Study on Polymorphisms in the Blood Protein of Tibetan Mastiff

[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus（Tf, Po, Sα2, Hb, AIb, Pr and Amy）were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei＇s average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.

Long-term effects of early antibiotic intervention on blood parameters,apparent nutrient digestibility, and fecal microbial fermentation profile in pigs with different dietary protein levels

Backgroud： This study aimed to determine the effects of early antibiotic intervention（EAI） on subsequent blood parameters, apparent nutrient digestibility, and fecal fermentation profile in pigs with different dietary crude protein（CP） levels. Eighteen litters of piglets（total 212） were randomly allocated to 2 groups and were fed a creep feed diet with or without in-feed antibiotics（olaquindox, oxytetracycline calcium and kitasamycin） from postnatal d 7 to d 42. On d 42, the piglets within the control or antibiotic group were mixed, respectively, and then further randomly assigned to a normal-（20%, 18%, and 14% CP from d 42 to d 77, d 77 to d 120, and d 120 to d 185,respectively） or a low-CP diet（16%, 14%, and 10% CP from d 42 to d 77, d 77 to d 120, and d 120 to d 185,respectively）, generating 4 groups. On d 77（short-term） and d 185（long-term）, serum and fecal samples were obtained for blood parameters, microbial composition and microbial metabolism analysis.Results： EAI increased（P 〈 0.05） albumin and glucose concentrations in low-CP diet on d 77, and increased（P 〈 0.05） urea concentration in normal-CP diet. On d 185, EAI increased（P 〈 0.05） globulin concentration in normal-CP diets, but decreased glucose concentration. For nutrient digestibility, EAI increased（P 〈 0.05）digestibility of CP on d 77. For fecal microbiota, the EAI as well as low-CP diet decreased（P 〈 0.05） E. coli count on d 77. For fecal metabolites, on d 77, EAI decreased（P 〈 0.05） total amines concentration but increased skatole concentration in low-CP diet. On d 185, the EAI increased（P 〈 0.05） putrescine and total amines concentrations in low-CP diets but reduced（P 〈 0.05） in the normal-CP diets. The low-CP diet decreased the concentrations of these compounds.Conclusions： Collectively, these results indicate that EAI has short-term effects on the blood parameters and fecal microbial fermentation profile. The effects of EAI varied between CP levels, which was characterized by the significant alteration of glucose and putrescine concentration.

Productive performance and blood metabolites as affected by protected protein in sheep

This investigation included two experiments. Experiment 1 was executed to study the effect of feeding different rations of protected protein of canola meal on digestibility and nutritive values within sheep. Twenty male, healthy sheep were divided into five treatments according to the methods of protein protection (control, heat, sodium hydroxide, formaldehyde, and acetic acid treatments). Experiment 2 was carried out on developing lambs to investigate the effect of protected protein on growth performance and some blood metabolites. Animals in this ex-periment were also divided into the same treatments as Experiment 1. Animals in the first and second experiment were fed concentrate ration (80%) and wheat straw (20%) to cover the feed requirements. Nutritive values expressed as total digestible nutrients (TDN %) and digestible crude protein (DCP%) of the experimental rations was calculated. In the second experimental all animals were weighed biweekly and the amounts of rations were adjusted throughout the experimental period (120 days) according to their body weight change. Results indicated that in the first experimental protected protein by heat (HE) and sodium hydroxide (NH) had positive (P<0.05) effects on most of digestibility coefficients of different nutrients. Protein protection methods also improved (P<0.05) the nutritive values (TDN and DCP) in the HE treatment and NH treatment. In the second experiment body weight increased by 14% and 7% and also daily gain by 27% and14 % in HE and NH, respectively, while FM and AC decreased body weight by 8% and 4.4%. Higher values (P<0.01) in both thyroid hormones were observed in HE and NH than those other treatments. Also, higher values (P<0.01) of total protein, albumin, and glucose were observed in HE and NH than other treatments. The control (CTL) group recorded higher concentrations of urea-N and creatinine at different periods of the experiment in comparison with other treatments. Generally, from the present investigation it can be concluded that protected protein of canola meal by heat or sodium hydroxide treatments were more efficient for productive performance and some blood metabolites of sheep.

