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Effects of Breviscapine on the Changes in Antioxidant Enzyme Activity Induced by Cerebral Ischemia-reperfusion in Rats 被引量:5
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作者 陈小夏 何冰 《Journal of Chinese Pharmaceutical Sciences》 CAS 1998年第2期35-37,共3页
\ Effects of breviscapine, the active ingredient isolated from Erigeron breviscapus (Vant) Handmazz, on the changes in antioxidant enzyme activity induced by cerebral ischemiareperfusion in rats were explored. It wa... \ Effects of breviscapine, the active ingredient isolated from Erigeron breviscapus (Vant) Handmazz, on the changes in antioxidant enzyme activity induced by cerebral ischemiareperfusion in rats were explored. It was found that breviscapine improved the activities of superoxide dismutase (SOD), GSHperoxidase and catalase, while decreasing the malondialdehyde (MDA) content in the brain, which was benificial in reducing the damage from cerebral ischemiareperfusion. 展开更多
关键词 \ breviscapine Cerebral ischemiareperfusion Superoxide dismutase GSHperoxidase CATALASE MALONDIALDEHYDE
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Mechanisms of Inhibitory Effects of Breviscapine on Lipid Peroxidation in Rat Brain Mitochondria 被引量:1
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作者 陈小夏 何冰 陈一岳 《Journal of Chinese Pharmaceutical Sciences》 CAS 1998年第4期42-46,共5页
The mechanisms by which breviscapine (Bre) inhibits the lipid preoxidation in rat brain mitochondria were investigated. The mitochondrial lipid peroxidation of rat brain induced by oxygen free radical was measured by ... The mechanisms by which breviscapine (Bre) inhibits the lipid preoxidation in rat brain mitochondria were investigated. The mitochondrial lipid peroxidation of rat brain induced by oxygen free radical was measured by thiobarbituric acid spectrophotometry. The chelating activities of Bre for Fe 2+ were tested by differential spectrum. Superoxide anion (O 2)from xanthine xanthine oxidase (Xan XO) system and hydroxyl radical (·OH) from FeSO 4 H 2O 2 system were determined with spectrophotometry. It was found that Bre could effectively inhibit the lipid peroxidation of brain mitochondria induced by free radicals driven from Xan XO and FeSO 4 H 2O 2 system. The IC 50 of Bre were 93 01 μmol·L -1 for Xan XO system and 62 18 μmol·L -1 for FeSO 4 H 2O 2 system. Bre also scavenged O 2 and ·OH produced by Xan XO and FeSO 4 H 2O 2 systems. The IC 50 of Bre were 32 63 μmol·L -1 for O - 2 and 20 22 μmol·L -1 for ·OH. Furthermore, the chelating Fe 2+ activity of Bre was shown. It may be concluded that Bre inhibited lipid peroxidation at different stages of the reaction of oxygen free redial with the mitochondria membrane: (1) the formation of ·OH; (2) the initiation of the lipid peroxidation, by chelating Fe 2+ and scavenging O 2 as well as ·OH. The scavenging oxygen free radicals and chelating iron are the mechanisms of inhibitory effect of Bre on lipid peroxidation. 展开更多
关键词 breviscapinE Brain mitochondria Lipid peroxidation Oxygen free radical CHELATOR
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Therapeutic effect and mechanism of breviscapine on cisplatin-induced nephrotoxicity in mice 被引量:6
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作者 Xiao-Yu Lou Jing-Liang Cheng Bo Zhang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2015年第10期853-857,共5页
