Blood Calcium as a Protective Factor against Traumatic Fracture

Purpose: This study aims to investigate the predictive value of blood calcium in the prognosis of traumatic fracture. Methods: A retrospective experimental design was employed, 112 cases (52 non-fracture and 60 traumatic fracture) were randomly selected. The type of fracture complies with WHO-recommended (2019) diagnostic criteria for osteoporosis combined with fracture. The blood pressure (BP) was measured by OMRON’s HEM-7136 model electronic blood pressure monitor. Blood calcium (Ca2+), and blood phosphorus (P) values were measured using Colorimetric Roche kits on a Roche/Hitachi fully automated biochemical analyzer. Data collection and analysis followed. Results: Higher levels of age, systolic and diastolic blood pressures were found in the traumatic fracture group compared to the control group, whereas weight, height, and blood phosphorus did not differ significantly (P adjusting for age, systolic blood pressure, diastolic blood pressure, and blood phosphorus, binary logistic regression analysis revealed that blood calcium was a protective factor against traumatic fracture (β = -26.85, OR = 0.00, 95% CI = 0.00 -0.02, P = 0.022). Conclusion: The findings suggest that high and low blood calcium levels may serve as useful indicators in predicting the prognosis of fractures resulting from trauma.

Altered Calcium Handling in Peripheral Nerve Terminals and Blood Vessels in Spontaneously Hypertensive Rats

The present study was designed to clarify the roles of N-type and P/Q-type calcium channels in the increased sympathetic activity of spontaneously hypertensive rats (SHR/Izm). We also tested in SHR/Izm the contribution to increased vascular tone of α1A adrenoceptor-linked L-type calcium channels and α1B receptor-mediated calcium mobilization from the sarcoplasmic reticulum. Methods: Six-week-old SHR/Izm and Wistar-Kyoto rats (WKY/Izm) were used. A superior mesenteric arterial preparation was electrically stimulated before and after treatment with ω-conotoxin GVIa (N-type calcium channel blocker [CgTX]) and ω-agatoxinIVa (P/Q-type calcium channel blocker [AgaTX]). Pressor response to norepinephrine was measured before and after treatment with the α1A blocker WB-4101 and the α1B blocker chloroethyl clonidine (CEC). To determine the intracellular calcium store size, the effects of ryanodine on pressor response and caffeine-induced vascular contraction were also tested. Results: Norepinephrine overflow evoked by electrical stimulation was increased in SHR/Izm. CgTX but not AgaTX suppressed the increased NE overflow in SHR/Izm. WB-4101 suppressed the pressor response to norepinephrine in SHR/Izm but not WKY/Izm rats. CEC had no effects on pressor response to norepinephrine in both types of rats. Caffeine-induced contraction to a high potassium-induced maximal contraction ratio was reduced in SHR/Izm. The effect of ryanodine on pressor response was reduced in SHR/Izm. Conclusion: N-type calcium channels but not P/Q-type calcium channels play an important role in the increased sympathetic tone in SHR/ Izm. Although α1A adrenoceptor-linked L-type calcium channels contribute to the increased vascular tone, the intracellular calcium store size was reduced in SHR/Izm.

Serum calcium,albumin,globulin and matrix metalloproteinase-9 levels in acute cerebral infarction patients

