Effects of Antimicrobial Peptides on Production,Slaughter Performance and Blood Routine of Tan Sheep

[Objectives]This study was conducted to analyze effects of antimicrobial peptides added to the diet of Tan sheep on their production, slaughter performance and blood composition. [Methods] Ninety two four-month-old Tan sheep were randomly divided into two treatment groups according to their body weight, 46 in each group. The control check group(CK) was feed with conventional diet, and the experimental group was fed with the addition of antimicrobial peptide on the basis of the conventional diet, with the added amount of 2.5 g/sheep per day. The experimental period was 60 d. [Results] The incidence rate was 75.06% lower in the experimental group than in the CK. The average daily weight gain per sheep was 11.27% higher in the experimental group than in the CK(P<0.05). The feed conversion ratio was 8.45% lower in the experimental group than in the CK(P<0.05). The average daily gross profit per sheep was 12.12% higher in the experimental group than in the CK. For slaughter performance, the data difference of each item was not significant. The PH at 45 min and 24 h after slaughter was within the normal range of fresh mutton. The cooked meat percentage and water loss rate showed no significant differences(P>0.05). The marbling ranged from 2.45 to 2.50, indicating that the fat content was moderate, and the difference between groups was not significant(P>0.05). The flesh color ranged from 3.00 to 3.15, between light red and bright red, belonging to the normal color of mutton, and the difference between groups was not significant(P>0.05). The shear force was between 2.50 and 2.65, without a significant difference between groups(P>0.05). The white blood cells, lymphocytes and platelets in the experimental group were lower than those in the CK(P<0.01). The erythrocytes and hemoglobin in the erythrocyte group were higher than those in the CK(P<0.05). The neutrophils and monocytes in the experimental group were lower than those in the CK(P<0.05). [Conclusions] This study provides a technical basis for the rational use of antimicrobial peptides and their application in ruminants.

Effects of Quebracho Tannins on Growth Performance and Blood Routine of Simmental Fattening Cattle

[Objectives]The effects of condensed quebracho tannins on the production performance and blood routine indices of Simmental fattening cattle were investigated experimentally.[Methods]Twenty four Simmental fattening cattle were selected and randomly divided into two treatment groups according to their body weight,with 12 animals in each group.The diet of the control group consisted of beef cattle concentrate supplement+silage+hay,and the diet of the experimental group consisted of beef cattle concentrate supplement+silage+hay+tannins,and the experimental period was 30 d.[Results]Compared with the control group,the addition of tannins[30 g/(cattle·d)]to the diet of beef cattle increased the daily weight gain of Simmental beef cattle by 24.68%(P<0.01),while the feed conversion ratio decreased by 2.57%(P>0.05).The gross profit per cattle increased by 973.63 yuan,and the economic benefit increased by 114.78%.Platelets were higher in the experimental group than in the control group(P<0.01);hemoglobin was lower in the experimental group than in the control group(P<0.01);leukocytes,neutrophils,monocytes and lymphocytes were lower in the experimental group than in the control group(P<0.01);and erythrocytes were lower in the experimental group than in the control group(P<0.05).[Conclusions]This study provides a theoretical basis for the rational utilization of quebracho tannins.

Analysis of Blood Lipids, Blood Glucose, Blood Uric Acid, and Blood Routine Test Results in Retired Employees of a Unit in the Civil Aviation System

Objective:To investigate and analyze the annual physical examination results of retired employees from a unit in the civil aviation system,focusing on blood lipids,blood glucose,blood uric acid,and blood routine results.The study aims to provide relevant references for formulating reasonable disease management measures for preventing and controlling hyperlipidemia,hyperuricemia,and other conditions in retired employees.Methods:The examination results of 231 participants were collected and analyzed.The participants were divided into four groups based on age:middle-aged group,young-old group,middle-old group,and old-old group.The blood test results were compared across these groups,and an assessment of atherosclerotic cardiovascular disease(ASCVD)risk levels was completed in conjunction with medical history.Blood test results were also compared by gender.Results:There were no significant statistical differences in blood test results when grouped by age.However,the prevalence of hyperuricemia was higher in males than in females,while the prevalence of hypercholesterolemia was higher in females than in males.The LDL-C target achievement rate was lower in the moderate-and-high-risk group as well as the very high-risk group as defined by ASCVD risk levels.Conclusion:Management of hyperuricemia and hyperlipidemia in retired employees(elderly patients)should be strengthened to reduce the risk of ASCVD events and alleviate the potential medical burden associated with disease progression.

