Isolation of Multi-Drug Resistant (MDR) and Extensively Drug Resistant (XDR) <i>Salmonella typhi</i>from Blood Samples of Patients Attending Tertiary Medical Centre in Dhaka City, Bangladesh

Objective: Almost all year round occurrence of typhoid fever remains a major public health issue in Bangladesh. In recent years, there has been a significant rise in the prevalence of multi-drug resistance Salmonella typhi in Dhaka city. The major objective of this study was to observe the prevalence of multi-drug and Extended drug-resistant patterns of typhoidal Salmonella typhi among the patients attending a tertiary medical center by conventional culture and serologic test. Materials and Methods: Blood was collected by syringe and equally divided between aerobic & anaerobic BacT Alert bottle, 10 mL for paediatrics. Post isolation Salmonella strains were identified and were tested for antibiotic resistance. Results: A total of 600 blood samples of typhoid suspected patients were tested, where 32.5% came out positive, which were identified as Salmonella typhi. These isolates were susceptible to carbapenem drugs but highly resistant to Fluoroquinolones and Aminoglycosides antibiotics. Discussion: It was seen that 73.23% of isolates among male patients were extensively drug-resistant (XDR) meaning that isolates were sensitive against only one or two antibiotics while in the female it was 58.46%. Isolates from Female patients showed higher multi-drug resistant (MDR) (26.15%) isolates resistance to multiple antibiotics in comparison to isolates from the male (16.54%). 87.19% of the strains showed high drug resistance (XDR and MDR). Conclusion: The emergence of XDR and MDR strains of S. typhi in recent years is becoming a significant threat. Although no PAN-Drug resistance (resistant against all tested drugs) was observed in the current study, a high level of XDR and MDR isolates are indicating that the scenario might take place sooner than later.

献血者HBVs基因变异的生物信息学分析

目的分析HBV DNA+/HBsAg-献血者的传染性指标、S区基因序列突变情况及生物信息学特征变化。方法通过PCR方法筛查出1份HBV DNA+/HBsAg-的标本,应用酶联免疫和化学发光方法检测该标本乙肝病毒5项指标,对该标本HBVs区基因片段测序并分析变异情况,应用分析软件对所测序列进行生物信息学分析。结果该标本HBV DNA+、HBsAg-、HBsAb+、HBeAg+、HBeAb-、HBcAb+;HBVs区基因序列内发生氨基酸单位点突变(P151L),空间结构发生改变。结论HBVs区基因序列空间结构改变,可能是导致检测结果出现血清学HBsAg-/HBsAb+/HBV DNA+的原因。

S-100β CysC和NF-κB对急性缺血性脑卒中患者静脉溶栓后出血转化的预测价值

目的 探讨急性缺血性脑卒中(AIS)外周血中S-100β、CysC和NF-κB水平对静脉溶栓后出血转化的影响及预测价值。方法 收集2019-03—2022-03接受溶栓治疗的AIS患者140例,根据溶栓后24 h是否发生出血转化(HT)将患者分为非HT组(n=112)和HT组(n=28)。比较2组一般临床资料,采用多因Logistic回归分析影响HT发生的危险因素,受试者工作特征(ROC)曲线评估S-100β、CysC和NF-κB预测HT发生的价值。结果 HT组与非HT组相比,患者年龄、发病至溶栓时间、房颤、TOAST分型、C反应蛋白、凝血酶原时间、S-100β、CysC、NF-κB、白质高信号和脑微出血等均有统计学差异(P<0.05)。Logistic多因素回归分析显示,房颤、S-100β、CysC和NF-κB为影响HT发生的危险因素。S-100β、CysC和NF-κB预测AIS患者静脉溶栓后出血转化的曲线下面积分别为0.915(0.902~0.923)、0.874(0.856~0.882)和0.789(0.771~0.796),均具有一定的预测价值。结论 S-100β、CysC和NF-κB为AIS患者静脉溶栓后HT发生的危险因素,对HT发生具有一定的预测价值。

Occurrence of Salmonella Enteric Serovar Typhi Antibodies among Blood Donors in Ghana

