Heat transport properties within living biological tissues with temperature-dependent thermal properties

Understanding of the heat transport within living biological tissues is crucial to effective heat treatments. The heat transport properties of living biological tissues with temperature-dependent properties are explored in this paper. Taking into account of variable physical properties, the governing equation of temperature is first derived in the context of the dualphase-lags model(DPL). An effective method, according to the Laplace transform and a linearization technique, is then employed to solve this nonlinear governing equation. The temperature distribution of a biological tissue exposed to a pulsed heat flux on its exterior boundary, which frequently happens in various heat treatments, is predicted and analyzed. The results state that a lower temperature can be predicted when temperature dependence is considered in the heating process.The contributions of key thermal parameters are different and dependent on the ratio of phase lag and the amplitude of the exterior pulsed heat flux.

Theoretical and experimental study of the intensity distribution in biological tissues

Based on the diffusion approximate theory （DA）, a theoretical model about the distribution of the intensity of a narrow collimation beam illuminating on a semi-lnfinite biological tissue is developed. In order to verify the correctness of the model, a novel method of measuring the distributions of the intensity of light in Intralipid-10% suspension at 650 nm is presented and ts of the distributions of the distance-dependent intensity of scattering light in different directions are made. The investigations show that the results from our diffusion model are in good agreement with the experimental results beyond and in the areas around the light source, and the distance-dependent intensity in the incident direction attenuates approximately in the exponential form. Furthermore, our theoretic results indicate the anisotropic characteristics of the intensity in different directions of scattering light inside the biological tissue.

NEW PARAMETER FOR DESCRIBING AND ANALYZING THE OPTICAL-ANISOTROPIC PROPERTIES OF BIOLOGICAL TISSUES

To characterize the degree of similarity inherent to parameters of the optically uniaxial birefringent protein-fibril networks of biological tissues,a new parameter-complex degree of mutual anisotropy-has been offered.The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues has been developed.It has been shown that statistical approach to the analysis of complex degree of mutual anisotropy distributions for biological tissues in various morphological and physiological states and for different optical thicknesses appears to be more sensitive and efficient in differentiation of physiological state,as compared to investigations of complex degree of mutual polarization in the corresponding laser images.

Recent Advances in Soft Biological Tissue Manipulating Technologies

Biological soft tissues manipulation,including conventional(mechanical)and nonconventional(laser,waterjet and ultrasonic)processes,is critically required in most surgical innervations.However,the soft tissues,with their nature of anisotropic and viscoelastic mechanical properties,and high biological and heat sensitivities,are difficult to manipulated.Moreover,the mechanical and thermal induced damage on the surface and surrounding tissue during the surgery can impair the proliferative phase of healing.Thus,understanding the manipulation mechanism and the resulted surface damage is of importance to the community.In recent years,more and more scholars carried out researches on soft biological tissue cutting in order to improve the cutting performance of surgical instruments and reduce the surgery induced tissue damage.However,there is a lack of compressive review that focused on the recent advances in soft biological tissue manipulating technologies.Hence,this review paper attempts to provide an informative literature survey of the state-of-the-art of soft tissue manipulation processes in surgery.This is achieved by exploring and recollecting the different soft tissue manipulation techniques currently used,including mechanical,laser,waterjet and ultrasonic cutting and advanced anastomosis and reconstruction processes,with highlighting their governing removal mechanisms as well as the surface and subsurface damages.

QUANTITATIVE MUELLER MATRIX POLARIMETRY TECHNIQUES FOR BIOLOGICAL TISSUES

We propose and conduct both the rotating linear polarization imaging(RLPI)and Mueller matrix transformation(MMT)measurements of different biological tissue samples,and testify the capability of the Mueller matrix polarimetry for the anisotropic scattering media.The independent parameters extracted from the RLPI and MMT techniques are compared and analyzed.The tissue experimental results show that the parameters are closely related to the structural characteristics of the turbid scattering media,including the sizes of the scatterers,the angular distribution and order of alignment of thefibers.The results and conclusions in this paper may provide a potential method for the detection of precancerous and early stage cancerous tissues.Also,such studies represent the Mueller matrix transformation procedure which results in a set of parameters linking up the Mueller matrix elements to the structural and optical properties of the media.

