Experimental Study on Stability of Breakwaters with Penetrating Box Foundations

The breakwater with top sealed, shallow and wide penetrating box foundations is a new type of structure, applicable to deep water and soft seabed. The relations of horizontal and vertical bearing capacities of the box foundation structure as well as the instability induced failure modes to its dimensions and external loads are discussed through static model tests and wave tests. The mechanical properties of the stability of the box foundation are similar to those of embedded rigid foundations, i.e. the vertical stresses at the bottom of the box are distributed in a linear pattern under the action of vertical loads, and passive and active soil pressures are developed at the front and back sides of the box under the action of horizontal loads; there are two instability induced failure modes of the foundation structure-horizontal slide along the box base and tilting due to insufficient local vertical bearing capacity of the soil beneath the box base. The stability of box foundations can be analyzed by use of the methods applied to analysis of the embedded rigid foundations. To increase the width of the box is the most effective way to improve the stability of box foundations.

Centrifugal model test and numerical simulation of vertical earth pressure on soft foundation box culvert

To obtain the vertical earth pressure on a soft foundation box culvert and investigate the interaction of the soil-culvert-foundation system, both a centrifugal model test and a numerical simulation were conducted and the comparisons with the current methods to determine the load on a culvert were completed. The results of the model test and numerical analysis are in satisfactory agreement, which shows that the direction of the shear stress between the culvert and the adjacent embankment depends on the differential settlement between them. A vertical earth pressure concentration appears on the culvert with a rigid piles foundation because of a downward shear stress. The ratio of the load on a soft foundation culvert and the overburden pressure above the culvert raises first and then decreases as the backfill height increases. In order to reduce the load on a culvert, it is suggested to limit the stiffness difference of the foundations under the culvert and embankment and to use a light backfill over the culvert.

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.

Box-Behnken响应面优化超声联合双酶法提取当归地上部分总黄酮及其抗氧化活性研究

试验以当归废弃物(当归地上部分)为试验材料,在单因素试验基础上,设计Box-Behnken响应面试验,优化超声联合双酶法提取当归地上部分总黄酮的最佳提取工艺,采用DPPH和ABTS自由基法和FRAP法评价当归地上部分总黄酮的抗氧化活性。结果显示,利用超声联合双酶法提取当归地上部分总黄酮的最佳条件为乙醇浓度50%、液料比50∶1、超声时间41 min、双酶(纤维素酶∶果胶酶=2∶1)用量0.68 g/L、酶解时间93 min,此条件下,当归地上部分总黄酮提取率为8.18%。当归地上部分具有较高的抗氧化活性,在浓度为150 mg/L时,对DPPH自由基清除率为90.3%,略低于VC。在浓度为3 000 mg/L时,对ABTS清除率为88.6%,ABTS的IC50为DPPH IC50近30倍,FRAP抗氧化能力为4.64 (mg VC eq/g sample)。试验表明,当归地上部分的总黄酮具有抗氧化活性。

NS-box系统在地下基坑支护中的适用性研究

以某地铁基坑为例,采用极限平衡法和有限元数值模拟对NS-box系统在基坑地下连续墙支护结构中的应用进行了计算和分析,并从技术经济性、施工速度、施工复杂程度、适用范围以及环境影响等方面与传统的地下连续墙支护结构进行对比研究。研究结果表明,NS-box系统应用于地下连续墙结构是安全、可行的,其在施工工艺和环境保护等方面具有明显优势,可应用于基坑地下连续墙支护结构。

Y-box结合蛋白-1与肿瘤发生

Y-box结合蛋白(Y-box binding protein,YB)是一类广泛存在于多种生物中的蛋白质家族,是一类高度保守的顺式作用调控元件,也是一种广泛表达的转录因子,发挥重要的生物学功能。Y-box结合蛋白-1(Y-box binding protein-1,YB-1)是Y-box结合蛋白家族成员之一,参与基因的转录调节、翻译调控、维持mRNA的稳定性、mRNA选择性剪接、RNA的配对、DNA的修复、细胞增殖和再生等生物学功能。大量研究表明YB-1的异常表达与许多肿瘤,如:前列腺癌、乳腺癌、肺癌、宫颈癌、淋巴瘤、卵巢癌的发生发展具有相关性。对YB-1的研究为肿瘤的治疗提供了新的方向,并可能成为肿瘤诊治的一种新的标志物。