Background: Donated blood contaminated with S. Typhi can cause post-transfusion sepsis. This study aimed to determine the correlation between some risk factors of typhoid fever and seroprevalence of antibodies against S. Typhi among blood donors. Methodology: Following informed consent, socio-demographic and information on risk factors of typhoid infection was obtained using pre-structured questionnaires from 400 apparently healthy blood donors at the Tema General Hospital. Blood was also collected for serology and cultured for identification of pathogens by standard bacteriological method. Results: Blood culture did not reveal any S. Typhi isolate out of the tested 400 (348 males and 52 females) samples from apparently healthy blood donors. However, IgM and IgG antibody seroprevalence of 9.3% and 3.5% were detected. Age group of 17 - 24 years was the highest risk group, persons with a history of typhoid infection, and sources of drinking water were major risk factors for typhoid infection. It was also observed that prevalence of IgM was highest among new donors (62.2%), but lower in donors with a history of 1 to 3 blood donations (32.4%) and least among regular donors (>3 donations (5.4%)). In addition, typhoid prevention awareness and typhoid knowledge (knowledge about typhoid transmission) among the donors were poor (4.3% and 5.9% respectively). Conclusions: This study has shown an overall seroprevalence of 9% and 3.5% for IgM and IgG antibodies respectively among blood donors in the Tema area in Ghana. We advocate for the mandatory screening of donor units intended for transfusion for S. Typhi. Furthermore, there is an urgent need for the health education of all persons in Ghana on preventive measures and the spread of S. Typhi.

Blood Stasis Syndrome in Japan and Its Molecular Biological Analysis

Blood stasis syndrome is one of the pathological concepts of Oriental traditional medicine. In Oriental traditional medicine, blood is thought of as not only blood but also as a living component of the body. In fact, blood stasis syndrome is related to not just circulation disorders but dermatological and gynecological and other diseases. In Japan, the concept of blood stasis syndrome is based on the past literature, for instance, Synopsis of Golden Chamber （Jin Kui Yao Lue）, etc. There are many signs of this syndrome, such as a dry mouth, fullness of the abdomen and rough skin. However, the levels of importance of these signs had been unclear. Therefore, in order to determine the levels of seriousness, a scoring system of blood stasis syndrome was made based on multivariate analysis by Dr. Terasawa （Terasawa＇s Blood Stasis Score）. Using the scoring system, we have studied blood stasis syndrome mainly related to blood circulation using modem techniques of analysis. From the results, we found that patients with blood stasis syndrome showed hemorheological abnormalities, and an improvement in these abnormalities was shown after administration of removing-blood stasis formulae. Furthermore, we have studied blood stasis syndrome from the point of view of molecular biology. We searched for the specific protein expression in blood stasis syndrome by proteomic analysis, and found no specific protein expression. However, there may be a possibility of developing a diagnostic algorithm for blood stasis by construction of a decision tree. During the past few years, as one of the molecular biological factors affecting blood stasis syndrome, we have been studying hypoxia inducible factor, which is located in the upstream of many genes. Above all, blood stasis syndrome is more than just circulatory deficit but encompasses the pathological concept of constant multilateral change in the living body.

红细胞比容、血流S/D比值及同型半胱氨酸在妊娠期糖尿病患者中的表达及对妊娠结局的影响

目的探究红细胞比容、血流S/D比值及同型半胱氨酸在妊娠期糖尿病患者中的表达及对妊娠结局的影响。方法选取我院2021年1月~2023年1月纳入的86例妊娠期糖尿病患者为研究组,另外选取同时期86例妊娠期健康孕妇作为对照组,分别对两组孕妇以及研究组不同疾病严重程度孕妇之间的红细胞比容、血流S/D比值、同型半胱氨酸表达水平进行比较,观察记录两组孕妇不良妊娠结局的发生情况。结果研究组孕妇的红细胞比容明显低于对照组,血流S/D比值及同型半胱氨酸表达水平均明显高于对照组,差异有统计学意义(P<0.05);轻度、中度、重度妊娠期糖尿病患者的红细胞比容、血流S/D比值及同型半胱氨酸水平存在明显差异,且随着疾病严重程度的增高,红细胞比容越低,血流S/D比值及同型半胱氨酸水平越高,差异有统计学意义(P<0.05);研究组孕妇的不良妊娠结局总发生率明显高于对照组,差异有统计学意义(P<0.05)。结论红细胞比容、血流S/D比值及同型半胱氨酸在妊娠期糖尿病患者中的表达水平异于健康孕妇,同时还与孕妇的妊娠结局存在一定的关联,血流S/D比值及同型半胱氨酸较高水平的表达与红细胞比容较低的情况增加了妊娠期糖尿病孕妇不良妊娠结局的发生率。