BACKGROUND Hemorrhagic transformation(HT)is a common complication in patients with cerebral infarction.However,its pathogenesis is poorly understood.The knowledge of factors that may increase risk for HT may help in improving the safety of thrombolytic therapy.AIM To investigate the predictive value of serum calcium,albumin,globulin and matrix metalloproteinase-9(MMP-9)levels for HT after intravenous thrombolysis(IVT)in patients with acute cerebral infarction.METHODS Five hundred patients with acute cerebral infarction who received IVT with alteplase within 4.5 h after the onset of disease between January 2018 and January 2021 at our hospital were selected as the study subjects.They were divided into groups based on computed tomography scan results of the brain made within 36 h after thrombolysis.Forty patients with HT were enrolled in an observation group and 460 patients without HT were enrolled in a control group.Serum calcium,albumin,globulin and MMP-9 levels were compared between the two groups.Regression analysis was used to discuss the relationship between these indices and HT.RESULTS The previous history of hypertension,diabetes,atrial fibrillation,cerebrovascular diseases,smoking and alcohol intake were not associated with HT after IVT in patients with acute cerebral infarction(all P>0.05).The National Institutes of Health stroke scale(NHISS)score was associated with HT after IVT in patients with acute cerebral infarction(P<0.05).The serum calcium and albumin levels were lower in the observation group than in the control group(all P<0.05).The levels of globulin and MMP-9 were significantly higher in the observation group than in the control group(all P<0.05).Logistic regression analysis showed that NHISS score,serum calcium,albumin,globulins and MMP-9 were independent factors influencing the occurrence of HT following IVT in patients with cerebral infarction(P<0.05).CONCLUSION Serum calcium,albumin,globulin and MMP-9 levels are risk factors for HT after IVT in patients with acute cerebral infarction.Moreover,NHISS score can be used as a predictor of post-thrombolytic HT.

西门塔尔牛与荷斯坦牛产后血液离子和相关血气指标的比较分析

为分析西门塔尔牛产后血液离子和相关血气指标的变化特点,本试验连续收集西门塔尔头胎牛40头和经产牛60头作为试验组,连续收集荷斯坦头胎牛40头和经产牛60头作为对照组,比较2个组牛血液离子和相关血气指标的差异。结果显示,西门塔尔头胎牛和经产牛产后0 h亚临床低血钙发病率分别为7.5%和53.3%,总发病率为35.0%;西门塔尔牛产后0 h平均血液pH为7.42±0.01,平均血糖浓度为(6.91±0.16)mmol/L;西门塔尔头胎牛和经产牛初乳产量分别为(2.30±0.28)和(5.14±0.36)L,平均初乳产量为(4.01±0.28)L。荷斯坦头胎牛和经产牛产后0 h亚临床低血钙发病率分别为5.0%和61.7%,总发病率为39.0%;荷斯坦牛产后0 h平均血液pH为7.39±0.01,平均血糖浓度为(5.76±0.15)mmol/L;荷斯坦头胎牛和经产牛初乳产量分别为(3.68±0.36)和(5.96±0.33)L,平均初乳产量为(5.05±0.25)L。2个组牛产后0 h血钠、血钾、红细胞压积和血红蛋白水平组间和组内胎次间均无显著差异(P>0.05)。结果表明,西门塔尔头胎牛亚临床低血钙发病率高于荷斯坦头胎牛,可能与品种或饲料相关。而西门塔尔经产牛产后0 h血液离子钙水平高于荷斯坦经产牛,产后低血钙发病风险较低。西门塔尔牛维持产后血液pH和血液离子稳态的能力较强,较荷斯坦牛具有更好的抗分娩应激能力。本试验为制定西门塔尔牛围产期饲养管理和产后补钙策略提供了参考依据。

那屈肝素钙联合利伐沙班治疗肺动脉栓塞患者的临床效果

目的:探究那屈肝素钙联合利伐沙班治疗肺动脉栓塞(PE)患者的临床效果。方法:选取2022年3月—2024年3月六盘水市人民医院收治的90例PE患者。根据随机数表法将其分为对照组和观察组,各45例。两组均给予那屈肝素钙治疗,对照组给予华法林钠片,观察组给予利伐沙班片。比较两组治疗后临床疗效,治疗前后凝血功能、血气指标、心率、肺动脉收缩压(SPAP)及不良反应。结果:观察组治疗总有效率高于对照组,差异有统计学意义(P<0.05)。治疗后,观察组凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)均长于对照组,差异有统计学意义(P<0.05)。治疗后,观察组动脉血氧分压(PaO_(2))高于对照组,动脉血二氧化碳分压(PaCO_(2))、心率(HR)均低于对照组,差异有统计学意义(P<0.05)。治疗后,两组SPAP均较治疗前降低,观察组SPAP低于对照组,差异有统计学意义(P<0.05)。观察组不良反应发生率低于对照组,差异有统计学意义(P<0.05)。结论:那屈肝素钙联合利伐沙班治疗PE患者效果显著,可改善凝血功能,调节血气指标、心率,降低SPAP,且不良反应少。