Study and Modeling of Human Biological Tissue Exposed to High Frequency Electromagnetic Waves

The main objective of this proposed article is to provide explanations to justify the validity of the results of the studies of the interaction between the electromagnetic fields and the human body. It can also find direct applications in the characterization and modeling of the macroscopic electrical properties of the biological media for assessing the effects of fields induced by electromagnetic radiation sources in the human body to set up new standards on the Human exposure to electromagnetic fields. To do this, we have taken into account the different physical phenomena of propagation of a hyper-frequency electromagnetic plane wave and on the other hand, the experimental values in order to model the electrical behavior of human biological tissues based on an equivalent electronic circuit model composed of capacities, resistance and reel, which assimilates the biological tissues of the skin, grease, blood. This model using the characteristic impedance of the dielectric support makes it possible to evaluate the voltage induced by the electromagnetic waves of the hyper-frequencies in the studied biological system. The results of the simulations obtained from computer tools demonstrate that the hyper-frequency electromagnetic waves can result in an elevation of the electrical potential of the biological tissues. Despite this potential is a decreasing function of the penetration depth.

Anisotropic Constitutive Modeling of Compressible Biological Tissue

The anisotropic continuum stored energy density (ACSED) functional is applied for accurate constitutive modeling of biological tissues and finite element implementation without the isochoric—volumetric split, the anisotropic—isotropic split, or the anisotropic invariant split. Related stress and elasticity tensors in the reference and current configurations are worked out. A new kinematic model is derived based on the tangent Poisson’s ratio as a cubic polynomial function of stretch. The ACSED model, along with the kinematic model, accurately fits uniaxial extension test data for compressible human skin, bovine articular cartilage, and human aorta samples.

The model of electrified cell clusters in biological tissues basing on the resting potential difference

The resting potential is the potential difference that exists between the inner and outer sides of the cell membrane when the cell is not stimulated.The resting membrane potential is a key regulator of phenomena such as cell proliferation,morphogenesis,migration and differentiation.In this paper,we proposed a model of electrified cell clusters that considers the resting potential of cell clusters in the resting state.By measuring the potential difference between the inner and outer sides of biological tissues,it is verified that the cell cluster has a negative potential difference when taking the outer potential as the reference.In the absence of external conductors,the tissue is electrically neutral;while in the presence of external conductors,the positive net charges escape freely under repulsive forces and the biological tissues form a negative electrical equilibrium system.The model proposed in this study explored the potential situation above the cellular level in the resting state,providing a new perspective for the research on resting potential.

A novel on-tissue cycloaddition reagent for mass spectrometry imaging of lipid C=C position isomers in biological tissues

Application of matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results.However, the presence of structural isomers varying in C=C double bond(DB) locations makes isomerresolved MSI an urgent need. Herein, we introduce a new type of light-driven on-tissue [2 + 2] cycloaddition reaction coupled with MALDI-MS/MS imaging to identify lipid DB position isomers and their spatial signatures in biological tissues. 3-Benzoylpyridine was introduced as a novel derivatization reagent, and it exhibited great reactivity toward lipid C=C bond to form oxetanes under both ultraviolet light and visible light irradiation. With this approach, DB position isomers of lipids were imaged with highly differential levels in distinct regions of rat brain, providing an accurate and spatially resolved approach to study tissue lipidomics.

Laser-enhanced thermal effects during focused ultrasound radiation on biological tissues

The thermal effects induced by a moderate intensity focused ultrasound and enhanced by combined laser pulses for bio-tissues and tissue-phantom are studied experimentally and theoretically. At first, the heating effects of bio-tissues and tissue-phantom induced by ultrasound and enhanced by laser are measured experimentally. The heating processes induced by attenuations of focused ultrasonic waves and cavitation effects of the focused ultrasound and combined laser are analyzed theoretically. By analyzing the mechanisms of these effects, it is found that the laser nucleation makes the cavitation bubble generation more easily, which can effectively enhance the ultrasonic cavitation effects, and then enhance the thermal effects of the samples. On the other hand, to evaluate quantitatively the heating processes induced by the focused ultrasound and enhanced by the pulsed laser, by fitting the theoretical calculations to the experimental results, the corresponding cavitation bubbles and rising temperatures induced by the focused ultrasound with and without laser can be estimated approximately.