长期冷冻对ELISA法检测血浆标本中HBsAg结果的影响

目的评价献血者HBsAg阳性血液标本在-20°C冻存8年后ELISA法HBsAg检测的结果,评估血站目前留样保存方式的有效性。方法收集本站2014年5月—2015年3月100份经HBsAg ELISA检测阳性的献血者血浆标本,冻存在-20°C冰箱,于2023年解冻标本并通过同种方法再次检测。结果100份血浆标本的HBsAg再检定性结果均为阳性,再检符合率100%,冻存后S/CO值降低明显(27.52 vs 19.03,P<0.05)。结论长期冻存会使HBsAg ELISA检测S/CO值下降,但不影响阳性定性结果。

Correlations of PCBs, DIOXIN, and PBDE with TSH in Children's Blood in Areas of Computer E-waste Recycling

Objective To study correlations of polychlorinated biphenyls （PCBs）, DIOXIN, and polybrominated diphenyl ethers （PBDE） with thyroid stimulating hormone（TSH） in children, and assess the impact on children＇s health. Methods Three hundred and sixty nine children aged from 6 to 8, including 195 from Luqiao, the computer E‐waste recycling area, and 174 from Longyou, the control area, were selected for this investigation to elucidate the correlation of PCBs, DIOXIN, and PBDE with TSH in children’s blood samples. The children had a physical examination and their blood levels of PCBs, DIOXIN, PBDE, and TSH were detected after sample collection. Results In the E‐waste recycling area, the contents of PCBs, PBDE, DIOXIN, and TSH in the blood samples of children were 484.00±84.86 ng·g ‐1 lipid weight, 664.28±262.38 ng·g ‐1 lipid weight, 26.00±19.58 ng·g ‐1 lipid weight and 1.88±0.42 μIU/mL （serum） respectively, while in the control area, the PCBs, PBDE, DIOXIN, and TSH contents were 255.38±95 ng·g ‐1 lipid weight, 375.81±262.43 ng·g ‐1 lipid weight, 39.64±31.86 ng·g ‐1 lipid weight, and 3.31±1.04 μIU/mL respectively. Conclusion The health status of children in the control area are better than that in the contaminated area. Among children who are exposed to persistent organic pollutants, the pollutant content increases significantly in their serum, and the distribution of TSH levels in their bodies are also affected.

Protective effects of Dragon's Blood on blood coagulation and NO/iNOS level in myocardium and serum of rats in simulated microgravity

To investigate effects of Dragon＇s Blood（DB）,a traditional Chinese medicine,on blood coagulation and NO/iNOS level in myocardium and serum of rats in simulated microgravity for the first time,Sprague Dawley（SD）rats were randomly divided into six groups：（a）5-day control group,（b）5-day model group,（c）5-day drug group,（d）21-day control group,（e）21-day model group,and（f）21-day drug group.Blood coagulation and NO/iNOS level in myocardium and serum were examined after 5 and 21 days of simulated microgravity respectively.The results showed that blood of tail-suspended rats was in a hypercoagulable state that could not be converted with time extending.Conversely,DB changed these parameters towards normal level and the curative effects became better when tail-suspension lasted till the 21 st day.NO concentration of both myocardium and serum for two periods all increased markedly and DB could effectively reduce these increases except that of 21-day myocardium NO.Activity of iNOS increased markedly as early as 5 days and became more serious on the 21 st day,while DB showed preventive effect on the 21 st day.Western Blot analysis illustrated that the expression of iNOS in the 5-day model group increased significantly over the 5-day control group and the expression in the 5-day drug group dramatically returned to the normal level.The similar trend was observed on the 21-day groups without notable variances.The findings of this study can serve for the further use of Dragon＇s Blood in space diseases.

