Immune cell signatures and causal association with irritable bowel syndrome:A mendelian randomization study

BACKGROUND The mucosal barrier's immune-brain interactions,pivotal for neural development and function,are increasingly recognized for their potential causal and therapeutic relevance to irritable bowel syndrome(IBS).Prior studies linking immune inflammation with IBS have been inconsistent.To further elucidate this relationship,we conducted a Mendelian randomization(MR)analysis of 731 immune cell markers to dissect the influence of various immune phenotypes on IBS.Our goal was to deepen our understanding of the disrupted brain-gut axis in IBS and to identify novel therapeutic targets.AIM To leverage publicly available data to perform MR analysis on 731 immune cell markers and explore their impact on IBS.We aimed to uncover immunophenotypic associations with IBS that could inform future drug development and therapeutic strategies.METHODS We performed a comprehensive two-sample MR analysis to evaluate the causal relationship between immune cell markers and IBS.By utilizing genetic data from public databases,we examined the causal associations between 731 immune cell markers,encompassing median fluorescence intensity,relative cell abundance,absolute cell count,and morphological parameters,with IBS susceptibility.Sensitivity analyses were conducted to validate our findings and address potential heterogeneity and pleiotropy.RESULTS Bidirectional false discovery rate correction indicated no significant influence of IBS on immunophenotypes.However,our analysis revealed a causal impact of IBS on 30 out of 731 immune phenotypes(P<0.05).Nine immune phenotypes demonstrated a protective effect against IBS[inverse variance weighting(IVW)<0.05,odd ratio(OR)<1],while 21 others were associated with an increased risk of IBS onset(IVW≥0.05,OR≥1).CONCLUSION Our findings underscore a substantial genetic correlation between immune cell phenotypes and IBS,providing valuable insights into the pathophysiology of the condition.These results pave the way for the development of more precise biomarkers and targeted therapies for IBS.Furthermore,this research enriches our comprehension of immune cell roles in IBS pathogenesis,offering a foundation for more effective,personalized treatment approaches.These advancements hold promise for improving IBS patient quality of life and reducing the disease burden on individuals and their families.

Diet and physical activity influence the composition of gut microbiota,benefit on Alzheimer's disease

Alzheimer's disease is a neurodegenerative disease with complex etiology.Gut microbiota influences the gutbrain axis,which may affect pathways related to the pathogenesis of Alzheimer's disease.Additionally,diet and physical activity are likely to affect the pathology of Alzheimer's disease as well as the gut microbiota.This demonstrates that it may be possible to prevent or halt the progression of Alzheimer's disease by regulating the gut microbiota using diet and physical activity strategies.Therefore,the present study reviews the association between these two interventions and gut microbiota in the human body.It also summarizes how these two interventions benefit Alzheimer's disease.Furthermore,the primary limitations of these two interventions are discussed and promising strategies are proposed,which may be beneficial to further study and develop the intervening measure for the progression of Alzheimer's disease.

Effect of amitriptyline on gastrointestinal function and brain-gut peptides: A double-blind trial

AIM: To study the effects of low-dose amitriptyline (AMT) on gastrointestinal function and brain-gut peptides in healthy Chinese volunteers. METHODS: This was a double-blind, randomised, placebo-controlled, two-period cross-over trial. Twentyeight healthy volunteers were randomised and administered 1-wk treatments of AMT (12.5 mg tid) or placebo. Before and during the final two days of treatment, gastric emptying, proximal gastric accommodation and visceral sensitivity were measured by drinkingultrasonography test; the orocecal transit time (OCTT) was measured by lactulose hydrogen breath test, and fasting blood was collected. Plasma levels of ghrelin, motilin and neuropeptide Y (NPY) were measured by enzyme-linked immunosorbent assay kits.RESULTS: AMT slowed the OCTT (109.2 ± 29.68 min vs 96.61 ± 23.9 min, P = 0.004) but did not affect liquid gastric emptying and had no effect on proximal gastric accommodation. AMT resulted in decreases in the visual analogue scale (VAS) for difficulty in drinking 600 and 800 mL of water (3.57 ± 0.94 vs 2.98 ± 0.85, 5.57 ± 0.82 vs 4.57 ± 0.98, P < 0.01 for both), although it had no significant effect on the VAS for difficulty in drinking 200 mL and 400 mL of water. AMT significantly increased the plasma ghrelin level (442.87 ± 176.79 pg/mL vs 526.87 ± 158.44 pg/mL, P = 0.04) and the neuropeptide-Y level (890.15 ± 131.46 pg/mL vs 965.64 ± 165.63 pg/mL, P = 0.03), whereas it had no effect on the MTL level. CONCLUSION: Low-dose AMT could slow OCTT, make the stomach less sensitive and increase the plasma levels of ghrelin and NPY. Thus, we recommend the use of low-dose AMT for functional gastrointestinal disorders.