Measurement of the Elasticity of Biological Soft Tissue of Finite Thickness

Elasticity is of profound significance to evaluating the function of a biological soft tissue. When the elasticity of a tissue is macroscopically changed, it means that the biological function of the tissue is abnormal and some disease or injury may occur. In the present work, an elastometer is developed to measure the elasticity of biological soft tissues. The measurement is based on the indentation method and the force is measured by the bending of the cantilever. The force-indentation data of the soft tissue is experimentally measured by this elastometer and Young＇s modulus of the tissue is calculated using the Hertz-Sneddon model. For comparison, a numerical model for the indentation method is established using the finite element method. The difference between the actual modulus and the measured modulus is discussed. The effect of the thickness of the specimen on the measurement is investigated. Young＇s moduli of beef, porcine liver and porcine kidney are experimentally measured. The results indicate that our elastometer is effective in measuring Young＇s modulus of a soft tissue quantitatively.

Coagulation and ablation of biological soft tissue byquantum cascade laser with peak wavelength of 5.7μm

Molecules such as water,proteins and lipids that are contained in biological tissue absorb mid-infrared(MR)light,which allows such light to be used in laser surgical treatment.Esters,amides and water exhibit strong absorption bands in the 5-7μm wavelength range,but at present there are no lasers in clinical use that can emit in this range.Therefore,the present study focused on thequantum cascade laser(QCL),which is a new type of semiconductor laser that can emit at MIRwavelengths and has recentiy achieved high output power.A high-power QCL with a peakwavelength of 5.7μm was evaluated for use as a laser scalpel for ablating biological soft tissue.The interaction of the laser beam with chicken breast tissue was compared to a conventional CO_(2) laser,based on surface and cross-sectional images.The QCL was found to have sufficient power to ablate soft tissue,and its coagulation,carbonization and ablation effects were similar to those forthe CO_(2) laser.The QCL also induced comparable photothermal effects because it acted as apseudo-continuous wave laser due to its low peak power.A QCL can therefore be used as an effective laser scalpel,and also offers the possibility of less invasive treatment by targeting specificabsorption bands in t he MIR region.

Varying of up-conversion nanoparticles luminescence from the muscle tissue depth during the compression

The current work is focused on the study of optical clearing of skeletal muscles under local compression.The experiments were performed on in vitro bovine skeletal muscle.The time dependence of optical clearing was studied by monitoring the luminescence intensity of NaYF_(4)∶Er,Yb upconverting particles located under tissue layers.This study shows the possibility to use upconverting nanoparticles(UCNPs)both for studying the dynamics of the optical clearing of biological tissue under compression and to detect moments of cell wall damage under excessive pressure.The advantage of using UCNPs is the presence of several bands in their luminescence spectra,located both at close wavelengths and far apart.

Analysis, Sources and Study of the Biological Consequences of Electromagnetic Pollution

The actions and health effects of electromagnetic fields in the radio frequency (RF) domains, referred to as radio frequencies and HV transmission networks have been studied for several decades. Following the appearance of questions and debates within the population, the actions and potential effects of radiofrequency and HV transport networks on health, in connection with the development of new wireless technologies, are generating a certain revival of interest. Thus, the increasing exposure to electromagnetic fields and the concerns of the public have led health organizations to undertake large-scale research programs to respond to the concerns expressed. These research programs have contributed to significantly increasing the number of studies on the actions and effects of electromagnetic pollution as well as their consequences on living beings. The objective of our research is focused on the analysis, sources, and study of the biological consequences of electromagnetic pollution. To do this, we have used physical laws and theorems, in particular Maxwell-Ampère, Maxwell-Gauss, Maxwell-Faraday, and Ohm’s law, to model the interactions between electromagnetic fields and living matter. In this article we have chosen the approach based on the electrical model of human biological tissue, taking into account on the one hand the physical phenomena of the propagation of an electromagnetic microwave plane wave in the range from 0 to 300GHz and on the other hand, the experimental values to simulate the relaxations α, β and γ and the impedance of the biological tissue faced with the variation of the frequency of propagation of the electromagnetic waves to identify the biological consequences relating thereto. The results obtained in the literature show the linear dependence of bio-impedance on frequency, these observations suggest that the tissue can be physiologically stressed at high frequencies. This can cause biological consequences for humans. The 2D simulation based on the proposed model has been developed as well as the verification of the consistency of the different mathematical models, by comparing the fractal dimensions of the results of the program with those of the figures obtained experimentally.