Study on Effect of Gd (III) Speciation on Ca (II) Speciationin Human Blood Plasma by Computer Simulation

Ca (II) speciation and effect of Gd (III) speciation on Ca (II) speciation in human blood plasma were studied by computer simulation. [CaHCO3](-) is a predominant compound species of Ca (II). Gd (III) can compete with Ca (II) for biological molecules. The presence of Gd (III) results in a increase of concentration of free Ca (II) and a decrease of concentration of Ca (II) compounds.

阿托伐他汀钙联合依折麦布对冠心病的治疗效果与安全性研究

目的探讨临床对冠心病患者选择阿托伐他汀钙+依折麦布药物联合治疗后获得的临床效果,并观察对不良反应产生的影响。方法选取山东省第一康复医院2020年3月至2023年7月80例冠心病患者的临床治疗情况展开回顾性分析,依据不同用药方法完成治疗分组。单纯应用阿托伐他汀钙药物治疗的为对照组,共40例;在阿托伐他汀钙药物的基础上,添加依折麦布药物治疗的为研究组,共40例。对比两组的疗效、心功能指标、血脂指标以及不良反应。结果研究组冠心病患者治疗总有效率(97.50%)高于对照组(80.00%)(P<0.05)。治疗后,研究组冠心病患者除NT-ProBNP低于对照组之外,CO水平、SV水平、LVEF水平测定结果均高于对照组(均P<0.05)。治疗后,研究组冠心病患者除高密度脂蛋白胆固醇水平高于对照组之外,甘油三酯水平、总胆固醇水平、低密度脂蛋白胆固醇水平测定结果均低于对照组(均P<0.05)。研究组不良反应总发生率低于对照组(P<0.05)。结论临床对冠心病患者在治疗期间,阿托伐他汀钙+依折麦布药物的联合应用,可将疗效提升,将患者的血脂水平以及心功能双重改善,有效减少不良反应。

Simultaneous Biparametric Determination of Total Calcium and Potassium in Biological Fluids by Flow Injection Analysis──Use of Powell＇s Method in the System Optimization

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High-Level Circulating Total Calcium and Low Phosphate as Predictors of Insulin Resistance among Non-Diabetic Taxi-Motorbike Drivers Living and Working in Cotonou, Benin

Insulin resistance (IR) is a well-recognized marker of increased cardiovascular diseases (CVDs) and type 2 diabetes (T2D) risk. Therefore, screening for IR predictors would help reduce the likelihood of progression from early stage of IR to T2D or CVDs. However, the knowledge of association between IR and circulating total calcium (CTCa) and phosphate levels among non-diabetic patients in Benin is lacking. We investigated whether CTCa and phosphate levels within the normal ranges are associated with IR risk among taxi-motorbike drivers (TMDs) living and working in Cotonou. We evaluated 134 non-diabetic TMDs (aged 22 - 59 years) based on CTCa, phosphate, glucose, fasting insulin, and IR levels. IR was assessed using the homeostatic model assessment-insulin resistance (HOMA-IR). IR was defined as the 75th percentile of HOMA-IR value. Cardiometabolic factors were analyzed by tertiles of CTCa and phosphate levels (low, middle, and high groups). Logistic regression models evaluated the relationships between IR and CTCa and phosphate levels. Our results showed that participants with high CTCa levels had the highest prevalence of IR, elevated total cholesterol and high-density lipoprotein cholesterol. In a fully adjusted model, the odd ratio (OR) of having IR comparing the highest (>2.50 mmol/L) to the lowest CTCa levels (1.23 mmol/L) and the lowest levels (<1.10 mmol/L) of phosphate was 0.28 (p = 0.037). In conclusion, our study demonstrates that elevated CTCa and low phosphate levels are significant predictors of IR in non-diabetic patients. Continuous monitoring of these markers may help identify earlier individuals at greatest IR risk.