Objective:To observe the protective effect of breviscapineon mice with cisplatin-induced nephrotoxicity.Methods:Mice were given a single injection of cisplalin(8 mg/kg,up.);then,breviscapine was given to mice at 25 mg... Objective:To observe the protective effect of breviscapineon mice with cisplatin-induced nephrotoxicity.Methods:Mice were given a single injection of cisplalin(8 mg/kg,up.);then,breviscapine was given to mice at 25 mg/kg and 50 mg/kg doses,respectively,once a day for seven days.Renal tissue structure was observed after animals were sacrificed.Blood urea nitrogen(BUN),serum creatinine(Scr),lipid peroxide(MDA) and superoxide dismutase(SOD) serum levels were detected;and MDA,glutathione peroxidase,and SOD levels in the renal cortex were detected.Results:Compared with the blank control group(BCG),the kidney pathological damage of mice in the model control group(MCG) was more severe.After applying different doses of breviscapine,different degrees of renal injury improvement appeared.Compared with the BCG,the serum levels of Scr and BUN in the MCG increased to(89.92±6.78) μmoL/L and(15.32±4.53) mmoL/L.The differences were statistical significant(P<0.01).Compared with the MCG,the serum levels of Scr and BUN in the Bre low-dose groups and Bre high-dose groups decreased significantly(P<0.05).Compared with the BCG,the MDA levels in serum and in the renal cortex in the MCG significantly increased,while the SOD levels significantly decreased.Both the differences were statistically significant(P<0.01).In the Bre low-dose groups and Bre high-dose groups,MDA levels in serum and in the renal cortex significantly decreased,while SOD and glutathione peroxidase levels in the renal cortex significantly increased,compared with the MCG;and the differences were statistically significant(P<0.05).Conclusions:Breviscapine can reduce cisplatin induced renal toxicity in mice and it's possible through inhibition of renal tubule cell lipid peroxidation and reduces the nephrotoxicity of cisplatin. 展开更多
关键词 CISPLATIN RENAL INJURY breviscapinE Protective eff
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Breviscapine attenuates acute pancreatitis by inhibiting expression of PKCα and NF-κB in pancreas 被引量:11
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作者 Hong Zhang Cui-Zhu Cai +5 位作者 Xiao-Qin Zhang Tao Li Xiao- Yun Jia Bao-Lan Li Liang Song Xiao-Jun Ma 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第14期1825-1830,共6页
AIM:To study the effect of breviscapine (Bre) on activity of protein kinase Cα (PKCα) and nuclear factor (NF)-κB in pancreas,and the mechanism of Bre attenuating acute pancreatitis (AP). METHODS:One hundred and eig... AIM:To study the effect of breviscapine (Bre) on activity of protein kinase Cα (PKCα) and nuclear factor (NF)-κB in pancreas,and the mechanism of Bre attenuating acute pancreatitis (AP). METHODS:One hundred and eight rats were randomly divided into acute necrotizing pancreatitis (ANP) group,Bre group (ANP + Bre group) and sham operation (SO) group,36 rats in each group. ANP model was induced by a retrograde injection of 4% sodium deoxycholate into the bilio-pancreatic duct. Fifteen minutes after the ANP model was induced,the rats in Bre group were intraperitoneally injected with Bre (0.4 mg/100 g body weight or 0.1 mL/100 g body weight). Survival time and mortality of rats were calculated. Serum amylase and malondialdehyde levels were measured,volume of ascites was recorded and morphology of pancreas and lung was evaluated at 1,5 and 10 h,after the ANP model was induced,respectively. Expressions of PKCα and subunit p65 of NF-κB in pancreas were detected by immunohistochemistry and Western blotting. RESULTS:The life span of rats was longer and the mortality was lower in Bre group than in ANP