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A Comparison Between Northern Blotting and Quantitative Real-Time PCR as a Means of Detecting the Nutritional Regulation of Genes Expressed in Roots of Arabidopsis thaliana 被引量:4
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作者 GAN Yin-bo ZHOU Zhong-jing +2 位作者 AN Li-jun BAO Sheng-jie Brian G Forde 《Agricultural Sciences in China》 CAS CSCD 2011年第3期335-342,共8页
Quantitative real-time PCR (qRT-PCR) has become a routine and robust technique for measuring the expression of genes of interest, validating microarray experiments and monitoring biomarkers. However, concerns have b... Quantitative real-time PCR (qRT-PCR) has become a routine and robust technique for measuring the expression of genes of interest, validating microarray experiments and monitoring biomarkers. However, concerns have been raised over the accuracy of qRT-PCR in China as well as in the rest of the world. We have previously used qRT-PCR to study the response of ANR1 and other root-expressed MADS-box genes to fluctuations in the supply of nitrate, phosphate and sulphate under hydroponic growth conditions. In this study, we have used both Northern blotting and qRT-PCR analyses to confirm the nutritional regulation of MADS-box genes in Arabidopsis thaliana and test whether both technologies produce the same results. The information obtained indicated that the qRT-PCR results are consistent with those obtained by Northern blotting hybridization for all the tested root-expressed MADS-box genes, in response to different nitrate, phosphate and sulphate growth conditions. Furthermore, our novel results showed that the expressions of AGL12, AGL18, and AGL19 were all down regulated in response to S and P re-supply in both qRT-PCR and Northern blotting analyses. 展开更多
关键词 Arabidopsis thaliana MADS-BOX nutrient regulation northern blotting quantitative real-time PCR
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反向 Northern Blotting 对绵羊卵巢组织差异表达ESTs的鉴定
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作者 朱靖 杨娜娜 +1 位作者 柳淑芳 杜立新 《生物技术通报》 CAS CSCD 2005年第2期44-46,共3页
利用地高辛DNA标记与检测技术反向Northern斑点杂交,对小尾寒羊和滩羊卵巢组织差异显示ESTs进行鉴定,避免同位素放射性污染,安全性很高且操作简单,提高了实验的准确性,一种鉴定差异显示ESTs简单而有效的方法。在研究基因表达、比较不同... 利用地高辛DNA标记与检测技术反向Northern斑点杂交,对小尾寒羊和滩羊卵巢组织差异显示ESTs进行鉴定,避免同位素放射性污染,安全性很高且操作简单,提高了实验的准确性,一种鉴定差异显示ESTs简单而有效的方法。在研究基因表达、比较不同环境条件下动物组织的mRNA差异表达等方面具有广阔的应用前景。 展开更多
关键词 northern ESTS 差异表达 鉴定 反向 卵巢 绵羊 差异显示 DNA标记 放射性污染 斑点杂交 检测技术 小尾寒羊 基因表达 mRNA 动物组织 环境条件 地高辛 同位素 安全性 准确性
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Satellite observations of coastal upwelling in the northern Arafura Sea
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作者 Zheyuan YU Zhanjiu HAO +4 位作者 Zhenhua XU Peiwen ZHANG Jia YOU Baoshu YIN Hongwei YANG 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第2期361-376,共16页
Coastal upwelling is significant for marine ecosystems by lifting nutrient-rich deep waters into the euphotic zone,thereby increasing primary and secondary productivity.The satellite observations show that the norther... Coastal upwelling is significant for marine ecosystems by lifting nutrient-rich deep waters into the euphotic zone,thereby increasing primary and secondary productivity.The satellite observations show that the northern Arafura Sea(NAS),especially in the coastal region,features high chlorophyll-a(chl-a)concentrations,implying a strong coastal upwelling.However,coastal upwelling in the NAS has not received much attention.Based on a semi-automatic image processing technology,the seasonal and interannual variability of coastal upwelling in the NAS are investigated in this study using satellite-observed sea surface temperature(SST)and wind data.The results suggest that there are seasonal coastal upwelling events in the NAS modulated by upwelling-favorable southeast monsoon(SEM).The annual mean days,mean area,and annual mean intensity of coastal upwelling events during the SEM season are 92 days,6514 km^(2),and-5.31×10^(5),respectively,while the corresponding values during the northwest monsoon(NWM)season are 32 days,5569 km^(2),and-1.41×10^(5).It is also found that the SEM coastal upwelling in the NAS displays prominent interannual variability.The strong upwelling events are found in 2010,2013,2016,and 2017 when the southeast monsoon winds were weaker.Further analysis suggests that at the interannual scale,the upwelling index(UI)averaged in the SEM season is negatively correlated with that of three upwelling indicators.This can be attributed to the limitation of onshore geostrophic flow which is evidenced by the negative correlation between the UI and the alongshore difference in sea surface height.This study highlights the important role of the southeast monsoon in the temporal variability of coastal upwelling in the NAS. 展开更多
关键词 coastal upwelling the northern Arafura Sea southeast monsoon interannual variability
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Urban Spatial Form Optimization Strategies Based on the Principle of Climate Adaptability of Regional Architecture: A Case Study of Traditional Cave Dwelling Villages in Northern Shaanxi Province
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作者 QI Zizhuo YANG Xin 《Journal of Landscape Research》 2024年第3期1-7,共7页