Correlation between intakes of carbohydrates,protein,and fat with random blood sugar levels in menopausal women

Objective: Carbohydrates, proteins, and fats are energy sources needed by the body for performing daily activities and generating primary energy substances. In women who have undergone menopause, the function of thyroid hormone in their body begins to decline and thus affects the ability of the body to produce energy. The purpose of this study was to determine the correlation between the intakes of carbohydrates, proteins, and fats and random blood sugar levels in menopausal women.Methods: This study was a correlational analytical research with a cross-sectional design, which was conducted in 72 menopausal women recruited by the purposive sampling technique. In this study, a 24-hour food recall form was used, and randomized blood sugar levels were measured using a glucometer. The analysis of the data was performed using a Pearson product moment and multiple linear regression.Results: Carbohydrates and fats together affected random blood sugar levels with an F-value of 25.810 and a p-value of 0.000.Meanwhile, adjusted R^2 showed the value of 0.411, indicating that the difference in the intake of carbohydrates and fats together affected random blood sugar levels by 41.1%.Conclusions: Intake of carbohydrates and fats affected random blood sugar levels, whereas the rest were influenced by other factors,and protein intake was unrelated to random blood sugar levels in menopausal women.

Comparison of the level of thrombus precursor protein in blood plasma between patients with acute cerebral infarction and healthy persons at different time

BACKGROUND： Thrombus precursor protein （TpP） is the index of thrombus activity level, and it is also early referencing index in detecting thrombus diseases. OBJECTIVE： To dynamically observe the changes of TpP level in blood plasma of patients with acute cerebral infarction at different time after onset, and to compare the differences of plasma TpP level between patients with acute cerebral infarction and healthy persons who received health examination. DESIGN： Controlled observation SETTING： Department of Neurology, Affiliated Hospital of Xuzhou Medical College PARTICIPANTS： Totally 58 patients with acute cerebral infarction who received the treatment in the Department of Neurology, Affiliated Hospital of Xuzhou Medical College between September 2004 and March 2005 were recruited in this study. They all met the diagnostic criteria revised by the 4^th National Conference of Cerebrovascular Disorders in 1995 and were diagnosed by clinical and skull CT and （or） MRI examinations. The patients included 33 male and 25 female aged from 36 to 87 years. Time to onset 〈 6 hours, 6 to 11 hours, 12 to 23 hours, 24 to 48 hours and 〉 48 hours were found in 10,11,14,10 and 13 patients respectively. Another 51 persons who homeochronously received the health body examination in our hospital were recruited, including 34 male and 17 female, aged 38 to 85 years, serving as control group. Patients with cardio-cerebrovascualr diseases or liver and kidney diseases were excluded. All the involved subjects were informed of the detected items. METHODS： About 4 mL venous blood was respectively taken from patients admitted to the hospital within 6 hours, 6 toll hours, 12 to 23 hours, 24 to 48 hours and more then 48 hours after onset, and healthy persons when receiving health examination. The level of TpP in blood plasma was measured with enzymelinked immunosorbent assay. MAIN OUTCOME MEASURES： ① Comparison of the level of plasma TpP between patients and controls;② Comparison of the level of plasma TpP of patients with acute cerebral infarction at different time after onset. RESULTS： Totally 58 patients with acute cerebral infarction and 51 persons who received health examination participated in the result analysis. ①Comparison of plasma TpP level between patients and controls： The plasma TpP level of patients with acute cerebral infarction was significantly higher than that of control group [（16.12±3.28）vs （5.38±1.36） mg/L, t= 20.993, P〈 0.01 ]. ② Comparison of plasma TpP level of patients with acute cerebral infarction at different time after onset： The level of plasma TpP was （12.06±3.06） mg/L within 6 hours, （15.11±3.42） mg/L at 6 to 11 hours, （20.63±4.05） mg/L at 12 to 23 hours, （16.15±3.50） mg/L at 24 to 48 hours and （11.88±3.11） mg/L at more than 48 hours after onset. It increased from the 6^th hour, reached the peak at the 12^th to 23^rd hours, maintained at very high level at the 48= hour and then gradually decreased and recovered to the level within 6 hours after onset. The level of plasma TpP of patients with acute cerebral infarction was signiticantly higher at the 12^th to 23^rd hours after onset and the 24^th to 48^th hours after onset than within 6 hours after onset （t = 13.385, P 〈 0.05）. CONCLUSION： ①The level of plasma TpP of patients with acute cerebral infarction is significantly higher than that of persons who received health examination.② Plasma TpP levels of patients with acute cerebral infarction change in wave manner at the different time after onset.

