An immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding bone marrow sections obtained from 10 patients with myelodysplastic syndrome (MDS) was presented. The results of qualitative...An immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding bone marrow sections obtained from 10 patients with myelodysplastic syndrome (MDS) was presented. The results of qualitative and quantitative immunohistochemical analysis are as follows: (1) Labile antigens of T lymphocytes were well preserved, thus allowing analysis of distribution of T lymphocyte subsets in situ ; (2) the average number of T 3, T 4 and T 8 lymphocyte of the diffuse infiltrate was about 2 %, 0.4 %, 0.5 %, respectively, of all nucleated cells in bone marrow, and T 4/T 8 of T cells were below 1.0 in patients with MDS; (3) there were cases of RAS showing T lymphocyte aggregation in bone marrow, but no patient exhibited progressive refractory anemia with excess of blasts(RAEB) and RAEB in transformation (RAEBT). These findings indicated that the immunological abnormalities are of importance in the evaluation of pathogenesis and prognosis of MDS.展开更多
目的:探讨骨髓活检采用乙二醇-甲基丙烯酸酯(glycol-methacrylate,GMA)塑胶包埋技术结合巨核细胞免疫标记方法在诊断以单纯血小板减少为表现的骨髓增生异常综合征(myelodysplastic syndrome,MDS)中的应用价值。方法:采用骨髓活检GMA塑...目的:探讨骨髓活检采用乙二醇-甲基丙烯酸酯(glycol-methacrylate,GMA)塑胶包埋技术结合巨核细胞免疫标记方法在诊断以单纯血小板减少为表现的骨髓增生异常综合征(myelodysplastic syndrome,MDS)中的应用价值。方法:采用骨髓活检GMA塑胶包埋及巨核细胞免疫标记技术,对47例表现为单纯血小板减少患者的骨髓标本进行病理学分析。结果:8例患者被诊断为MDS,其中3例为MDS伴单系病态造血(myelodysplastic syndrome with single lineage dysplasia,MDS-SLD),1例为5q-综合征,4例为MDS伴多系病态造血(myelodysplastic syndrome-multilineage dysplasia,MDS-MLD)。8例MDS患者的巨核细胞标志物均为阳性,其中6例的骨髓可见清晰的未成熟前体细胞异常定位(abnormal localization of immature precursors,ALIP)现象,且有3例患者检测到染色体异常。结论:采用骨髓活检塑胶包埋技术,细胞形态可清晰显示,有助于以单纯血小板减少为表现的MDS的诊断,有助于MDS的明确诊断及辅助分型;巨核细胞免疫标记有助于医师辨认小、微巨核细胞等特征性的巨核系病态造血表现,由此可以鉴别以单纯血小板减少为表现的MDS,故2种方法结合在诊断MDS方面具有应用价值。展开更多
文摘An immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding bone marrow sections obtained from 10 patients with myelodysplastic syndrome (MDS) was presented. The results of qualitative and quantitative immunohistochemical analysis are as follows: (1) Labile antigens of T lymphocytes were well preserved, thus allowing analysis of distribution of T lymphocyte subsets in situ ; (2) the average number of T 3, T 4 and T 8 lymphocyte of the diffuse infiltrate was about 2 %, 0.4 %, 0.5 %, respectively, of all nucleated cells in bone marrow, and T 4/T 8 of T cells were below 1.0 in patients with MDS; (3) there were cases of RAS showing T lymphocyte aggregation in bone marrow, but no patient exhibited progressive refractory anemia with excess of blasts(RAEB) and RAEB in transformation (RAEBT). These findings indicated that the immunological abnormalities are of importance in the evaluation of pathogenesis and prognosis of MDS.
文摘目的:探讨骨髓活检采用乙二醇-甲基丙烯酸酯(glycol-methacrylate,GMA)塑胶包埋技术结合巨核细胞免疫标记方法在诊断以单纯血小板减少为表现的骨髓增生异常综合征(myelodysplastic syndrome,MDS)中的应用价值。方法:采用骨髓活检GMA塑胶包埋及巨核细胞免疫标记技术,对47例表现为单纯血小板减少患者的骨髓标本进行病理学分析。结果:8例患者被诊断为MDS,其中3例为MDS伴单系病态造血(myelodysplastic syndrome with single lineage dysplasia,MDS-SLD),1例为5q-综合征,4例为MDS伴多系病态造血(myelodysplastic syndrome-multilineage dysplasia,MDS-MLD)。8例MDS患者的巨核细胞标志物均为阳性,其中6例的骨髓可见清晰的未成熟前体细胞异常定位(abnormal localization of immature precursors,ALIP)现象,且有3例患者检测到染色体异常。结论:采用骨髓活检塑胶包埋技术,细胞形态可清晰显示,有助于以单纯血小板减少为表现的MDS的诊断,有助于MDS的明确诊断及辅助分型;巨核细胞免疫标记有助于医师辨认小、微巨核细胞等特征性的巨核系病态造血表现,由此可以鉴别以单纯血小板减少为表现的MDS,故2种方法结合在诊断MDS方面具有应用价值。