A multitude of climate-adaptive design approaches are embedded in regional architecture,which have a positive impact on addressing the deformed development of urban spatial patterns and the dual challenges of climate ... A multitude of climate-adaptive design approaches are embedded in regional architecture,which have a positive impact on addressing the deformed development of urban spatial patterns and the dual challenges of climate and resources.This paper examines the principles of climate adaptability embedded in the site layout and spatial organization characteristics of traditional cave dwelling villages in northern Shaanxi Province.The extracted climate adaptability principles are summarized,and the resulting design strategies that are well-suited to the contemporary urban space form are presented.Through analysis,it can be observed that traditional cave dwelling villages in northern Shaanxi are predominantly situated on south-facing slopes in proximity to water at low altitudes.These villages are characterized by compact building groups and east-west development,which is constrained by the elements of mountains and rivers.A two-level street system is generated,comprising streets parallel to the contour line and roadways perpendicular to the contour line.This results in the formation of a courtyard form enclosed by mountains.Such site layout and spatial organization exhibit excellent climate adaptability with regard to heat,ventilation,and wind storage.In light of the aforementioned considerations,the following urban spatial form design strategies are put forth:①the topographic height difference can be exploited to obtain sufficient sunshine;②the group shape can be optimized in order to reduce building energy consumption;③the best orientation of the building can be chosen to take account of both winter and summer conditions;④the height and length can be combined in order to form natural masking;⑤the D/H ratio of streets and roadways should be controlled to achieve a balance between heat gain and cooling of groups;⑥vents should be set appropriately to optimize group ventilation;⑦climate buffers should be established to increase the level of climate response. 展开更多
关键词 Traditional cave dwellings in northern Shaanxi Urban spatial form Outdoor thermal environment
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反向Northern Blotting对小梅山猪下丘脑发育差异表达ESTs的鉴定 被引量:1
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作者 周春宝 潘孝青 +1 位作者 李霖 丁家桐 《江苏农业科学》 CSCD 北大核心 2009年第3期22-24,共3页
用mRNA差异显示技术从小梅山猪下丘脑不同发育阶段,分离并筛选出3个差异表达ESTs,分别命名为MS01、MS02、MS03。利用地高辛DNA标记与检测技术反向Northern斑点杂交,对分离出来的ESTs进行鉴定结果理想。此法避免了同位素放射性污染,操作... 用mRNA差异显示技术从小梅山猪下丘脑不同发育阶段,分离并筛选出3个差异表达ESTs,分别命名为MS01、MS02、MS03。利用地高辛DNA标记与检测技术反向Northern斑点杂交,对分离出来的ESTs进行鉴定结果理想。此法避免了同位素放射性污染,操作简单,可防止DDRT-PCR过程中假阳性的出现,提高了试验准确性,是一种鉴定差异显示ESTs简单而有效的方法。 展开更多
关键词 小梅山猪 反向northern blotting 下丘脑 ESTS 鉴定
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Evaluation of Medical Prescribers’ Theoretical Knowledge on Medical Imaging in the Northern Region of Burkina Faso
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作者 Some Milckisédek Judicaël Marouruana Ouedraogo Pakisba Ali +5 位作者 Tankoano Aïda Ida Savadogo Habibou Kindo Bassirou Ouedraogo Nina-Astrid Bamouni Yomboé Abel Diallo Ousseini 《Open Journal of Radiology》 2024年第2期33-41,共9页
Introduction: Medical imaging is a medical specialty that involves producing images of the human body and interpreting them for diagnostic, therapeutic purposes, and for monitoring the progress of pathologies. We aime... Introduction: Medical imaging is a medical specialty that involves producing images of the human body and interpreting them for diagnostic, therapeutic purposes, and for monitoring the progress of pathologies. We aimed to assess the theoretical knowledge of doctors and interns in medical imaging in the northern region of Burkina Faso. Methodology: This was a descriptive cross-sectional survey based on a self-administered questionnaire. Prescribers knowledge was estimated based on scores derived from questionnaire responses. Results: We collected 106 questionnaires out of 163, i.e. a participation rate of 65.03%. The average knowledge score was 81.71% for the contribution of medical imaging to patient management. It was 60.02% for the indications/counter-indications of radiological examinations and 72.56% for the risks associated with exposure to radiation during these examinations. The score was 59.83% for the methods used to select the appropriate radiological examination. As regards the completeness of the clinical and biological information on the forms requesting imaging examinations, the score was 96.65%. Specialist doctors had the highest overall level of knowledge (74.68%). Conclusion: Improved technical facilities, good initial and in-service training, and interdisciplinary collaboration will help to ensure that imaging tests are properly prescribed, leading to better patient care. 展开更多
关键词 Theoretical Knowledge Medical Imaging northern Region Burkina Faso
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Effects of Enclosure on Grassland Ecological and Economic Benefits in Northern China
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作者 HU Bo WEN Qingke +3 位作者 XI Fengjiang LI Mengyao WANG Libing REN Yuejuan 《Chinese Geographical Science》 SCIE CSCD 2024年第1期67-78,共12页
Grasslands in northern China serve the country as both an ecological barrier and a livestock production base.There,installing enclosures has been becoming the major grassland restoration measure adopted by many local ... Grasslands in northern China serve the country as both an ecological barrier and a livestock production base.There,installing enclosures has been becoming the major grassland restoration measure adopted by many local governments.However,the effects of restoration on both ecological and production benefits of grassland remain unclear for implemented grassland restoration policies.Therefore,a representative rangeland in northern China,the Maodeng pasture in Inner Mongolia Autonomous Region was selected as the study area,and remote sensing monitoring analyses were carried out to quantify the ecological benefits and economic benefits from 2015 to 2021.The results showed that:1) in terms of