Analysis of sex pheromone production and field trapping of the Asian corn borer(Ostrinia furnacalis Guenée)in Xinjiang,China

Identifying the sex pheromone systems of local pest populations facilitates their management,especially for moth species that show significant geographic variation in sex pheromone communication.We investigated the pheromone production and behavioral responses of the Asian corn borer(Ostrinia furnacalis Guenée;ACB)in Xinjiang,China.The ACB produces three compounds:(Z)-12-tetradecenyl acetate(Z12-14:Ac)and(E)-12-tetradecenyl acetate(E12-14:Ac)which are two sex pheromone compounds,and n-tetradecyl acetate(14:Ac)which has variable roles in mediating behavioral responses.The ratios of these three compounds produced in female gland are geographically distinct among different populations.Quantitative analysis of pheromone production showed that the proportions of Z12-14:Ac in the E/Z isomers(i.e.,Z and E12-14:Ac)and the proportions of 14:Ac in the ternary blend respectively averaged 60.46%(SD=5.26)and 25.00%(SD=7.37),with their probabilities normally or near-normally distributed.Trapping experiments in a cornfield indicated that deploying the E/Z isomers and the three compounds in rubber septa close to their gland ratios yielded the most captured males,while other ratios that deviated from the gland ratios showed reduced field captures.The ternary blend was significantly more attractive to males than the E/Z isomers in the field,indicating a functional role of 14:Ac as the third pheromone component used by the local population.Additionally,the dose-response test demonstrated that the application of the three compounds at dosages between 200 and 350μg attracted significantly more males compared to other dosages.Therefore,the characterization of this local ACB pheromone system provides additional information about its geographic variation and serves as a basis for optimizing the pheromone-mediated control of this pest in Xinjiang.

TEMPERATURE DEPENDENT DEVELOPMENT OF ASIAN CORN BORER Ostrinia furnacalis

Temperature dependent development in the Asian corn borer, Ostrinia furnacalis (Guenee) was determined at nine constant temperatures between 10℃ and 34℃. Except for 10℃ development of all life stages occurred at the temperatures tested, however, mortality was significantly great at the extreme temperatures(12℃ and 34℃). Egg, larvae and pupae duration accounted for 17%, 57% and 25% of total one of immature stage, respectively Lower developmental thresholds estimated to be 10 38, 10 06 and 11 07℃ for eggs, larvae and pupae, respectively. Upper limited thresholds were 28 00, 31 00 and 31 00℃ for eggs, larvae and pupae, respectively. The heat unit requirements for egg stage were 79 15 degree days, for larval stage were 339 73 degree days, and for pupal stage were 128 82 degree days, respectively. Overall, heat unit requirements for development from egg to adult were 539 91 degree days between lower developmental threshold 10 35℃ and upper limited threshold 32℃.

Composition of Rice Stem Borers in Western Rice Areas of Sichuan Province and New Characteristics of Their Occurrence and Damage

[ Objective ] The paper was to study the composition of rice stem borers in western Sichuan Province and the characteristics of their occurrence and dam- age. [ Method ] Rice stem borers in western rice areas in Sichuan Province were composed of Chilo suppressalis, Tryporyza itwertulas and Sesamla inferens. C. sup- pressalis was the dominant population in most rice areas in western flee areas in Sichuan Province. T. incertulas had distribution and damage in Longquan, Qingbai- jiang and some rice areas, showing increasing trend of spread. The population of S. inferens was the minimum, but it caused serious damage in late period of rice. [ Conclusion] The result provided theoretical basis for proposing effective control measures against rice stem borers in western rice areas in Sichuan Province.

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

Immature embryos of rice varieties "Xiushui11" and "Chunjiang 11" precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

Progress on Sex Pheromone Synthesis of Asian Corn Borer, Ostrinia furnacalis (Guenee)

The progress on the components identification, synthesis methods （syn- thetic strategy of carbon chain increasing and synthetic strategy of carbon chain de- creasing） of Asian corn borer （Ostrinia fumacalis （Guenee）） sex pheromone was reviewed, which will provide a reference for the control of Asian corn borer.