Protective role of buffalo pineal proteins on arsenic-induced oxidative stress in blood and kidney of rats

Objective: Exposure to various toxic metals has become an increasingly recognized source of ill- ness in human and animals, worldwide. Arsenic (As) and its compounds cause adverse health effects in animals and humans. Recently, it has been suggested that the pineal gland may also have antioxidants role due to secretary product other than melatonin. With keeping this view, pre-sent investigation tested effect of buffalo (Bubalus bubalis) pineal proteins (PP) on arsenic-induced oxidative stress in RBCs (Red blood cells) and kidney of rats. Methods: Eighteen adult female Wistar rats were grouped into group-I (Control), group-II (Arsenic control), and group-III (Arsenic + Pineal proteins). Experimental rats were given 100 ppm arsenic (p.o.) for 4 weeks alone or along with pineal proteins at a dose of 100 μg/kg body weight (i.p.). Results: Interestingly, arsenic ex-posure led to the stimulation of kidney catalase (CAT) activity, but inhibition of RBCs CAT activ-ity and significantly (P<0.05) increased the RBCs and kidney lipid peroxidation level (LPO). How-ever, arsenic treatment caused depletion of glu- tathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) in kidney tissues. In RBCs, only GR and CAT activity were significantly (P<0.05) declined. These changes were significantly (P<0.05) reversed by PP treatment in arsenic exposed animals. Conclusion: Therefore, present study indicated the significant protecting effect of buf-falo (Bubalus bubalis) PP against arsenic in-duced-oxidative stress through antioxidant de-fense systems in rats.

Monitoring the changes in plasm C-reactive protein,fibrinogen and blood white cell in patients with primary hypertension combined with acute cerebral infarction

BACKGROUND： Inflammatory reaction and the increased level of its accompanying active protein play an important role in the occurrence and development of cerebral infarction. C-reactive protein, fibrinogen and white blood cell, as the monitoring index of inflammatory reaction, are very important in the occurrence and development of acute cerebral infarction. OBJECTIVE： To make a comparison between patients with primary hypertension accompanied with acute cerebral infarction and with simple primary hypertension by observing the changes in plasma C-reactive protein and fibrinogen levels as well as white blood cell and differential counts and analyzing their significances. DESIGN ： Controlled observation SETTING ： Ward Building for VIP, Shenzhen Hospital, Peking University. PARTICIPANTS： Totally 133 patients with primary hypertension were selected from Ward Building for VIP, Shenzhen Hospital, Peking University during September 2003 to September 2005, The diagnostic criteria were based on the hypertension diagnosis criteria formulated by the 7^th World Health Organization-International Society of Hypertension Guidelines （WHO-ISH） in 1998. The informed consents were obtained from all the participants. The involved patients were assigned into two groups： primary hypertension group, in which, there were 65 patients with primary hypertension （ degree 2）, including 42 males and 23 females, with mean age of （61 ±14）years and mean blood pressure of （162.7±6.8）/（94.2±8.4） mm Hg （1 mm Hg =0.133 kPa）, and primary hypertension combined with cerebral infarction group, in which, there were 68 patients with primary hypertension combined with cerebral infarction （ meeting the diagnostic criteria formulated in the 4^th National Cerebrovascular Diseases Meeting in 1995 and diagnosed by skull CT or MRI to exclude the patients with lacunar infarction）, including 42 males and 26 females, with mean age of （56±15） years and mean blood pressure of （176.4±9.2）/（96.3±9.7） mm Hg. METHODS： Plasm C-reactive protein and fibrinogen levels, and white blood cell and differential counts of patients in the two groups were examined 24 hours after stroke. The above indexes were re-examined in the primary hypertension combined with cerebral infarction group 72 hours after stroke. White blood cell and differential counts were performed with laser method （East Asia FE-95001 RAM-1, Japan）. The level of C-reactive protein was measured with turbidimetry （BNII Automatic Systems For Analysis, USA）. The level of fibrinogen was measured with algorithm method when prothrombin time was normal and with Clauss method when prothrombin time was abnormal （ACL Automatic Coagulation Analyzer, USA）. MAIN OUTCOME MEASURES： The plasm C-reactive protein and flbrinogen levels, and white blood cell and differential counts 24 hours after stroke in two groups and 72 hours after stroke in primary hypertension combined with cerebral infarction group. RESULTS： All the 133 involved patients participated in the result analysis. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neutrophil counts in patients with primary hypertension were all within the normal range. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neu- trophil counts in patients with primary hypertension combined with cerebral infarction were significantly higher than those in patients with primary hypertension 24 hours after stroke and 72 hours after stroke respectively[24 hours after stroke：（32.12±11.76） mg/L vs. （5.02±3.21 ） mg/L;（4.64±0.75） g/L vs. （3.12±0.49） g/L; （9.32±81）×10^9 L^- 1 vs. （5.78±1.32）×10^9L^- 1 （7.85±2.38）×10^9 L^- 1 vs.（3.49±1.28）×10^9 L^-1,t =7.094, 5.759,4.106,5.491, respectively,all P〈 0.01; 72 hours after stroke： （47.62±18.43） mg/L vs. （32.12±11.76） mg/L; （5.08±0.82） g/L vs. （4.64±0.75） g/L, t =2.864,2.220, respectively, both P 〈 0.05]. CONCLUSION： The increase in fibrinogen level and white blood cell count are the important index in monitoring primary hypertension combined with acute cerebral infarction. The increase in plasm C-reactive protein and fibrinogen levels 72 hours after stroke indicates that plasma C-reactive protein and fibrinogen are very important in the development of disease.