ecological benefits,the grassland area with a grassland coverage rate of more than 60% accounts for 32.3% of the regional area,and 86.4% of its grassland grew significantly better than the same period in2015,showing a significant improvement in grassland growth.Using the average amount of carbon per unit area as the ecological benefit evaluation index,it increased by 27.1% to 32.48Tg C/yr from 2015 to 2021.2) In terms of economic benefits,both theoretical grass production and livestock carrying capacity increased from 2015 to 2021.Compared to 2015,the theoretical grass production in 2021 increased by 24.8% to 71 900 t.The livestock carrying capacity reached 52 100 sheep units in 2021,nearly 11 000 sheep units more than that in 2015.During the study period,multiple economic indicators(on a per capita basis of permanent residents) for the pastoral area of Xilinhot City to which the Maodeng pasture belongs,have grown steadily.Per capita total income rose from 29 630 yuan(RMB) in2015 to 62 859 yuan(RMB) in 2021.Relying on grassland resources to develop the pastoral ecology also broadens the potential economic development space.Overall,the establishment of the reserve and the experiment of implanting an enclosure policy have had a significant and positive impact on Maodeng pasture’s development from both an ecological and economic perspective.With the support of scientific evidence,enclosure policy can be extended to more than 110 000 km~2 of grasslands in northern China with similar precipitation and temperature conditions,enhancing the productive and ecological potential of grasslands.The above research results will contribute to the scientific formulation of grassland pasture quality improvement plans in northern China. 展开更多
关键词 ENCLOSURE GRASSLAND ecological benefit economic benefit Maodeng pasture Inner Mongolia northern China
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Coral records of Mid-Holocene sea-level highstands and climate responses in the northern South China Sea
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作者 Yuanfu Yue Lichao Tang +1 位作者 Kefu Yu Rongyong Huang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2024年第2期43-57,共15页
High-resolution sea-level data and high-precision dating of corals in the northern South China Sea(SCS)during the Holocene provide a reference and historical background for current and future sea-level changes and a b... High-resolution sea-level data and high-precision dating of corals in the northern South China Sea(SCS)during the Holocene provide a reference and historical background for current and future sea-level changes and a basis for scientific assessment of the evolutionary trend of coral reefs in the SCS.Although sporadic studies have been performed around Hainan Island in the northern SCS,the reconstructed sea level presents different values or is controversial because the indicative meaning of the sea-level indicators were neither quantified nor uniform criteria.Here,we determined the quantitative relationship between modern living coral and sea level by measuring the top surfaces of 27 live Porites corals from the inner reef flat along the east coast of Hainan Island and assessed the accuracy of results obtained using coral as sea-level indicators.Additionally,three in situ fossil Porites corals were analyzed based on elevation measurements,digital X-ray radiography,and U-Th dating.The survey results showed that the indicative meanings for the modern live Porites corals is(146.09±8.35)cm below the mean tide level(MTL).It suggested that their upward growth limit is constrained by the sea level,and the lowest low water is the highest level of survival for the modern live Porites corals.Based on the newly defined indicative meanings,6 new sea-level index points(SLIPs)were obtained and 19 published SLIPs were recalculated.Those SLIPs indicated a relative sea level fluctuation between(227.7±9.8)cm to(154.88±9.8)cm MTL between(5393±25)cal a BP and(3390±12)cal a BP,providing evidences of the Mid-Holocene sea-level highstand in the northern SCS.Besides that,our analysis demonstrated that different sea-level histories may be produced based on different indicative meanings or criteria.The dataset of 276 coral U-Th ages indicates that coral reef development in the northern SCS comprised the initial development,boom growth,decline,and flourishing development again.A comparison with regional records indicated that synergistic effects of climatic and environmental factors were involved in the development of coral reefs in the northern SCS.Thus,the cessation of coral reef development during the Holocene in the northern SCS was probably associated with the dry and cold climate in South China,as reflected in the synchronous weakening of the ENSO and East Asian summer monsoon induced by the reduction of the 65°N summer insolation,which forced the migration of the Intertropical Convergence Zone. 展开更多
关键词 northern South China Sea Middle Holocene sea-level highstand Porites corals climate response
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Analysis on characteristics of extreme precipitation indices and atmospheric circulation in Northern Shanxi
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作者 Xia Cai Yan Song +3 位作者 Lin Cai Xin Su GuiHua Liang YanMing Xu 《Research in Cold and Arid Regions》 CSCD 2024年第2期84-97,共14页
