The Impact of Different Stain Carriers on the mRNA Profiling from Bloodstains

Unlike DNA profiling,mRNA profiling is greatly affected by external factors.To analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains,this study examined 10 carriers,including a knife,cotton swab,paper,plastic,leather,cement,chopsticks,clothes,ceramic block,and wall.After detecting five specific mRNA markers(HBA,HBB,ALAS2,GYPA,and SPTB)and the housekeeping gene B2M in peripheral blood samples,no statistically significant differences in the effects of the carriers were found.The results suggest that when performing mRNA testing on bloodstains,the effect of the stain carrier has little influence.

莎士比亚与英语词汇(14)

27.bloodstained(形容词)该词的意思是“血染的,有血迹的”。英语的释义是marked with blood;red with bolld.在莎士比亚较早的悲剧Titus Andronicus(《泰特斯·安德洛尼克斯》)中,罗马大将Andronicus的两个儿子Martius和Quintus在打猎途中遭人暗算,Mantius掉进一个深坑,坑里藏匿着刚刚被暗杀的Bassianus的尸体。Martius在坑里大喊:

Discussion on the Improvement of Management Mode of Student Status in Colleges and Universities

In order to improve the management of university status in China,it is necessary to actively transform the student status management model,it should be student service,and it’s turned into restraint and motivation,the mode of student service as a supplement is transformed.Under the model of service and constraint and incentive,we should strengthen the current management mode of student status in colleges and universities.And under the new model,the data quality of student status can be guaranteed,so that students’academic safety and education and teaching order can be improved accordingly,establish a good learning atmosphere,strengthening management.This paper analyzes and probes into the problems existing in the management of university status in China,this paper expounds the main contents of university student status management,and put forward the effective measures to improve the current management of university status.

Raman Spectroscopy for Forensic Identification of Body Fluid Traces: Method Validation for Potential False Negatives Caused by Blood-Affecting Diseases

Two critical issues in forensic science are identifying body fluid traces found at crime scenes and preserving them for DNA analysis. However, the majority of current biochemical tests for body fluid identification, which are applicable at the crime scene, are presumptive and destructive to the sample. Raman Spectroscopy provides a suitable alternative to current methods as a nondestructive, confirmatory, and potentially in field method. Our laboratory has developed a chemometric model for the identification of five main body fluids using Raman spectroscopy. This model was developed using samples obtained from healthy donors. Thus, it is of most importance for the forensic application of the method to validate its performance for donors with diseases that might affect the biochemical composition of body fluids. In this study, the developed method was validated using peripheral blood samples acquired from donors with Celiac Disease, Sickle Cell Anemia, and Type 2 Diabetes. It was shown that the method correctly identified all samples as peripheral blood indicating that no false positives could occur because the blood traces were originated from donors suffering from the diseases.

Discovering ester and ether derivatives of luminol as advanced chemiluminescence probes

A variety of luminol derivatives with N-and O-substitution have been synthesized with broad functional group tolerance. O-esterification has been demonstrated for the first time as a promising way to prepare enhanced CL reagents for sensing hemin, bloodstain and horseradish peroxidase(HRP). The most effective analogue with a deuterated acetyl group exhibited greater potential than luminol for bloodstain imaging and HRP imaging in western blotting(WB). In addition, O-etherification can greatly suppress CL signal that has been applied to design a CL probe for β-glucosidase(β-Glu). This study offers important and useful information regarding the luminol modification and shows great potential to use O-substituted analogues for enhanced CL analysis.