Brain-gut axis in the pathogenesis of Helicobacter pylori infection

Helicobacter pylori (H. pylori) infection is the main pathogenic factor for upper digestive tract organic diseases. In addition to direct cytotoxic and proinflammatory effects, H. pylori infection may also induce abnormalities indirectly by affecting the brain-gut axis, similar to other microorganisms present in the alimentary tract. The brain-gut axis integrates the central, peripheral, enteric and autonomic nervous systems, as well as the endocrine and immunological systems, with gastrointestinal functions and environmental stimuli, including gastric and intestinal microbiota. The bidirectional relationship between H. pylori infection and the brain-gut axis influences both the contagion process and the host&#x02019;s neuroendocrine-immunological reaction to it, resulting in alterations in cognitive functions, food intake and appetite, immunological response, and modification of symptom sensitivity thresholds. Furthermore, disturbances in the upper and lower digestive tract permeability, motility and secretion can occur, mainly as a form of irritable bowel syndrome. Many of these abnormalities disappear following H. pylori eradication. H. pylori may have direct neurotoxic effects that lead to alteration of the brain-gut axis through the activation of neurogenic inflammatory processes, or by microelement deficiency secondary to functional and morphological changes in the digestive tract. In digestive tissue, H. pylori can alter signaling in the brain-gut axis by mast cells, the main brain-gut axis effector, as H. pylori infection is associated with decreased mast cell infiltration in the digestive tract. Nevertheless, unequivocal data concerning the direct and immediate effect of H. pylori infection on the brain-gut axis are still lacking. Therefore, further studies evaluating the clinical importance of these host-bacteria interactions will improve our understanding of H. pylori infection pathophysiology and suggest new therapeutic approaches.

New technologies to investigate the brain-gut axis

Functional gastrointestinal disorders are commonly encountered in clinical practice, and pain is their commonest presenting symptom. In addition, patients with these disorders often demonstrate a heightened sensitivity to experimental visceral stimulation, termed visceral pain hypersensitivity that is likely to be important in their pathophysiology. Knowledge of how the brain processes sensory information from visceral structures is still in its infancy. However, our understanding has been propelled by technological imaging advances such as functional Magnetic Resonance Imaging, Positron Emission Tomography, Magnetoencephalography, and Electroencephalography （EEG）. Numerous human studies have non-invasively demonstrated the complexity involved in functional pain processing, and highlighted a number of subcortical and cortical regions involved. This review will focus on the neurophysiological pathways （primary afferents, spinal and supraspinal transmission）, brainimaging techniques and the influence of endogenous and psychological processes in healthy controls and patients suffering from functional gastrointestinal disorders. Special attention will be paid to the newer EEG source analysis techniques. Understanding the phenotypic differences that determine an individual＇s response to injurious stimuli could be the key to understanding why some patients develop pain and hyperalgesia in response to inflammation/injury while others do not. For future studies, an integrated approach is required incorporating an individual＇s psychological, autonomic, neuroendocrine, neurophysiological, and genetic profile to define phenotypic traits that may be at greater risk of developing sensitised states in response to gut inflammation or injury.

Neuroimaging the brain-gut axis in patients with irritable bowel syndrome

AIM:To summarize and synthesize current literature on neuroimaging the brain-gut axis in patients with irritable bowel syndrome(IBS).METHODS:A database search for relevant literature was conducted using Pub Med,Scopus and Embase in February 2015.Date filters were applied from the year2009 and onward,and studies were limited to those written in the English language and those performed upon human subjects.The initial search yielded 797articles,out of which 38 were pulled for full text review and 27 were included for study analysis.Investigations were reviewed to determine study design,methodology and results,and data points were placed in tabular format to facilitate analysis of study findings across disparate investigations.RESULTS:Analysis of study data resulted in the abstraction of four key themes:Neurohormonal differences,anatomic measurements of brain structure and connectivity,differences in functional responsiveness of the brain during rectal distention,and confounding/correlating patient factors.Studies in this review noted alterations of glutamate in the left hippocampus(HIPP),commonalities across IBS subjects in terms of brain oscillation patterns,cortical thickness/gray matter volume differences,and neuroanatomical regions withincreased activation in patients with IBS:Anterio cingulate cortex,mid cingulate cortex,amygdala anterior insula,posterior insula and prefrontal cortex.A striking finding among interventions was the substantia influence that patient variables(e.g.,sex,psychologica and disease related factors)had upon the identification of neuroanatomical differences in structure and con nectivity.CONCLUSION:The field of neuroimaging can provide insight into underlying physiological differences that distinguish patients with IBS from a healthy population.