This article utilizes daily precipitation data from 28 national meteorological stations in northern Shanxi Province spanning from 1972 to 2020,and the US NCEP/NCAR monthly average reanalysis and ERA5 monthly average r... This article utilizes daily precipitation data from 28 national meteorological stations in northern Shanxi Province spanning from 1972 to 2020,and the US NCEP/NCAR monthly average reanalysis and ERA5 monthly average reanalysis data.The study employs techniques such as empirical orthogonal function(EOF)decomposition,MannKendall mutation and other methods to investigate the spatiotemporal distribution of extreme precipitation index in northern Shanxi and their correlation with atmospheric circulation.The research results show that:the absolute index,relative index,intensity index and sustained dry period index(CDD)in the continuous index appear from southwest to northeast.The spatial distribution characteristics of the central region decrease,while the continuous wet period(CWD)decreases from the central to the east and west.The three indices Rx1day,Rx5day,and CWD mutated in 1978,1975,and 1983 respectively,and other extreme precipitation indices all appeared in a sudden change from a low-value period to a high-value period occurred around 2010.In the high-value years of the summer extreme precipitation index,there is a significant negative anomaly in the height field in the mid-high latitude regions of Eurasia.Northern Shanxi is controlled by a broad low-pressure trough in the Lake Baikal area.Water vapor transported via the east,west,and south routes converges in the northern Shanxi region and encounters cold air from the north.There is a strong upward motion anomaly at 500 hPa in the troposphere,and the dynamic conditions of upper-level divergence and lower-level convergence lead to more summer extreme precipitation in the northern Shanxi region.Conversely,in the low-value years of the summer extreme precipitation index,northern Shanxi is affected by a strong high-pressure ridge north of Lake Baikal.There is a downward motion anomaly at 500 hPa,and the northern Shanxi region lacks water vapor.The cold and warm air cannot converge,and both the water vapor conditions and dynamic conditions are poor,which is not conducive to the production of extreme precipitation in northern Shanxi. 展开更多
关键词 northern Shanxi Extreme precipitation indices Spatio-temporal distribution and evolution MUTATIONS Atmospheric circulation anomaly
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A Multi-Strategy-Improved Northern Goshawk Optimization Algorithm for Global Optimization and Engineering Design
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作者 Liang Zeng Mai Hu +2 位作者 Chenning Zhang Quan Yuan Shanshan Wang 《Computers, Materials & Continua》 SCIE EI 2024年第7期1677-1709,共33页
Optimization algorithms play a pivotal role in enhancing the performance and efficiency of systems across various scientific and engineering disciplines.To enhance the performance and alleviate the limitations of the ... Optimization algorithms play a pivotal role in enhancing the performance and efficiency of systems across various scientific and engineering disciplines.To enhance the performance and alleviate the limitations of the Northern Goshawk Optimization(NGO)algorithm,particularly its tendency towards premature convergence and entrapment in local optima during function optimization processes,this study introduces an advanced Improved Northern Goshawk Optimization(INGO)algorithm.This algorithm incorporates a multifaceted enhancement strategy to boost operational efficiency.Initially,a tent chaotic map is employed in the initialization phase to generate a diverse initial population,providing high-quality feasible solutions.Subsequently,after the first phase of the NGO’s iterative process,a whale fall strategy is introduced to prevent premature convergence into local optima.This is followed by the integration of T-distributionmutation strategies and the State Transition Algorithm(STA)after the second phase of the NGO,achieving a balanced synergy between the algorithm’s exploitation and exploration.This research evaluates the performance of INGO using 23 benchmark functions alongside the IEEE CEC 2017 benchmark functions,accompanied by a statistical analysis of the results.The experimental outcomes demonstrate INGO’s superior achievements in function optimization tasks.Furthermore,its applicability in solving engineering design problems was verified through simulations on Unmanned Aerial Vehicle(UAV)trajectory planning issues,establishing INGO’s capability in addressing complex optimization challenges. 展开更多
关键词 northern Goshawk Optimization tent chaotic map T-distribution disturbance state transition algorithm UAV path planning
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Depression&Anxiety Among Women in Northern Kenya:Sociodemographic Predictors
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作者 Gladys Kabura Mwangi 《Psychology Research》 2024年第2期57-63,共7页
This article documents socio-demographic predictors of anxiety and depression among Maasai women in resource poor settings in Northern Kenya.These findings emerged from the findings of a dissertation research that aim... This article documents socio-demographic predictors of anxiety and depression among Maasai women in resource poor settings in Northern Kenya.These findings emerged from the findings of a dissertation research that aimed to establish the treatment effectiveness of an eclectic model of psychoeducation(PE)in treating depressive and anxiety symptoms among the women.The study adopted a quasi-experimental research design which had an experimental group(EG)and control group(CG).The population for the study was 686 female members of Conservation Enterprise Groups(CEG)in Laikipia County,from which a sample of 200 were recruited for the study(EG,n=100 and CG,n=100),at 80%power and 30%effective size.The Beck’s Depression Inventory(BDI)and Beck’s Anxiety Inventory(BAI)tools were used to assess the symptoms of depression and anxiety,respectively.The PE was provided as an intervention treatment to the EG at the middle and end of the study,after which the respondents were tested for symptoms of depression.The study established the prevalence of depression as 26.7%and 31.3%for anxiety and disorders,among women in the region.Data was analyzed using SPSS Version 21.0. 展开更多