group 13.51 ± 5.46 vs 25.36 ± 8.11 (P < 0.05). The amylase and MDA levels as well as the volume of ascites were lower and the pathological changes in pancreas and lung were less in Bre group than ANP group (P < 0.05),indicating that the pancreatitis is less severe in Bre group than ANP group. The activation of PKCα and NF-κB p65 in pancreas was induced rapidly and reached their peak at 1 h or 5 h after ANP,but their activity in Bre group was significantly inhibited. CONCLUSION:Bre exerts its therapeutic effect on AP by inhibiting the activation of PKCα and NF-κB p65 in pancreas. 展开更多
关键词 breviscapinE Acute pancreatitis Protein kinase Nuclear factor-κB RAT
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Breviscapine alleviates hepatic injury and inhibits PKC-mRNA and its protein expression in brain-dead BA-Ma mini pigs 被引量:3
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作者 Zhang, Shui-Jun Song, Yan +4 位作者 Zhai, Wen-Long Shi, Ji-Hua Feng, Liu-Shun Zhao, Yong-Fu Chen, Shi 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2007年第6期604-609,共6页
BACKGROUND: Brain-dead donors are the main sources for organ transplantation, but many studies show that brain-death affects the organ's function after transplantation. This study was undertaken to investigate liv... BACKGROUND: Brain-dead donors are the main sources for organ transplantation, but many studies show that brain-death affects the organ's function after transplantation. This study was undertaken to investigate liver injury after brain-death in BA-Ma mini pigs and the protective effects of breviscapine on hepatic function and on PKC-alpha mRNA and its protein expression. METHODS: Fifteen BA-Ma mini pigs were equally divided into 3 groups at random: brain-dead (group B), breviscapine pretreated (group P), and control (group Q. The brain-dead model was established by increasing intracranial pressure in a modified, slow and intermittent way. At 3, 6, 12, 18 and 24 hours after the initial brain-death, the levels of serum AST, ALT, TNF-alpha, IL-1 beta, and IL-6 were determined. The changes in hepatic tissues were assessed, and the expression of PKC-alpha and PKC-alpha mRNA was detected by immunohistochemistry and RTPCR, respectively. RESULTS: The levels of AST and ALT in groups B and P began to increase 12 hours after brain-death, while the values in group P were lower than those in group B (P<0.05). The levels of IL-1 beta, IL-6, and TNF-alpha in groups B and P at 3, 6, 12 and 18 hours were lower than those in group B (P<0.05). At 6, 12 and 24 hours, the expressions of PKC-a mRNA and PKC-a protein in group P were lower than those in group B (P<0.05). The degree of injury to hepatic cells in group P was milder than that in group B. CONCLUSIONS: Breviscapine inhibits the degree of PKC-alpha mRNA transcription and its protein translation, decreases the release of inflammatory factors, and thus alleviates hepatic injury during brain-death. 展开更多
关键词 breviscapinE BA-Ma mini pigs brain-death protein kinase C
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Breviscapine reduces neuronal injury caused by traumatic brain injury insult:partly associated with suppression of interleukin-6 expression 被引量:16
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作者 Ling Jiang Yue Hu +3 位作者 Xiang He Qiang Lv Ting-hua Wang Qing-jie Xia 《Neural Regeneration Research》 SCIE CAS CSCD 2017年第1期90-95,共6页