Role of the brain-gut axis in gastrointestinal cancer

Several studies have largely focused on the significant role of the nervous and immune systems in the process of tumorigenesis, including tumor growth, proliferation, apoptosis, and metastasis. The brain-gut-axis is a new paradigm in neuroscience, which describes the biochemical signaling between the gastrointestinal (GI) tract and the central nervous system. This axis may play a critical role in the tumorigenesis and development of GI cancers. Mechanistically, the bidirectional signal transmission of the brain-gut-axis is complex and remains to be elucidated. In this article, we review the current findings concerning the relationship between the brain-gut axis and GI cancer cells, focusing on the significant role of the brain-gut axis in the processes of tumor proliferation, invasion, apoptosis, autophagy, and metastasis. It appears that the brain might modulate GI cancer by two pathways: the anatomical nerve pathway and the neuroendocrine route. The simulation and inactivation of the central nervous, sympathetic, and parasympathetic nervous systems, or changes in the innervation of the GI tract might contribute to a higher incidence of GI cancers. In addition, neurotransmitters and neurotrophic factors can produce stimulatory or inhibitory effects in the progression of GI cancers. Insights into these mechanisms may lead to the discovery of potential prognostic and therapeutic targets.

Study on the regulation of brain-gut peptide by Shenling Baizhu San in functional diarrhea rats

Objective:To explore the therapeutic mechanism of Shenling Baizhu San (SLBZS) on functional diarrhea (FDr) by studying the brain-gut axis and related neuropeptides.Methods:Sixty male Wistar rats were randomly divided into the control group,model group,SLBZS-treated group and Montmorillonite Powder-treated group (MP-treated group) (n =15/group).Rats received gavage after the establishment of functional diarrhea.An equal volume of SLBZS solution and Montmorillonite Powder (MP) solution was administered to the SLBZS-treated group and MP-treated group,respectively,and an equal volume of distilled water was administered to the control group and the model group.The chemical components and targets related to SLBZS were identified from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and Traditional Chinese Medicine Integrated Database (TCMID).The effective chemical components were screened based on oral bioavailability (OB) and drug like-index (DL),and their biological functions were analyzed by GlueGO.Based on this screening,the expression of Cholecystokinin (CCK) and Ghrelin in the hypothalamus of rats was detected by real-time PCR (RT-PCR) and western blotting.Results:In this study,72 effective components and 190 core targets of SLBZS were screened.SLBZS may regulate smooth muscle contraction,energy metabolism and other biological processes.The results of RT-PCR showed that in the model group,the expression of CCK mRNA (P =.001) and Ghrelin mRNA (P =.000) increased significantly.Compared with the model group,CCK mRNA (P =.007) and Ghrelin mRNA (P =.001) levels in SLBZS-treated rats were decreased significantly.The results of western blotting showed that in the model group,the protein expression of CCK (P =.001) and Ghrelin (P =.000) increased significantly.The protein levels of CCK (P =.001) and Ghrelin (P =.005) in the SLBZS-treated group were decreased significantly compared with the model group.Conclusion:SLBZS improved functional diarrhea by regulating the brain-gut axis.Changes in the expressions of brain-gut peptide,CCK and Ghrelin might explain the pathogenesis of functional diarrhea related to brain-gut peptide and gastrointestinal hormone.

Application progress of Chinese medicine in the treatment of irritable bowel syndrome based on the brain-gut axis theory

Irritable bowel syndrome is one of the most common functional gastrointestinal diseases,with a global prevalence of about 12%[1].Modern studies have shown that the abnormality of brain-gut peptides is closely related to the occurrence of irritable bowel syndrome.This article starts with vasoactive intestinal peptide,substance P,serotonin,neuropeptide Y,corticotropin releasing factor,calcitonin gene-related peptide,cholecystokinin and other brain-gut peptides and their correlation with intestinal flora,to summarize the treatment of irritable bowel syndrome with traditional Chinese medicine in recent years.