脐动脉血流S/D值联合孕妇血清CX3CL1 HIF-1α对胎儿窘迫的诊断价值

目的探讨脐动脉血流收缩期末最大血流速度/舒张期末最大血流速度(S/D)、血清趋化因子C-X3-C配体1(CX3CL1)、血清缺氧诱导因子-1α(HIF-1α)对胎儿窘迫(FD)的诊断价值。方法选取2020年5月至2022年5月在青岛市妇女儿童医院妇产科分娩且出现FD的128例孕妇为研究对象(FD组),根据新生儿Apgar评分将FD孕妇分为轻度窘迫组74例和重度窘迫组54例,另取同期在本院定期产检并分娩的健康且未出现FD的孕妇78例为正常组;超声检测FD孕妇脐动脉血流S/D值;ELISA法检测FD孕妇血清CX3CL1、HIF-1α水平;Pearson法分析FD孕妇血清CX3CL1、HIF-1α水平与脐动脉血流S/D值的相关性;受试者工作特征(ROC)曲线分析血清CX3CL1、HIF-1α水平及脐动脉血流S/D值对胎儿重度窘迫的诊断价值。结果FD组孕妇脐动脉血流阻力指数(RI)、搏动指数(PI)、S/D值、血清CX3CL1及HIF-1α水平均显著高于正常组(P<0.05),重度窘迫组孕妇RI值、PI值、S/D值、血清CX3CL1及HIF-1α水平均高于轻度窘迫组(P<0.05)。FD组血清CX3CL1、HIF-1α与脐动脉血流S/D值均呈正相关(r=0.531、0.489,P<0.05)。脐动脉血流S/D值、血清CX3CL1、HIF-1α以及3项联合诊断胎儿重度窘迫的AUC分别为0.870、0.802、0.731、0.941,联合诊断的灵敏度为87.04%,特异度为90.54%,3项联合优于脐动脉血流S/D值、血清CX3CL1、HIF-1α各自单独诊断(Z3项联合-S/D=2.775、Z3项联合-CX3CL1=3.998、Z3项联合-HIF-1α=4.925,P均<0.05)。结论FD孕妇脐动脉血流S/D值、血清CX3CL1、HIF-1α水平均上升,且血清CX3CL1、HIF-1α水平与脐动脉血流S/D值均呈正相关,3项联合对诊断胎儿重度窘迫有更高的价值。

LARGELY IMPROVED BLOOD COMPATIBILITY OF POLYURETHANE BY BLENDING WITH FLUORINATED PHOSPHATIDYLCHOLINE POLYURETHANE

The improvement of biocompatibility of polyurethanes was investigated.The results demonstrate that the blood compatibility of polyurethanes can be further improved by just simply mixing with the fluorinated phosphatidylcholine poly(carbonate urethane)s(FPCPCUs).The solution blending was done by mixing poly(ether urethane)(PEU)with FPCPCU in different compositions.An increased blood compatibility of the blend films was observed with the increase of FPCPCU content,and when FPCPCU content reached to 40 wt%(40F...

Bioinformatics Analysis Raises Candidate Genes in Blood for Early Screening of Parkinson's Disease

Parkinson＇s disease （PD） is a typical degenerative disease, which is characterized by the most obvious symptoms of movement dysfunction, including shaking, rigidity, slowness of movement and difficulty in walking and gait. This disease can not be clearly identified through laboratory tests at present, thus application of high-throughput technique in studying the expression profiles of PD helps to find the genetic markers for its early diagnosis. Studies on expression profiles of neurodegenerative diseases have revealed the novel genes and pathways involved in the progress of illness. In this study, the expression profiles of PD in blood were compared, showing that 181 differentially expressed genes （DEG） exhibit a similar expression trend both in patients and in normal controls.

Blood Compatibility of Amphiphilic Poly(N-α-acrylamide-L-lysine-<i>b</i>-dimethylsiloxane) Block Copolymers

