Based on the turbulent convection model （TCM）, we investigate chemical mixing in the bottom overshooting region of the convective envelope of intermediatemass stars, focusing on its influence on the formation and ex...Based on the turbulent convection model （TCM）, we investigate chemical mixing in the bottom overshooting region of the convective envelope of intermediatemass stars, focusing on its influence on the formation and extension of blue loops in the Hertzsprung-Russell （HR） diagram. A diffusive mixing model is adopted during the Red Giant Branch （RGB） phase. The properties of the blue loop are changed by modification of the element profiles above the H-burning shell, which results from the incomplete mixing in the bottom overshooting region when the stellar model evolves up along the RGB. Such modification of the element profiles will lead to an increase of opacity in the region just above the H-burning shell and a decrease of opacity in the outer homogeneous convection zone, which will result in a quick decrease of the H-shell nuclear luminosity LH when the stellar model evolves from the RGB tip to its bottom and, finally, a much weaker and smaller convection zone will be obtained in the stellar envelope. This helps to form a longer blue loop. The extension of the blue loop is very sensitive to the parameters （Cx and αTCM） of the diffusive mixing model and of the TCM. The results mainly show that： 1） comparing the results of the classical model with the mixing-length theory, the lengths of the obtained blue loops with different combinations of the values of Cx and αTCM are all increased and the length of the blue loop increases with the values of parameters Cx and αTCM; 2） the diffusive mixing model can significantly extend the time of stellar models lingering on the blue side of the HR diagram, even though the length of the blue loop for the 7M⊙ star has a less prominent difference between the classical and diffusive mixing model; 3） both the observations referring to the location of the Cepheid instability strip and the number ratio NB/NR of blue to red evolved stars in the Galactic open clusters can confine the two parameters in a range of 0.5 ≤ αLTCM ≤ 0.9 and 10-5 ≤ Cx ≤ 10-4 for the model of 5M⊙. However, for the case of the 7M⊙ star, there seems to be no such definite range to even only account for the observed number ratio NB/NR. In any case, our results based on the diffusive mixing model are on the whole in accordance with not only other theoretical ones but also the observations.展开更多
建立一种荧光染料叠氮溴化丙锭(PMA)与环介导等温扩增(LAMP)相结合的技术,同时利用羟基萘酚蓝(HNB)染料对婴儿配方奶粉中沙门氏菌活菌进行快速可视化检测。以沙门氏菌siiA基因为靶点,设计特异性引物,利用PMA抑制死菌DNA的扩增反应,反应...建立一种荧光染料叠氮溴化丙锭(PMA)与环介导等温扩增(LAMP)相结合的技术,同时利用羟基萘酚蓝(HNB)染料对婴儿配方奶粉中沙门氏菌活菌进行快速可视化检测。以沙门氏菌siiA基因为靶点,设计特异性引物,利用PMA抑制死菌DNA的扩增反应,反应前向体系中加入HNB染料,通过颜色变化对目标菌进行快速检测。结果表明,PMA的最佳处理浓度为3μg/mL,体系中HNB的最优浓度为150μmol/L,所构建的PMA-LAMP-HNB方法对沙门氏菌纯培养物的检测灵敏度为4.6×10^1CFU/mL,对人工污染婴儿配方奶粉中该菌的检测灵敏度为6.3×10^1CFU/g,是传统PCR方法的100倍,且总检测时间不超过1 h 30 min。展开更多
文摘为更好地对长顺绿壳蛋鸡进行品种保护和利用,并从母系遗传角度深入阐明长顺绿壳蛋鸡的群体遗传背景,利用PCR直接测序法,测定了114只长顺绿壳蛋鸡的线粒体DNA(mt DNA)D-loop第1高变区568 bp片段序列。结果表明:在所分析的长顺绿壳蛋鸡mt DNA D-loop区序列中,A、C、G、T平均比例为27.1%、29.5%、13.4%、30.0%;共检测到核苷酸变异位点37个,核苷酸多样度为0.0111,24种单倍型,单倍型多样度为0.845,表明长顺绿壳蛋鸡遗传多样性较丰富,具有较好的选育潜力。聚类分析结果表明,长顺绿壳蛋鸡与红原鸡亲缘关系较近。
基金supported by the National Natural Science Foundation of China(Grant Nos.10973035 and 10673030)
文摘Based on the turbulent convection model （TCM）, we investigate chemical mixing in the bottom overshooting region of the convective envelope of intermediatemass stars, focusing on its influence on the formation and extension of blue loops in the Hertzsprung-Russell （HR） diagram. A diffusive mixing model is adopted during the Red Giant Branch （RGB） phase. The properties of the blue loop are changed by modification of the element profiles above the H-burning shell, which results from the incomplete mixing in the bottom overshooting region when the stellar model evolves up along the RGB. Such modification of the element profiles will lead to an increase of opacity in the region just above the H-burning shell and a decrease of opacity in the outer homogeneous convection zone, which will result in a quick decrease of the H-shell nuclear luminosity LH when the stellar model evolves from the RGB tip to its bottom and, finally, a much weaker and smaller convection zone will be obtained in the stellar envelope. This helps to form a longer blue loop. The extension of the blue loop is very sensitive to the parameters （Cx and αTCM） of the diffusive mixing model and of the TCM. The results mainly show that： 1） comparing the results of the classical model with the mixing-length theory, the lengths of the obtained blue loops with different combinations of the values of Cx and αTCM are all increased and the length of the blue loop increases with the values of parameters Cx and αTCM; 2） the diffusive mixing model can significantly extend the time of stellar models lingering on the blue side of the HR diagram, even though the length of the blue loop for the 7M⊙ star has a less prominent difference between the classical and diffusive mixing model; 3） both the observations referring to the location of the Cepheid instability strip and the number ratio NB/NR of blue to red evolved stars in the Galactic open clusters can confine the two parameters in a range of 0.5 ≤ αLTCM ≤ 0.9 and 10-5 ≤ Cx ≤ 10-4 for the model of 5M⊙. However, for the case of the 7M⊙ star, there seems to be no such definite range to even only account for the observed number ratio NB/NR. In any case, our results based on the diffusive mixing model are on the whole in accordance with not only other theoretical ones but also the observations.
文摘建立一种荧光染料叠氮溴化丙锭(PMA)与环介导等温扩增(LAMP)相结合的技术,同时利用羟基萘酚蓝(HNB)染料对婴儿配方奶粉中沙门氏菌活菌进行快速可视化检测。以沙门氏菌siiA基因为靶点,设计特异性引物,利用PMA抑制死菌DNA的扩增反应,反应前向体系中加入HNB染料,通过颜色变化对目标菌进行快速检测。结果表明,PMA的最佳处理浓度为3μg/mL,体系中HNB的最优浓度为150μmol/L,所构建的PMA-LAMP-HNB方法对沙门氏菌纯培养物的检测灵敏度为4.6×10^1CFU/mL,对人工污染婴儿配方奶粉中该菌的检测灵敏度为6.3×10^1CFU/g,是传统PCR方法的100倍,且总检测时间不超过1 h 30 min。