Effects of the mitochondrial calcium uniporter on cerebral edema in a rat model of cerebral ischemia reperfusion injury

The present study investigated the effects of the mitochondrial calcium uniporter inhibitor ruthenium red and the agonist spermine on cerebral edema in rats with cerebral ischemia reperfusion injury. Left middle cerebral artery occlusion （MCAO） was induced in rats using the suture method. Following 24 hours of ischemic reperfusion, neurological function scores of rats with MCAO, and rats pretreated with ruthenium red and spermine were significantly lower, however, water content of brain tissue, aquaporin 4 expression and immunoglobulin G （IgG） exudation were significantly higher than those of sham-operated rats. Compared with MCAO rats and spermine-treated rats, neurological function scores were considerably higher, and brain tissue water content, aquaporin 4 expression and IgG exudation decreased in ruthenium red-treated rats. These findings suggest that preventive application of the mitochondrial calcium uniporter inhibitor ruthenium red can significantly decrease aquaporin 4 and IgG expression, influence the permeability of the blood brain barrier, and thereby decrease the extent of cerebral edema.

Effects of Antihypertensive Drugs on Skin Blood Flow as an Indicator of Sympathetic Responses to Mental Stress in Hypertensive Patients

The aim of this study was to clarify the response of sympathetic activity to antihypertensive drugs using a mental stress test in hypertensive patients and to determine the effects of antihypertensive drugs on the sympathetic activitymediated hemodynamic response to mental stress. Hypertensive patients were divided into three groups according to the type of drug(s) being taken: a calcium antagonist group, an angiotensin II receptor blocker group, and a combination therapy group of calcium antagonists and angiotensin II receptor blockers. The Stroop color-word conflict test was applied as a mental stress test and hemodynamic responses to mental stress were measured, including blood pressure, pulse rate, and skin blood flow. Elevation of blood pressure by mental stress was suppressed in the combination therapy group compared with the calcium antagonist group. Reduction of skin blood flow by mental stress was suppressed in both the angiotensin II blocker group and the combination therapy group compared with the calcium antagonist group. In conclusion, skin blood flow can be a useful tool to evaluate sympathetic activity and combination therapy with calcium antagonists and angiotensin II receptor blockers were the most useful therapy for suppressing the hemodynamic response to mental stress.

丹参片联合那屈肝素钙注射液对髋部骨折术后下肢静脉血流参数的影响

目的探讨丹参片联合那屈肝素钙注射液对髋部骨折术后下肢静脉血流参数的影响。方法将2021年12月至2022年12月接受髋部骨折手术治疗的108例患者作为研究对象,按随机数字表法分为对照组和观察组,每组54例,2组均在术后开始用药,对照组采用那屈肝素钙注射液治疗,观察组采用丹参片联合那屈肝素钙注射液治疗,2组均治疗7 d后,对比术前及术后7 d患肢小腿直径差,对比术前及术后7 d疼痛分级(NRS)、下肢静脉血流参数(股总静脉、股浅静脉、股深静脉)、凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、骨碱性磷酸酶(BALP)、骨钙素(BGP)、Ⅰ型胶原羧基端交联肽(CTX-1),比较2组术后下肢静脉血栓发生率、药物不良发生率。结果观察组术前及术后7 d患肢小腿直径差明显低于对照组,NRS评分低于术前及对照组术后7 d,差异均有统计学意义(P<0.05);术后7 d观察组股总静脉、股浅静脉、股深静脉参数、TT、PT、APTT、BALP、BGP水平明显高于术前及对照组术后7天,CTX-1水平明显低于术前及对照组术后7天,差异均有统计学意义(P<0.05);2组不良反应总发生率比较,差异无统计学意义(P>0.05);观察组下肢脉血栓发生率明显低于对照组(P<0.05)。结论丹参片联合那屈肝素钙注射液用于髋部骨折术后,能缓解患者肿胀及疼痛,加快下肢静脉血流,降低患者血液高凝状态,改善骨代谢指标,进而降低血栓发生率。