关键词 SOCIODEMOGRAPHIC ANXIETY DEPRESSION PREDICTORS Maasai women northern Kenya
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Spatial and Temporal Analysis of Maximum and Minimum Temperature Trends in Northern Sudan during (1990-2019)
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作者 Elhag Gamreldin Monzer Hamadalnel 《Journal of Geoscience and Environment Protection》 2024年第5期266-288,共23页
The study addresses an urgent and globally significant issue of climate change by focusing on the detailed spatial and temporal analysis of temperature trends in Northern Sudan. It fills a critical research gap by pro... The study addresses an urgent and globally significant issue of climate change by focusing on the detailed spatial and temporal analysis of temperature trends in Northern Sudan. It fills a critical research gap by providing localized data over a substantial period (1990-2019), which could help in understanding the nuanced impacts of climate change in Sahel regions like Northern Sudan. In addition, the comprehensive coverage of both spatial and temporal dimensions, supported by a substantial dataset from five meteorological stations, provides a thorough understanding of the subject area. The utilization of robust statistical methods (Mann-Kendall test and Sen’s slope analysis) for analyzing temperature trends adds scientific rigor and credibility to the findings. Our results reveal a consistently increasing trend in maximum temperatures across most stations, particularly during the hot season (AMJ). However, the wet season (JAS) shows high maximum temperatures but no significant trend. Moreover, significant increasing trends in minimum temperatures were observed in all stations except Abu Hamed, where the trend, although increasing, did not reach statistical significance during the hot and cold seasons, and the coldest temperatures were observed during the cold season. These findings underscore the complex temperature dynamics in Northern Sudan and highlight the need for continued monitoring and adaptive measures in response to ongoing climate changes in the region. 展开更多
关键词 Climate Change northern Sudan Temperature Trend Seasonal Analysis
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Spatial differences of Sustainable Development Goals(SDGs)among counties(cities)on the northern slope of the Kunlun Mountains
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作者 WANG Tao ZHOU Daojing FAN Jie 《Regional Sustainability》 2024年第1期1-10,共10页
The county(city)located on the northern slope of the Kunlun Mountains is the primary area to solidify and extend the success of Xinjiang Uygur Autonomous Region,China in poverty alleviation.Its Sustainable Development... The county(city)located on the northern slope of the Kunlun Mountains is the primary area to solidify and extend the success of Xinjiang Uygur Autonomous Region,China in poverty alleviation.Its Sustainable Development Goals(SDGs)are intertwined with the concerted economic and social development of Xinjiang and the objective of achieving shared prosperity within the region.This study established a sustainable development evaluation framework by selecting 15 SDGs and 20 secondary indicators from the United Nations’SDGs.The aim of this study is to quantitatively assess the progress of SDGs at the county(city)level on the northern slope of the Kunlun Mountains.The results indicate that there are substantial variations in the scores of SDGs among the nine counties and one city located on the northern slope of the Kunlun Mountains.Notable high scores of SDGs are observed in the central and eastern regions,whereas lower scores are prevalent in the western areas.The scores of SDGs,in descending order,are as follows:62.22 for Minfeng County,54.22 for Hotan City,50.21 for Qiemo County,42.54 for Moyu County,41.56 for Ruoqiang County,41.39 for Qira County,39.86 for Lop County,38.25 for Yutian County,38.10 for Pishan County,and 36.87 for Hotan County.The performances of SDGs reveal that Hotan City,Lop County,Minfeng County,and Ruoqiang County have significant sustainable development capacity because they have three or more SDGs ranked as green color.However,Hotan County,Moyu County,Qira County,and Yutian County show the poorest performance,as they lack SDGs with green color.It is important to establish and enhance mechanisms that can ensure sustained income growth among poverty alleviation beneficiaries,sustained improvement in the capacity of rural governance,and the gradual improvement of social security system.These measures will facilitate the effective implementation of SDGs.Finally,this study offers a valuable support for governmental authorities and relevant departments in their decision-making processes.In addition,these results hold significant reference value for assessing SDGs at the county(city)level,particularly in areas characterized by low levels of economic development. 展开更多
关键词 SUSTAINABLE Development Goals(SDGs) northern slope of the Kunlun Mountains Poverty alleviation Arid lands SUSTAINABLE development capacity
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Enrichment model and major controlling factors of below-source tight oil in Lower Cretaceous Fuyu reservoirs in northern Songliao Basin,NE China
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作者 WANG Xiaojun BAI Xuefeng +9 位作者 LI Junhui JIN Zhijun WANG Guiwen CHEN Fangju ZHENG Qiang HOU Yanping YANG Qingjie LI Jie LI Junwen CAI Yu 《Petroleum Exploration and Development》 SCIE 2024年第2期279-291,共13页
