Objective To investigate the efficacy and mechanism of tibial transverse bone transfer in the treatment of lower limb ulcer wounds in diabetic rabbits.Methods Animal models of diabetic foot lower extremity ischemia we...Objective To investigate the efficacy and mechanism of tibial transverse bone transfer in the treatment of lower limb ulcer wounds in diabetic rabbits.Methods Animal models of diabetic foot lower extremity ischemia were made,and grouped Animal models of diabetic foot lower extremity ischemia were made,and grouped control studies were designed to evaluate the time of wound healing and the expression level of vascular endothelial growth factor(VEGF),platelet derived growth factor(PDGF),and epidermal cell growth factor(EGF)in the wound during the same period of time.Results In animal experiments,transverse tibial bone transfer can shorten the healing time of diabetic ulcer and increase the expression of wound growth factor(VEGF,PDGF,EGF).Conclusion In animal experiments,diabetes can inhibit the expression of growth factors(VEGF,PDGF,EGF)in ulcer wounds,and tibial transverse bone transfer can promote wound healing.The mechanism may be related to the increased expression of VEGF,PDGF and EGF in the wound surface.展开更多
We have shown previously that high-efficient gene transfer can be attained in primary hematopoietic cells using liposome-mediated gene transfer strategy. In order to examine the stability of gene expression mediated b...We have shown previously that high-efficient gene transfer can be attained in primary hematopoietic cells using liposome-mediated gene transfer strategy. In order to examine the stability of gene expression mediated by this gene transduction protocol, we observed the expression of marker gene in vivo by using bone marrow transplantation (BMT) to engraft lethally irradiated mouse with the genetically modified hematopoietic cells. The results showed that the mouse transplanted with appropriated number of transduced cells remained alive andhealthy. The PCR analysis and G418 selection of the spleen colonies and bone marrow cells isolated from lethally irradiated animals 15 days and 30 days after injection of genetically modified bone marrow cells showed that the progeny cells of the transduced hematopoietic stem cells still contained and expressed the transduced genes, suggesting that the hematopoietic system is at least partially re-constructed by the stem cells with marker gene and that the stable expression of foreign genes in vivo can be attained by using this easy and harmless transduction protocol. These findings provide experimental basis for clinician to further investigate the biology of marrow reconstruction and the mechanism of leukemia relapse after BMT.展开更多
Objective To develop retrovirus-mediated transfer of the fusion gene encoding EGFP-BMP_2 in mesenchymal stem cells.Methods Mesenchymal stem cells from New Zealand white rabbits were transduced with retroviral pLEGFP-B...Objective To develop retrovirus-mediated transfer of the fusion gene encoding EGFP-BMP_2 in mesenchymal stem cells.Methods Mesenchymal stem cells from New Zealand white rabbits were transduced with retroviral pLEGFP-BMP_2 vector by the optimized retroviral transduction protocol.Fluorescent microscopy's examination was to evaluate the results of the transduction,flow cytometer's analysis was to evaluate the transduction efficiency and the Fluorescence-activated cell sorting method was to sort the transduced cells.Bioactivity test from C_2C_12K_4 cells was to show the expression and bio-activity of the fusion gene.Results Fluorescent microscopy showed the success of the transduction.By flow cytometer's analysis,the mean efficiency of the transduction with EGFP was(42.8±6.1)% SD.Transduced cells were sorted efficiently by the fluorescence-activated cell sorting method and after sorting,almost of those showed the expression of BMP_2.Fluorescently and strongly bioactivity test for C_2C_12K_4 cells demonstrated that fluorescent materials were located the surface of cells and the activity of luciferase increased compared with the control.Analysis of long-term expression showed there was no difference between 2 week-time point and 3 month-time point of culture post-sorting.Conclusion Mesenchymal stem cells can be transduced efficiently by retrovirus-mediated transfer of the fusion gene encoding EGFP-BMP_2,the highly pure transduced cells are obtained by the fluorescence-activated cell sorting technique,the expressed chimeric protein embraced the double bioactivity of EGFP and BMP_2,and moreover,the expression had not attenuated over time.展开更多
目的:探讨氨基质子转移成像(amide proton transfer imaging,APT)定量参数对初诊前列腺癌患者骨转移风险的预测价值。方法:回顾并分析2019年7月—2022年3月于重庆大学附属肿瘤医院行APT检查的42例未经治疗的初诊前列腺癌患者的影像学及...目的:探讨氨基质子转移成像(amide proton transfer imaging,APT)定量参数对初诊前列腺癌患者骨转移风险的预测价值。方法:回顾并分析2019年7月—2022年3月于重庆大学附属肿瘤医院行APT检查的42例未经治疗的初诊前列腺癌患者的影像学及临床资料,患者均经前列腺穿刺活检或手术后病理学检查确诊,其中无骨转移组27例,骨转移组15例。采用独立样本t检验或秩和检验分析各参数在预测前列腺癌骨转移风险方面的差异。通过logistic回归模型测算参数联合应用的预测概率,并绘制受试者工作特征(receiver operating characteristic,ROC)曲线进行分析。结果:年龄、睾酮、黄体生成素、卵泡刺激素、泌乳素、雌二醇、前列腺影像报告和数据系统(Prostate Imaging Reporting And Data System,PIRADS)评分在两组之间的差异无统计学意义(P>0.05),而总前列腺特异性抗原(prostate-specific antigen,PSA)、APT平均值(APTw)、表观弥散系数(apparent diffusion coefficient,ADC)、前列腺病理学Gleason评分及血清碱性磷酸酶在预测前列腺癌骨转移风险方面差异有统计学意义(P<0.05),前列腺癌骨转移阳性组的APTw(2.989±1.018)高于阴性组(2.273±1.015)。ROC曲线分析结果显示,APTw独立预测前列腺癌骨转移风险的特异度较高(88.46),所有参数联合应用的预测能力最佳(曲线下面积为0.866)。结论:APT定量参数与前列腺癌骨转移密切相关,对初诊前列腺癌患者转移风险有较好的预测价值。展开更多
文摘Objective To investigate the efficacy and mechanism of tibial transverse bone transfer in the treatment of lower limb ulcer wounds in diabetic rabbits.Methods Animal models of diabetic foot lower extremity ischemia were made,and grouped Animal models of diabetic foot lower extremity ischemia were made,and grouped control studies were designed to evaluate the time of wound healing and the expression level of vascular endothelial growth factor(VEGF),platelet derived growth factor(PDGF),and epidermal cell growth factor(EGF)in the wound during the same period of time.Results In animal experiments,transverse tibial bone transfer can shorten the healing time of diabetic ulcer and increase the expression of wound growth factor(VEGF,PDGF,EGF).Conclusion In animal experiments,diabetes can inhibit the expression of growth factors(VEGF,PDGF,EGF)in ulcer wounds,and tibial transverse bone transfer can promote wound healing.The mechanism may be related to the increased expression of VEGF,PDGF and EGF in the wound surface.