药学服务对终末期肾脏病透析患者治疗效果影响的Meta分析

目的:通过Meta分析探讨药学服务对终末期肾脏病透析患者治疗效果的影响。方法:通过中国知网、万方、维普、PubMed和Web of Science等数据库收集有关药学服务对终末期肾脏病透析患者的治疗效果研究,使用RevMan5.3软件进行数据处理分析。结果:Meta分析最终纳入12篇文献,终末期肾脏病透析患者1614人,分析结果显示,药学服务组患者服药依从性较对照组明显提高(OR=8.37,95%CI[2.13,32.89],P=0.002);药学服务组患者血压(OR=4.77,95%CI[2.48,9.18],P<0.001)、血钙(OR=3.33,95%CI[1.77,6.30],P=0.0002)和血红蛋白(OR=4.39,95%CI[2.51,7.68],P<0.001)的达标率均显著大于对照组。结论:药学服务可提高终末期肾脏病透析患者服药依从性,并对血压、血钙和血红蛋白的改善起到积极作用。

基于倾向性评分匹配法分析术前血钙水平与老年心外科手术患者术后谵妄的关系

目的基于倾向性评分匹配法(PSM)分析术前血钙水平与老年心外科手术患者术后谵妄的关系。方法回顾性分析2020年5月至2023年10月德阳市人民医院心外科接受手术治疗的166例老年患者临床资料,依据术后住院期间是否发生谵妄将其分为发生组(52例)、未发生组(114例),采用PSM对两组患者临床资料进行匹配后得到组间协变量均衡的样本,单因素、logistic多因素回归分析法明确影响老年心外科手术患者术后谵妄的危险因素,受试者工作特征曲线(ROC)评估术前血钙水平预测老年心外科手术患者术后谵妄的效能。结果PSM后,共得到47对组间协变量均衡的样本。发生组、未发生组合并糖尿病比例、术后输注红细胞比例及白蛋白、血肌酐、血钙、神经元特异性烯醇酶(NSE)水平差异有统计学意义(P<0.05),合并高血压、高血脂比例、手术类型、血红蛋白、血小板计数等指标水平无明显差异(P>0.05)。Logistic多因素回归分析,结果显示,合并糖尿病(OR=1.790,95%CI 1.627~1.952)、白蛋白水平低(OR=1.804,95%CI 1.516~2.092)、血肌酐水平高(OR=1.844,95%CI 1.563~2.125)、血钙水平高(OR=1.835,95%CI 1.612~2.058)、NSE水平高(OR=1.840,95%CI 1.524~2.157)、术后输注红细胞(OR=1.808,95%CI 1.458~2.254)是影响老年心外科手术患者术后谵妄的危险因素,即上述指标与老年心外科手术患者术后谵妄有关。ROC曲线结果显示,术前血钙水平预测老年心外科手术患者术后谵妄的敏感度、特异度、AUC分别为87.50%、77.30%、0.906。结论老年心外科手术患者术后谵妄发生风险较高,术前血钙水平与老年心外科手术患者术后谵妄密切相关,且术前血钙水平升高可能是老年心外科手术患者术后谵妄发生的有效预测因子。

非布司他联合阿托伐他汀钙治疗高脂血症合并高尿酸血症患者的效果

目的:观察非布司他联合阿托伐他汀钙治疗高脂血症合并高尿酸血症患者的效果。方法:选取2020年2月至2023年3月该院收治的120例高脂血症合并高尿酸血症患者进行前瞻性研究,按照随机数字表法将其分为对照组和观察组各60例。对照组予以阿托伐他汀钙治疗,观察组在对照组基础上联合非布司他治疗。比较两组临床疗效、治疗前后血脂指标[总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]水平、血尿酸水平、血管内皮功能指标[可溶性细胞间黏附分子-1(sICAM-1)、内皮素-1(ET-1)]水平和不良反应发生率。结果:观察组治疗总有效率为93.33%(56/60),高于对照组的80.00%(48/60),差异有统计学意义(P<0.05);治疗后,观察组TG、TC、LDL-C、血尿酸水平均低于对照组,HDL-C水平高于对照组,差异有统计学意义(P<0.05);观察组sICAM-1、ET-1水平均低于对照组,差异有统计学意义(P<0.05);两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论:非布司他联合阿托伐他汀钙治疗高脂血症合并高尿酸血症患者可提高治疗总有效率,改善血脂指标水平,降低血尿酸和血管内皮功能指标水平,效果优于单纯阿托伐他汀钙治疗。