Transactivating-transduction protein-polyethylene glycol modified liposomes traverse the blood-spinal cord and blood-brain barriers

Naive liposomes can cross the blood-brain barrier and blood-spinal cord barrier in small amounts. Liposomes modified by a transactivating-transduction protein can deliver antibiotics for the treatment of acute bacterial infection-induced brain inflammation. Liposomes conjugated with polyethylene glycol have the capability of long-term circulation. In this study we prepared transactivating-transduction protein-polyethylene glycol-modified liposomes labeled with fiuorescein isothiocyanate. Thus, liposomes were characterized by transmembrane, long-term circulation and fluorescence tracing. Uptake, cytotoxicity, and the ability of traversing blood-spinal cord and blood-brain barriers were observed following coculture with human breast adenocarcinoma cells （MCF-7）. Results demonstrated that the liposomes had good biocompatibility, and low cytotoxicity when cocultured with human breast adenocarcinoma cells. Liposomes could traverse cell membranes and entered the central nervous system and neurocytes through the blood-spinal cord and blood-brain barriers of rats via the systemic circulation. These results verified that fluorescein isothiocyanate-modified transactivating-transduction protein-polyethylene glycol liposomes have the ability to traverse the blood-spinal cord and blood-brain barriers.

脓毒症患者白细胞计数、血清C反应蛋白、肝素结合蛋白、降钙素原表达及与病情进展及预后关系

目的探讨白细胞计数(WBC)、血清C反应蛋白(CRP)、肝素结合蛋白(HBP)和降钙素原在脓毒症患者病情进展和预后评估中的应用价值。方法将2018年4月至2023年3月内江市第一人民医院收治的205例脓毒症患者纳入本次回顾性研究,根据病情严重程度分为脓毒症组(n=129)和脓毒性休克组(n=76),并根据患者预后情况分成存活组(n=154)和死亡组(n=51)。检测脓毒症组与脓毒性休克组、存活组与死亡组患者的WBC、血清CRP、HBP、降钙素原水平,收集患者的急性生理与慢性健康状况评分系统Ⅱ(APACHEⅡ)评分和序贯器官衰竭评估(SOFA)评分,分析WBC、血清CRP、HBP、降钙素原与APACHEⅡ、SOFA评分的相关性;采用受试者工作特征(ROC)曲线分析上述4个指标单独及联合检测评估脓毒症患者预后。结果脓毒性休克组的WBC、血清CRP、HBP、降钙素原分别为(19.83±3.09)×10^(9)/L、(114.10±35.17)mg/L、(78.92±13.14)μg/L和(11.13±0.91)μg/L,均显著高于脓毒症组[(9.55±2.87)×10^(9)/L、(59.96±23.45)mg/L、(36.47±12.83)μg/L和(8.21±0.82)μg/L],差异均有统计学意义(P<0.05)。脓毒性休克组的APACHEⅡ评分、SOFA评分分别为(20.05±2.39)、(10.29±2.51)分,均显著高于脓毒症组[(16.21±2.30)、(7.90±2.17)分],差异均有统计学意义(P<0.05)。Pearson相关性分析显示,WBC、血清CRP、HBP、降钙素原与APACHEⅡ评分(r=0.554、0.593、0.713、0.651,P<0.05)、SOFA评分均呈显著正相关(r=0.540、0.571、0.687、0.609,P<0.05)。死亡组的WBC、血清CRP、HBP、降钙素原、APACHEⅡ评分、SOFA评分也均显著高于存活组,差异均有统计学意义(P<0.05)。ROC曲线分析显示,WBC、血清CRP、HBP、降钙素原及4个指标联合检测预测脓毒症患者不良预后的ROC曲线下面积分别为0.835、0.803、0.881、0.817和0.939。结论脓毒性休克患者的WBC、血清CRP、HBP、降钙素原显著高于脓毒症患者,可反映脓毒症患者的病情严重程度,4个指标联合检测对脓毒症患者的预后有较好的预测价值。