Breviscapine,extracted from the herb Erigeron breviscapus,is widely used for the treatment of cardiovascular diseases,cerebral infarct,and stroke,but its mechanism of action remains unclear.This study established a ra... Breviscapine,extracted from the herb Erigeron breviscapus,is widely used for the treatment of cardiovascular diseases,cerebral infarct,and stroke,but its mechanism of action remains unclear.This study established a rat model of traumatic brain injury induced by controlled cortical impact,and injected 75 μg breviscapine via the right lateral ventricle.We found that breviscapine significantly improved neurobehavioral dysfunction at 6 and 9 days after injection.Meanwhile,interleukin-6 expression was markedly down-regulated following breviscapine treatment.Our results suggest that breviscapine is effective in promoting neurological behavior after traumatic brain injury and the underlying molecular mechanism may be associated with the suppression of interleukin-6. 展开更多
关键词 nerve regeneration breviscapine traumatic brain injury neuroprotective effect interleukin-6 neural regeneration
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Clinical Observation on Breviscapine in Treating Hypertension Patients Complicated with Micro-albuminuria of Renal Impairment 被引量:3
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作者 卫苓 谭劼 《Chinese Journal of Integrated Traditional and Western Medicine》 2005年第1期31-33,共3页
Objective: To evaluate the efficacy of Breviscapine on essential hypertension (EH) patients complicated with micro-albuminuria of renal impairment. Methods: Seventy-six EH patients were randomly assigned to the contro... Objective: To evaluate the efficacy of Breviscapine on essential hypertension (EH) patients complicated with micro-albuminuria of renal impairment. Methods: Seventy-six EH patients were randomly assigned to the control group and the treated group, the former was given amlodipine, captopril/uropidil and the latter was given in addition Breviscapine intravenously dripped for 2 treatment courses. The indexes of serum creatinine (Cr), blood urea nitrogen (BUN), blood and urinary β 2-microglobulin (β 2-MG), and quantitative determination of 24 hrs urinary protein were evaluated before and after treatment. Results: In the control group, compared with before treatment, the quantitative determination of 24 hrs urinary protein got reduced significantly ( P <0.05), while in the treated group, both urinary β 2-MG and quantitative determination of 24 hrs urinary protein got lowered significantly ( P <0.05 and P <0.01). But after treatment, compared with the control group, urinary β 2-MG and quantitative determination of 24 hrs urinary protein in the treated group were obviously reduced ( P <0.05). Conclusion: Besides lowering blood pressure effectively, Breviscapine could improve the renal function significantly and reduce the urinary micro-albuminuria, hence showing promising effect on renal protection. 展开更多
关键词 breviscapinE essential hypertension micro-albuminuria renal impairment
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THE EFFECTS OF BREVISCAPIN ON AT-Ⅲ ACTIVITY, tPA AND PAI IN DOGS DURING ACUTE MYOCARDIAL ISCHEMIA
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作者 盛净 徐济民 +3 位作者 杨菊贤 黄震华 王健 徐伟人 《Medical Bulletin of Shanghai Jiaotong University》 CAS 1995年第2期69-73,共5页
THEEFFECTSOFBREVISCAPINONAT-ⅢACTIVITY,tPAANDPAIINDOGSDURINGACUTEMYOCARDIALISCHEMIAShengJing(盛净),XuJimin(徐济民)... THEEFFECTSOFBREVISCAPINONAT-ⅢACTIVITY,tPAANDPAIINDOGSDURINGACUTEMYOCARDIALISCHEMIAShengJing(盛净),XuJimin(徐济民),YangJuxian(杨菊贤),... 展开更多
关键词 breviscapin MYOCARDIAL ISCHEMIA AT- ACTIVITY TPA PAL
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EFFECTS OF BREVISCAPIN ON MYOCARDIAL REOXYGENATION DAMAGE: AN EXPERIMENTAL STUDY
