferase(COMT) is a crucial enzyme that mainly methylates phenylpropanoid meta-hydroxyl of C5 in the biosynthesis of syringyl lignin in angiosperms. A putative COMT, named as PvCOMT1,was isolated from switchgrass(Panicum virgatum), a C4 warm-season dual-purpose forage and bioenergy crop. Our results showed that recombinant PvCOMT1 enzyme protein catalyzed the methylation of 5-OH coniferyl alcohol, 5-OH coniferaldehyde(CAld5H) and 5-OH ferulic acid. Further in vitro studies indicate that CAld5H can dominate COMT-mediated reactions by inhibiting the methylation of the other substrates. Transgenic switchgrass plants generated by an RNAi approach were further employed to study the function of COMT in internode lignification. A dramatic decrease in syringyl lignin units coupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenic plants. However, the constitutive suppression of COMT in switchgrass plants altered neither the pattern of lignin deposition along the stem nor the anatomical structure of internodes. Consistent with the biochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic switchgrass plants, suggesting that CAld5H could be the optimal intermediate in the biosynthesis syringyl lignin.