脂必泰联合阿托伐他汀钙对脑梗死患者颈动脉粥样硬化斑块临床研究

目的 探讨脂必泰胶囊对脑梗死急性期患者颈动脉粥样硬化斑块的影响。方法 选取90例脑梗死急性期患者,采用随机数字表法将患者分为治疗组和对照组各45例。对照组给予西医常规治疗(阿托伐他汀钙片),治疗组联合脂必泰胶囊强化降脂治疗,疗程3个月。比较两组患者治疗前后血清超敏C反应蛋白(hypersensitive C-reactive protein, hs-CRP)、血脂水平、颈动脉斑块积分、斑块面积及中医证候评分。结果 治疗后,两组患者血清hs-CRP、总胆固醇(total cholesterol, TC)、甘油三酯(triglyceride, TG)、低密度脂蛋白水平(low densith lipoprotein, LDL-C)、颈动脉硬化斑块积分、斑块面积、中医证候评分均明显低于治疗前(P<0.05);且治疗组明显低于对照组,差异有统计学意义(P<0.05)。结论 脂必泰胶囊联合阿托伐他汀钙能够明显降低脑梗死急性期患者血脂和hs-CRP水平,减轻颈动脉粥样斑块,改善中医临床证候。

低蛋白质氨基酸平衡饲粮添加谷氨酸对断奶仔猪生长性能、血液抗氧化和免疫指标的影响

试验旨在研究较大幅度降低饲粮粗蛋白质(CP)水平,平衡必需氨基酸并添加谷氨酸对断奶仔猪生长性能、血液抗氧化和免疫指标的影响。试验选用144头杜长大杂交仔猪,随机分成3组,每组6个重复,每个重复8头猪,试验期16 d。Ⅰ组(对照组)饲粮CP水平为21.16%,Ⅱ组饲粮CP水平为15.97%,补充必需氨基酸(EAA)赖氨酸(Lys)、蛋氨酸(Met)、苏氨酸(Thr)、色氨酸(Trp)、缬氨酸(Val)和异亮氨酸(Ile),Ⅲ组为Ⅱ组+谷氨酸(Glu)。结果表明,仔猪平均日增重(ADG)Ⅱ组显著低于Ⅰ组和Ⅲ组(P<0.05)。Ⅱ组仔猪腹泻率显著低于Ⅰ组(P<0.05),Ⅲ组显著低于Ⅱ组(P<0.05),Ⅲ组极显著低于Ⅰ组(P<0.01)。Ⅲ组血浆总抗氧化能力(T-AOC)显著高于Ⅰ组和Ⅱ组(P<0.05)。血浆免疫球蛋白G(IgG)、免疫球蛋白A(IgA)、免疫球蛋白M(IgM)和白细胞介素-10(IL-10)含量Ⅲ组显著高于Ⅰ组和Ⅱ组(P<0.05),Ⅱ组血浆IL-10含量显著低于Ⅰ组(P<0.05)。综上,断奶后饲喂低蛋白质氨基酸平衡并添加谷氨酸的饲粮能够提高断奶仔猪生长性能、减少腹泻、增强机体抗氧化和免疫功能。

肝癌患者外周血中抑制树突状细胞分化的蛋白成分筛选及鉴定

目的肝癌患者外周血中靶向抑制树突状细胞分化成熟的蛋白成分筛选及鉴定。方法利用免疫磁珠分选健康人外周血CD14^(+)单核细胞,诱导分化为未成熟的树突状细胞(iDCs),进一步诱导分化为成熟的树突状细胞(mDCs),鉴定iDCs纯度。酶切健康人和肝癌患者血清孵育的iDCs的结合蛋白,回收酶切后的多肽,筛选对iDCs分化成熟有潜在调控作用的互作候选蛋白。靶向鉴定差异候选蛋白。构建过表达蛋白质粒,转染至正常肝细胞(HL7702),将细胞培养上清液与iDCs共同培养,诱导iDCs成熟,对特异性抑制iDCs成熟的肝癌源性血清蛋白进行特异性筛选和鉴定。结果TNF-α诱导前抗原标志物阳性细胞表达CD86^(+)比例为79.2%、黏附分子CD40^(+)比例为83.3%、MHC-Ⅱ抗原分子HLA-DR^(+)比例为67.6%、CD83^(+)比例为5.5%以及CD1a^(+)比例为13.7%,符合iDCs的抗原表型特征;TNF-α诱导后抗原标志物阳性细胞表达CD86^(+)比例为92.0%、CD40^(+)比例为89.0%、HLA-DR^(+)比例为68.3%、CD83^(+)比例为37%、CD1a^(+)比例为48.3%,提示iDCs已迅速分化成熟为mDCs,符合mDCs的抗原表型特征。初步确认40多种对iDCs分化成熟有潜在调控作用的互作候选蛋白。选择10种差异候选蛋白,对其过表达进行验证,显示AFP蛋白、AGXT蛋白、ATP1A1蛋白过表达能够明显抑制iDCs分化成熟为mDCs。结论共筛选出10种靶向抑制树突状细胞分化的肝癌外周血清蛋白,其中AFP蛋白、AGXT蛋白、ATP1A1蛋白能够显著抑制树突状细胞分化成熟。

