The work demonstrates an example of application of ^252Cf PDMS （plasma desorption mass spectrometry） to analysis of lipid profile of carotene-containing Blakeslea trispora biomass. It was shown that this method allo...The work demonstrates an example of application of ^252Cf PDMS （plasma desorption mass spectrometry） to analysis of lipid profile of carotene-containing Blakeslea trispora biomass. It was shown that this method allows identification of the individual components in the crude multicomponent mixtures not subjected to previous chromatographic separation or enrichment. Using a very simple pretreatment by different extractants permits to select preferable lipid fractions of microorganisms.展开更多
Ion beam bioengineering technology as a new mutation approach has been widely used in the biological breeding field. In this paper the application of low energy nitrogen ion implantation in the β-carotene producing s...Ion beam bioengineering technology as a new mutation approach has been widely used in the biological breeding field. In this paper the application of low energy nitrogen ion implantation in the β-carotene producing strain, Blakeslea trispora（-） was investigated. The effects of different fermentation conditions on β-carotene production by a high yield strain were examined. Results showed that two β-carotene high yielding strains B.trispora（-） BH3-701 and BH3-728 were screened out and the averaged production of β-carotene was raised by 178.7% and 164.6% respectively after five passages in the shaking flasks. Compared with the original strain, the highest yield strain BH3-701 was potent in accumulating β-carotene, especially in the later stage, and greatly increased production efficiency.展开更多
文摘The work demonstrates an example of application of ^252Cf PDMS （plasma desorption mass spectrometry） to analysis of lipid profile of carotene-containing Blakeslea trispora biomass. It was shown that this method allows identification of the individual components in the crude multicomponent mixtures not subjected to previous chromatographic separation or enrichment. Using a very simple pretreatment by different extractants permits to select preferable lipid fractions of microorganisms.
文摘Ion beam bioengineering technology as a new mutation approach has been widely used in the biological breeding field. In this paper the application of low energy nitrogen ion implantation in the β-carotene producing strain, Blakeslea trispora（-） was investigated. The effects of different fermentation conditions on β-carotene production by a high yield strain were examined. Results showed that two β-carotene high yielding strains B.trispora（-） BH3-701 and BH3-728 were screened out and the averaged production of β-carotene was raised by 178.7% and 164.6% respectively after five passages in the shaking flasks. Compared with the original strain, the highest yield strain BH3-701 was potent in accumulating β-carotene, especially in the later stage, and greatly increased production efficiency.
