Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) c...Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.展开更多
Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><spa...Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><span style="font-family:Verdana;">er-assisted selection into current breeding practices. In this study, we investigated, in </span><span style="font-family:Verdana;">White Fulani and Muturu cattle breeds, polymorphism of two candidate</span><span style="font-family:Verdana;"> genes (Smoothened—</span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> and Lipase Maturation Factor 1—</span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;">) that have been reported to influence body size in cattle. The </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene encodes for the SMO </span><span style="font-family:Verdana;">(smoothened) receptor protein;a membrane signal transducer that plays a</span><span style="font-family:Verdana;"> role in the control of bone formation and adipogenesis. </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1 </span><span style="font-family:Verdana;">encodes for the </span><span style="font-family:Verdana;">LMF1 protein which regulates post-translational maturation of vascular li</span><span style="font-family:Verdana;">pases. Desired regions of the genes were amplified and genotyped by direct </span><span style="font-family:Verdana;">DNA sequencing or Polymerase chain reaction—Restriction Fragment</span><span style="font-family:Verdana;"> Length </span><span style="font-family:Verdana;">Polymorphism. For association study, linear body measurements traits</span><span style="font-family:Verdana;"> (BMTs) that can be used to predict body size were measured on each animal and values of BMTs measured were compared between both breeds. Very significant (P < 0.001) differences, in favour of White Fulani, were observed for all the BMTs compared. Six (6) previously reported SNP (G21234C, C22424T and C22481T, T22939C, C23329T and T23458G) were found on the </span><i><span style="font-family:Verdana;">SMO</span></i><span> </span><span style="font-family:Verdana;">while </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;"> was monomorphic at the expected (T > C) exon 4 mutation site. Association analysis revealed that polymorphism on G21234C, C22424T, T22939C and T23458G loci significantly affected BMTs (P < 0.05 or 0.01) in both cattle breeds. The outcome of this study showed that </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene could be a useful </span><span style="font-family:Verdana;">candidate gene to facilitate marker assisted selection for body size while </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;"> is monomorphic in the cattle breeds.</span>展开更多
文摘Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.
文摘Cattle production is integral to the people of Africa and her economy. To <span style="font-family:Verdana;">improve cattle productivity, there is a need to inculcate molecular mark</span><span style="font-family:Verdana;">er-assisted selection into current breeding practices. In this study, we investigated, in </span><span style="font-family:Verdana;">White Fulani and Muturu cattle breeds, polymorphism of two candidate</span><span style="font-family:Verdana;"> genes (Smoothened—</span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> and Lipase Maturation Factor 1—</span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;">) that have been reported to influence body size in cattle. The </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene encodes for the SMO </span><span style="font-family:Verdana;">(smoothened) receptor protein;a membrane signal transducer that plays a</span><span style="font-family:Verdana;"> role in the control of bone formation and adipogenesis. </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1 </span><span style="font-family:Verdana;">encodes for the </span><span style="font-family:Verdana;">LMF1 protein which regulates post-translational maturation of vascular li</span><span style="font-family:Verdana;">pases. Desired regions of the genes were amplified and genotyped by direct </span><span style="font-family:Verdana;">DNA sequencing or Polymerase chain reaction—Restriction Fragment</span><span style="font-family:Verdana;"> Length </span><span style="font-family:Verdana;">Polymorphism. For association study, linear body measurements traits</span><span style="font-family:Verdana;"> (BMTs) that can be used to predict body size were measured on each animal and values of BMTs measured were compared between both breeds. Very significant (P < 0.001) differences, in favour of White Fulani, were observed for all the BMTs compared. Six (6) previously reported SNP (G21234C, C22424T and C22481T, T22939C, C23329T and T23458G) were found on the </span><i><span style="font-family:Verdana;">SMO</span></i><span> </span><span style="font-family:Verdana;">while </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;"> was monomorphic at the expected (T > C) exon 4 mutation site. Association analysis revealed that polymorphism on G21234C, C22424T, T22939C and T23458G loci significantly affected BMTs (P < 0.05 or 0.01) in both cattle breeds. The outcome of this study showed that </span><i><span style="font-family:Verdana;">SMO</span></i><span style="font-family:Verdana;"> gene could be a useful </span><span style="font-family:Verdana;">candidate gene to facilitate marker assisted selection for body size while </span><i><span style="font-family:Verdana;">LMF</span></i><span style="font-family:Verdana;">1</span><span style="font-family:Verdana;"> is monomorphic in the cattle breeds.</span>