结直肠癌患者术前外周血淋巴细胞/C反应蛋白比值的临床意义研究

研究淋巴细胞计数/C-反应蛋白比值[Lymphocyte(/μL)/C-reactive protein(mg/dL)ratio,LCR]对结直肠癌(col-orectal cancer,CRC)根治术患者的临床意义。以2015年1月—2016年6月寿光市人民医院收治的CRC根治术患者为对象行回顾性研究,比较炎症标志物对CRC术后并发症和患者生存期的预测价值。研究结果显示,淋巴细胞计数/CRP比率(lymphocyte count/CRP ratio,LCR)预测术后并发症的曲线下面积为0.769。LCR≤12450是术后并发症的独立危险因素;年龄>65岁、LCR≤12450、CRP/白蛋白比率(CRP/albumin ratio,CAR)>0.27是总生存期(overall survival,OS)和无复发生存期(recurrence-free survival,RFS)较差的独立风险因素;N分期阳性是RFS较差的独立风险因素。研究发现,术前外周血LCR是预测CRC根治术后并发症、OS和RFS的良好指标。

慢性乙型肝炎者外周血SAA/CRP、NLR水平与HBV-DNA载量、病情程度的相关性分析

目的探究慢性乙型肝炎(chronic hepatitis B,CHB)患者外周血淀粉样蛋白A(serum amyloid A,SAA)与C反应蛋白(C-reactive protein,CRP)比值(SAA/CRP)、中性粒细胞与淋巴细胞比值(neutrophils lymphocytes ratio,NLR)水平与乙型肝炎病毒-脱氧核糖核酸(HBV-DNA)载量及病情程度的相关性。方法选取2020年6月至2022年6月达州市中西医结合医院100例CHB患者作为研究组,根据病情程度分为轻度(单纯CHB,n=36)、中度(乙肝代偿期肝硬化,n=33)和重度(乙肝失代偿期肝硬化,n=31)。另选同期、同年龄段50例健康志愿者作为对照组,比较研究组不同病情程度、对照组一般资料、血清SAA/CRP、NLR水平,并比较研究组不同HBV-DNA载量患者血清SAA/CRP、NLR水平,分析CHB患者血清SAA/CRP、NLR水平与HBV-DNA载量、病情程度的相关性;所有患者均行抗病毒治疗,治疗24周,比较不同抗病毒疗效患者治疗前、治疗后12周、24周血清SAA/CRP、NLR水平及变化值,分析治疗前后血清SAA/CRP、NLR水平变化值预测疗效的价值。结果重度CHB患者血清SAA/CRP、NLR水平>中度CHB患者>轻度CHB患者>健康人群(P<0.05);高载量患者血清SAA/CRP、NLR水平>中载量患者>轻载量患者(P<0.05);CHB患者血清SAA/CRP、NLR水平与HBV-DNA载量(r=0.756、0.709)、病情程度(r=0.776、0.745)呈正相关(P<0.05);无应答患者治疗后12周、24周外周血SAA/CRP、NLR水平均高于应答患者,变化值均低于应答患者(P<0.05);SAA/CRP△1、NLR△1单独预测的AUC分别为0.752、0.773,联合预测△1的AUC为0.861;SAA/CRP△2、NLR△2单独预测的AUC分别为0.796、0.819,联合预测△2的AUC为0.967,大于联合预测△1的AUC(P<0.05)。结论CHB患者的SAA/CRP、NLR与CHB HBV-DNA载量及病情程度具有相关性,临床可通过其水平变化评估病情及预测预后。

甲磺酸阿帕替尼联合多西他赛二线治疗晚期胃癌的效果及对外周血肿瘤异常蛋白、血管内皮生长因子与基质金属蛋白酶-9的影响

目的探讨甲磺酸阿帕替尼联合多西他赛二线治疗晚期胃癌的效果及对外周血肿瘤异常蛋白、血管内皮生长因子(VEGF)与基质金属蛋白酶-9(MMP-9)的影响。方法选取2020年3月至2022年3月收治的90例晚期胃癌患者为研究对象,随机将其分为对照组与观察组,各45例。对照组采用多西他赛化疗方案,观察组在对照组基础上加甲磺酸阿帕替尼治疗。比较两组的治疗效果。结果观察组的疾病控制率高于对照组(P<0.05)。治疗后,观察组的糖类抗原19-9(CA19-9)、肿瘤特异性生长因子(TSGF)及癌胚抗原(CEA)水平均低于对照组(P<0.05)。治疗后,观察组的VEGF、MMP-9、肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)水平均低于对照组(P<0.05)。两组的不良反应总发生率比较,差异无统计学意义(P>0.05)。结论甲磺酸阿帕替尼联合多西他赛二线治疗晚期胃癌的效果较好,可对外周血肿瘤异常蛋白有良好的抑制作用,同时还能调节VEGF、MMP-9水平,且不会增加不良反应发生风险,值得应用与推广。