Based on the geochemical,seismic,logging and drilling data,the Fuyu reservoirs of the Lower Cretaceous Quantou Formation in northern Songliao Basin are systematically studied in terms of the geological characteristics... Based on the geochemical,seismic,logging and drilling data,the Fuyu reservoirs of the Lower Cretaceous Quantou Formation in northern Songliao Basin are systematically studied in terms of the geological characteristics,the tight oil enrichment model and its major controlling factors.First,the Quantou Formation is overlaid by high-quality source rocks of the Upper Cretaceous Qingshankou Formation,with the development of nose structure around sag and the broad and continuous distribution of sand bodies.The reservoirs are tight on the whole.Second,the configuration of multiple elements,such as high-quality source rocks,reservoir rocks,fault,overpressure and structure,controls the tight oil enrichment in the Fuyu reservoirs.The source-reservoir combination controls the tight oil distribution pattern.The pressure difference between source and reservoir drives the charging of tight oil.The fault-sandbody transport system determines the migration and accumulation of oil and gas.The positive structure is the favorable place for tight oil enrichment,and the fault-horst zone is the key part of syncline area for tight oil exploration.Third,based on the source-reservoir relationship,transport mode,accumulation dynamics and other elements,three tight oil enrichment models are recognized in the Fuyu reservoirs:(1)vertical or lateral migration of hydrocarbon from source rocks to adjacent reservoir rocks,that is,driven by overpressure,hydrocarbon generated is migrated vertically or laterally to and accumulates in the adjacent reservoir rocks;(2)transport of hydrocarbon through faults between separated source and reservoirs,that is,driven by overpressure,hydrocarbon migrates downward through faults to the sandbodies that are separated from the source rocks;and(3)migration of hydrocarbon through faults and sandbodies between separated source and reservoirs,that is,driven by overpressure,hydrocarbon migrates downwards through faults to the reservoir rocks that are separated from the source rocks,and then migrates laterally through sandbodies.Fourth,the differences in oil source conditions,charging drive,fault distribution,sandbody and reservoir physical properties cause the differential enrichment of tight oil in the Fuyu reservoirs.Comprehensive analysis suggests that the Fuyu reservoir in the Qijia-Gulong Sag has good conditions for tight oil enrichment and has been less explored,and it is an important new zone for tight oil exploration in the future. 展开更多
关键词 northern Songliao Basin Cretaceous Quantou Formation Qingshankou Formation upper generation and lower storage Fuyu reservoir tight oil main control factor enrichment model
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Trend in seasonal amplitude of northern net ecosystem production:Simulated results from IAP DGVM in CAS-ESM2
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作者 Jiawen Zhu Xiaodong Zeng 《Atmospheric and Oceanic Science Letters》 CSCD 2024年第2期45-51,共7页
北方陆地净生态系统生产力(NEP)具有明显的季节变化特征,这是大气CO_(2)季节变化的关键驱动.研究这些碳循环过程并理解潜在的驱动因素是气候研究的一个关键问题.本文利用第二代中国科学院地球系统模式(CAS-ESM2)中的全球植被动态模型(IA... 北方陆地净生态系统生产力(NEP)具有明显的季节变化特征,这是大气CO_(2)季节变化的关键驱动.研究这些碳循环过程并理解潜在的驱动因素是气候研究的一个关键问题.本文利用第二代中国科学院地球系统模式(CAS-ESM2)中的全球植被动态模型(IAPDGVM),研究了1990-2014年北方NEP(40°-90°N)的季节振幅及其变化趋势.在初始化试验的基础上,本文开展了一个控制试验来评估模拟的北方NEP季节幅度的变化趋势,同时开展了三个敏感性试验来研究气候和大气CO_(2)的贡献.结果表明:1990-2014年,模拟的北方NEP季节振幅显著增加,趋势为9.69万吨碳/月/年,这主要是由于最大NEP增加所致.当分别排除CO_(2)施肥效应和气候效应时,上述增加趋势大大减弱.这些显著的减少表明大气CO_(2)和气候变化对北方NEP的季节性振幅有重要影响.尽管模式存在不确定性,但这些结果有利于进一步提升IAPDGVM对陆地碳循环的精确模拟,也为CAS-ESM研究碳-气候相互作用的应用提供了重要参考. 展开更多
关键词 净生态系统生产力 北方陆地生态系统 季节变化幅度 CO_(2)施肥效应 气候效应
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低温诱导甜菜(Beta vulgaris L.)Ty7Br600基因的Northern blotting和Southern blotting分析 被引量:1
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作者 戴建军 程大友 +3 位作者 常缨 李彩凤 闫桂萍 马凤鸣 《东北农业大学学报》 CAS CSCD 北大核心 2012年第4期80-83,共4页
以低温诱导甜菜幼苗为试验材料,以未进行低温诱导的甜菜幼苗为对照,采用α-32P-ATP放射标记按随机引物标记法标记本实验室克隆的Ty7Br600基因,进行Northern杂交。Northern杂交试验结果显示,在低温诱导培养的甜菜幼苗的RNA群体中,出现较... 以低温诱导甜菜幼苗为试验材料,以未进行低温诱导的甜菜幼苗为对照,采用α-32P-ATP放射标记按随机引物标记法标记本实验室克隆的Ty7Br600基因,进行Northern杂交。Northern杂交试验结果显示,在低温诱导培养的甜菜幼苗的RNA群体中,出现较强的杂交条带,而在未诱导培养的甜菜幼苗的RNA群体中,则几乎没有阳性杂交条带出现。因此,Ty7Br600这一序列有可能是二年生甜菜幼苗经低温诱导处理之后被诱导表达的与抽薹相关的新基因序列。Southern杂交结果发现,在每一组酶切中至少有2~3条条带,表明Ty7Br600确为甜菜基因组片段,也同时表明该基因在甜菜基因组中以2个拷贝或低拷贝形式存在。 展开更多
关键词 甜菜(Beta VULGARIS L.) 低温诱导 northern杂交 SOUTHERN杂交
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Clay minerals and elemental composition of sediments on different sedimentary units in the northern East China Sea shelf:provenance tracing and genetic mechanism analysis
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作者 Xiaoyan Xu Yong Zhang +6 位作者 Yanguang Dou Jingyi Cong Beibei Mi Xiaohui Chen Xia Li Chengfen Xu Yongyu Ye 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第11期19-34,共16页