Effects of Droplet Distribution on Insecticide Toxicity to Asian Corn Borers(Ostrinia furnaealis) and Spiders(Xysticus ephippiatus)

Distribution of horizontal boom produced droplets downwards into maize canopies at flowering period and its effects on the efficacies of emamectin benzoate, lambda-cyhalothrin and chlorantraniliprole against the second generation of Asian corn borer （ACB） larvae and their toxicity to spiders were studied. When insecticides were sprayed downwards into the maize canopies, randomly filtering out droplets by upper leaves led to great variations of droplet coverage and density within the canopies. Consequently, the efficacies of lambda-cyhalothrin and emamectin benzoate against ACB larvae were decreased because of randomly filtering out droplets by upper leaves. But field investigation showed that lambda-cyhalothrin was extremely toxic to hunting spiders, Xysticus ephippiatus, and not suitable to IPM programs in regulation of the second generation of ACB. Therefore, randomly filtering out droplets by upper leaves decreased lambda-cyhalothrin＇s efficacy against ACB larvae, but did little to decrease its toxicity to X. ephippiatus. Amamectin benzoate can reduce the populations of X. ephippiatus by 58.1-61.4%, but the populations can recover at the end of the experiment. Chlorantraniliprole was relatively safe to X. ephippiatus. It only reduced the populations of X. ephippiatus by 22.3-33.0%, and the populations can totally recover 9 d after application.

Do rice water weevils and rice stem borers compete when sharing a host plant?

The rice water weevil （RWW） Lissorhoptrus oryzophilus Kuschel （Coleoptera： Curculionidae） is an invasive insect pest office Oryza sativa L. in China. Little is known about the interactions of this weevil with indigenous herbivores. In the present study, adult feeding and population density of the weevil, injury level of striped stem borer Chilo suppressalis （Walker） （Lepidoptera： Pyralidae） and pink stem borer Sesamia inferens （Walker） （Lepidoptera： Noctuidae） to flee, as well as growth status of their host plants were surveyed in a rice field located in Southeastern Zhejiang, China, in 2004 with the objective to discover interspecific interactions on the flee. At tillefing stage, both adult feeding of the weevil and injury of the stem borers tended to occur on larger tillers （bearing 5 leaves） compared with small tillers （bearing 2-4 leaves）, but the insects showed no evident competition with each other. At booting stage, the stem borers caused more withering/dead hearts and the weevil reached a higher density on the plants which had more productive tillers and larger root system; the number of weevils per tiller correlated negatively with the percentage of withering/dead hearts of plants in a hill. These observations indicate that interspecific interactions exist between the rice water weevil and the rice stem borers with negative relations occurring at booting or earlier developmental stages of rice.

Virulence and mycotoxic effects of Metarhizium anisopliae on Mahogany shoot borer,Hypsipyla robusta(Lepidoptera:Pyralidae)

Developing appropriate control measures for the Mahogany shoot borer, Hypsipyla robusta Moore has become increasingly important due to the severe damaging effect of the pest on the establishment of the saplings of Swietenia mahagoni Jacq （Sapindales： Meliaceae）. Existing management methods are largely limited to silvicultural practices and spraying of chemical insecticides. To identify a potential fungal biocontrol agent, we compared the virulence of six native and two standard ARSEF isolates of Metarhizium anisopliae Metsch. against this pest. The average survival time and conidial yield of IWST-Ma7 was higher （6.2 to 7.3 days and 4.9 to 4.7 x 105 conidia/ml） than the standards. Sig- nificant difference in sporulation on the cadavers between isolates, doses and incubation periods were substantiated for the selection of potential strain. The mycotoxic effects of crude soluble protein extract when in- corporated in the artificial diet, the ARSEF 2596 and ARSEF 3603 showed LDs0 value of 3.7% and 5.6%. However, IWST-Ma7 was highly lethal with significant lowest LDs0 value of 2.6%. The enzyme activity of IWST-Ma7 was highest for chitinase, CDA, protease and lipase viz., 1.90 U/mg, 1.80 U/mg, 0.98 U/mg and 0.80 U/mg respectively. However the enzyme activity of chitinase and Chitin deacetylase assay for all the isolates was significantly higher than protease and lipase activity. The ITS regions （5.8S rDNA and 28S rDNA） of seven isolates of M. anisopliae were amplified using the ITS1 and ITS4 primers which was a unique fragment of approximately 550 bp. Based on ITS regions, phy- logenetic tree have been constructed and the isolates have been grouped in to 5 clades. The virulence and mycotoxic effects of different isolates could rationally be used to employ them for the management of the mahogany borer.

