The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly incre...The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly increased with increasing germination time upto 120 h depending upon the treatment (P【0.05). Maximum amounts of ascorbic acid, 22.32 and 16.84 mg/100g, were found in the 0.10 kGy sample after 120 h of germination in tap and distilled water respectively. However, a radiation dose of 0.15 kGy resulted in the development of maximum values of riboflavin, 11.40 and 11.38 μ g/g, on germination in tap and distilled water respectively. A significant linear relation (r = 0-954 to 0.997) was observed between the biosyntheses of these vitamins and the germination time upto 120 h of irradiated and unirradiated chickpea seed (P【0.05).展开更多
Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging metho...Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging method for accurate and rapid diagnosis of cancer cells by introducing aqueous Fe^2＋ and Zn^2＋ ions into cancer cells （i.e., HeLa, U87, and HepG2 cancer cells）. We found that the biocompatible metal ions Fe^2＋ and Zn^2＋ forced the cancer cells to spontaneously synthesize fluorescent ZnO nanoclusters and magnetic Fe3O4 nanoclusters. These clusters could then be used for multimodal cancer imaging by combining fluorescence imaging with magnetic resonance imaging and computed tomography imaging. Meanwhile, for normal cells （i.e., L02） and tissues, neither fluorescence nor any other obvious difference could be detected between pre- and post-injection. This multimodal bioimaging strategy based on the in situ biosynthesized Zn＆Fe oxide nanoclusters might therefore be useful for early cancer diagnosis and therapy.展开更多
文摘The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly increased with increasing germination time upto 120 h depending upon the treatment (P【0.05). Maximum amounts of ascorbic acid, 22.32 and 16.84 mg/100g, were found in the 0.10 kGy sample after 120 h of germination in tap and distilled water respectively. However, a radiation dose of 0.15 kGy resulted in the development of maximum values of riboflavin, 11.40 and 11.38 μ g/g, on germination in tap and distilled water respectively. A significant linear relation (r = 0-954 to 0.997) was observed between the biosyntheses of these vitamins and the germination time upto 120 h of irradiated and unirradiated chickpea seed (P【0.05).
基金This work is supported by the National High-tech R＆D Program of China （No. 2015AA020502） and the National Natural Science Foundation of China （Nos. 81325011, 21327902 and 21175020）. M. S. acknowledges support from the NSF-PREM program （No, DRM-1523588）.
文摘Early diagnosis remains highly important for efficient cancer treatment, and hence, there is significant interest in the development of effective imaging strategies. This work reports a new multimodal bioimaging method for accurate and rapid diagnosis of cancer cells by introducing aqueous Fe^2＋ and Zn^2＋ ions into cancer cells （i.e., HeLa, U87, and HepG2 cancer cells）. We found that the biocompatible metal ions Fe^2＋ and Zn^2＋ forced the cancer cells to spontaneously synthesize fluorescent ZnO nanoclusters and magnetic Fe3O4 nanoclusters. These clusters could then be used for multimodal cancer imaging by combining fluorescence imaging with magnetic resonance imaging and computed tomography imaging. Meanwhile, for normal cells （i.e., L02） and tissues, neither fluorescence nor any other obvious difference could be detected between pre- and post-injection. This multimodal bioimaging strategy based on the in situ biosynthesized Zn＆Fe oxide nanoclusters might therefore be useful for early cancer diagnosis and therapy.