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作者 解玉水 徐济民 +1 位作者 朱菊红 周礼明 《Medical Bulletin of Shanghai Jiaotong University》 CAS 1992年第1期27-31,共5页
By means of Langendor ff isolated-heart perfusion technique, a model of cardiac global hypoxia/reoxygenation was prepared. Breviscapin (100mg/L in perfusate) effectively reduced the myocardial hypoxic perfusion and re... By means of Langendor ff isolated-heart perfusion technique, a model of cardiac global hypoxia/reoxygenation was prepared. Breviscapin (100mg/L in perfusate) effectively reduced the myocardial hypoxic perfusion and reoxygenation damage with respect to lowering myocardial CPK release, indicating a membrane-protective effect of the drug. After 50min hypoxic perfusion. Breviscapin-treated grup showed relatively higher myocardial activity of superoxide dismutase and glutathione peroxidase comparing to the control group. After 5min reoxygenation, the level of myocardial malondialdehyde-like substance in the treated group was lower than that in the control group indicating less free radical accumulated in the myocardium of treated group. 展开更多
关键词 breviscapin REOXYGENATION free RADICAL
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Analysis on Common Compatibility Contraindications of Breviscapine for Injection
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作者 Chenggen ZHAO Hongbo ZHANG Fei HOU 《Medicinal Plant》 CAS 2021年第6期60-66,共7页
[Objectives]To analyze the common compatibility contraindications of breviscapine for injection,and to provide references for clinical rational drug use.[Methods]The pH distribution of the combined drugs in the report... [Objectives]To analyze the common compatibility contraindications of breviscapine for injection,and to provide references for clinical rational drug use.[Methods]The pH distribution of the combined drugs in the report on the compatibility contraindications of breviscapine for injection and was analyzed.[Results]Breviscapine for injection may become turbid or precipitated when mixed with drugs whose pH are lower;it can make the liquid discoloration in a strong alkaline solution.[Conclusions]Breviscapine for injection should not be combined with drugs whose pH are lower,especially drugs with pH lower than 4.2.Breviscapine for injection should not be used with drugs with strong alkaline.It is recommended to use Breviscapine for injection separately. 展开更多
关键词 breviscapine for injection Compatibility contraindications PH Raditional drug use ANALYSIS
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Analysis of 8611 Cases of Adverse Reaction Reports of Breviscapine for Injection
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作者 Hongbo ZHANG Chenggen ZHANG Fei HOU 《Medicinal Plant》 CAS 2022年第1期44-51,共8页
[Objectives]To analyze the occurrence rules and factors influencing adverse reactions of breviscapine for injection,explore potential drug risks,and guide the clinical rational medication.[Methods]The retrospective an... [Objectives]To analyze the occurrence rules and factors influencing adverse reactions of breviscapine for injection,explore potential drug risks,and guide the clinical rational medication.[Methods]The retrospective analysis method was used to analyze the case reports of adverse reactions of breviscapine for injection,and analyze the gender and age distribution of the cases,the patient's medication status,the adverse reactions involving organ/system damage and clinical manifestations,the occurrence time,duration,and outcome of the adverse reactions.[Results]Adverse reactions of breviscapine for injection were mainly concentrated in middle-aged and elderly patients aged 45 and above,accounting for 85.35%;in the gender distribution,females were higher than males;adverse reactions involved multiple organ/system damages.Among them,75.86%of patients had adverse reactions after the first medication,and 11.77%of reported patients had concomitant medications.[Conclusions]The adverse reactions caused by breviscapine for injection may be related to the patient's age,gender and irrational medication. 展开更多