Amphiphilic block copolymers poly(LysAA-b-DMS) consisting of a hydrophilic poly(N-α-acrylamide-L-lysine) [poly(LysAA)] segment with different molecular weights and a hydrophobic polydimethylsiloxane (PDMS) segment were prepared as follows. The precursor copolymer poly(Boc-LysAA-OtBu-b-PDMS) was obtained from radical polymerization of N-α-acrylamide-N-ε-tert-butoxycarbonyl-L-lysine-tert-butylester (Boc-LysAA-OtBu) initiated with 4,4’-azobis(polydimethylsiloxane 4-cyanopentanoate) (azo-PDMS) with the molecular weight of PDMS Mw = 4.3 × 103 in the presence of 2-mercaptoethanol (2-ME) as a chain-transfer agent. Removal of the protecting groups of the precursor copolymer was carried out in 80% trifluoroacetic acid aqueous solution to give poly(LysAA-b-DMS)-1-3. The weight average molecular weight of poly(LysAA-b-DMS)-1-3 was Mw = 1.02 × 104 – 2.52 × 104. From the 1H-NMR and fluorescence spectra measurements, poly(LysAA-b-DMS)-1-3 was determined to self-organize and form core-shell micelles in water. The critical micelle concentration (CMC) increased to 1000 - 4000 mg·L–1 with increasing molar ratio of the poly(LysAA) segment from 0.42 to 0.65. From morphological analysis with a scanning probe microscope (SPM), poly(LysAA-b-DMS) has microphase-separated structures made up of hydrophilic and hydrophobic regions with the domain size ranging from several tens to several hundreds of nanometers. Inhibition of thrombin activity of poly(LysAA-b-DMS) was evaluated from the Michaelis constant (KM) and catalytic activity (kcat) for the enzymatic reaction of thrombin and synthetic substrate S-2238 in the presence of poly(LysAA-b-DMS). The KM and kcat were 0.10 - 0.11 mM and 4.04 × 105 – 4.26 × 105 min–1, respectively. Fibrinolytic activity was also verified from the transformation of plasminogen to plasmin by tissue plasminogen activator (t-PA) using synthetic substrate S-2251 in the presence of poly(LysAA-b-DMS). The KM and kcat were 0.07 mM and 5.73 × 106 –5.95 × 106 min–1, respectively.

急性ST段抬高型心肌梗死患者救治与降压达标的相关性研究

目的分析急性ST段抬高型心肌梗死(ST-elevation myocardial infarction,STEMI)救治时间节点与降压达标的相关性。方法选择CCU于2017年2月至2018年8月收治的行急诊经皮冠状动脉介入术(PCI)的STEMI患者374例,采用统一调查问卷收集资料,根据合并高血压情况分为高血压组168例和非高血压组206例。依据高血压组血压控制达标情况,分为达标组(75例)和未达标组(93例),分析2组间的差异,Logistic回归分析降压达标与首次医疗接解(S-to-FMC)、D-to-B、总缺血时间(TIT)的相关性。结果STEMI患者中高血压患者的检出率44.9%;2、3级高血压患者占92.7%;高血压组与非高血压组比较,2组S-to-FMC、D-to-B、TIT差异无统计学意义(P>0.05);降压达标组S-to-FMC、TIT时间显著低于非达标组(P<0.05)。结论降压达标患者的获益不仅是在降压本身及重要脏器的保护作用,与心血管事件的及时救治有一定的相关性,降压达标的STEMI患者院前延迟时间、心肌总缺血时间缩短,有助于改善患者的预后。