维生素D及二甲双胍用于妊娠期糖尿病患者的疗效及对妊娠结局和视黄醇结合蛋白4的影响

目的分析维生素D与二甲双胍对妊娠期糖尿病患者的治疗效果。方法筛选2023年3月至2024年3月收治的200例妊娠期糖尿病患者,按照随机数字表法分为对照组和观察组,每组100例。对照组接受二甲双胍治疗,观察组在对照组基础上配合维生素D治疗。比较2组糖代谢指标、肾功能、血清因子[肿瘤坏死因子-α(TNF-α)、视黄醇结合蛋白4(RBP4)、内脂素(visfatin)]及血脂指标[三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)],观察2组妊娠结局及不良反应。结果观察组治疗后空腹血糖、餐后2 h血糖、糖化血红蛋白、稳态模型胰岛素抵抗指数、TNF-α、RBP4、visfatin、TC、TG、LDL-C低于对照组,空腹胰岛素高于对照组(P<0.05)。治疗后,2组血肌酐、24 h尿蛋白总量及血尿素低于治疗前,且观察组低于对照组(P<0.05)。观察组巨大儿、产后出血、新生儿窒息发生率以及不良反应总发生率均低于对照组(P<0.05)。结论妊娠期糖尿病应用维生素D与二甲双胍共同治疗的效果更好,可维持糖代谢和血脂水平,调节肾功能,稳定RBP4水平,从而改善妊娠结局,并控制不良反应。

HBGH患者血清CGN、SDC-1水平与病情及疾病转归的关系

目的 探讨血清扣带蛋白(CGN)、多配体聚糖-1(SDC-1)水平与高血压性基底节脑出血(HBGH)患者病情及疾病转归的关系。方法 选取2019年2月至2022年2月连云港市第二人民医院(下称该院)收治的123例HBGH患者为研究对象,另选择该院同期行健康体检的体检健康者120例为健康组。检测两组纳入对象血清CGN、SDC-1表达水平。根据疾病转归情况将患者分为好转组92例,恶化组31例。采用受试者工作特征(ROC)曲线及曲线下面积(AUC)分析血清CGN、SDC-1表达水平对HBGH患者疾病转归的预测价值。结果 重度组血清CGN、SDC-1表达水平均高于中度组、轻度组,且中度组血清CGN、SDC-1表达水平均高于轻度组,差异有统计学意义(P<0.05),3组HBGH患者血清CGN、SDC-1表达水平均高于健康组,差异有统计学意义(P<0.05)。恶化组血清CGN、SDC-1表达水平高于好转组,差异有统计学意义(P<0.05)。血清CGN、SDC-1预测HBGH患者疾病转归的AUC分别为0.742(95%CI:0.792~0.697)、0.861(95%CI:0.906~0.910),两者联合预测的AUC为0.917(95%CI:0.962~0.870)。恶化组出血量、破入脑室情况明显高于好转组,入院格拉斯哥昏迷量表(GCS)评分低于好转组,差异有统计学意义(P<0.05)。多因素Logistic回归分析显示,血清CGN≥51.63 pg/mL(OR=3.815)、血清SDC-1≥450.67μg/L(OR=4.230)、入院GCS评分≤8分(OR=5.333)是HBGH患者疾病转归的影响因素(P<0.05)。结论 血清CGN、SDC-1表达水平升高与HBGH患者病情加重、疾病转归恶化密切相关,二者对HBGH患者疾病转归具有一定预测价值。

