To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, th...To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.展开更多
We examined the effect of linseed supplementation in finishing diet on intramuscular fat level and fatty acid composition of three bovine muscles （longissimus dorsi-LD, semitendinosus-ST, psoas major-PM）, total of s...We examined the effect of linseed supplementation in finishing diet on intramuscular fat level and fatty acid composition of three bovine muscles （longissimus dorsi-LD, semitendinosus-ST, psoas major-PM）, total of sixty-two young bulls, from six different genotypes Angus （A）, Charolais （CH）, Holstein-Friesian （H）, Hungarian Grey （HG）, Hungarian Simmental （HS）, Charolais x Hungarian Grey F1 （CHxHG）. The results showed that A bulls had the highest intramuscular fat （IM） level （3.76）, particularly in PM （4.74）, followed by HG （3.40）, crossbred HG （3.05）, CH （2.33）, HS （2.21）, with the lowest level for H （2.11） bulls. IMF for LD （2.87） and ST （1.77） muscles were significantly lower than PM （3.60） for all groups. SFA was significantly higher for PM than the other two, due to the high level oflM. The highest MUFA was detected in LD, significantly different from others. ST contained the highest PUFA （12.99）, followed by LD （10.30） and PM （10.19）. The n-6 fatty acids were affected either by breed, or by muscle type. The linoleic acid was the highest in muscle samples of Holstein bulls except for PM, while the lowest was in A bulls in all cases. The same tendency can be seen in all cases for long chain n-6 fatty acids. The overall mean of n-3 fatty acids varied among genotypes from 1.36 to 1.80%. The highest level was measured in ST of CH and H （2.19）, whereas the lowest one in LD of HG （1.22）. Significant differences among genotypes were shown only for PM. The level of n-3 series long chain fatty acids was the highest for ST, for LD and PM in H, CH and HS, respectively. The ratio of n-6/n-3 ranged from 4.48 to 6.22 and was significantly less favorable in H than in A bulls. The highest CLA level was detected in all cases in HG bulls.展开更多
基金Supported by the Ministry of Agricultural Nuarture of New Varieties Genetically Modified Organisms Significant Special Funding (2008ZX08007-002)
文摘To examine the effect of myogenin gene on the differentiation of bovine skeletal muscle satellite cell, we constructed small interfering RNA plasmid vector to obtain myogenin knockdown bovine skeletal muscle cells, then used cell transfection, real time RCR and Western Blot to detect the influence of myogenin to cell differentiation. Results showed that the knockdown of myogenin significantly decreased its expression and other muscle-specific genes. Compared to the control, it could differentiate into few myotubes when challenged by low serum in the medium. These findings provided an important theoretical basis for further explore of the genetic mechanism in adult skeletal muscle, the remedy of muscle injuries and the cultivation of high-yield transgenic cattle.
文摘We examined the effect of linseed supplementation in finishing diet on intramuscular fat level and fatty acid composition of three bovine muscles （longissimus dorsi-LD, semitendinosus-ST, psoas major-PM）, total of sixty-two young bulls, from six different genotypes Angus （A）, Charolais （CH）, Holstein-Friesian （H）, Hungarian Grey （HG）, Hungarian Simmental （HS）, Charolais x Hungarian Grey F1 （CHxHG）. The results showed that A bulls had the highest intramuscular fat （IM） level （3.76）, particularly in PM （4.74）, followed by HG （3.40）, crossbred HG （3.05）, CH （2.33）, HS （2.21）, with the lowest level for H （2.11） bulls. IMF for LD （2.87） and ST （1.77） muscles were significantly lower than PM （3.60） for all groups. SFA was significantly higher for PM than the other two, due to the high level oflM. The highest MUFA was detected in LD, significantly different from others. ST contained the highest PUFA （12.99）, followed by LD （10.30） and PM （10.19）. The n-6 fatty acids were affected either by breed, or by muscle type. The linoleic acid was the highest in muscle samples of Holstein bulls except for PM, while the lowest was in A bulls in all cases. The same tendency can be seen in all cases for long chain n-6 fatty acids. The overall mean of n-3 fatty acids varied among genotypes from 1.36 to 1.80%. The highest level was measured in ST of CH and H （2.19）, whereas the lowest one in LD of HG （1.22）. Significant differences among genotypes were shown only for PM. The level of n-3 series long chain fatty acids was the highest for ST, for LD and PM in H, CH and HS, respectively. The ratio of n-6/n-3 ranged from 4.48 to 6.22 and was significantly less favorable in H than in A bulls. The highest CLA level was detected in all cases in HG bulls.
