Industrial-scale bioprocessing underpins much of the production of pharmaceuticals,nutraceuticals,food,and beverage processing industries of the modern world.The proftability of these processes increasingly leverages ...Industrial-scale bioprocessing underpins much of the production of pharmaceuticals,nutraceuticals,food,and beverage processing industries of the modern world.The proftability of these processes increasingly leverages the economies of scale and scope that are critically dependent on the product yields,titers,and productivity.Most of the processes are controlled using classical control approaches and represent over 90%of the industrial controls used in bioprocessing industries.However,with the advances in the production processes,especially in the biopharmaceutical and nutraceutical industries,monitoring and control of bioprocesses such as fermentations with GMO organisms,and downstream processing has become increasingly complex and the inadequacies of the classical and some of the modern control systems techniques is becoming apparent.Therefore,with increasing research complexity,nonlinearity,and digitization in process,there has been a critical need for advanced process control that is more efective,and easier process intensifcation and product yield(both by quality and quantity)can be achieved.In this review,industrial aspects of a process and automation along with various commercial control strategies have been extensively discussed to give an insight into the future prospects of industrial development and possible new strategies for process control and automation with a special focus on the biopharmaceutical industry.展开更多
Chinese traditional medicine wastewater, rich in macromolecule and easy to foam in aerobic biodegradation such as Glycosides, was treated by two identical bench-scale aerobic submerged membrane bioreactors （SMBRs） o...Chinese traditional medicine wastewater, rich in macromolecule and easy to foam in aerobic biodegradation such as Glycosides, was treated by two identical bench-scale aerobic submerged membrane bioreactors （SMBRs） operated in parallel under the same feed, equipped with the same electronic control backwashing device. One was used as the control SMBR （CSMBR） while the other was dosed with powdered activated carbon （PAC） （PAC-amended SMBR, PSMBR）. The backwashing interval was 5 min. One suction period was about 90 min by adjusting preestablished backwashing vacuum and pump frequency. The average flux of CSMBR during a steady periodic state of 24 d （576 h） was 5.87 L/h with average hydraulic residence time （HRT） of 5.97 h and that of PSMBR during a steady periodic state of 30 d （720 h） was 5.85 L/h with average HRT of 5.99 h. The average total chemical oxygen demand （COD） removal efficiency of CSMBR was 89.29% with average organic loading rate （OLR） at 4.16 kg COD/（m^3.d） while that of PSMBR was 89.79% with average OLR at 5.50 kg COD/（m^3.d）. COD concentration in the effluent of both SMBRs achieved the second level of the general wastewater effluent standard GB8978-1996 for the raw medicine material industry （300 mg/L）. Hence, SMBR with electronic control backwashing was a viable process for medium-strength Chinese traditional medicine wastewater treatment. Moreover, the increasing rates of preestablished backwashing vacuum, pump frequency, and vacuum and flux loss caused by mixed liquor in PSMBR all lagged compared to those in CSMBR; thus the actual operating time of the PSMBR system without membrane cleaning was extended by up to 1.25 times in contrast with the CSMBR system, and the average total COD removal efficiency of PSMBR was enhanced with higher average OLR.展开更多
Shortcut nitrification for ammonium-rich wastewater is energy-saving and cost-effective procedure that has become one of the hotspots in the field of biological denitrogenation. An orthogonal experiment was performed ...Shortcut nitrification for ammonium-rich wastewater is energy-saving and cost-effective procedure that has become one of the hotspots in the field of biological denitrogenation. An orthogonal experiment was performed to study the combined effects of operational parameters on the performance of internal-loop airlift bioreactor for shortcut nitrification. The optimum operational parameters for the shortcut nitrification were fotmd as temperature 35 ℃, pH 8.0, dissolved oxygen concentration 1.0 mg/L, ammonium concentration 4 mmol/L and HRT 16 h, which