肝素结合蛋白、总胆红素和白细胞计数联合预测严重创伤合并脓毒症的效能评价

目的:评价肝素结合蛋白(HBP)联合脏器功能指标对严重创伤合并脓毒症患者预警诊断和预后预测效能评价研究。方法:回顾性分析2019年1月~2020年9月期间入住浙江大学医学院附属第二医院急诊医学科的多发伤并完成HBP检测患者184例,根据SEPSIS 3.0诊断标准将患者分为脓毒症组(n=89)和非脓毒症组(n=95),追踪患者临床结局分为死亡组(n=43)和非死亡组(n=141)。连续测定患者HBP水平,比较两组HBP峰值差异,评估其诊断脓毒症的效力,以HBP峰值的中位数为界值进一步分析其与临床预后相关性,评估HBP单独及联合总胆红素(TBil)及白细胞(WBC)评估预后的效力。结果:(1)脓毒症组(n=89)与非脓毒症组(n=95)HBP的峰值(71.7±68.6 vs 52.5±56.1)无显著差异(P=0.051)。(2)184例患者中HBP峰值与WBC计数呈正相关(r=0.244,P<0.01),与TBil水平呈正相关(r=0.241,P<0.01)。(3)TBil水平、WBC计数及PCT水平独立诊断脓毒症曲线下面积(AUC)分别是:0.618、0.631和0.718,三者联合AUC为0.684,诊断敏感度为60.7%,特异度为71.6%(P<0.05)。(4)死亡预后相关分析显示:高HBP水平组患者死亡率要显著高于低水平组(30.4%vs 16.3%,P<0.05);WBC计数值也是死亡组显著高于非死亡组(17.5±6.9 vs 12.8±4.7,P<0.01),尤其合并脓毒症者,该值有显著差异(P<0.01)。HBP峰值、TBil水平、WBC计数、SOFA评分及APACHE-II评分对预测脓毒症死亡预后的AUC分别是:0.618、0.603、0.719、0.823及0.811,HBP联合TBil及WBC评估脓毒症预后的AUC为0.750,评估的敏感度为74.4%,特异度为74.5%(P<0.05)。(5)三者联合评估在预测脓毒症预后效力上与人工评分差异无统计学意义(P>0.05)。结论:HBP、TBil及WBC三者联合用于评估多发伤患者发生脓毒症风险的预测效力较高,对于合并脓毒症的外伤患者死亡风险预测具有较高的临床指导价值。

血乳酸、高迁移率族蛋白B 1对新生儿肺炎预后不良的预测价值

目的:分析血乳酸、高迁移率族蛋白B1(HMGB1)对新生儿肺炎(NP)预后不良的预测价值。方法:选取2021年3月至2023年3月该院收治的120例NP患儿为研究对象,纳入观察组,另选取同期于本院出生的120名健康新生儿为对照组,检测两组血乳酸、HMGB1水平。治疗后评估NP患儿的预后,比较不同预后NP患儿血乳酸、HMGB1水平。绘制受试者工作特征(ROC)曲线分析血乳酸、HMGB1单项及联合检测对NP患儿预后不良的预测价值。结果:观察组血乳酸、HMGB1水平均高于对照组,差异有统计学意义(P<0.05);预后不良NP患儿血乳酸、HMGB1水平均高于预后良好NP患儿,差异有统计学意义(P<0.05);ROC曲线分析结果显示,血乳酸、HMGB1单项及联合检测预测NP患儿预后不良的曲线下面积分别为0.791、0.717、0.825,且联合检测高于二者单项检测,差异有统计学意义(P<0.05)。结论:血乳酸、HMGB1联合检测对NP患儿预后不良的预测价值高于二者单项检测。

间隔12 h动态监测感染指标在新生儿早发型败血症诊断中的应用价值分析

目的探讨间隔12 h动态监测感染指标在新生儿早发型败血症(early-onset neonatal sepsis,EONS)诊断中的应用价值。方法回顾性分析120例新生儿的临床病历资料,将其中60例无严重并发症的EONS作为EONS组,60例无感染新生儿作为对照组。检测两组新生儿初次监测时和间隔12 h后感染指标[白细胞计数(WBC)、中性粒细胞百分比(NEU%)、血小板计数(PLT)、C反应蛋白(CRP)和降钙素原(PCT)]。比较两组新生儿同一时间点感染指标及两组不同时间点感染指标的变化。结果EONS组初次监测时WBC、NEU%、PLT、CRP和PCT分别为(12.5±3.2)×10^(9)/L、(60.2±7.5)%、(250±50)×10^(9)/L、(5.3±2.8)mg/L、(1.2±0.5)μg/L,与对照组初次监测时的(11.8±2.9)×10^(9)/L、(59.5±8.1)%、(248±48)×10^(9)/L、(4.7±2.6)mg/L、(1.1±0.4)μg/L比较,差异无统计学意义(P>0.05)。EONS组间隔12 h后WBC、NEU%、PLT、CRP和PCT分别为(18.5±4.3)×10^(9)/L、(75.8±8.6)%、(150±35)×10^(9)/L、(65.3±15.6)mg/L、(10.8±3.2)μg/L,与对照组间隔12 h后的(12.1±3.1)×10^(9)/L、(61.2±9.0)%、(240±55)×10^(9)/L、(5.8±3.2)mg/L、(1.0±0.3)μg/L比较差异具有统计学意义(P<0.05)。EONS组间隔12 h后WBC、NEU%、PLT、CRP和PCT与本组初次监测时比较,差异具有统计学意义(P<0.05)。对照组间隔12 h后WBC、NEU%、PLT、CRP和PCT与本组初次监测时比较,差异无统计学意义(P>0.05)。结论间隔12 h后动态监测感染指标有助于提高EONS的诊断准确性,有助于更早、更准确地诊断EONS。