Migratory flight of insect pests within a year-round distribution: European corn borer as a case study

Insect migratory flight differs fundamentally from most other kinds of flight behavior, in that it is non-appetitive. The adult is not searching for anything, and migratory flight is not terminated by encounters with potential resources. Many insect pests of agricultural crops are long-distance migrants, moving from lower latitudes where they overwinter to higher latitudes in the spring to exploit superabundant, but seasonally ephemeral, host crops. The migratory nature of these pests is somewhat easy to recognize because of their sudden appearance in areas where they had been absent only a day or two earlier. Many other serious pests survive hostile winter conditions by diapausing, and therefore do not require migration to move between overwintering and breeding ranges. Yet there is evidence of migratory behavior engaged in by several pest species that inhabit high latitudes year-round. In these cases, the consequences of migratory flight are not immediately noticeable at the population level, because migration takes place for the most part within their larger year-round distribution. Nevertheless, the potential population-level consequences can be quite important in the contexts of pest management and insect resistance management. As a case study, I review the evidence for migratory flight behavior by individual European corn borer adults, and discuss the importance of understanding it. The kind of migratory behavior posited for pest species inhabiting a permanent distribution may be more common than we realize.

Relationship between Resistance of Rice Stem Borer and Insecticide Use Intensity in South China

[Objective] This study aimed to survey the effect of insecticide use intensity on the resistance of rice stem borer in South China. [Method] The data includes 19 counties from Guangdong Province and Guangxi Province from 1991 to 2010. A fixed effect model was established to evaluate the effect of insecticide use intensity on the resistance of rice stem borer. [Result] The regression results suggest that one-period lagged total number of insecticide sprays has significant effect on the infestation level of rice stem borer. [Conclusion] Although rice stem borer is a migratory rice pest, insecticide use intensity has significant effect on the resistance level of rice stem borer in South China.

Differential effects of insecticides on mitochondrial membrane fluidity and ATPase activity between the wolf spider and the rice stem borer

Differential effects of methamidophos and three pyrethroids on ATPase activity and membrane fluidity of mitochondria were investigated between the wolf spider（Pirata subpiraticus（Boes.et Str.））and the rice stem borer（Chilo suppressalis（Walker））.Based on a comparison of LD_（50） values,the toxicities of the tested insecticides were higher to the wolf spider than to the rice stem borer.Cyhalothrin at 1×10^（–4） mmol L^（–1） caused inhibition of the mitochondrial Na~＋-K~＋-ATPase and Ca^（2＋）-Mg^（2＋）-ATPase activities,and it’s inhibitions on Na＋-K＋-ATPase and Ca^（2＋）-Mg^（2＋）-ATPase activities were significantly higher in the wolf spider（44 and 28%）than in the rice stem borer（19 and 11%）.Methamidophos at 1×10^（–4） mmol L^（–1） decreased Ca^（2＋）-Mg^（2＋）-ATPase activity by 16 and 27%in the wolf spider and the rice stem borer,respectively,but no significant effect on the specific activity of Na＋-K＋-ATPase was observed.The DPH（1,6-diphenyl-1,3,5-hexatriene）fluorescence polarization values of mitochondrial membranes were not significantly affected by methamidophos in either species.However,cyhalothrin and alpha-cypermethrin induced the values of DPH polarization of mitochondrial membrane increasing with the concentration of cyhalothrin and alpha-cypermethrin from 20 to 100μmol L^（–1） in the rice stem borer and the wolf spider.Effect of ethofenprox on fluidity of the wolf spider and the rice stem borer was contrary.These results suggest that both inhibition of membrane ATPase and changes of membrane fluidity could be appended to the action mechanisms of pyrethroid insecticides.