外周血单个核细胞GTSE1水平与上皮性卵巢癌预后的相关性

目的:探究外周血单个核细胞G_(2)/S期应答相关蛋白1(G_(2)and S phase-expressed-1,GTSE1)水平与上皮性卵巢癌(epithelial ovarian cancer,EOC)预后的关系。方法:GEPIA和Kaplan-Meier Plotter分析GTSE1在卵巢癌组织中的表达及其与预后的关系。选择2017年04月至2020年10月本院126例EOC患者(EOC组)和40例同期无EOC的健康体检者(对照组)作为研究对象。Ficoll密度梯度离心法分离外周血中单个核细胞,蛋白质免疫印迹法检测GTSE1水平。Spearman相关分析EOC组织和外周血单个核细胞中GTSE1水平的关系。随访了解EOC预后,用COX回归分析EOC预后的风险因素。结果:GEPIA和Kaplan-Meier Plotter分析结果显示,卵巢癌组织中GTSE1水平高于正常卵巢组织(P<0.05),GTSE1低水平卵巢癌患者的中位无进展生存时间高于GTSE1高水平卵巢癌患者(P<0.05)。随访期间复发98例(77.8%),其中铂类敏感型65例,铂类耐药型33例;死亡41例(32.5%)。EOC组的外周血单个核细胞中GTSE1 mRNA和GTSE1蛋白水平均高于对照组(P<0.001)。EOC患者癌组织和外周血单个核细胞中GTSE1水平呈正相关关系(r_(s)=0.225,P=0.011)。国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)Ⅰ-Ⅱ期患者的GTSE1水平低于Ⅲ-Ⅳ期的患者(P<0.001);未复发患者的GTSE1水平低于铂类敏感型和铂类耐药型的患者(P<0.05);生存患者的GTSE1水平低于死亡患者(P<0.001)。GTSE1高水平患者的无复发生存时间和总生存时间均低于GTSE1低水平患者(P<0.001)。COX回归分析结果显示,FIGO分期高(HR=2.110,95%CI:1.465~3.038,P<0.001;HR=2.261,95%CI:1.212~4.216,P=0.011)、初次手术残存灶>1 cm(HR=1.724,95%CI:1.130~2.631,P=0.012;HR=2.465,95%CI:1.246~4.874,P=0.010)和GTSE1>1.29(HR=2.071,95%CI:1.304~3.291,P=0.002;HR=5.634,95%CI:2.410~13.169,P<0.001)是EOC预后(复发和生存)的独立危险因素,应用贝伐单抗(HR=0.293,95%CI:0.116~0.738,P=0.010;HR=0.141,95%CI:0.056~0.357,P<0.001)是EOC预后的独立保护因素。结论:EOC患者外周血单个核细胞中GTSE1水平高提示预后不良风险高。

调节肠道菌群代谢产物TMAO/H_(2)S促进卒中后抑郁大鼠血脑屏障和神经功能恢复

目的 研究肠道菌群代谢产物氧化三甲胺(TMAO)/硫化氢(H_(2)S)对卒中后抑郁(PSD)大鼠血脑屏障、神经功能的作用。方法 SD大鼠分成对照组、中动脉栓塞(MCAO)组、PSD组、粪便移植组、抗生素(利福昔明)组、益生菌组和氟西汀组,对大鼠进行神经功能评分,检测大鼠的糖水偏好度和强迫游泳静止不动时间;采用ELISA法检测大鼠血浆中TMAO、H_(2)S水平;采用Western-blot实验检测大鼠脑组织中闭锁蛋白(Occludin)、封闭蛋白5(claudin 5)、免疫球蛋白G(IgG)等蛋白的表达。结果 与对照组比较,MCAO组、PSD组大鼠的神经功能评分升高,强迫游泳静止不动时间增加,TMAO水平升高,H_(2)S水平降低,TMAO/H_(2)S比值升高(P<0.05);PSD组大鼠糖水偏好度降低,差别有统计学意义(P<0.05)。与MCAO组比较,PSD组大鼠神经功能评分升高,强迫游泳静止不动时间增加,糖水偏好度降低,TMAO水升高,H_(2)S水平降低,TMAO/H_(2)S比值升高,差别有统计学意义(P<0.05)。PSD大鼠TMAO/H_(2)S比值与神经功能评分、强迫游泳静止不动时间和IgG蛋白相对表达量呈正相关(r=0.323 5,P=0.026 9;r=0.629 0,P=0.000 4;r=0.472 4,P=0.004 6),与糖水偏好度、Occludin和claudin 5蛋白的相对表达量呈负相关(r=-0.453 4,P=0.005 9;r=-0.356 4,P=0.018 8;r=-0.290 9,P=0.038 0)。与对照组比较,PSD组大鼠IgG蛋白相对表达量升高,Occludin、claudin 5蛋白相对表达量降低,差别有统计学意义(P<0.05);与PSD组比较,粪便移植组、抗生素组、益生菌组和氟西汀组大鼠神经功能评分和强迫游泳静止不动时间均降低,糖水偏好度升高,TMAO/H_(2)S比值降低,IgG蛋白相对表达量降低,Occludin、claudin 5蛋白相对表达量升高,差别有统计学意义(P<0.05)。结论 PSD大鼠TMAO/H_(2)S比值与神经功能评分、强迫游泳静止不动时间、糖水偏好度相关,调节肠道菌群可改善PSD大鼠神经功能和抑郁症状,促进大鼠血脑屏障恢复。