The composition,provenance,and genetic mechanism of sediment on different sedimentary units of the East China Sea(ECS)shelf are essential for understanding the depositional dynamics environment in the ECS.The sediment... The composition,provenance,and genetic mechanism of sediment on different sedimentary units of the East China Sea(ECS)shelf are essential for understanding the depositional dynamics environment in the ECS.The sediments in the northern ECS shelf are distributed in a ring-shaped distribution centered on the southwestern Cheju Island Mud.From the inside to the outside,the grain size goes from fine to coarse.Aside from the“grain size effect”,hydrodynamic sorting and mineral composition are important restrictions on the content of rare earth elements(REEs).Based on the grain size,REEs,and clay mineral composition of 300 surface sediments,as well as the sedimentary genesis,the northern ECS shelf is divided into three geochemical zones:southwestern Cheju Island Mud Area(ZoneⅠ),Changjiang Shoal Sand Ridges(ZoneⅡ-1),Sand Ridges of the East China Sea shelf(ZoneⅡ-2).The northern ECS shelf is mostly impacted by Chinese mainland rivers(the Changjiang River and Huanghe River),and the provenance and transport mechanism of sediments of different grain sizes is diverse.The bulk sediments come primarily from the Changjiang River,with some material from the Huanghe River carried by the Yellow Sea Coastal Current and the North Jiangsu Coastal Current,and less from Korean rivers.Among them,surface sediments in the southwestern Cheju Island Mud Area(ZoneⅠ)come mostly from the Changjiang River and partly from the Huanghe River.It was formed by the counterclockwise rotating cold eddies in the northern ECS shelf,which caused the sedimentation and accumulation of the fine-grained sediments of the Changjiang River and the Huanghe River.The Changjiang Shoal Sand Ridges(ZoneⅡ-1)were developed during the early-middle Holocene sea-level highstand.It is the modern tidal sand ridge sediment formed by intense hydrodynamic action under the influence of the Yellow Sea Coastal Current,North Jiangsu Coastal Current,and Changjiang Diluted Water.The surface sediments mainly originate from the Changjiang River and Huanghe River,with the Changjiang River dominating,and the Korean River(Hanjiang River)influencing just a few stations.Sand Ridges of the East China Sea shelf(ZoneⅡ-2)are the relict sediments of the paleo-Changjiang River created by sea invasion at the end of the Last Deglaciation in the Epipleistocene.The clay mineral composition of the surface sediments in the study area is just dominated by the Changjiang River,with the North Jiangsu Coastal Current and the Changjiang Diluted Water as the main transporting currents. 展开更多
关键词 the northern East China Sea shelf rare earth element clay mineral PROVENANCE genetic mechanism
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Study of the Spatio-Temporal Variability of Rainfall in the Northern Region of Senegal
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作者 Aichétou Dia Diop Abdoul Karim Mbodji +8 位作者 El Hadji Deme Malick Wade Mahamat Adoum Moussa Abdoulaye Bouya Diop Younousse Biaye Djiby Sarr Abdou Karim Farota Aboubakary Diakhaby Bouya Diop 《Journal of Geoscience and Environment Protection》 2023年第4期1-14,共14页
Senegal is a country of the Sahel. In this region, most of the populations live from agro-pastoral activities. The northern zone of Senegal is strongly influenced by river cultures. And the dynamics of the Senegal Riv... Senegal is a country of the Sahel. In this region, most of the populations live from agro-pastoral activities. The northern zone of Senegal is strongly influenced by river cultures. And the dynamics of the Senegal River are dependent on rainfall. The rainfall in the area is very closely linked to the dynamics of the atmosphere. The study of the spatio-temporal variability of rainfall in the northern region of Senegal requires quality rainfall observation data. It includes the Ferlo and the Senegal River valley, in particular the regions of Louga (department of Linguère included), Saint-Louis (departments of Dagana and Podor included) and Matam. These stations have been defined since Le Borgne (1988). The difficulty of having quality rain observation data can be resolved by using more accessible and good quality satellite data. Using satellite data, namely MSWEP, CRU, TAMSAT, ARC and PERSIANN, we showed the return of precipitation that appeared in 2000 and the unimodal cycle of precipitation in our study area. These data were validated using the correlation coefficient, the bias, the RMSE and the Nash index with observation data from the Regional Study Center for the Improvement of Adaptation to Drought (CERASS). The CRU data is then retained. Thus, this study made it possible to show the zonal distribution of rainfall in the northern zone of Senegal. 展开更多
关键词 Rainfall in the northern Region of Senegal Variability of Rainfall Satellite Data The Zonal Distribution of Rainfall
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Temporal and spatial responses of ecological resilience to climate change and human activities in the economic belt on the northern slope of the Tianshan Mountains, China
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作者 ZHANG Shubao LEI Jun +4 位作者 TONG Yanjun ZHANG Xiaolei LU Danni FAN Liqin DUAN Zuliang 《Journal of Arid Land》 SCIE CSCD 2023年第10期1245-1268,共24页
In the Anthropocene era,human activities have become increasingly complex and diversified.The natural ecosystems need higher ecological resilience to ensure regional sustainable development due to rapid urbanization a... In the Anthropocene era,human activities have become increasingly complex and diversified.The natural ecosystems need higher ecological resilience to ensure regional sustainable development due to rapid urbanization and industrialization as well as other intensified human activities,especially in arid and semi-arid areas.In the study,we chose the economic belt on the northern slope of the Tianshan Mountains(EBNSTM)in Xinjiang Uygur Autonomous Region of China as a case study.By collecting geographic data and statistical data from 2010 and 2020,we constructed an ecological resilience assessment model based on the ecosystem habitat quality(EHQ),ecosystem landscape stability(ELS),and ecosystem service value(ESV).Further,we analyzed the temporal and spatial variation characteristics of ecological resilience in the