Analysis of Serum Cys-C,TBA,and Routine Blood Parameters of Patients with Hepatitis B-Related Decompensated Cirrhosis

Objective:To study the levels of serum cystatin C(Cys-C),total bile acid(TBA),and other routine blood parameters on patients with decompensated hepatitis B cirrhosis.Methods:Study group 1 consisted of 30 patients with hepatitis B-related decompensated cirrhosis,and study group 2 consisted of 30 patients with hepatitis B;while the control group consisted of 30 healthy people who underwent physical examination.The blood parameters were used to evaluate the clinical treatment effect of patients.Results:The TBA,Cys-C,alanine transaminase(ALT),total bilirubin(TBIL),aspartate aminotransferase(AST),and international normalized ratio(INR)in study group 1 were significantly higher than those of study group 2 and the control group;while the platelet count(PLT),hemoglobin(Hb),albumin(ALB),and estimated glomerular filtration rate(eGFR)were significantly lower in the study group 1 compared to the control group and study group 2(P<0.05).The Cys-C,PLT,TBA,AST,TBIL,and INR of patients in study group 1 who were successfully treated were significantly lower than the patients who were not successfully treated(P<0.05).Conclusion:Serum Cys-C,TBA,and routine blood parameters are useful in predicting the condition and the prognosis of patients of hepatitis B-related decompensated cirrhosis.

Preoperative ultrasound combined with routine blood tests in predicting the malignant risk of pancreatic cystic neoplasms

Objective:Accurate preoperative identification of benign or malignant pancreatic cystic neoplasms(PCN)may help clinicians make better intervention choices and will be essential for individualized treatment.Methods:Preoperative ultrasound and laboratory examination findings,and demographic characteristics were collected from patients.Multiple logistic regression was used to identify independent risk factors associated with malignant PCN,which were then included in the nomogram and validated with an external cohort.The Net Reclassification Index(NRI)and Integrated Discrimination Improvement(IDI)were calculated to evaluate the improvement in the predictive power of the new model with respect to that of a combined imaging and tumor marker prediction model.Results:Malignant PCN were found in 83(40.7%)and 33(38.7%)of the model and validation cohorts,respectively.Multivariate analysis identified age,tumor location,imaging of tumor boundary,blood type,mean hemoglobin concentration,neutrophil-tolymphocyte ratio,carbohydrate antigen 19-9,and carcinoembryonic antigen as independent risk factors for malignant PCN.The calibration curve indicated that the predictions based on the nomogram were in excellent agreement with the actual observations.A nomogram score cutoff of 192.5 classified patients as having low vs.high risk of malignant PCN.The model achieved good C-statistics of 0.929(95%CI 0.890–0.968,P<0.05)and 0.951(95%CI 0.903–0.998,P<0.05)in predicting malignancy in the development and validation cohorts,respectively.NRI=0.268;IDI=0.271(P<0.001 for improvement).The DCA curve indicated that our model yielded greater clinical benefits than the comparator model.Conclusions:The nomogram showed excellent performance in predicting malignant PCN and may help surgeons select patients for detailed examination and surgery.The nomogram is freely available at https://wangjunjinnomogram.shinyapps.io/DynNomapp/.

Detection and Analysis of Routine Blood Indexes in Guike Pig

[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells（ red blood cells,white blood cells and platelets）,thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs（ 22 males and 22 females） once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count（ RBC） and hemoglobin count（ HGB） both reached the lowest levels（ 6. 85 × 10^12/L and 97. 1 g/L）at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age（ P 〈 0. 05,similarly hereinafter）; mean corpuscular hemoglobin（ MCH） and mean corpuscular hemoglobin concentration（ MCHC） demonstrated basically the same variation pattern,which both reached the lowest levels（ 15. 11 pg and 275. 70 g/L） at 70 days of age and reached the highest levels（ 16. 40 pg and 314. 30 g/L） at 91 days of age; hematocrit（ HCT）,mean corpuscular volume（ MCV） and red blood cell distribution width（ RDW） exhibited relatively stable changes with no significant differences among different day ages（ P 〉 0. 05,similarly hereinafter）. Among white blood cell indexes of Guike pig at different day ages,white blood cell count（ WBC） reached the lowest level（ 13. 71 × 10^9/L） at 63 days of age and reached the highest level（ 17. 52 × 10^9/L） at 84 days of age,with significant differences; neutrophil count（ NEUT）reached the lowest level（ 3. 61 × 10^9/L） at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count（ LYMPH） exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count（ PLT）,thrombocytocrit（ PCT） and platelet volume distribution width（ PDW） exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume（ MPV）,PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age.

Influence of Exercise at High Temperature on Blood Biochemical Indexes and HSP72 Expression in Adult Males

The influence of exercise at high temperature on adult males＇ routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes （PBLs） was studied in order to provide theoretical ground for health supervision of adults receiving exercise at high temperature. 180 adult males were selected and divided into exercise group and control group, in which the exercise group was subdivided into subgroup 1 and subgroup 2 receiving exercise at high temperature in the afternoon and in the morning, respectively. Peripheral venous blood was phle- botomized before and after the exercise to examine routine blood indexes and blood biochemical indexes. The expression levels of HSP72 in PBLs were detected by flow cytometry. The results showed that the routine blood indexes and biochemical indexes in each group were within the range of normal values of male adults. There was no significant difference between each exercise group and control group in indexes before exercise. After exercise, the expression levels of HSP72 in PBLs in exercise groups were higher than those before exercise, and HSP72 expression levels in subgroup 1 were obviously higher than those in subgroup 2 and control group. The contents of ALT, urea, Na＋, Cl-, Ca2＋ and K＋ in subgroups 1 and 2 were lower than those in control group, but CK level was higher than in control group （P〈0.05）. The contents of Na＋ and Clin subgroup 1 were relatively lower than those in subgroup 2 （P〈0.05）. It was concluded that while receiving exercise at high temperature, adult males＇ HSP72 levels in PBLs could be increased and the biochemical indexes changed. Attention should be paid to health supervision to avoid obvious body injuries at high temperature.