小剂量肝素钠与低分子肝素钙对脓毒症治疗作用的对比研究

目的:对比小剂量肝素钠与低分子肝素钙治疗脓毒症的效果。方法:选取2021年10月—2022年10月脓毒症患者100例,随机分为观察组和对照组,每组50例。对比两组患者的治疗效果。结果:对照组弥散性血管内凝血8例(16.0%),观察组弥散性血管内凝血1例(2.0%),观察组临床疗效高于对照组(P<0.05)。随访1个月,观察组病死率低于对照组(P<0.05)。观察组各项凝血指标显著优于对照组(P<0.05)。观察组各项炎性因子水平低于对照组(P<0.05)。两组治疗后急性生理与健康评分(APACHEⅡ)优于治疗前,且观察组优于对照组,差异有统计学意义(P<0.05)。结论:低分子肝素钙相比小剂量肝素钠治疗脓毒症更加可行,可降低并发症发生率,改善短期预后。

一次性口服葡萄糖酸钙在机采血小板献血者中的应用效果分析

目的:探讨一次性口服葡萄糖酸钙在机采血小板献血者中的应用效果。方法:选取2023年1月—2024年7月于山东省血液中心进行机采血小板的献血者100名作为研究样本,通过随机数字表法分为对照组(n=50)、观察组(n=50)。观察组献血者在机采血小板前一次性口服葡萄糖酸钙10 mL,对照组献血者在机采血小板过程中少量多次口服葡萄糖酸钙。比较两组献血者机采血小板前后血清甲状旁腺激素、钙离子、磷离子水平以及献血不良反应发生情况。结果:采血前,两组血清甲状旁腺激素、钙离子、磷离子水平比较,无统计学差异(P>0.05)。采血后,两组血清甲状旁腺激素水平升高(P<0.05),钙离子、磷离子水平变化不大(P>0.05);两组间血清甲状旁腺激素、钙离子、磷离子水平对比,无统计学差异(P>0.05)。观察组献血不良反应轻于对照组(P<0.05)。结论:机采血小板献血者采血前一次性口服葡萄糖酸钙有助于控制血清甲状旁腺激素水平大幅度波动,并且能够预防献血不良反应的发生。

蘑菇中毒导致心肌损伤的危险因素分析及预测模型构建与验证

目的 回顾性分析蘑菇中毒患者发生心肌损伤的危险因素并构建与验证预测模型。方法 将2012年7月至2023年9月湖南省人民医院和广西壮族自治区贵港市平南县人民医院收治的蘑菇中毒患者211例,根据中毒后有无心肌损伤情况分为两组,比较两组中毒患者的一般资料、心肌酶谱、心电图等情况,采用单因素和多因素回归分析蘑菇中毒患者发生心肌损伤的危险因素,并据此构建与验证危险因素预测模型。结果 单因素分析显示,心肌损伤组患者乳酸脱氢酶、肌酸激酶、肌酸激酶同工酶、肌红蛋白、肌钙蛋白Ⅰ、心电图异常均高于非心肌损伤组患者(P均<0.05),血红蛋白、血钙均低于非心肌损伤组患者(P均<0.05)。多因素回归分析结合ROC曲线分析表明,肌红蛋白、肌钙蛋白Ⅰ水平升高,血钙水平降低,以及心电图异常是蘑菇中毒导致心肌损伤的独立危险因素。通过Hosmer-Lemeshow检验,模型的内部一致性良好,拟合优度高(P>0.05)。结论 肌红蛋白、肌钙蛋白Ⅰ、血钙、心电图异常是预测蘑菇中毒导致心肌损伤的重要因素。在临床实践中,应重视高危因素的识别,监测相关指标,并及时采取干预治疗,以促进患者康复。