Therapeutic effect of zhuodu theory on ulcerative colitis and its effect on brain-gut peptide in serum and inflammatory factor

Objective:To investigate the therapeutic effect of traditional Chinese medicine xiezhuojiedu decoction on ulcerative colitis(UC)based on zhuodu theory and its effect on serum brain-gut peptide and inflammatory factors.Methods:110 cases of UC patients were divided into 2 groups according to the random number table method,with 55 cases in each group.The control group was treated with mesalazine,and the treatment group was given oral administration of Chinese medicine xiezhuojiedu decoction on the basis of the control group.Both groups received continuous treatment for 8 weeks.Integral of TCM syndromes,serum inflammatory factor and brain-gut peptide before and after treatment were compared between the two groups.The clinical efficiency,mucosal healing rate and endoscopic response rate of the two groups were compared.Results:After treatment,Integral of the main TCM syndromes abdominal pain,diarrhea,abdominal distention,mucous hematochezia,tenesmus of the treatment group were lower than the control group(P<0.01),the levels of serum(Tumor necrosis factor-α,(TNF-α),Interleukin(IL)-33,Substance P(SP)were lower than the control group(P<0.01),the levels of IL-10,vasoactive intestinal peptide(VIP),Somatostatin(SS)were higher than the control group(P<0.01),the clinical efficiency and mucosal healing rate were higher than control group(P<0.05),The difference was statistically significant.Conclusion:Based on the zhuodu theory,the xiezhuojiedu decoction can effectively regulate the levels of inflammatory factor and brain-gut peptide in the treatment of UC,improve the symptoms of patients,and promote the repair of intestinal mucosa.It's effective in treatment.

Clinical Application and Mechanism of Acupuncture in the Brain-Gut Interaction

Acupuncture and moxibustion is an important part of traditional medicine.Acupuncture and moxibustion can affect the digestive function of human body by interfering with the interaction between the brain and the intestines,while simultaneously having a benign adjustment effect on the mental state.In recent years,a large number of clinical studies at home and abroad have further proved the importance of acupuncture and moxibustion in the treatment of physical and mental diseases,and related mechanism studies have provided modern scientific evidence for the intervention of acupuncture and moxibustion in the brain-gut interaction from the perspectives of intestinal flora and brain functional imaging.On this basis,we integrate relevant viewpoints to provide ideas and methods for future research.

Effect of Xiaoyao San on the brain-gut axis in rats after chronic immobilization stress

Objective:To investigate the effect of Xiaoyao San on the brain-gut axis in rats exposed to chronic immobilization stress (ClS).Methods:Rats were divided into control,model,and treatment groups.The rats belonging to the model and treatment groups were subjected to CIS for 21 consecutive days,during which they were administered Xiaoyao San decoction [3.854 g/(kg· d)] or vehicle by gavage,and their body weight gain,food intake and water intake were monitored.The rats were subsequently subjected to the open field test (OFT) and D-XyloSe absorption test,and the expression levels of neuropeptides secreted by the hypothalamus and stomach were determined by enzyme-linked immunosorbent assay (ELISA),radioimmune analysis,or real-time fluorescence quantitative polymerase chain reaction.Gastric mucosal morphology was also assessed.Results:The model rats exhibited complex brain-gut axis abnormalities following exposure to CIS,abnormalities signified by decreases in food intake,reductions in digestive absorption,decreases in body weight,decreases in the total distances traveled and increases in the time in the central zone during the OFT,gastric mucosal lesion development and decreases in gastrointestinal hormone secretion.These changes were reversed after treatment with Xiaoyao San,which also regulated the secretion of both peripheral (serum and stomach) and central (hypothalamus) brain-gut peptides.Specifically,the levels of neuropeptide Y (NPY) and neuropeptide Y receptor Y5,which are secreted by the hypothalamus and promote digestive function,were increased in the Xiaoyao San-treated group compared with the model group.Furthermore,the levels of pro-opiomelanocortin (POMC) and its receptor,melanocortin-4 receptor (MC4R),which are secreted by the hypothalamus and inhibit digestive function,were significantly decreased in the treatment group compared with the model group.However,the levels of ghrelin (GHRL),gastrin (GAS) and motilin (MTL),which are secreted by the stomach,were significantly increased in the serum and stomach of the treatment group compared with the serum and stomach of the model group following Xiaoyao San treatment (P <.05 vs.the model group).Conclusion:Xiaoyao San attenuates CIS-induced gastrointestinal dysregulation by regulating the peptides secreted by both the hypothalamus and the gastrointestinal tract (GIT),suggesting that its effects are associated with the brain-gut axis.