Box-Behnken设计毛细管液相色谱法拆分α-氨基酸对映体的研究

将L-脯氨酸通过3-缩水甘油丙基三甲氧基硅烷键合到球型多孔硅胶基质上,制备了手性配体交换色谱固定相。采用高压匀浆法将制备的填料填充到毛细管柱中,在自组装的毛细管液相色谱仪上对3种α-氨基酸对映体进行了拆分。利用Box-Behnken设计对影响因素(流动相中乙腈的体积分数、中心离子Cu2+的浓度、流速)进行优化,以最大保留时间和分离度为响应值,借助二次多元回归方程,建立了相应的数学模型。响应曲面图表明:DL-天冬酰胺在5%乙腈、5 mmol/L Cu2+、流速0.139μL/min,苏氨酸在10%乙腈、5mmol/L Cu2+、流速0.167μL/min,丝氨酸在5%乙腈、7 mmol/L Cu2+、流速0.167μL/min的条件下均能得到基线分离。表明通过所制备的手性固定相,采用配体交换色谱模式可获得拆分DL-氨基酸的理想效果。

Box-Behnken响应面设计法优化排脓散水提工艺

目的优选排脓散最佳水提工艺。方法采用Box-Behnken响应面设计法,以水提液中3个指标性成分(柚皮苷、新橙皮苷、芍药苷)的含量和出膏率的综合评价作为考察指标,考察料液比、浸泡时间、提取时间3个因素对提取工艺的影响。结果排脓散水提的最佳工艺条件为15倍水,浸泡67min,提取74min。结论 Box-Behnken实验设计法优化排脓散的水提工艺方法可行,预测性较好。

An analytic study on the deflection of subway tunnel due to adjacent excavation of foundation pit

Predicting and estimating the response of sub- way tunnel to adjacent excavation of foundation pit is a research focus in the field of underground engineering. Based on the principle of two-stage method and incre- mental method, an analytic approach is suggested in this paper to solve this problem in an accurate and rapid way, and the upheavals of tunnel due to adjacent excavation are solved by analytic method. Besides, the presented method is used in the practical engineering case of Shenzhen Metro Line 11 and verified by numerical simulation and in situ measurement. Finally, a parametric analysis is performed to investigate the influence of different factors on tunnel＇s deflection. Some useful conclusions have been drawn from the research as below： The deflection results of tunnel obtained from analytic method are nearly consistent with the results getting from numerical analysis and measured data, which verified the accuracy and rationality of pre- sented method. The excavation size has a significant impact on both the displacement values and influenced range of tunnel. However, the relative distance only impacts the displacement values of tunnel, but not the influenced range of tunnel. It may provide certain reference to analyze the deflection of subway tunnel influenced by adjacent excavation.

Powell dynamic identification of displacement parameters of indeterminate thin-walled curve box based on FCSE theory

The FCSE controlling equation of pinned thinwalled curve box was derived and the indeterminate problem of continuous thin-walled curve box with diaphragm was solved based on flexibility theory. With Bayesian statistical theory,dynamic Bayesian error function of displacement parameters of indeterminate curve box was founded. The corresponding formulas of dynamic Bayesian expectation and variance were deduced. Combined with one-dimensional Fibonacci automatic search scheme of optimal step size,the Powell optimization theory was utilized to research the stochastic identification of displacement parameters of indeterminate thin-walled curve box. Then the identification steps were presented in detail and the corresponding calculation procedure was compiled. Through some classic examples,it is obtained that stochastic performances of systematic parameters and systematic responses are simultaneously deliberated in dynamic Bayesian error function. The one-dimensional optimization problem of the optimal step size is solved by adopting Fibonacci search method. And the Powell identification of displacement parameters of indeterminate thin-walled curve box has satisfied numerical stability and convergence,which demonstrates that the presented method and the compiled procedure are correct and reliable.During parameters鈥？iterative processes,the Powell theory is irrelevant with the calculation of finite curve strip element（FCSE） partial differentiation,which proves high computation effciency of the studied method.

