A new fluorescent reagent, 7 (8 hydroxy 3, 6 disulfonaphthylazo) 8 hydroxyquinoline 5 sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluor...A new fluorescent reagent, 7 (8 hydroxy 3, 6 disulfonaphthylazo) 8 hydroxyquinoline 5 sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex with λ ex / λ em =356/495 nm immediately at room temperature in ammonia ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0 160 ng·mL -1 with the detection limit of 0.04 ng·mL -1 . The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively.展开更多
Aim: To explore the research progress of echocardiography combined with blood-related detection indicators in the diagnosis and treatment of Kawasaki disease in children. By collecting relevant literature reports, ech...Aim: To explore the research progress of echocardiography combined with blood-related detection indicators in the diagnosis and treatment of Kawasaki disease in children. By collecting relevant literature reports, echocardiography and several blood-related detection indicators such as SAA, IL-6, PCT and CRP were screened out. It has good clinical application value in the clinical diagnosis and treatment of Kawasaki disease in children, but there is no report on the application of echocardiography combined with blood SAA, IL-6, PCT and CRP detection in the diagnosis and treatment of Kawasaki disease in children. The author intends to explore the role of echocardiography combined with blood SAA, IL-6, PCT and CRP detection in the diagnosis and treatment of Kawasaki disease in children, hoping to achieve better results.展开更多
To investigate the role of IL-17 in the overproduction of autoantibodies and IL-6 overexpression by peripheral blood mononuclear cells (PBMC) of lupus nephritis (LN) patients Methods Fifteen consecutively hospitaliz...To investigate the role of IL-17 in the overproduction of autoantibodies and IL-6 overexpression by peripheral blood mononuclear cells (PBMC) of lupus nephritis (LN) patients Methods Fifteen consecutively hospitalized LN patients were selected as subjects and 15 healthy adults as normal controls PBMC were obtained by Ficoll density gradient centrifugation IgG, anti-dsDNA antibody and IL-6 protein levels were assessed using enzyme-linked immunosorbent assays (ELISA) on the supernatant of cul tured PBMC of LN patients or normal controls IL-6 mRNA levels in PBMC were measured using reverse transcription-polymerase chain reaction (RT-PCR) Results In medium culture, IgG, anti-dsDNA and IL-6 protein levels of the supernatant of PBMC from LN patients were significantly higher than those from normal controls (1492 1±73 2 ng/ml vs 636 7±51 9 ng/ml for IgG, 306 6±53 7 IU/ml vs 95 8±11 6 IU/ml for anti-dsDNA and 50 92±15 92 ng/ml vs 1 77±0 73 ng/ml for IL-6, all P<0 001) In LN patients, IgG, anti-dsDNA and IL-6 protein levels were higher in the supernatants of PBMC in the IL-17-stimulated culture than the medium culture, but in normal controls, only the IL-6 protein levels were significantly higher The increase in IgG, anti-dsDNA and IL-6 protein levels induced by IL-17 was dose-dependent and could be completely blocked by IL-17 monoclonal antibody mIgG 28 and partially blocked by dexamethasone Similarly, IL-6 mRNA overexpression of PBMC in LN patients or normal controls induced by IL-17 was both dose- and time-dependent During medium culture, IL-6 mRNA levels in LN patients were significantly higher than those in normal controls (1 80±0 11 vs 0 36±0 07) During stimulation with IL-17, IL-6 mRNA levels in LN patients were higher than those in normal controls (3 21±0 24 vs 1 30±0 14, P<0 05) and also significantly higher when comparing the stimulated culture with the medium culture either in LN patients or normal control Conclusions IL-17 may play an important role in the pathogenesis of LN through the induction of IgG, anti-dsDNA overproduction and IL-6 overexpression of PBMC in LN patients展开更多
Interleukin 6(IL-6) is known as hybridoma cell growth factor,B-cell differentiating factor and so on One of its important biological functions in to induce
基金Supported by the Zi-Qiang Foundation of Wuhan U niversit
文摘A new fluorescent reagent, 7 (8 hydroxy 3, 6 disulfonaphthylazo) 8 hydroxyquinoline 5 sulfonic acid (HDNHQ) for the determination of magnesium has been developed. It reacted with magnesium to form a 1∶1 fluorescent complex with λ ex / λ em =356/495 nm immediately at room temperature in ammonia ammonium chloride buffer (pH 10.7). A linear relationship was obtained in the magnesium concentration range of 0 160 ng·mL -1 with the detection limit of 0.04 ng·mL -1 . The proposed method was simple, rapid and sensitive. It has been successfully applied to the determination of trace magnesium in blood serum with recoveries of 103.75% and 98.16%, respectively.
