We evaluated the potential of orally fed new food formulations to inhibit biomarkers reported to be involved in the causes of allergic asthma in mice. Asthma, a serious non-communicable disease, affects both adults an...We evaluated the potential of orally fed new food formulations to inhibit biomarkers reported to be involved in the causes of allergic asthma in mice. Asthma, a serious non-communicable disease, affects both adults and children and can be undertreated. New functional foods could provide therapeutic approaches. Here, the anti-asthma mechanism of a new functional food and three isolated fractions produced by bioprocessing black rice bran with shiitake mushroom mycelia was evaluated in mast cells, B cells, and orally fed mice and compared with non-bioprocessed black rice bran. In vitro, the treatments inhibited RBL-2H3 cell degranulation and immunoglobulin E (IgE) production. The in vitro anti-asthma effects were confirmed in orally fed mice following asthma induction by alumina and chicken egg ovalbumin (OVA). The suppression of asthma resulted from the inhibition of inflammation- and immune-related substances, including OVA-specific IgE, thymic stromal lymphopoietin, eotaxin, leukotriene C4, prostaglandin D2, and vascular cell adhesion molecule-1 in bronchoalveolar lavage fluid and serum. The treatment also reversed the thickening of the lung airway wall. The inflammation and asthma inhibition seems to be regulated by the balance of the T-helper cells’ Th1/Th2 immune response and the inhibition of multiple biomarkers associated with the cause of asthma. Future human clinical studies with adults and children should determine the potential therapeutic value of the anti-asthma effects of the new functional foods.展开更多
Objective: To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.Methods: Purposely, rat modelling was carried out for 2 months by designing three stu...Objective: To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.Methods: Purposely, rat modelling was carried out for 2 months by designing three studies i.e. study Ⅰ(normal rats), study Ⅱ(hyperglycaemic rats) and study Ⅲ(hypercholesterolemic rats). Each study was further divided into three groups based on diet i.e.control, functional diet(contained extract of black cumin prepared by using conventional solvent) and nutraceutical diet(contained extract of black cumin prepared by supercritical fluid extraction system).Results: During whole trial, an abating trend was observed in the level of serum cholesterol with maximum reduction(12.8%) in nutraceutical group of study Ⅲ. Low density lipoprotein and triglyceride level was also lowered maximum in study Ⅲ as17.1% and 11.6%, respectively. Whereas, highest decline in glucose level was in nutraceutical group of study Ⅱ as 11.2%.Conclusions: Inclusion of black cumin extracts in diet significantly lowers the occurrence of hyperglycaemia and hypercholesterolaemia. Furthermore, hypoglycaemic and hypocholesterolemic potential of nutraceutical diet is more prominent as compared to functional diet.展开更多
文摘We evaluated the potential of orally fed new food formulations to inhibit biomarkers reported to be involved in the causes of allergic asthma in mice. Asthma, a serious non-communicable disease, affects both adults and children and can be undertreated. New functional foods could provide therapeutic approaches. Here, the anti-asthma mechanism of a new functional food and three isolated fractions produced by bioprocessing black rice bran with shiitake mushroom mycelia was evaluated in mast cells, B cells, and orally fed mice and compared with non-bioprocessed black rice bran. In vitro, the treatments inhibited RBL-2H3 cell degranulation and immunoglobulin E (IgE) production. The in vitro anti-asthma effects were confirmed in orally fed mice following asthma induction by alumina and chicken egg ovalbumin (OVA). The suppression of asthma resulted from the inhibition of inflammation- and immune-related substances, including OVA-specific IgE, thymic stromal lymphopoietin, eotaxin, leukotriene C4, prostaglandin D2, and vascular cell adhesion molecule-1 in bronchoalveolar lavage fluid and serum. The treatment also reversed the thickening of the lung airway wall. The inflammation and asthma inhibition seems to be regulated by the balance of the T-helper cells’ Th1/Th2 immune response and the inhibition of multiple biomarkers associated with the cause of asthma. Future human clinical studies with adults and children should determine the potential therapeutic value of the anti-asthma effects of the new functional foods.
基金partially supported by Higher Education Commission,Pakistan under Pak-US Science and Technology Cooperation Program Phase IV(Project Grant No.10/01/10-09/30/12)“Establishment of Functional and Nutraceutical Food Research Section at the National Institute of Food Science and Technology,University of Agriculture,Faisalabad,Pakistan”
文摘Objective: To explore the hypoglycaemic and hypocholesterolemic potential of conventional and supercritical extracts of black cumin.Methods: Purposely, rat modelling was carried out for 2 months by designing three studies i.e. study Ⅰ(normal rats), study Ⅱ(hyperglycaemic rats) and study Ⅲ(hypercholesterolemic rats). Each study was further divided into three groups based on diet i.e.control, functional diet(contained extract of black cumin prepared by using conventional solvent) and nutraceutical diet(contained extract of black cumin prepared by supercritical fluid extraction system).Results: During whole trial, an abating trend was observed in the level of serum cholesterol with maximum reduction(12.8%) in nutraceutical group of study Ⅲ. Low density lipoprotein and triglyceride level was also lowered maximum in study Ⅲ as17.1% and 11.6%, respectively. Whereas, highest decline in glucose level was in nutraceutical group of study Ⅱ as 11.2%.Conclusions: Inclusion of black cumin extracts in diet significantly lowers the occurrence of hyperglycaemia and hypercholesterolaemia. Furthermore, hypoglycaemic and hypocholesterolemic potential of nutraceutical diet is more prominent as compared to functional diet.