MiR-183-5p promotes the progression of non-small cell lung cancer through targeted regulation of FOXO1

Objective To investigate miR-183-5p targeting to forkhead box protein O1(FOXO1)and its corresponding effect on the proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT)of non-small cell lung cancer(NSCLC)cells.Methods NSCLC tissues and adjacent normal tissues from 60 patients with NSCLC adenocarcinoma were obtained via pathological biopsy or intraoperative resection.Several cell lines were cultured in vitro,including the human normal lung epithelial cell line BEAS-2B and human NSCLC cell lines A549,SPCA-1,PC-9,and 95-D.miR-183-5p and FOXO1 mRNA expression in tissues and cells were detected by qRT-PCR;the corresponding correlations in NSCLC tissues were analyzed using the Pearson test,and the relationship between miR-183-5p expression and clinicopathological parameters was analyzed.The miR-183-5p-mediated regulation of FOXO1 was verified by bioinformatics prediction alongside double luciferase,RNA-binding protein immunoprecipitation(RIP)assay,and pull-down experiments.A549 cells were divided into control,anti-miR-NC,anti-miR-183-5p,miR-NC,miR-183-5p,miR-183-5p+pcDNA3.1,and miR-183-5p+pcDNA3.1-FOXO1 groups.Cell proliferation,invasion,migration,apoptosis,and cell cycle distribution were detected using an MTT assay,clone formation assay,Transwell assay,scratch test,and flow cytometry,respectively.The expression of EMT-related proteins in the cells was analyzed by western blotting.The effect of miR-185-3p silencing on the development of transplanted tumors was detected by analyzing tumor formation in nude mice.Results miR-183-5p expression was significantly higher in NSCLC tissues and cells than in adjacent normal tissues,whereas FOXO1 mRNA expression was significantly down-regulated.There was a significant negative correlation between miR-183-5p and FOXO1 mRNA in NSCLC tissues(P<0.05).Additionally,the expression of miR-183-5p was significantly correlated with tumor size,tumor differentiation,and tumor-node-metastasis stage in patients with NSCLC(P<0.05).miR-183-5p targeted and inhibited FOXO1 expression.Compared to the anti-miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the anti-miR-183-5p group,whereas the protein expression of E-cadherin andα-catenin and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion were significantly lower in the anti-miR-183-5p group(P<0.05).Compared to the miR-NC group,the cell proliferation,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells in the miR-183-5p group were significantly higher,whereas the E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly lower;furthermore,the frequency of colony formation and invasion were significantly higher in the miR-183-5p group(P<0.05).Compared with the miR-183-5p+pcDNA3.1 group,the OD value,scratch healing rate,N-cadherin and vimentin protein expression,and the proportion of S phase cells were significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group,whereas E-cadherin andα-catenin protein expression and the proportion of G0/G1 phase cells were significantly higher;additionally,the frequency of colony formation and invasion was significantly lower in the miR-183-5p+pcDNA3.1-FOXO1 group(P<0.05).Overall,silencing miR-185-3p inhibited the growth of transplanted tumors and promoted FOXO1 expression.Conclusion Overexpression of miR-183-5p can inhibit apoptosis and promote the proliferation,migration,invasion,and EMT,of NSCLC cells by down-regulating FOXO1 expression.

隧道侧穿箱基框架结构体系振动台试验及数值模拟

以郑州市轨道交通7号线侧穿箱基框架结构工程为背景,开展了室内振动台缩尺模型试验,并借助有限元软件ABAQUS对不同工况进行数值建模,将振动台试验和数值模拟结果进行对比,验证数值模拟结果的可靠性。基于验证后的模型,分析隧道侧穿箱基框架结构体系的动力响应特性。结果表明:①隧道或箱基框架结构的存在均会降低整个体系的自振频率;②箱基框架结构的存在会降低隧道结构的加速度响应,同时减弱隧道结构所受到的弯矩,而对其所受剪力的影响不大;③隧道结构的存在会减弱箱基框架结构的加速度响应和位移响应。箱室的存在会改变地震波的传播路径,且当箱基框架结构高于三层时,楼板加速度响应随楼层高度的增大更明显。

大倾角钢箱提篮拱桥总体设计

考虑施工技术、经济性和景观效果等方面,陕西蔡家坡渭河特大桥主桥采用(35+210+35) m大倾角钢箱提篮拱桥。该桥采用摩擦摆和环向钢丝绳阻尼支座综合减隔震约束体系,以降低结构的地震响应。拱肋为单室等宽钢箱截面,采用主、副拱肋2条悬链线,主、副拱肋共面,设计为横向内倾20°的大倾角,在提高桥梁景观效果的同时,又有效提高了结构的整体稳定性;拱肋设钢-混结合段连接拱脚与混凝土拱座。桥面系采用自重较轻的正交异性钢桥面板结构。吊杆采用抗拉强度1 860 MPa的环氧柔性钢绞线。基础采用Φ2.5 m的钻孔灌注群桩,以抵抗软弱地质条件下荷载的竖向及水平作用力。该桥总体采用先梁后拱的施工方案。整体及局部有限元分析结果表明,桥梁结构力学性能均满足规范要求,设计安全可靠。