EBNSTM from 2010 to 2020 by spatial autocorrelation analysis,and explored its responses to climate change and human activities using the geographically weighted regression(GWR)model.The results showed that the ecological resilience of the EBNSTM was at a low level and increased from 0.2732 to 0.2773 during 2010–2020.The spatial autocorrelation analysis of ecological resilience exhibited a spatial heterogeneity characteristic of"high in the western region and low in the eastern region",and the spatial clustering trend was enhanced during the study period.Desert,Gobi and rapidly urbanized areas showed low level of ecological resilience,and oasis and mountain areas exhibited high level of ecological resilience.Climate factors had an important impact on ecological resilience.Specifically,average annual temperature and annual precipitation were the key climate factors that improved ecological resilience,while average annual evapotranspiration was the main factor that blocked ecological resilience.Among the human activity factors,the distance from the main road showed a negative correlation with ecological resilience.Both night light index and PM2.5 concentration were negatively correlated with ecological resilience in the areas with better ecological conditions,whereas in the areas with poorer ecological conditions,the correlations were positive.The research findings could provide a scientific reference for protecting the ecological environment and promoting the harmony and stability of the human-land relationship in arid and semi-arid areas. 展开更多
关键词 ecological resilience ecosystem habitat quality ecosystem landscape stability ecosystem service value spatial autocorrelation analysis geographically weighted regression model economic belt on the northern slope of the Tianshan Mountains
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The Possible Effect of Climate Warming on Northern Limits of Cropping System and Crop Yield in China 被引量:18
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作者 YANG Xiao-guang LIU Zhi-juan CHEN Fu 《Agricultural Sciences in China》 CAS CSCD 2011年第4期585-594,共10页
Significantly increasing temperature since the 1980s in China has become a consensus under the background of global climate change and how climate change affects agriculture or even cropping systems has attracted more... Significantly increasing temperature since the 1980s in China has become a consensus under the background of global climate change and how climate change affects agriculture or even cropping systems has attracted more and more attention from Chinese government and scientists. In this study, the possible effects of climate warming on the national northern limits of cropping systems, the northern limits of winter wheat and double rice, and the stable-yield northern limits of rainfed winter wheat-summer maize rotation in China from 1981 to 2007 were analyzed. Also, the possible change of crop yield caused by planting limits displacement during the periods 1950s-1981 and 1981-2007 was compared and discussed. The recognized calculation methods of agricultural climatic indices were employed. According to the indices of climatic regionalization for cropping systems, the national northern limits of cropping systems, winter wheat and double rice, and the stable-yield northern limits of rainfed winter wheat-summer maize rotation during two periods, including the 1950s-1980 and 1981-2007, were drawn with ArcGIS software. Compared with the situation during the 1950s- 1980, the northern limits of double cropping system during 1981-2007 showed significant spatial displacement in Shaanxi, Shanxi, Hebei, and Liaoning provinces and Beijing municipality, China. The northern limits of triple cropping system showed the maximum spatial displacement in Hunan, Hubei, Anhui, Jiangsu, and Zhejiang provinces, China. Without considering variety change and social economic factors, the per unit area grain yield of main planting patterns would increase about 54-106% if single cropping system was replaced by double cropping system, which turned out to be 27- 58% if double cropping system was replaced by triple cropping system. In Liaoning, Hebei, Shanxi, Shaanxi, Gansu, and Qinghai provinces, Inner Mongolia and Ningxia autonomous regions, China, the northern limits of winter wheat during 1981-2007 moved northward and expanded westward in different degrees, compared with those during the 1950s-1980. Taking Hebei Province as an example, the northern limits of winter wheat moved northward, and the per unit area grain yield would averagely increase about 25% in the change region if the spring wheat was replaced by winter wheat. In Zhejiang, Anhui, Hubei, and Hunan provinces, China, the planting northern limits of double rice moved northward, and the per unit area grain yield would increase in different degrees only from the perspective of heat resource. The stable- yield northern limits of rainfed winter wheat-summer maize rotation moved southeastward in most regions, which was caused by the decrease of local precipitation in recent years. During the past 50 yr, climate warming made the national northern limits of cropping systems move northward in different degrees, the northern limits of winter wheat and double rice both moved northward, and the cropping system change would cause the increase of per unit area grain yield in the change region. However, the stable-yield northern limits of rainfed winter wheat-summer maize rotation moved southeastward due to the decrease of precipitation. 展开更多
关键词 climate warming the northern limits of cropping system the planting northern limits of winter wheat the planting northern limits of double rice crop yield
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