The Observation of Blood Biochemistry and HSP72 Expression Changing Rules in Peripheral Blood of Flying-saucer-athletes under High Temperature Training

Objective:To study the effect of training under high temperature on blood and heat shock protein 72(HSP72)expression of flying saucer athletes.Methods:The numbers of training group and control group are 30.Twenty-four flying saucer athletes in Zhejiang province were selected and randomly divided into training group and control group,15 in each group.Peripheral venous blood of each group was taken before and after the experiment.The HSP72 content of lymphocyte,blood routine and biochemical indexes were measured respectively.Results:There was significant difference in white blood cell in training group count before and after the experiment(P<0.05).The expression of HSP72 and the white blood cell in training group was higher than that control group(P<0.05).Conclusions:Organism could be affected and turned to stress state due to training under high temperature.It is necessary to provide the safeguard for the flying saucer athletes under high temperature in order to prevent the damage.

Trends in Coronavirus Disease 2019 Hospitalization and Prognosis:Gender Effect

We here aimed to investigate the impact of gender on the clinical characteristics and laboratory results of patients with coronavirus disease 2019(COVID-19)and provide clues to the pathological mechanisms underlying COVID-19.A retrospective study was performed.Clinical characteristics,severity of lung infection,laboratory results,and prognoses of patients of different gender were analyzed.A total of 242 patients were finally included.The median age was 58 years(IQR:40-68),including 54(22.3%)hospital staffs.Ninety-four(38.8%)were male and 148(61.1%)were female.The proportion of patients with diabetes was significantly higher in the male group than in the female group(P=0.034).Male patients had a significantly larger proportion of severe lung infection,higher leukocyte count,neutrophil count,neutrophil-to-lymphocyte ratio,C-reactive protein,and procalcitonin than femalc.Furthermore,male patients had worse liver,cardiac,and coagulation function than their female counterparts.Male patients with COVID-19 showed more severe inflammation reaction and coagulation dysfunction than female patients.In conclusion,gender is associated with host response to SARS-CoV-2 infection.

Effects of different hypoxia time on hematology and histomorphology of rats at 7 000 m altitude

Objective:To observe the effects of different hypoxia time on hematology and histomorphology of rats by simulating the environment of 7000m altitude,and to provide the basis for the establishment of acute hypoxia model.Methods:40 SD rats were randomly divided into 0h hypoxia group,6h,12h,24h,and 36h group.The hypoxia environment at 7000m was simulated by low-pressure oxygen chamber.The serum SOD,MDA,LDH biochemical indexes and the morphological changes of HE staining in heart,lung and brain tissues of rats were detected under different hypoxia time.Results:Compared with hypoxia group for 0h,the number of RBC in acute hypoxia group for 6h decreased and increased with the prolongation of hypoxia time,and the highest number of RBC.The WBC,LYMP,GRAN,HGB,HCT and RCT counts of the acute hypoxia group decreased for 6h,and the above indexes increased with the prolongation of hypoxia time.Compared with the 0h hypoxia group,SOD activity in serum decreased at different hypoxia time,and MDA content in the 24h and 36h hypoxia groups showed a fluctuating change and reached a peak at 12h hypoxia.The activity of LDH fluctuated,and reached the peak at 12hLDH after hypoxia,and then gradually decreased.He staining of the brain tissue of the heart and lung,which was close to normal after hypoxia for 36h,showed that with the prolongation of the hypoxia time,the damage degree of each organ increased continuously.Interstitial pneumonia or perivasculitis occurred in the lung,and perivascular edema and pulmonary interstitial widening were observed.Cardiac tissue showed obvious degeneration and necrosis of cardiomyocytes,lysis and necrosis of local myocardial interstitium and valve fibers.A large number of inflammatory cells could be seen infiltrating into the brain tissue in the CA2 CA3 DG region.The neuron cells were denatuated,the cell volume was reduced,basophilic was enhanced,and the nerve cells were triangular.The cells were pyknotic and basophilic,with a few transverse axis structures.Conclusion:acute low pressure hypoxia can cause red blood cells white blood cells within 6 h number of platelets Hemoglobin concentrations were lower,as after hypoxia prolonged period of count increased serum biochemical index SOD activity were lower,the content of MDA and activity of LDH outer-plane changes Can cause heart lung tissue pathological damage,and increased with prolonged.