基于脑—肠互动理论探讨从肝肺论治肠易激综合征

肠易激综合征(IBS)是临床常见且容易复发的消化系统疾病之一,西医发病机制尚不完全明确,但近年来研究发现脑—肠互动学说在IBS发病机制中有重要作用。中医病机与气机升降失调、津液输布失常密不可分,病位集中在肝、肺、大肠,肝肺与大肠之间的互动联系与现代医学中的脑肠互动学说异曲同工。此文通过分析脑肠互动学说解释从肝肺论治IBS的可行性,为中医临床辨治IBS提供理论支持。

Mediating effect of acarbose on neurotransmitters in mental disorders: a review

Acarbose is used to control postpran-dial blood glucose in patients with type 2 diabetes and impaired glucose tolerance,since it improves insulin resistance and reduces blood lipids and cardiovascular complications.However,in recent years,many studies have found that acarbose can mediate and regulate a variety of neurotransmitter-related diseases,although the mechanisms are not clear.Therefore,this paper analyzes the clinical effect of acarbose and its mediating effect on neurotransmitters of mental disorders through insulin,braingut axis,and calorie restriction,to provide a reference for the new clinical applications of acarbose.

薏苡仁生物肽对高糖损伤人脐静脉内皮细胞保护作用

目的:研究薏苡仁生物肽对高糖损伤人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)的保护作用及机制。方法:采用30 mmol/L葡萄糖建立HUVEC高糖损伤模型,以不同浓度薏苡仁生物肽作用于高糖损伤HUVEC后,利用硝酸还原酶法检测细胞NO分泌量;流式细胞仪检测细胞活性氧(reactive oxygen species,ROS)含量;Annexin V-FITC/PI双染检测细胞凋亡情况;实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qPCR)检测炎症相关基因表达量。结果:与正常对照组相比,高糖损伤导致HUVEC NO分泌量显著降低28.72%(P<0.05),ROS分泌量增加96.19%(P<0.05),ICAM-1、IL-6、MCP-1和VCAM基因表达量明显增加;随着高糖作用时间延长,凋亡细胞比例显著增加,作用14 d后细胞凋亡率为正常对照组的1.61倍。不同浓度薏苡仁生物肽均能够不同程度地增加NO分泌量,降低ROS含量和炎症基因表达量,减少细胞凋亡。结论:薏苡仁生物肽对高糖损伤HUVEC具有一定的保护作用。

中国对虾主要过敏原的鉴定及理化性质

以中国对虾为研究对象,旨在获得有关中国对虾过敏原的基础数据,为水产品中过敏原的控制提供依据。首先利用聚丙烯酰胺凝胶电泳对中国对虾过敏原组分进行分析,经免疫印迹鉴定其主要过敏原。然后利用高效液相色谱法纯化中国对虾的主要过敏原,并用β消去、紫外扫描、肽质量指纹图谱和红外光谱等方法对其理化性质进行研究。表明中国对虾主要过敏原为分子量36ku的糖蛋白,糖含量为4.4%,存在O-型糖肽键,其二级结构主要为α-螺旋,与其他甲壳类过敏原之间存在很高的同源性。

玉米蛋白水解条件的优化研究

本试验是以玉米黄粉为底物,采用碱性蛋白酶为水解酶进行水解。采用二次旋转回归设计,以温度、酶与底物浓度比和pH三因素进行水解,得出玉米黄粉的最佳水解条件是[S]=8%、[E]/[S]=3.3%、pH =9.85、T=45℃、水解时间(t)=4.5h。在这种水解条件下蛋白回收率>73%。