Transgenic japonica rice expressing the cry1C gene is resistant to striped stem borers in Northeast China

Rice production and quality are seriously affected by the lepidopteran pest,striped stem borer(SSB),in Northeast China.In this study,a synthetic cry1 C gene encoding Bacillus thuringiensis(Bt)δ-endotoxin,which is toxic to lepidopteran pest,was transformed into a japonica rice variety(Jigeng 88)in Northeast China by Agrobacterium-mediated transformation.Through molecular detection and the Basta resistance germination assay,a total of 16 single-copy homozygous transgenic lines were obtained from 126 independent transformants expressing cry1 C.Finally,four cry1 C-transgenic lines(JL16,JL23,JL41,and JL42)were selected by evaluation of the Cry1 C protein level,insect-resistance and agronomic traits.The cry1 C-transgenic lines had higher resistance to SSB and higher yield compared with non-transgenic(NT)control plants.T-DNA flanking sequence analysis of the transgenic line JL42 showed that the cry1 C gene was inserted into the intergenic region of chromosome 11,indicating that its insertion may not interfere with the genes near insertion site.In summary,this study developed four cry1 C-transgenic japonica rice lines with high insect resistance and high yield.They can be used as insect-resistant germplasm materials to overcome the problem of rice yield reduction caused by SSB and reduce the use of pesticides in Northeast China.

Invasive sequence and ecological niche of main insect borers of Larix gmelinii forest in Aershan, Inner Mongolia

After the outbreak of Dendrolimus superans Buter in 2002, many insect borers quickly invaded larch （Larix gmelinii Rupr.） forests in the Aershan of Inner Mongolia. Methods involved included setting sample plots, collecting adults in iron traps and measuring areas of galleries to study the invasive sequence, their ecological niche and the extent of the different effects by the main insect borers to their hosts. The results showed that the damage of D. superans weakened L. gmelinii, first Ips subelongatus Motschulsky invaded, followed by Acanthocinus carinulatus Gebler, Monochamus urussovi Fisher and M. sutor L. After the outbreak of D. superans, the average density of longhorn beetles per L. gmelinii tree increased. The ecological niche of Ips subelongatus stretches almost from the base to the top of the trunk. The number of insects in older stands of L. gmelinii is larger than those in middle aged stands. They do not damage healthy trees of L. gmelinii. The ecological niche of A. carinulatus is higher in dead L. gmelinii trees than in weak ones. The degree of damage is directly proportional with age and depth of bark. M. urussovi mainly damages trunks below 4 m in weak trees; in dead trees they can do damage up to 6 m in height. M. sutor mainly damages trunks below 5 m in weak L. gmelinii trees; in dead trees they cause damage up to 7 m. Again, the degree of damage is directly proportional with age. None of the three species of longhorn beetles damage healthy L. gmelinii and younger trees. Among the main insect borers, the degree of damage caused by I. subelongatus is more serious than that of other insects.

Field Efficacy Test of 22% Fipronil FS against Sugarcane Stem Borers and Thrips

[ Objective] The paper was to explore the field efficacy of 22% fipronil FS against sugarcane stem borer and thrips （Baliothrips serratus Kobus ）. [ Method] The field trial was conducted by foliar spraying of 22% fipronil FS and mixed broadcasting with fertilizer in 2014. [ Result ] Foliar spraying of 22% fipronil FS at the dose of 50 mIV667 m~ had the best control effect against sugarcane stem borer, and the control effect at 90 d post administration was 70.70%, significantly higher than that of control agent （61.86%）. Medium and high dose of 22% fipronil FS had better control effects against sugarcane thrips. [ Conclusion ] Considering control effects against sugarcane stem borers and thrips as well as sugarcane yield, it is recommended that 22% fipronil FS is sprayed at medium concentration （50 mL/667 m2 ）.

