Sturgeons are the most important principal market species in the Caspian Sea. In the present study, measurement of blood parameters was carried out with the aim of evaluating the normal value of hematological and seru...Sturgeons are the most important principal market species in the Caspian Sea. In the present study, measurement of blood parameters was carried out with the aim of evaluating the normal value of hematological and serum biochemical parameters of brood stocks Acipenser persicus during spawning season. Blood samples were collected from 36 brood stocks of A. persicus（18 males and 18 females） during the spawning season. Higher value of red blood cells, white blood cells, haemoglobin, haematocrit was observed in male breeders with significant differences between female breeders（p〉0.05）. Haemoglobin and haematocrit value in this study was within the range of 8.70 to 9.2 g/d L and 29.73% to 30.26%, respectively. The statistically significant differences between mean corpuscular volumes, mean corpuscular haemoglobin, lymphocyte and neutrophil percentages, aspartate aminotransferase, alkaline phosphatase and creatine kinase were observed in the male and female brood stocks.It is concluded from this study, some of the hematological and serum biochemical parameters of male and female A. persicus breeders were significantly varied from each other. In comparative investigation with earlier reports,the values of red blood cells, haemoglobin and haematocrit are highly varied due to age variations of fish.However, further studies are necessary to understand the impact of sexual, age, size, length, and season and habitat variation. Serum biochemical parameters can be used for confirming the maturity of A. persicus and monitoring any changes in the water quality parameters and soil.展开更多
利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OIQ、SISQ、Kona Bay Q)及其子一代(OIZ、SISZ、Kona Bay Z)共计120个个体进行遗传分析。结果表明,8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数...利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OIQ、SISQ、Kona Bay Q)及其子一代(OIZ、SISZ、Kona Bay Z)共计120个个体进行遗传分析。结果表明,8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数为2～6个,平均等位基因数为3.5;各位点的期望杂合度均比观测杂合度高;多态信息含量(PIC)值为0.4794～0.7699,说明这8个位点在凡纳滨对虾中具有较高的信息含量。在亲本和子代群体遗传结构分析中,子代的有效等位基因数、杂合度和多态信息含量等指标均略低于亲本群体,但子代的遗传多样性并未受到太大影响,仍保持较好的遗传力。展开更多
Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique...Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.展开更多
The qualitative and quantitative composition of primordial germ cells(PGCs)and the genetic variability of embryos of the Coregonus nasus from the Ob river basin and domesticated population breeding outside the natural...The qualitative and quantitative composition of primordial germ cells(PGCs)and the genetic variability of embryos of the Coregonus nasus from the Ob river basin and domesticated population breeding outside the natural areal was examined.In the broad whitefish,typical,binuclear,polymorphic nuclei cells,as well as PGCs represented primary gonocytes during embryogenesis in clusters.When hatching larvae PGCs mitoses were noted only in domesticated juveniles.The genetic variability of embryos of the broad whitefish was studied by polymerase chain reaction of inter simple sequence repeats(ISSR-PCR).The high polymorphism of the broad whitefish cultured population was revealed.The proportion of polymorphic bands was 89.4%,Nei’s genetic diversity was 0.32.Reproduction of the domesticated broad whitefish did not affect the overall level of polymorphism of neutral DNA markers.These findings suggest the preservation of cytomorphological and genetic diversity in C.nasus embryo from different sources.展开更多
基金supported by Iranian Fisheries Research OrganizationTehran University
文摘Sturgeons are the most important principal market species in the Caspian Sea. In the present study, measurement of blood parameters was carried out with the aim of evaluating the normal value of hematological and serum biochemical parameters of brood stocks Acipenser persicus during spawning season. Blood samples were collected from 36 brood stocks of A. persicus（18 males and 18 females） during the spawning season. Higher value of red blood cells, white blood cells, haemoglobin, haematocrit was observed in male breeders with significant differences between female breeders（p〉0.05）. Haemoglobin and haematocrit value in this study was within the range of 8.70 to 9.2 g/d L and 29.73% to 30.26%, respectively. The statistically significant differences between mean corpuscular volumes, mean corpuscular haemoglobin, lymphocyte and neutrophil percentages, aspartate aminotransferase, alkaline phosphatase and creatine kinase were observed in the male and female brood stocks.It is concluded from this study, some of the hematological and serum biochemical parameters of male and female A. persicus breeders were significantly varied from each other. In comparative investigation with earlier reports,the values of red blood cells, haemoglobin and haematocrit are highly varied due to age variations of fish.However, further studies are necessary to understand the impact of sexual, age, size, length, and season and habitat variation. Serum biochemical parameters can be used for confirming the maturity of A. persicus and monitoring any changes in the water quality parameters and soil.
文摘利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OIQ、SISQ、Kona Bay Q)及其子一代(OIZ、SISZ、Kona Bay Z)共计120个个体进行遗传分析。结果表明,8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数为2～6个,平均等位基因数为3.5;各位点的期望杂合度均比观测杂合度高;多态信息含量(PIC)值为0.4794～0.7699,说明这8个位点在凡纳滨对虾中具有较高的信息含量。在亲本和子代群体遗传结构分析中,子代的有效等位基因数、杂合度和多态信息含量等指标均略低于亲本群体,但子代的遗传多样性并未受到太大影响,仍保持较好的遗传力。
基金Supported by Technology Innovation Projects from Science and Tech-nology Department of Guangdong Province (2008A023700011)~~
文摘Genetic variations of three species from primary parent （OI Q,SIS Q,Kona Bay Q） and their first filial generation （OI Z,SIS Z,Kona Bay Z） of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species;the number of allele of every loci was 2 to 6;mean allele number （Na） was 3.5;expected heterozygosity value （He） were higher than observed heterozygosity value （Ho）;mean polymorphism information content （PIC） ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content （PIC） in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.
文摘The qualitative and quantitative composition of primordial germ cells(PGCs)and the genetic variability of embryos of the Coregonus nasus from the Ob river basin and domesticated population breeding outside the natural areal was examined.In the broad whitefish,typical,binuclear,polymorphic nuclei cells,as well as PGCs represented primary gonocytes during embryogenesis in clusters.When hatching larvae PGCs mitoses were noted only in domesticated juveniles.The genetic variability of embryos of the broad whitefish was studied by polymerase chain reaction of inter simple sequence repeats(ISSR-PCR).The high polymorphism of the broad whitefish cultured population was revealed.The proportion of polymorphic bands was 89.4%,Nei’s genetic diversity was 0.32.Reproduction of the domesticated broad whitefish did not affect the overall level of polymorphism of neutral DNA markers.These findings suggest the preservation of cytomorphological and genetic diversity in C.nasus embryo from different sources.