关键词 breviscapine for injection Adverse reaction ANALYSIS
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灯盏花素对心肌缺血再灌注损伤大鼠的心肌保护作用 被引量:1
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作者 孙玉艳 杨然 高丽华 《西北药学杂志》 2024年第1期49-54,共6页
目的观察灯盏花素对心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)大鼠的心肌保护作用,并探讨其可能的机制。方法将60只SD大鼠随机分为健康组、模型组、灯盏花素低剂量和灯盏花素高剂量组,各15只;灯盏花素低剂量、... 目的观察灯盏花素对心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)大鼠的心肌保护作用,并探讨其可能的机制。方法将60只SD大鼠随机分为健康组、模型组、灯盏花素低剂量和灯盏花素高剂量组,各15只;灯盏花素低剂量、灯盏花素高剂量组大鼠腹腔注射灯盏花素(50、100 mg·kg^(−1));健康组、模型组大鼠腹腔注射等体积生理盐水。每日1次,干预5 d。模型组、灯盏花素低剂量组、灯盏花素高剂量组最终均纳入10只大鼠。检测心电图指标;2,3,5-氯化三苯基四氮(2,3,5-tri⁃phenyltetrazolium chlorid,TTC)染色检测心肌梗死面积;检测血清心肌损伤指标;检测血清氧化应激指标;检测血清炎性因子水平;蛋白质印迹法(Western blotting)检测心肌组织白细胞介素-23(interleukin-23,IL-23)、白细胞介素-17(interleukin-17,IL-17)蛋白的表达水平。结果与模型组比较,灯盏花素低剂量组大鼠室颤(ventricular fibrillation,VF)和室性心动过速(ventricular tachycardia,VT)发生次数、血清肌酸激酶同工酶(creatine kinase isoenzyme,CK-MB)活性、心肌肌钙蛋白T(cardiac troponin T,cTnT)活性、丙二醛(malondialdehyde,MDA)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、心肌组织IL-23及IL-17蛋白的表达水平降低,VF、VT持续时间缩短,血清超氧化物歧化酶(superoxide dismutase,SOD)活性升高(P<0.05);灯盏花素低剂量组和灯盏花素高剂量组各指标水平变化规律相同,灯盏花素变化高剂量组变化更显著(P<0.05)。结论灯盏花素可缓解MIRI大鼠心律失常、减轻心肌损伤及氧化应激反应和炎症反应。推测其作用机制可能与抑制IL-23/IL-17轴活性有关。 展开更多
关键词 灯盏花素 心肌缺血再灌注损伤 白细胞介素-23 白细胞介素-17
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灯盏花素对肝纤维化大鼠的干预作用及机制
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作者 魏丹丹 李闪闪 +9 位作者 张明昊 魏雨润 王红玲 柴爽爽 殷晶晶 张敏 赵菡 吴宗耀 朱奎成 王庆波 《中国药房》 CAS 北大核心 2024年第6期671-677,共7页
目的基于转化生长因子β1(TGF-β1)/Smad2/胞外信号调节激酶1(ERK1)通路和Kelch样环氧氯丙烷相关蛋白1(Keap1)/核转录因子红系2相关因子2(Nrf2)/血红素加氧酶1(HO-1)通路,探讨灯盏花素对肝纤维化(HF)大鼠的干预作用及潜在机制。方法将6... 目的基于转化生长因子β1(TGF-β1)/Smad2/胞外信号调节激酶1(ERK1)通路和Kelch样环氧氯丙烷相关蛋白1(Keap1)/核转录因子红系2相关因子2(Nrf2)/血红素加氧酶1(HO-1)通路,探讨灯盏花素对肝纤维化(HF)大鼠的干预作用及潜在机制。方法将60只大鼠随机分为正常对照组,模型组,灯盏花素低、中、高剂量组(5.4、10.8、21.6 mg/kg)和秋水仙碱组(阳性对照,0.45 mg/kg),每组10只,雌雄各半。除正常对照组外,其余各组大鼠均以四氯化碳诱导构建HF模型。随后,各药物组大鼠灌胃相应药液,每天1次,连续28 d。观察各组大鼠的肝脏外观并计算其肝脏系数,检测其血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平和肝组织中ALT、AST、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)水平,观察其肝组织炎症和纤维化情况,检测肝组织中TGF-β1、Smad、ERK1、Nrf2、Keap1、HO-1蛋白及mRNA的表达情况。结果与正常对照组比较,模型组大鼠肝脏可见大面积的白色结节灶、明显的炎症细胞浸润和胶原纤维沉积;其体重,肝组织中SOD、GSH-Px水平和Nrf2、HO-1蛋白及mRNA的表达水平均显著降低(P<0.05);肝脏系数,Masson染色阳性面积百分比,血清及肝组织中ALT、AST水平,肝组织中MDA水平和TGF-β1、Smad2、ERK1、Keap1蛋白及mRNA的表达水平显著升高(P<0.05)。与模型组比较,各药物组大鼠肝组织病变均有所改善,上述定量指标普遍逆转(P<0.05)。结论灯盏花素对大鼠HF有较好的干预作用,其作用可能与抑制TGF-β1/Smad2/ERK1通路来抗纤维化,调控Keap1/Nrf2/HO-1通路来抑制氧化应激有关。 展开更多
关键词 灯盏花素 肝纤维化 氧化应激 转化生长因子β1/Smad2/胞外信号调节激酶1通路 Kelch样环氧氯丙烷相关蛋白1/核转录因子红系2相关因子2/血红素加氧酶1通路
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灯盏花素对普伐他汀大鼠体内转运过程影响的机制研究
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作者 鞠爱霞 周育生 +3 位作者 胡勇 郄青松 刘莉 李秋红 《中医药学报》 CAS 2024年第3期40-46,共7页