Isolation and pathogenicity of fungi associated to ambrosia borer (<i>Euplatypus segnis</i>) found injuring pecan (<i>Carya illinoensis</i>) wood

Euplatypus segnis is an insect pest of economic importance in pecan (Carya illinoensis) trees grown at Parras, General Cepeda and Torreón Coahuila, Mexico. The objectives in this study-were to identify the fungal strains associated to ambrosia borer body and diseased pecan wood and determine their pathogenicity. The results showed that the associated fungi to Euplatypus segnis and damaging the pecan wood were identified as: Helminthosporium sp., Aspergillus sp., Penicillium sp., Phoma sp., Ascochyta sp., Phaecylomices sp., Umbeliopsis sp., Torula sp., Fusarium solani, Alternaria alternata, Fusarum oxysporum, and Lasiodiplodia theobromae. The pathogenicity tests on healthy 3 year old pecan trees cv. western using Fusarium oxysporum, Fusarium solani, Alternaria alternata and Lasiodiplodia theobromae suspension conidia shown die back tree branches after 84 days inoculation. The insect in combination with the fungal invasion eventually cause the death of trees. Additionally, the insect contributes to the spread of fungi in pecan nut orchards.

Effect of Intercropping Pattern on Stem Borer Infestation in Pearl Millet (<em>Pennisetum glaucum</em>L.) Grown in the Nigerian Sudan Savannah

Fields experiments were conducted at the Department of Crop Protection, Faculty of Agriculture, University of Maiduguri Teaching and Research Farm, Maiduguri during 2010 and 2011 cropping seasons to investigate the effects of intercropping pattern on stem borer pest infestation in pearl millet (Pennisetum glaucum L.) intercropped with ground nut (Arachis hypogea L.). A split-plot design was used to test the intercrop pattern of 1:0 ratio (sole millet), 1:1 ratio (1 millet row to 1 ground nut row), 2:1 ratio (2 millet rows to 1 ground nut row) and 1:2 ratio (1 millet row to 2 ground nut rows). The results obtained showed that the intercrop pattern of 1:2 ratio and 1:1 ratio yielded less stem borer infestation and abundance in pearl millet, and as well supported high panicle weight and grain yield. In addition to recommending either of these two intercropping patterns to pearl millet farmers for more effective stem borer pest management, results further show the need for identification of effective intercropping patterns in other cropping systems.

The insecticide resistance in stripped stem borer,Chilo suppressalis(Walker)

The stripped stem borer (SSB),Chilo suppressalis (Walker) is one ofthe major insect pests of rice in Chi-na. Chemical control has been acommon practice in SSB managementsince 1950s. Insecticides used in-cluded BHC before 1983; organophos-

Comparative Efficacies of Next-Generation Insecticides Against Yellow Stem Borer and Their Effects on Natural Enemies in Rice Ecosystem

The efficacies of some next-generation insecticides against the rice yellow stem borer(YSB),Scirpophaga incertulas(Walk.), and their compatibilities with natural enemies were investigated during 2014 and 2015. Three newer insecticides, chlorantraniliprole 0.4% G, dinotefuran 20% SG, and methoxyfenozide 24% SC, and two commonly used insecticides, carbufuran 5 G and quinalphos 25 EC,were evaluated in the field for their efficacies against YSB and their non-target effects on natural enemies.Application of chlorantraniliprole 0.4% G at 10.96 kg/hm^2 resulted in the greatest reduction in YSB infestation(deadhearts and whiteheads) and greatest increase of yield compared to the untreated control plots, followed by methoxyfenozide 24% SC at 0.41 L/hm^2, dinotefuran 20% SG at 0.15 kg/hm^2,carbufuran 5 G at 10.96 kg/hm^2, and quinalphos 25 EC at 1.50 L/hm^2. All the insecticides reduced the numbers of predators viz., lady bird beetles, wolf spiders, carabid beetles, earwigs, green mirid bugs, and damselflies. Numbers of adults of the egg parasitoids Trichogramma sp., Telenomus sp. and Tetrastichus sp. were significantly reduced in insecticide-treated plots compared to untreated control plots. In all field trials, the harmful effects of the five insecticides were in the following rank order(least harmful to most harmful): chlorantraniliprole 0.4% G, carbufuran 5 G, dinotefuran 20% SG, methoxyfenozide 24% SC,and quinalphos 25 EC. On the basis of reduction in YSB infestation, increase in grain yield, and compatibility with natural enemies, chlorantraniliprole 0.4% G was proved to be the best of all the insecticides for YSB management system, although the study suggested minimizing its retail price for enhancement of cost effectiveness in farmers' fields.