have different influence on the performance of shortcut nitrification reactor. The pH, temperature and dissolved oxygen concentration have significant bearing on the process. The results showed that the shortcut nitrification reactor could be successfully started up within 42 d, and the reactor performance is steady with minimum NO2-/NOx- of 85.2%, maximum 93.4% and average value of 91.4% in effluent. Based on the analysis of experimental data, a new control strategy named “priority ＋ combination” for shortcut nitrification was suggested. Through this strategy, the startup and operation of shortcut nitrification for ammonium-rich sludge digester liquids were optimized. The control strategy works well to keep the reactor operation in steady state and in achieving high-efficiency for shortcut nitrification.展开更多
This study investigated the use of microbial analysis as a bioremediation option for remediating petroleum sludge, which is part of the waste stream generated in the petroleum industry. The aim is to reduce environmen...This study investigated the use of microbial analysis as a bioremediation option for remediating petroleum sludge, which is part of the waste stream generated in the petroleum industry. The aim is to reduce environmental burden caused by the discharge of untreated sludge. Sludge sample was cultured in other to isolate microorganisms for the sludge treatment. The selected strain of the organisms after screening were Aspergillus flavus, Aspergillus niger, Verticillus sp, Penicillum sp, and Microsporium audouinii. Bioreactors (labeled A, B, C, D and O) were designed for the treatment of petroleum sludge. These reactors contain 2.0 × 10<sup>-2</sup> m<sup>3</sup> of the diluted sludge samples and the isolated organisms for the treatment process. On a weekly basis, the control reactors received 1.5 × 10<sup>-3</sup> m<sup>3</sup> of fresh and saline water respectively. After 12 weeks of treatment, sludge physicochemical characteristics showed distinct variations. From the result, reactor D was the best in terms of remediating the sludge as compared to other reactors. Friedman non-parametric test was performed to check if the weeks of treatment affected the reduction of the total hydrocarbon content (THC) in the five reactors and also checked for significant differences in the THC after treatments. The drop in the THC of the treated sludge ranged from 56.0% to 67.3%. These results showed the possibility of enhanced biodegradation of petroleum sludge by hydrocarbon utilizing microorganisms (fungi).展开更多
提出了一种基于先进过程控制(Advanced Process Control,APC)技术的综合控制方案在生物型人工肝支持系统(BAL)中的应用;对系统中被控物理量之间的相互作用规律进行了定性分析,按照物料平衡算式使之关联匹配,经过PI调节后,根据使用经验...提出了一种基于先进过程控制(Advanced Process Control,APC)技术的综合控制方案在生物型人工肝支持系统(BAL)中的应用;对系统中被控物理量之间的相互作用规律进行了定性分析,按照物料平衡算式使之关联匹配,经过PI调节后,根据使用经验进行离线结合在线的预测控制;实验结果表明:尽管生物反应器内存在着诸多非线性因素,且具有很强的耦合性及滞后特性,该方法仍取得了较好的控制效果,达到了BAL的使用要求。展开更多
文摘Industrial-scale bioprocessing underpins much of the production of pharmaceuticals,nutraceuticals,food,and beverage processing industries of the modern world.The proftability of these processes increasingly leverages the economies of scale and scope that are critically dependent on the product yields,titers,and productivity.Most of the processes are controlled using classical control approaches and represent over 90%of the industrial controls used in bioprocessing industries.However,with the advances in the production processes,especially in the biopharmaceutical and nutraceutical industries,monitoring and control of bioprocesses such as fermentations with GMO organisms,and downstream processing has become increasingly complex and the inadequacies of the classical and some of the modern control systems techniques is becoming apparent.Therefore,with increasing research complexity,nonlinearity,and digitization in process,there has been a critical need for advanced process control that is more efective,and easier process intensifcation and product yield(both by quality and quantity)can be achieved.In this review,industrial aspects of a process and automation along with various commercial control strategies have been extensively discussed to give an insight into the future prospects of industrial development and possible new strategies for process control and automation with a special focus on the biopharmaceutical industry.
基金Project supported by the Hi-Tech Research and Development Program(863)of China(No. 2002AA601310).