文摘We have shown previously that high-efficient gene transfer can be attained in primary hematopoietic cells using liposome-mediated gene transfer strategy. In order to examine the stability of gene expression mediated by this gene transduction protocol, we observed the expression of marker gene in vivo by using bone marrow transplantation (BMT) to engraft lethally irradiated mouse with the genetically modified hematopoietic cells. The results showed that the mouse transplanted with appropriated number of transduced cells remained alive andhealthy. The PCR analysis and G418 selection of the spleen colonies and bone marrow cells isolated from lethally irradiated animals 15 days and 30 days after injection of genetically modified bone marrow cells showed that the progeny cells of the transduced hematopoietic stem cells still contained and expressed the transduced genes, suggesting that the hematopoietic system is at least partially re-constructed by the stem cells with marker gene and that the stable expression of foreign genes in vivo can be attained by using this easy and harmless transduction protocol. These findings provide experimental basis for clinician to further investigate the biology of marrow reconstruction and the mechanism of leukemia relapse after BMT.
基金This work was supported by the National Natural Science Foundation of China(No.30400163).
文摘Objective To develop retrovirus-mediated transfer of the fusion gene encoding EGFP-BMP_2 in mesenchymal stem cells.Methods Mesenchymal stem cells from New Zealand white rabbits were transduced with retroviral pLEGFP-BMP_2 vector by the optimized retroviral transduction protocol.Fluorescent microscopy's examination was to evaluate the results of the transduction,flow cytometer's analysis was to evaluate the transduction efficiency and the Fluorescence-activated cell sorting method was to sort the transduced cells.Bioactivity test from C_2C_12K_4 cells was to show the expression and bio-activity of the fusion gene.Results Fluorescent microscopy showed the success of the transduction.By flow cytometer's analysis,the mean efficiency of the transduction with EGFP was(42.8±6.1)% SD.Transduced cells were sorted efficiently by the fluorescence-activated cell sorting method and after sorting,almost of those showed the expression of BMP_2.Fluorescently and strongly bioactivity test for C_2C_12K_4 cells demonstrated that fluorescent materials were located the surface of cells and the activity of luciferase increased compared with the control.Analysis of long-term expression showed there was no difference between 2 week-time point and 3 month-time point of culture post-sorting.Conclusion Mesenchymal stem cells can be transduced efficiently by retrovirus-mediated transfer of the fusion gene encoding EGFP-BMP_2,the highly pure transduced cells are obtained by the fluorescence-activated cell sorting technique,the expressed chimeric protein embraced the double bioactivity of EGFP and BMP_2,and moreover,the expression had not attenuated over time.
文摘目的:探讨氨基质子转移成像(amide proton transfer imaging,APT)定量参数对初诊前列腺癌患者骨转移风险的预测价值。方法:回顾并分析2019年7月—2022年3月于重庆大学附属肿瘤医院行APT检查的42例未经治疗的初诊前列腺癌患者的影像学及临床资料,患者均经前列腺穿刺活检或手术后病理学检查确诊,其中无骨转移组27例,骨转移组15例。采用独立样本t检验或秩和检验分析各参数在预测前列腺癌骨转移风险方面的差异。通过logistic回归模型测算参数联合应用的预测概率,并绘制受试者工作特征(receiver operating characteristic,ROC)曲线进行分析。结果:年龄、睾酮、黄体生成素、卵泡刺激素、泌乳素、雌二醇、前列腺影像报告和数据系统(Prostate Imaging Reporting And Data System,PIRADS)评分在两组之间的差异无统计学意义(P>0.05),而总前列腺特异性抗原(prostate-specific antigen,PSA)、APT平均值(APTw)、表观弥散系数(apparent diffusion coefficient,ADC)、前列腺病理学Gleason评分及血清碱性磷酸酶在预测前列腺癌骨转移风险方面差异有统计学意义(P<0.05),前列腺癌骨转移阳性组的APTw(2.989±1.018)高于阴性组(2.273±1.015)。ROC曲线分析结果显示,APTw独立预测前列腺癌骨转移风险的特异度较高(88.46),所有参数联合应用的预测能力最佳(曲线下面积为0.866)。结论:APT定量参数与前列腺癌骨转移密切相关,对初诊前列腺癌患者转移风险有较好的预测价值。