目的:探究灯盏花素对大鼠体内普伐他汀转运过程影响的作用机制,为临床合理用药提供数据支撑。方法:正常SD大鼠24只,随机分为生理盐水组、普伐他汀组、灯盏花素组和普伐他汀+灯盏花素组,每组6只。正常KM小鼠60只,随机分为普伐他汀组和普... 目的:探究灯盏花素对大鼠体内普伐他汀转运过程影响的作用机制,为临床合理用药提供数据支撑。方法:正常SD大鼠24只,随机分为生理盐水组、普伐他汀组、灯盏花素组和普伐他汀+灯盏花素组,每组6只。正常KM小鼠60只,随机分为普伐他汀组和普伐他汀+灯盏花素组,每组30只。按照不同剂量给药后采集大鼠胆汁样品和小鼠组织样品处理,采用高效液相色谱法测定样品中普伐他汀的药物浓度;采用RT-PCR和WB技术检测单独及联合给药对大鼠肝脏中Mrp2转运体基因表达和蛋白表达水平的影响。结果:与普伐他汀组比较,普伐他汀+灯盏花素组中除脑组织以外各组织内普伐他汀的药物浓度显著增加(P<0.05);普伐他汀的胆汁分泌量明显降低(P<0.01);与生理盐水组比较,普伐他汀组Mrp2基因和蛋白表达均无明显变化(P>0.05),灯盏花素组及普伐他汀+灯盏花素组中Mrp2转运体基因表达量明显下降(P<0.05),蛋白含量略有减少,但差异无统计学意义(P>0.05)。结论:联用后,灯盏花素可能通过竞争抑制Mrp2转运体功能,使普伐他汀外排转运减慢,体内药物浓度增加,进而提高普伐他汀的临床疗效。 展开更多
关键词 灯盏花素 普伐他汀 Mrp2转运体
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灯盏花素对急性心肌梗死大鼠的心肌保护作用及机制研究
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作者 李浩亮 许邦龙 +1 位作者 胡广全 高峰 《安徽医专学报》 2024年第3期78-81,共4页
目的:探讨灯盏花素对急性心肌梗死(AMI)大鼠的心肌保护作用及其分子机制。方法:将32只SD大鼠随机均分为假手术组、AMI组、AMI+灯盏花素组,AMI+灯盏花素+LY294002组。通过超声心动图、TUNEL染色、ELISA、Western blot和qRT-PCR等多种检... 目的:探讨灯盏花素对急性心肌梗死(AMI)大鼠的心肌保护作用及其分子机制。方法:将32只SD大鼠随机均分为假手术组、AMI组、AMI+灯盏花素组,AMI+灯盏花素+LY294002组。通过超声心动图、TUNEL染色、ELISA、Western blot和qRT-PCR等多种检测方法,探究各组大鼠的心功能、心肌细胞凋亡率、心肌损伤程度、凋亡相关分子以及PI3K/Akt信号通路的表达情况。结果:与假手术组比较,AMI组大鼠的心脏收缩功能降低,心肌细胞凋亡率显著增加,心肌损伤严重;与AMI组比较,AMI+灯盏花素组的上述病理改变明显减轻;当加入PI3K的特异性抑制剂LY294002后,灯盏花素对AMI大鼠的治疗作用被削弱。结论:灯盏花素可能是通过激活PI3K/Akt信号通路抑制心肌细胞凋亡,从而减轻AMI导致的大鼠心肌损伤,改善心功能。 展开更多
关键词 灯盏花素 急性心肌梗死 PI3K/AKT信号通路 细胞凋亡
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灯盏花素穴位注射联合针灸对腰椎间盘突出症患者腰背伸肌群表面肌电指标及血清MMP3、PGE_(2)、IL-1β表达的作用 被引量:2
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作者 刘元朗 刘志杰 朱宏飞 《中华中医药学刊》 CAS 北大核心 2024年第5期165-169,共5页
目的探讨灯盏花素穴位注射联合针灸对腰椎间盘突出症(Lumbar disc herniation,LDH)患者腰背伸肌群表面肌电指标及血清基质金属蛋白酶3(Matrix metalloproteinase 3,MMP3)、前列腺素E2(Prostaglandin E_(2),PGE_(2))、白细胞介素-1β(Int... 目的探讨灯盏花素穴位注射联合针灸对腰椎间盘突出症(Lumbar disc herniation,LDH)患者腰背伸肌群表面肌电指标及血清基质金属蛋白酶3(Matrix metalloproteinase 3,MMP3)、前列腺素E2(Prostaglandin E_(2),PGE_(2))、白细胞介素-1β(Interleukin-1β,IL-1β)表达的作用。方法应用前瞻性随机对照研究方法选取2020年3月—2022年5月收治的LDH患者186例,以随机数字表法分为观察组(62例)、对照A组(62例)、对照B组(62例)。对照A组予以灯盏花素穴位注射,对照B组予以针灸,观察组予以灯盏花素穴位注射联合针灸,均治疗1个月。比较3组疗效、不良反应与治疗前、治疗1个月后疼痛程度评分(Visual Analogue Scale/Score,VAS)、日本骨科协会下腰痛功能评价表评分(Japanese Orthopaedic Association Scores,JOA)、日常生活能力评分(Barthel,BI)、腰背伸肌群表面肌电指标(积分肌电值、平均功率频率)、血液流变学指标、血清免疫功能指标[免疫球蛋白G(Immunoglobulin G,IgG)、免疫球蛋白M(Immunoglobulin M,IgM)、补体C_(3)]、MMP3、PGE_(2)、IL-1β水平。结果观察组治疗1个月后总有效率93.55%(58/62)高于对照A组7742%(48/62)、对照B组80.65%(50/62)(P<0.05);3组治疗1个月后VAS评分较治疗前降低,且观察组低于对照A组、对照B组,JOA、BI评分与积分肌电值、平均功率频率较治疗前增高,且观察组高于对照A组、对照B组(P<0.05);3组治疗1个月后全血低切黏度、全血高切黏度、血浆黏度、红细胞压积、红细胞聚集指数较治疗前降低,且观察组低于对照A组、对照B组(P<0.05);3组治疗1个月后血清IgG、IgM、补体C_(3)、MMP3、PGE_(2)、IL-1β水平较治疗前降低,且观察组低于对照A组、对照B组(P<0.05);3组治疗期间不良反应发生率相比,差异无统计学意义(P>0.05)。结论应用灯盏花素穴位注射联合针灸治疗LDH可缓解疼痛程度与腰背伸肌群疲劳程度,提升腰背伸肌群肌肉收缩性,改善腰椎功能及日常生活能力,改善血液流变学、免疫功能,下调血清MMP3、PGE_(2)、IL-1β表达,疗效显著,且安全性好。 展开更多
关键词 灯盏花素 穴位注射 针灸 腰椎间盘突出症 腰背伸肌群表面肌电指标 MMP3 PGE_(2) IL-1Β
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Effect of breviscapine on fractalkine expression in chronic hypoxic rats 被引量:3
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作者 CHEN Xiao-ju CHENG De-yun YANG Li XIA Xiu-qiong GUAN Jian 《Chinese Medical Journal》 SCIE CAS CSCD 2006年第17期1465-1468,共4页
Fractalkine (FKN) is the only known chemokine that fulfils the dual functions of an adhesive molecule and a soluble chemoattractant.1 FKN expression was reported increase in lungs of patients with severe pulmonary a... Fractalkine (FKN) is the only known chemokine that fulfils the dual functions of an adhesive molecule and a soluble chemoattractant.1 FKN expression was reported increase in lungs of patients with severe pulmonary arterial hypertension,2 suggesting that FKN may participate in pathogenesis of pulmonary hypertension. Breviscapine is a flavonoid extracted from Erigeron breviscapus (Vant.) Hand. Mazz. Breviscapine can prevent the development of hypoxic pulmonary hypertension^3 but the mechanism is unknown. This study evaluated the role of FKN in the pathogenesis of hypoxic pulmonary hypertension and the effect of breviscapine on FKN in hypoxic pulmonary hypertension. 展开更多