Acoustic dipole radiation model for magnetoacoustic tomography with magnetic induction

An acoustic dipole radiation model for magnetoacoustic tomography with magnetic induction （MAT-MI） is pro- posed, based on the analyses of one-dimensional tissue vibration, three-dimensional acoustic dipole radiation and acoustic waveform detection with a planar piston transducer. The collected waveforms provide information about the conductiv- ity boundaries in various vibration intensities and phases due to the acoustic dipole radiation pattern. Combined with the simplified back projection algorithm, the conductivity configuration of the measured layer in terms of shape and size can be reconstructed with obvious border stripes. The numerical simulation is performed for a two-layer cylindrical phantom model and it is also verified by the experimental results of MAT-MI for a tissue-like sample phantom. The proposed model suggests a potential application of conductivity differentiation and provides a universal basis for the further study of conductivity reconstruction for MAT-MI.

Fast Microwave-Induced Thermoacoustic Tomography Based on Multi-Element Phase-Controlled Focus Technique

We develop a fast microwave-induced thermoacoustic tomography system based on a 320-element phase-controlled focus linear transducer array. A 1.2-GHz microwave generator transmits microwave with a pulse width of 0.5 μs and an incident energy density of 0. 45 mJ/cm^2, and the microwave energy is delivered by a rectangular waveguide with a cross section of （80.01 ± 0.02） × 10^-4 m^2. Compared to single transducer collection, the system with the multi-element linear transducer array can eliminate the mechanical rotation of the transducer, hence can effectively reduce the image blurring and improve the image resolution. Using a phase-controlled focus technique to collect thermoacoustic signals, the data need not be averaged because of a high signal-to-noise ratio, resulting in a total data acquisition time of less than 5 s. The system thus provides a rapid and reliable approach to thermoacoustic imaging, which can potentially be developed as a powerful diagnostic tool for early-stage breast caners.

EPR spectroscopy of whole blood and blood components:can we diagnose abnormalities?

This mini-review gives a brief account of the emergence of the electron paramagnetic resonance(EPR)spectroscopy in the second half of the 20th century and reports the continuous wave EPR spectroscopy studies on human and animal blood.The question posed by this review is whether the EPR spectroscopy in the form it appeared 70 years ago is still able to provide useful information about different pathological conditions in humans,particularly in the area of diagnosis.

Integrated System for Combined Optical Coherence Tomography-Raman Spectroscopy of Neocaridina denticulate sinensis

Optical coherence tomography(OCT)and Raman spectroscopy(RS)can be complementary biological tissue optical analysis methods.To study the internal structure and tissue compositions of biological samples,an OCT-RS system was built to carry out OCT section imaging and RS analysis in common.Neocaridina denticulate sinensis were collected regularly for morphological observation by OCT imaging and biochemical investigation based on the Raman spectra.The internal structure of the N.denticulate sinensis was imaged by OCT,and the morphology of the tissues and the position in the body were distinguished according to the gray scale changes.The imaging depth along the vertical direction of Z-axis in N.denticulate sinensis is about 1.60 mm.RS detection was selectively performed based on the OCT images.The main Raman peaks of the rostrum,the cephalothorax,the abdominal segment,and the telson section are at 1006,1156,1447,1491 and 1515 cm-1,which are identified as proteins and amino acids.The presence of 1497 cm-1 at the cephalothorax is different from other parts,probably due to the presence of organs such as ovary,whose compositions are different from those of other tissues.The combination of optical coherence tomography and Raman spectroscopy can provide information about morphological and biochemical features of tissues,and has potential applications in biomedical detection and imaging.

Nanoindentation of soft solids by a fla punch

Measuring the surface tension and elastic mod- ulus of soft materials and biological tissues under different physiological and pathological conditions is of significance for understanding various phenomena associated with defor- mation. In this paper, the nanoindentation of a circular fiat punch on a soft solid is analyzed with the influence of surface tension. By solving the corresponding singular integral equa- tion, the relation between load and indent depth is obtained. When the radius of the flat punch shrinks to the same order as the ratio of surface tension to elastic modulus, surface ten- sion significantly affects the indentation load-depth relation, which provides a facile method to measure surface tension in soft solids and biological tissues.