临床指标对急性肾损伤合并心肾综合征患者行连续性肾脏替代治疗的预测价值

目的探讨多项临床指标对急性肾损伤(AKI)合并心肾综合征患者行连续性肾脏替代治疗(CRRT)的预测价值。方法选取青岛市市立医院2010年10月—2012年12月收治的50例合并心肾综合征的AKI住院患者为研究对象。保守治疗3 d后心功能好转者入选对照组(n=24),心功能无好转者继续行CRRT(CRRT组,n=26)。记录两组患者保守治疗前平均动脉压(MAP)、清蛋白(Alb)、血红蛋白(Hb)、尿素氮-Ⅰ、血肌酐-Ⅰ、B型钠尿肽-Ⅰ(BNP-Ⅰ)及第1个24 h尿量(24 h尿量-Ⅰ)和呋塞米用量(呋塞米-Ⅰ);记录保守治疗3 d后尿素氮-Ⅱ、血肌酐-Ⅱ、BNP-Ⅱ及入院第3个24 h尿量(24 h尿量-Ⅱ)、呋塞米用量(呋塞米-Ⅱ)。比较两组上述临床指标间的差异。采用ROC曲线下面积(AUC)评价各指标预测患者是否行CRRT的敏感度和特异度。结果对照组与CRRT组Alb、尿素氮-Ⅱ、血肌酐-Ⅱ、BNP-Ⅰ、BNP-Ⅱ、24 h尿量-Ⅰ、24 h尿量-Ⅱ比较,差异均有统计学意义(P<0.05);ROC曲线显示,BNP-Ⅱ、24 h尿量-Ⅰ与24 h尿量-Ⅱ的AUC均>0.700,具有较好的预测价值;BNP-Ⅱ的ROC曲线显示,取884 ng/L为切点时,其敏感度与特异度分别为92.3%与75.0%;24 h尿量-Ⅰ的ROC曲线显示,取2 395 ml/24 h为切点时,其敏感度与特异度分别为84.6%与75.0%;24 h尿量-Ⅱ的ROC曲线显示,取2 490 ml/24 h为切点时,其敏感度与特异度分别为96.2%与75.0%。结论经短期保守治疗后的24 h尿量与BNP水平可以较好地预测伴心肾综合征的AKI患者是否应该行CRRT。

辛伐他汀对慢性心力衰竭患者左室收缩功能及血浆脑钠肽水平变化的研究

目的观察辛伐他汀对慢性心力衰竭(CHF)患者左室收缩功能及血浆脑钠肽(BNP)的变化。方法经超声心动图检查,测出64例慢性心力衰竭患者以及20例健康对照者的左室舒张末内径(LVEDd)、左室射血分数(LVEF)及左室短轴缩短率(LVFS),应用酶联免疫吸附法(ELISA)测定两组脑钠肽(BNP)的浓度;将心衰患者随即分为30例常规治疗组和34例加用辛伐他汀组,1个月和6个月时再测定上述指标。结果心衰患者在治疗1个月LVEDd、LVEF及LVFS与治疗前比较均无统计学差异(P>0.05),6个月时的上述指标与治疗前比较差异有显著性(P<0.01),辛伐他汀组LVEF较常规治疗组LVEF升高更明显(P<0.05)。心衰患者在治疗前血浆BNP水平均高于正常对照组,治疗1个月和6个月血浆BNP水平均明显下降,但辛伐他汀组与常规治疗组同期比较下降更明显(P<0.05)。两组患者血浆BNP水平与LVEF呈负相关(P<0.01)。结论长期应用辛伐他汀可以改善慢性心力衰竭患者心功能和降低血浆BNP水平;慢性心力衰竭患者血浆BNP水平的改变早于心脏结构的变化,血浆BNP水平可作为治疗慢性心力衰竭的一个灵敏观察指标。

电针不同穴位对功能性便秘大鼠血清和组织中GAS、VIP的影响

目的观察不同穴位处方电针对功能性便秘大鼠血清和组织中GAS、VIP含量的影响,探讨电针治疗功能性便秘的机理。方法将SD大鼠50只随机分为5组,即正常组、模型组、电针Ⅰ组(取双侧天枢、大肠俞)、电针Ⅱ组(取双侧曲池、上巨虚)、电针Ⅲ组(取单侧天枢、大肠俞、曲池、上巨虚),模型组以冰水灌胃建立大鼠模型,电针各组分别按穴位给予电针治疗,每组连续治疗10天后检测血清和组织中GAS、VIP的变化。结果电针Ⅱ组血清和组织中GAS含量明显升高,VIP含量明显下降,与模型组比较有极显著性差异(P<0.01)。结论电针可能是通过提高功能性便秘大鼠血清中和组织中GAS含量和降低VIP含量而改善功能性便秘;电针Ⅱ组作用好于其他两组,说明取曲池、上巨虚治疗功能性便秘效果更好。