基于线粒体钙稳态探讨血府逐瘀汤对冠心病血瘀证模型大鼠的作用机制

目的基于线粒体钙稳态探讨血府逐瘀汤治疗冠心病血瘀证的作用机制。方法150只SD大鼠随机均分为正常组、假手术组(只穿线不结扎)、模型组、阿托伐他汀钙组(0.90 mg/kg·d,以下简称他汀组)以及血府逐瘀汤低、中、高剂量组[3.51、7.02、14.04 g/(kg·d),以下简称低、中、高剂量组],每组6只。除正常组和假手术组外,其他组均通过结扎大鼠冠状动脉左前降支制备冠心病血瘀证模型,造模成功24 h后灌胃给药,连续灌胃7 d,取各组大鼠左室缺血区心肌组织。HE染色观察样本坏死病变情况;线粒体钙离子探针检测线粒体内钙离子的荧光强度;定磷法检测心肌细胞Ca^(2+)-Mg^(2+)-ATP酶活性;免疫组织化学检测心肌组织线粒体钙单向转运蛋白(mitochondrial calcium uniporter,MCU)、心肌肌浆网钙离子ATP酶(sarco/sndoplasmic reticulum Ca^(2+)-ATPase 2a,SERCA2a)、瞬时受体电位通道3(transient receptor potential canonical 3,TRPC3)、钙释放激活钙通道1(calcium release-activated calcium channel protein 1,ORAI1)、半胱氨酸(cysteinyl aspartate specific proteinase,Caspase)-3、Caspase-9的蛋白表达。结果与正常组相比,模型组大鼠心肌组织可见细胞排列紊乱,横纹模糊,大量心肌细胞坏死,线粒体Ca^(2+)平均荧光强度、MCU、TRPC3、ORAI1、Caspase-3、Caspase-9的AOD值明显升高(P<0.01),Ca^(2+)-Mg^(2+)-ATP酶活性、SERCA2a的AOD值明显下降(P<0.01);与模型组相比,他汀组以及血府逐瘀汤各剂量组,心肌细胞水肿程度减轻,炎性细胞浸润减少,细胞排列相对紧密,细胞病理损伤程度有所缓解,他汀组以及血府逐瘀汤各剂量组的SERCA2a的AOD值显著上升(P<0.01),线粒体Ca^(2+)平均荧光强度、MCU、TRPC3、ORAI1、Caspase-9的AOD值显著下降(P<0.05,P<0.01),血府逐瘀汤各剂量组的Ca^(2+)-Mg^(2+)-ATP酶活性显著上升(P<0.05,P<0.01)以及Caspase-3的AOD值显著下降(P<0.05,P<0.01)。结论血府逐瘀汤可以调控冠心病血瘀证模型大鼠心肌线粒体钙离子转运,调节心肌细胞线粒体钙稳态,可能与上调SERCA2a的蛋白表达,下调MCU、TRPC3、ORAI1、Caspase-3、Caspase-9的蛋白表达相关。

80例中国东部沿海某医院食管癌患者代谢指标特点及临床意义

目的旨在探讨食管癌患者代谢指标的变化及其与食管癌发生的相关性。方法选取2020年1月—2023年6月青岛市黄岛区中心医院食管癌患者80例,与同期健康体检者81例,分别纳入癌症组和对照组,收集他们的人口学特征及临床资料,检测血清总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、高密度脂蛋白(high-density lipoprotein,HDL)、低密度脂蛋白(low-density lipoprotein,LDL)、脂蛋白a(lipoprotein-a,LP-a)、尿酸(uric acid,UA)、血钙(calcium,Ca)等代谢指标水平,对比上述指标在2组间的差异,探讨血脂代谢水平、UA、Ca与食管癌的相关性。结果癌症组与对照组体质量指数(BMI)、TC、TG、LDL、Ca无统计学差异,与癌症组比差异有统计学意义的是饮酒史、吸烟史、LP-a、HDL、UA(P<0.05)。二元Logistic回归分析发现,食管癌的发生与吸烟有很大的关系,同时发现LP-a升高和UA降低与食管癌的发生发展密切相关。结论食管癌患者存在明显血脂代谢异常,其中吸烟史和LP-a是食管癌的危险因素,UA为食管癌的保护因素。