文摘Aim: To explore the research progress of echocardiography combined with blood-related detection indicators in the diagnosis and treatment of Kawasaki disease in children. By collecting relevant literature reports, echocardiography and several blood-related detection indicators such as SAA, IL-6, PCT and CRP were screened out. It has good clinical application value in the clinical diagnosis and treatment of Kawasaki disease in children, but there is no report on the application of echocardiography combined with blood SAA, IL-6, PCT and CRP detection in the diagnosis and treatment of Kawasaki disease in children. The author intends to explore the role of echocardiography combined with blood SAA, IL-6, PCT and CRP detection in the diagnosis and treatment of Kawasaki disease in children, hoping to achieve better results.
基金Thisstudywassuportedfromgrantsfor211keyprojectsofSunYatSenUniversityofMedicalSciences (No .981 51 7)
文摘To investigate the role of IL-17 in the overproduction of autoantibodies and IL-6 overexpression by peripheral blood mononuclear cells (PBMC) of lupus nephritis (LN) patients Methods Fifteen consecutively hospitalized LN patients were selected as subjects and 15 healthy adults as normal controls PBMC were obtained by Ficoll density gradient centrifugation IgG, anti-dsDNA antibody and IL-6 protein levels were assessed using enzyme-linked immunosorbent assays (ELISA) on the supernatant of cul tured PBMC of LN patients or normal controls IL-6 mRNA levels in PBMC were measured using reverse transcription-polymerase chain reaction (RT-PCR) Results In medium culture, IgG, anti-dsDNA and IL-6 protein levels of the supernatant of PBMC from LN patients were significantly higher than those from normal controls (1492 1±73 2 ng/ml vs 636 7±51 9 ng/ml for IgG, 306 6±53 7 IU/ml vs 95 8±11 6 IU/ml for anti-dsDNA and 50 92±15 92 ng/ml vs 1 77±0 73 ng/ml for IL-6, all P<0 001) In LN patients, IgG, anti-dsDNA and IL-6 protein levels were higher in the supernatants of PBMC in the IL-17-stimulated culture than the medium culture, but in normal controls, only the IL-6 protein levels were significantly higher The increase in IgG, anti-dsDNA and IL-6 protein levels induced by IL-17 was dose-dependent and could be completely blocked by IL-17 monoclonal antibody mIgG 28 and partially blocked by dexamethasone Similarly, IL-6 mRNA overexpression of PBMC in LN patients or normal controls induced by IL-17 was both dose- and time-dependent During medium culture, IL-6 mRNA levels in LN patients were significantly higher than those in normal controls (1 80±0 11 vs 0 36±0 07) During stimulation with IL-17, IL-6 mRNA levels in LN patients were higher than those in normal controls (3 21±0 24 vs 1 30±0 14, P<0 05) and also significantly higher when comparing the stimulated culture with the medium culture either in LN patients or normal control Conclusions IL-17 may play an important role in the pathogenesis of LN through the induction of IgG, anti-dsDNA overproduction and IL-6 overexpression of PBMC in LN patients
文摘Interleukin 6(IL-6) is known as hybridoma cell growth factor,B-cell differentiating factor and so on One of its important biological functions in to induce
