Effect of Phytase Enzyme on Organs Growth Performance and Blood Profile of Broiler

Enzymes which are biological catalyst composed of amino acids with minerals and vitamins. The use of enzymes has many benefits in poultry diets include not only enhanced feed conversion and bird performance but also reduced output of excreta problems of environment. The present research work was conducted to evaluate the effect of phytase enzyme on growth performance and blood profile of broiler. Birds were arbitrarily separated into four groups, i.e. Group A (control) was offered 0 g/kg, 0.05 g/kg (group B), 0.075 g/kg (group C) and 0.025 g/kg (group D) phytase enzyme provided in broiler feed. Parameters which were selected in present research work as, feed intake, live body weight, feed conversion ratio, growth performance and blood profile. Results showed that the maximum chicken body weight was noted in group C and feed intake was minimum in group C, as compared to other groups. Feed conversion ratio was significantly (p < 0.05) higher in group C, followed by other groups the non-significantly variance founded respectively, the highest weight of liver, gizzard, heart, intestine were noted in treated group C comparatively, highest spleen weight was in group B and in group A (control) the minimum weight of spleen was noted and the average blood profile of broiler chicks in different groups, the white blood cells was significantly higher in group D followed by group A, group B and C. Red blood cells was significantly (P < 0.05) better in group C. Haemoglobin was significant differences in different groups of trial. A significant (P < 0.05) difference in Packed cells volume in group A followed by group B, C and D. Total protein in group A was minimum followed by group B, C and D, respectively. From the present study, it was concluded that supplementation of 0.075 g/kg phytase enzyme has a better effect on growth performance and blood profile of broiler.

Blood lipid profiles following nonfocused ultrasonic treatment for noninvasive body contouring

Aim:Blood lipid profiles changed following nonfocused ultrasound treatments for body contouring.The present study elucidates clinical effects of these devices on adipose tissue.Methods:Ultrasound treatments for 5 males and 5 females in a supine position,ages 37-67 years,were applied at 20 KHz and 3.0 W/cm2 in modulated emission.Whole abdomen was treated by an ultrasound handpiece for 30 min followed by a 6-min lymphatic drainage.Waist circumferences at the level of the umbilicus and body weight were measured before and immediately after treatment.Blood lipid profiles including total cholesterol,low-density lipoprotein,high-density lipoprotein,triglyceride(TG),nonesterified fatty acid(NEFA)and lipoprotein-a were measured at baseline,every 10 min during treatment,and 15,30,45,60,75,and 90 min after treatment completion.Results:NEFA showed statistically higher values after 10 min following treatment initiation.Subsequent values remained high despite some fluctuation,reaching a maximum at 90 min.In contrast,TG gradually decreased in concentration until the last measurement,especially for the first 30 min,with statistically significant reduction.Changes in other lipid profiles and lipoprotein-a were not significant.Conclusion:Changes in NEFA concentration were significant following ultrasound treatment,and suggest that metabolism of TGs stored within the adipocytes occurred immediately after treatment initiation.

Metabolomic Profiling Reveals Distinct Patterns of Tricarboxylic Acid Disorders in Blood Stasis Syndrome Associated with Coronary Heart Disease

Objective： To investigate the underlying metabolomic profiling of coronary heart disease（CHD） with blood stasis syndrome（BSS）. Methods： CHD model was induced by a nameroid constrictor in Chinese miniature swine. Fifteen miniature swine were randomly divided into a model group（n=9） and a control group（n=6）, respectively according to arandom number table. After 4 weeks, plasma hemorheology was detected by automatic hemorheological analyzer, indices including hematocrit, plasma viscosity, blood viscosity, rigidity index and erythrocyte sedimentation rate; cardiac function was assessed by echocardiograph to detect left ventricular end-systolic diameter（LVED）, left ventricular end-diastolic diameter（LVEDd）, ejection fraction（EF）, fractional shortening（FS） and other indicators. Gas chromatography coupled with mass spectrometry（GC-MS） and bioinformatics were applied to analyze spectra of CHD plasma with BSS. Results： The results of hemorheology analysis showed significant changes in viscosity, with low shear whole blood viscosity being lower and plasma viscosity higher in the model group compared with the control group. Moreover, whole blood reduction viscosity at high shear rate and whole blood reduction viscosity at low shear rate increased significantly（P〈0.05）. The echocardiograph results demonstrated that cardiac EF and FS showed significant difference（P〈0.05）, with EF values being decreased to 50% or less. The GC-MS data showed that principal component analysis can clearly separate the animals with BSS from those in the control group. The enriched Kyoto Encyclopedia of Genes and Genomes biological pathways results suggested that the patterns involved were associated with dysfunction of energy metabolism including glucose and lipid disorders, especially in glycolysis/gluconeogenesis, galactose metabolism and adenosine-triphosphate-binding cassette transporters. Conclusion： Glucose metabolism and lipid metabolism disorders were the major contributors to the syndrome classification of CHD with BSS.