中性粒细胞胞外诱捕网与Virchow′s triad的联系及其在静脉血栓栓塞症中的研究进展

静脉血栓栓塞症(VTE)包含深静脉血栓形成(DVT)与肺血栓栓塞症,是常见的一种心血管疾病。其中,DVT的Virchow三要素包括血管内皮损伤、血液高凝状态和静脉血流滞缓。此外,激活的中性粒细胞释放中性粒细胞外诱捕网(NETs)也参与VTE的发病过程。NETs由解聚的染色质和各种蛋白质组成,它可以参与血管内皮损伤、促进血液达到高凝状态并导致静脉血流滞缓,从而影响VTE的进展。因此,更好地理解NETs在VTE病理生理过程中的作用对于其早期诊断、预防和治疗具有重要意义。

超声参数联合外周血指标诊断儿童淋巴瘤的价值分析

目的探讨超声参数联合外周血指标对儿童淋巴瘤的诊断价值。方法对2018年6月至2020年10月该院收治的疑为淋巴瘤的浅表淋巴结肿大123例患儿进行超声检查,其中85例患儿经病理诊断为淋巴瘤(淋巴瘤组),38例患儿诊断为良性病变(良性组),同期选取30例健康儿童作为健康组。评价长短径比(L/S)、淋巴门、血流类型等超声参数对儿童非霍奇金淋巴瘤的诊断价值。通过逆转录-聚合酶链反应分析3种免疫相关基因——CC趋化因子受体5(CCR5)、程序性细胞死亡蛋白1(PD-1)、叉头盒P3(FOXP3)在淋巴瘤儿童外周血中的表达,分析超声参数联合3种外周血指标对儿童淋巴瘤的联合诊断价值。结果L/S、淋巴门、血流类型联合诊断淋巴瘤的受试者工作特征曲线下面积、灵敏度和特异度分别为0.846、89.41%、68.42%。淋巴瘤组患儿外周血CCR5、PD-1、FOXP3 mRNA相对表达量均明显高于健康组和良性组,差异均有统计学意义(P<0.05)。超声参数联合外周血CCR5、PD-1、FOXP3诊断淋巴瘤的受试者工作特征曲线下面积、灵敏度和特异度分别为0.976、97.65%、92.11%。结论超声参数(L/S、淋巴门和血流类型)联合外周血CCR5、PD-1、FOXP3对淋巴瘤具有较高的诊断价值。

对血液制品S/D病毒灭活方法的验证

以水疱性口炎病毒（VSV）为指示病毒，验证S／D法处理对凝血因子Ⅷ、纤维蛋白原和凝血酶原复合物三种血液制品的病毒灭活效果。结果该法对三种制品中的VSV灭活能力分别为5．13、5．38和3．25LogTCID50／0．1ml以上（均为可检测限）。表明S／D方法对VSV等脂包膜病毒可起到有效的灭活作用。

胰岛素注射相关皮下脂肪增生对糖尿病患者短期血糖波动的影响

目的探讨胰岛素注射相关皮下脂肪增生对糖尿病患者短期血糖波动的影响。方法选择2022年5月至2023年3月于我院进行胰岛素注射治疗且行动态血糖监测的30例胰岛素注射相关皮下脂肪增生1型糖尿病患者作为1型增生组,另选择同期同类治疗的30例无胰岛素注射相关皮下脂肪增生的1型糖尿病患者作为1型非增生组。选择同期我院胰岛素注射治疗且行动态血糖监测的30例胰岛素注射相关皮下脂肪增生2型糖尿病患者作为2型增生组,另选择同期同类治疗的30例无胰岛素注射相关皮下脂肪增生的2型糖尿病患者作为2型非增生组。统计患者血糖波动相关指标情况。结果1型增生组LAGE、MADE、MODD水平显著高于1型非增生组(P<0.05)。两组SD、CV、LI、CONGAn1h、CCONGAn4h比较,差异无统计学意义(P>0.05)。1型增生组短期血糖波动发生率水平(53.33%,16/30)显著高于1型非增生组(10.00%,3/30)(P<0.05)。2型增生组血糖波动相关指标与2型非增生组比较(P<0.05)。2型增生组短期血糖波动发生率水平(73.33%,22/30)显著高于2型非增生组(13.33%,4/30)(P<0.001)。结论胰岛素注射相关皮下脂肪增生对1型和2型糖尿病患者均对血糖指标有影响,可增加短期血糖波动风险,对于血糖控制较为不利,需积极防控该类脂肪增生发生发展。