Identification of Bt-transgenic Rice Plants for Resistance to Stripe Stem Borer (Chilo suppressalis) and Genetic Analysis of the Transgenes

Resistance to herbicide Basta was used to identify Bt-transgenic rice plants and the progenies of crosses between the Bt-transgenic rice and the rice varieties cultivated in the Huang Huai area of China. The results demonstrated that the Basta-positive rice plants were highly resistant to stripe stem borer (Chilo sup-pressalis) both in the laboratory and field tests. Both cryIA (b) and bar genes were expressed and co-inherited in both self ing and crossing progenies. Mendelian segregation of the marker gene bar was observed in F2 and BC1 progenies. The results implicates that it is possible to transfer cryIA (b) gene into other cultivated varieties through crosses and back crosses.

Integration and Application of Comprehensive Prevention and Control Technology for Sugarcane Borers in Low Latitude Plateau of China

Aimed at the difficult problem of prevention and control of disastrous sugarcane borers in low latitude plateau of China,this research systematically overcame the bottleneck of comprehensive prevention and control of sugarcane bores after 10 years of collaborative research,and formed a number of core product technologies,which promoted the green and high-quality development of the sugarcane industry and achieved the following innovative results.In this study,the species,population structure and damage characteristics of disastrous sugarcane borers in low latitude plateau were clarified for the first time,which provided a scientific basis for the formulation of technology and application of comprehensive prevention and control.The monitoring technology system and early warning monitoring network were constructed for the monitoring of borer population in low latitude plateau sugarcane area,which realized the accurate monitoring of the population dynamics of sugarcane bores and provided technical support for comprehensive prevention and control.The flight spraying technology by unmanned aerial vehicle(UAV)was developed for bores in the low latitude plateau cane area,and it has been successfully applied in a large area,thereby opening up a new way of light,simple and efficient for effective control of borers.The scale application of lamp trapping and sexual attracting to kill adult insects technology promoted the advancement of green control technology of bores.Integration was made to the comprehensive control techniques like UAV flight control,biological agents,physical and chemical inducement and control,and sexual attractant trapping in the low latitude plateau sugarcane area,and standardized technical regulations have been formulated,which significantly improved the overall prevention and control effect of large areas.The technologies for the comprehensive prevention and control of sugarcane borers have been popularized and applied in 8 major sugarcane producing states(cities)in low altitude plateau of Yunnan over the years,which has controlled the damage and showed remarkable control effect.In 2017-2018,the integrated control technology was popularized and applied to a total of 2.78×10^5 hm^2(1.33×10^4 hm^2 of UAV flight control)of area,which recovered a total of 3.666×10^6 t of sugarcane losses,increasing 4.69×10^5 t of sugar with the sales increase of 3.979 billion yuan,added profits of 1.251 billion yuan and the taxes increase of 188 million yuan.The research results show that the technology has high technical integration,strong practicability,high degree of transformation,significant economic,social and ecological benefits,which provide technical supports for the sustainable development,loss reduction and efficiency increase of the sugarcane industry in the low-latitude plateau sugarcane region,and make significant contributions to the economic development of ethnic minorities in the border-stricken areas and the increase of farmers income and poverty alleviation.