关键词 HYPOXIA pulmonary hypertension FRACTALKINE breviscapinE
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基于网络药理学探究灯盏花素联用三七总皂苷治疗脑梗死的潜在作用机制
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作者 张鑫鑫 周越 +3 位作者 张世诚 李雪 沈金阳 刘强 《广东化工》 CAS 2024年第17期60-63,共4页
基于网络药理学方法探究灯盏花素联用三七总皂苷治疗脑梗死(cerebral infarction,CI)的潜在作用机制。运用PubChem以及Swiss Target Prediction数据库获取灯盏花素(breviscapine)和三七总皂苷(panax notoginseng saponins,PNS)的主要活... 基于网络药理学方法探究灯盏花素联用三七总皂苷治疗脑梗死(cerebral infarction,CI)的潜在作用机制。运用PubChem以及Swiss Target Prediction数据库获取灯盏花素(breviscapine)和三七总皂苷(panax notoginseng saponins,PNS)的主要活性成分以及相关靶点。利用GeneCards、DisGeNET、Drugbank和PharmGkb数据库收集的相关疾病靶点,与成分靶点取交集绘制维恩图。在STRING平台获取蛋白-蛋白相互作用(protein-protein interaction,PPI)网络,采用Cytoscape3.7.2软件CytoNCA插件构建网络图并进行可视化处理。采用DAVID数据库进行基因本体论(Gene Ontology,GO)生物过程和京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析。有效成分6种,共检测到42个有效成分靶点,CI相关靶点960个,取交集后得到灯盏花素和三七总皂苷治疗CI的潜在靶点15个。对交集靶点富集,GO富集分析共涉及生物过程111个,细胞组分19个,分子功能20个,KEGG通路富集得到55条通路,结合蛋白-蛋白相互作用网络分析与富集分析最终得到TNF、AKT1、EGFR、PTGS2、STAT3共5个核心靶点。本研究初步探索了灯盏花素联用三七总皂苷治疗脑梗死的作用机制为后续研究提供基础。 展开更多
关键词 网络药理学 灯盏花素 三七总皂苷 联用 脑梗死
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加贝酯联合灯盏花素注射液治疗急性胰腺炎的临床效果
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作者 高新生 《中国医学创新》 CAS 2024年第6期5-10,共6页
目的:探究加贝酯联合灯盏花素注射液治疗急性胰腺炎的临床效果。方法:选取2021年1月—2023年1月湖北科技学院附属第二医院收治的急性胰腺炎患者80例,以随机数字表法分为两组,对照组(加贝酯)及观察组(加贝酯联合灯盏花素注射液),各40例... 目的:探究加贝酯联合灯盏花素注射液治疗急性胰腺炎的临床效果。方法:选取2021年1月—2023年1月湖北科技学院附属第二医院收治的急性胰腺炎患者80例,以随机数字表法分为两组,对照组(加贝酯)及观察组(加贝酯联合灯盏花素注射液),各40例。对比两组临床症状改善时间(腹痛消失时间、腹胀消失时间、排便正常时间、排气正常时间)、肠黏膜功能[D-乳酸、内毒素、尿乳果糖(L)/甘露醇(M)]、炎症因子[肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)、C反应蛋白(CRP)]、肝功能[天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)]、肾功能[肌酐(Cr)、尿素]、心肌酶[乳酸脱氢酶(LDH)、肌酸激酶(CK)]、Toll样受体4(TLR4)mRNA、核因子κB(NF-κB)mRNA表达量。结果:观察组腹痛消失时间、腹胀消失时间、排便正常时间、排气正常时间均短于对照组,差异均有统计学意义(P<0.05)。治疗前,两组D-乳酸、内毒素、尿L/M水平对比,差异均无统计学意义(P>0.05);治疗后,两组D-乳酸、内毒素、尿L/M水平均下降,观察组均低于对照组,差异均有统计学意义(P<0.05)。治疗前,两组TNF-α、IL-6、CRP水平对比,差异均无统计学意义(P>0.05);治疗后,两组TNF-α、IL-6、CRP水平均降低,观察组均低于对照组,差异均有统计学意义(P<0.05)。治疗前,两组AST、ALT、Cr、尿素对比,差异均无统计学意义(P>0.05);治疗后,两组AST、ALT、Cr、尿素均下降,观察组均低于对照组,差异均有统计学意义(P<0.05)。治疗前,两组心肌酶指标、TLR4 mRNA、NF-κB mRNA水平对比,差异均无统计学意义(P>0.05);治疗后,两组LDH、CK、TLR4 mRNA、NF-κB mRNA水平均下降,观察组均低于对照组,差异均有统计学意义(P<0.05)。结论:急性胰腺炎患者采用加贝酯联合灯盏花素注射液治疗效果显著,可降低炎症因子,改善肠黏膜功能,抑制TLR4 mRNA、NF-κB mRNA通路及有关因子表达,保护肝、肾、心功能。 展开更多
关键词 加贝酯 灯盏花素注射液 急性胰腺炎 炎症因子 肝、肾功能 心肌酶
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灯盏乙素通过细胞转运途径对阿尔茨海默病大鼠β-淀粉样蛋白跨血脑屏障的影响
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作者 李可 苗果 +1 位作者 柳秋 赵昆朋 《中西医结合心脑血管病杂志》 2024年第10期1782-1786,共5页
目的:探讨灯盏乙素对阿尔茨海默病(AD)大鼠β-淀粉样蛋白(Aβ)跨血脑屏障(BBB)的影响及其机制。方法:无特定病原体(SPF)级SD大鼠70只,随机分为假手术组、模型组、灯盏乙素低剂量组、灯盏乙素高剂量组以及阳性对照组,除假手术组大鼠外,... 目的:探讨灯盏乙素对阿尔茨海默病(AD)大鼠β-淀粉样蛋白(Aβ)跨血脑屏障(BBB)的影响及其机制。方法:无特定病原体(SPF)级SD大鼠70只,随机分为假手术组、模型组、灯盏乙素低剂量组、灯盏乙素高剂量组以及阳性对照组,除假手术组大鼠外,其余组大鼠侧脑室注射Aβ_(1-42)氯化钠溶液建立AD大鼠模型,灯盏乙素低剂量和高剂量组大鼠分别灌胃灯盏乙素5 mg/kg和10 mg/kg,阳性对照组灌胃盐酸多奈哌齐0.9 mg/kg,持续30 d,观察大鼠行为能力以及血脑屏障通透性,进行苏木精-伊红(HE)染色和免疫组化染色,蛋白质免疫印迹法(Western Blot)检测基质金属蛋白酶-9(MMP-9)、闭合蛋白-5(Claudin-5)、咬合蛋白(Occludin)、低密度脂蛋白相关蛋白(LRP-1)以及p糖蛋白(P-gp)表达情况。结果:模型组神经元细胞排列疏松、紊乱,细胞数量减少,可见明显空泡化,灯盏乙素低剂量组、灯盏乙素高剂量组及阳性对照组神经元细胞数量增多,空泡化细胞减少,细胞排列稍整齐致密。灯盏乙素低剂量组、灯盏乙素高剂量组及阳性对照组大鼠逃避潜伏期、伊文思蓝含量、Aβ斑块面积以及MMP-9蛋白相对表达量均低于模型组,穿过平台的次数以及LRP-1、P-gp、Claudin-5、Occludin蛋白相对表达量高于模型组(P<0.05)。结论:灯盏乙素可减轻AD大鼠神经元病理损伤,保护血脑屏障。 展开更多
关键词 阿尔茨海默病 血脑屏障 Β-淀粉样蛋白 灯盏乙素 实验研究
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