文摘Chinese traditional medicine wastewater, rich in macromolecule and easy to foam in aerobic biodegradation such as Glycosides, was treated by two identical bench-scale aerobic submerged membrane bioreactors （SMBRs） operated in parallel under the same feed, equipped with the same electronic control backwashing device. One was used as the control SMBR （CSMBR） while the other was dosed with powdered activated carbon （PAC） （PAC-amended SMBR, PSMBR）. The backwashing interval was 5 min. One suction period was about 90 min by adjusting preestablished backwashing vacuum and pump frequency. The average flux of CSMBR during a steady periodic state of 24 d （576 h） was 5.87 L/h with average hydraulic residence time （HRT） of 5.97 h and that of PSMBR during a steady periodic state of 30 d （720 h） was 5.85 L/h with average HRT of 5.99 h. The average total chemical oxygen demand （COD） removal efficiency of CSMBR was 89.29% with average organic loading rate （OLR） at 4.16 kg COD/（m^3.d） while that of PSMBR was 89.79% with average OLR at 5.50 kg COD/（m^3.d）. COD concentration in the effluent of both SMBRs achieved the second level of the general wastewater effluent standard GB8978-1996 for the raw medicine material industry （300 mg/L）. Hence, SMBR with electronic control backwashing was a viable process for medium-strength Chinese traditional medicine wastewater treatment. Moreover, the increasing rates of preestablished backwashing vacuum, pump frequency, and vacuum and flux loss caused by mixed liquor in PSMBR all lagged compared to those in CSMBR; thus the actual operating time of the PSMBR system without membrane cleaning was extended by up to 1.25 times in contrast with the CSMBR system, and the average total COD removal efficiency of PSMBR was enhanced with higher average OLR.
文摘Shortcut nitrification for ammonium-rich wastewater is energy-saving and cost-effective procedure that has become one of the hotspots in the field of biological denitrogenation. An orthogonal experiment was performed to study the combined effects of operational parameters on the performance of internal-loop airlift bioreactor for shortcut nitrification. The optimum operational parameters for the shortcut nitrification were fotmd as temperature 35 ℃, pH 8.0, dissolved oxygen concentration 1.0 mg/L, ammonium concentration 4 mmol/L and HRT 16 h, which have different influence on the performance of shortcut nitrification reactor. The pH, temperature and dissolved oxygen concentration have significant bearing on the process. The results showed that the shortcut nitrification reactor could be successfully started up within 42 d, and the reactor performance is steady with minimum NO2-/NOx- of 85.2%, maximum 93.4% and average value of 91.4% in effluent. Based on the analysis of experimental data, a new control strategy named “priority ＋ combination” for shortcut nitrification was suggested. Through this strategy, the startup and operation of shortcut nitrification for ammonium-rich sludge digester liquids were optimized. The control strategy works well to keep the reactor operation in steady state and in achieving high-efficiency for shortcut nitrification.
文摘This study investigated the use of microbial analysis as a bioremediation option for remediating petroleum sludge, which is part of the waste stream generated in the petroleum industry. The aim is to reduce environmental burden caused by the discharge of untreated sludge. Sludge sample was cultured in other to isolate microorganisms for the sludge treatment. The selected strain of the organisms after screening were Aspergillus flavus, Aspergillus niger, Verticillus sp, Penicillum sp, and Microsporium audouinii. Bioreactors (labeled A, B, C, D and O) were designed for the treatment of petroleum sludge. These reactors contain 2.0 × 10<sup>-2</sup> m<sup>3</sup> of the diluted sludge samples and the isolated organisms for the treatment process. On a weekly basis, the control reactors received 1.5 × 10<sup>-3</sup> m<sup>3</sup> of fresh and saline water respectively. After 12 weeks of treatment, sludge physicochemical characteristics showed distinct variations. From the result, reactor D was the best in terms of remediating the sludge as compared to other reactors. Friedman non-parametric test was performed to check if the weeks of treatment affected the reduction of the total hydrocarbon content (THC) in the five reactors and also checked for significant differences in the THC after treatments. The drop in the THC of the treated sludge ranged from 56.0% to 67.3%. These results showed the possibility of enhanced biodegradation of petroleum sludge by hydrocarbon utilizing microorganisms (fungi).
文摘提出了一种基于先进过程控制(Advanced Process Control,APC)技术的综合控制方案在生物型人工肝支持系统(BAL)中的应用;对系统中被控物理量之间的相互作用规律进行了定性分析,按照物料平衡算式使之关联匹配,经过PI调节后,根据使用经验进行离线结合在线的预测控制;实验结果表明:尽管生物反应器内存在着诸多非线性因素,且具有很强的耦合性及滞后特性,该方法仍取得了较好的控制效果,达到了BAL的使用要求。