Box－Behnken效应面法优化石杉碱甲纳米结构脂质载体处方

目的采用Box-Behnken效应面法筛选石杉碱甲纳米结构脂质载体最佳处方。方法采用熔融超声-高压匀质法制备石杉碱甲纳米结构脂质载体,分别以混合脂质(X1)、混合乳化剂(X2)和脂药比(X3)为考察对象,以粒径(Y1)、包封率(Y2)和载药量(Y3)为评价指标,利用三因素三水平Box-Behnken效应面设计法筛选石杉碱甲纳米结构脂质载体的最佳处方。结果按最优处方制备的纳米粒粒径为(121.67±3.21)nm、包封率为(89.18±0.28)%、载药量为(1.46±0.05)%,与预测值偏差均小于5%。结论采用Box-Behnken效应面法优化石杉碱甲纳米结构脂质载体处方是有效、可行的。

均匀土-箱基-结构相互作用体系的计算分析

采用通用有限元程序ANSYS ,针对振动台试验中的均匀土 -箱基 -结构试验进行了三维有限元分析 ,计算中土体的本构模型采用等效线性模型 ,利用面 -面接触单元考虑土体与基础交界面的状态非线性。计算表明 ,基础底面和土体发生了滑移 ,基础侧面和土体之间发生了滑移和脱离 ;上部结构柱顶加速度反应主要由基础转动引起的摆动分量组成。通过与试验结果的对照研究 ,二者得出的规律基本一致 ,验证了采用的计算模型与分析方法的合理性 ,为进一步计算研究和实际工程应用奠定了基础。

考虑土-结构相互作用的高层建筑抗震分析

本文采用通用有限元程序ANSYS,针对上海地区一例土-箱基-高层建筑结构进行了三维有限元分析,计算中土体的本构模型采用等效线性模型,利用粘-弹性人工边界作为土体的侧向边界,并研究了土体边界位置、土性、基础埋深、基础形式以及上部结构刚度等参数对动力相互作用体系动力特性及地震反应的影响。

土-箱基-框架结构动力相互作用体系试验研究

通过对1/2大比例模型的试验与仿真分析,研究了结构-箱基础-土体系的动力反应.将实测基频结果与基于刚性地基的仿真分析结果对比,发现实测结果小于有限元结果;但将实测基频结果与考虑相互作用的仿真分析结果对比,发现实测结果与仿真分析结果吻合,这说明土-箱基础-结构相互作用改变了结构体系的动力特性,使结构体系的自振周期延长,同时也验证了仿真分析的有效性.

高层建筑下桩-箱基础共同作用研究

为了研究在上海软土地区上高层建筑桩－箱基础共同作用机理，本文对一高层建筑桩－箱基础进行了原位测试及共同作用分析。原位测试从基坑开挖开始到建筑物结构封顶后３个月，历时２年零３个月，取得了有关建筑物沉降特征、基底反力分布和桩顶作用力分布规律的实测资料。文中还按弹性理论方法对建筑物的群桩基础作了共同作用分析。

基于ARM和IEC 61131-3标准的软逻辑控制系统

介绍了基于S3C44B0X和IEC61131-3标准的软逻辑控制系统原理;给出了在uClinux系统下如何实现IEC61131-3标准的软逻辑控制的方法;提出了一种I/O映射机制,解决了编程系统定义的I/O变量和实际物理I/O口的关联问题。

新福菌素对人鼻咽癌细胞株CNE-2凋亡杀伤作用的研究

目的探讨新福菌素对人鼻咽癌细胞株CNE-2的体外杀伤效应及作用机制。方法体外培养CNE-2细胞,应用MTT比色法观察新福菌素对CNE-2细胞增殖的抑制作用。倒置显微镜下观察实验组细胞的生长及结构变化,同时采用逆转录-聚合酶链反应技术(RT-PCR)检测Bax mRNA基因的表达。结果新福菌素在体外对CNE-2细胞有较强的增殖抑制作用,能诱导CNE-2细胞凋亡;同时进行Bax mRNA基因表达检测发现对照组CNE-2细胞Bax mRNA表达呈弱阳性;实验组CNE-2细胞Bax mRNA表达均呈阳性,且随着新福菌素浓度的增加而逐渐增强。结论新福菌素对CNE-2细胞有明显的杀伤效应,杀伤机制可能是诱导鼻咽癌细胞凋亡,并与凋亡相关基因Bax mRNA表达上调有关。