We represent assessment of the rats control operator＇s actions, starting from the placement of rat control means （chemical, mechanical and others） in the object territory until the full its elimination and followed...We represent assessment of the rats control operator＇s actions, starting from the placement of rat control means （chemical, mechanical and others） in the object territory until the full its elimination and followed by assessment of the probability of rat population recovery. The probability of success is evaluated when using a combination of rat control means. We took into account changes in rat population occurring in different calendar periods of the year. The proposed calculation method can be used in training programs, as well as for the local forecast of releasing objects from rats and rats＇ re-settling.展开更多
Toll-like receptors(TLRs)are important initiators of innate immune responses that target host-pathogen interactions.However,further research into the molecular characteristics of TLRs in wild populations is required,a...Toll-like receptors(TLRs)are important initiators of innate immune responses that target host-pathogen interactions.However,further research into the molecular characteristics of TLRs in wild populations is required,as well as how TLRs genetically influenced pathogen infections in the brown rat(Rattus norvegicus).Here,we explored the genetic characterization and evolution of 2 sensing nucleic acid TLRs(TLR7 and TLR8)and 2 sensing nonnucleic acid TLRs(TLR2 and TLR4)in the wild brown rat,and assessed their associations with 2 RNA viruses(Seoul hantavirus and rat hepatitis E virus(HEV))and 2 bacteria(Leptospira and Bartonella).In these 4 TLRs,we discovered a total of 16 variants.Furthermore,TLR8 had high genetic diversity among 7 variants,while TLR2 had low genetic diversity with only 1 variant.According to selective pressure analyses,TLR4,TLR7,and TLR8 genes evolved under purifying selection.Interestingly,significant associations were found between 3 TLR8 variants and HEV infection,as well as 1 TLR2 variant and Bartonella infection.Overall,our findings provided a glimpse into the genetic characterization of TLRs in the brown rat,and further demonstrated that TLR2 and TLR8 genetic variations were related to Bartonella and HEV infection,respectively.Especially,TLR8 may be a good candidate immune gene for future research on molecular ecology and functional adaptation in wild populations.展开更多
Objective To analyze the effects of Dan Huang Ming Mu Recipe(DHMMR)(a pharmaceutical preparation from herbs and having the function of replenishing vital essence,removing heat,promoting blood circulation and excreting...Objective To analyze the effects of Dan Huang Ming Mu Recipe(DHMMR)(a pharmaceutical preparation from herbs and having the function of replenishing vital essence,removing heat,promoting blood circulation and excreting pathogenic water) on diabetic retinopathy(DR) after retinal laser photocoagulation through regulating the expression of vascular endothelial growth factor(VEGF) and pigment epithelium-derived factor(PEDF).Methods Forty male Brown Norway(BN) rats were randomly divided into blank group(group A,10 BN rats) and model group(30 BN rats).DR models were induced by 40 mg/kg streptozotocin(STZ) and the body weight and blood glucose of rats were monitored.After 12-week injection,fluorescein fundus angiography(FFA) and histopathological examination were detected to confirm the successful establishment of DR models.Subsequently,the right eyes of model group rats were conducted retinal laser photocoagulation with the left eyes having no retinal laser photocoagulation and rats of the model group were randomly divided into group B(the model control group),group C(the positive control group),and group D(the DHMMR group),with 10 rats in each group.Rats of the group A and the group B were given vehicle,and the group C were given calcium dobesilate suspension by gavage,and the group D were given DHMMR by gavage.After 4-week gavage,FFA was carried out to observe the fundus microvascular change.Then,the rats were sacrificed to do histopathological examinations and the serum levels of P-selectin,cAMP,cGMP,and the relative expression of the protein of VEGF and PEDF in retina were detected.Results After 12-week STZ injection,the blood glucose of the model group were pronouncedly higher than the blank group(P < 0.01).Fundus micro-hemangioma changes were observed in the rats of the model group through FFA,and the rats of the blank group did not see any changes.The pictures of HE staining showed that the retinal structure of the model group was more disordered than the blank group.After 4-week gavage,treatments with DHMMR showed dramatic reduction of serum levels of Pselectin,cAMP and cGMP compared with the group B(P < 0.01).FFA and histopathological examinations of DHMMR-treated rats revealed significantly suppression of retinal edema,fundus microvascular destruction and retinal destruction distinguishing from the group B.And the relative expression of VEGF protein of the group D and the group A was markedly lower than that of the group B(P < 0.01).The relative expression of PEDF protein of the group D and the group A was dramatically higher than that of the group B to the contrary(P < 0.01).Conclusions DHMMR demonstrates pronounced suppressive effects on the progression of DR after retinal laser photocoagulation,through down-regulating VEGF and upregulating PEDF.展开更多
Purpose: Choroidal neovascularization (CNV) plays an important role in pathogenesis of age-related macular degeneration (AMD), ocular histoplasmosis syndrome (OHS) and so on. However, mechanisms of CNV formation are n...Purpose: Choroidal neovascularization (CNV) plays an important role in pathogenesis of age-related macular degeneration (AMD), ocular histoplasmosis syndrome (OHS) and so on. However, mechanisms of CNV formation are not fully understood. The aim of this study is to investigate the correlation between expressions of CD105 and vascular endothelial growth factor (VEGF) in experimental laser-induced CNV in rats. Methods: CNV model was established by 532 nm laser photocoagulation in Brown-Norway rats. The expression of CD105 and VEGF in CNV was observed by immunohistochemistry at 3, 7, 14, 21, 28 and 56 days after laser photocoagulation. The image analysis was performed with the professional software of Image-Pro Plus. Results: Fluorescein angiography showed fluorescein leakage in CNV from days 7 to 56 after photocoagulation. VEGF expression was mainly observed in vascular endothelial cells, ganglion cells, inner nuclear layers and retinal pigment epithelial cells in normal retina and vascular endothelial cells in normal choroid of the rats. On day 3 after photocoagulation, VEGF began to express in laser-induced lesions. VEGF was strongly expressed in CNV after 7 days (P<0.05) and decreased after 14 days (P>0.05). CD105 was initially presented in CNV at 7 days and obviously expressed at 14 days after photocoagulation (P<0.05). Four weeks later, when angiogenesis tended toward inactive status, expression of CD105 was markedly decreased (P>0.05). There was notablely direct correlation between CD105-positive-microvessel density and positively semiquantitative scoring of VEGF in the CNV(r=0.989, P<0.01). Conclusions: There is direct correlation between the expression of CD105 and VEGF in the laser-induced CNV in rat. It suggests that CD105 and VEGF might participate in the new blood vessel formation and promote the growth of CNV.展开更多
文摘We represent assessment of the rats control operator＇s actions, starting from the placement of rat control means （chemical, mechanical and others） in the object territory until the full its elimination and followed by assessment of the probability of rat population recovery. The probability of success is evaluated when using a combination of rat control means. We took into account changes in rat population occurring in different calendar periods of the year. The proposed calculation method can be used in training programs, as well as for the local forecast of releasing objects from rats and rats＇ re-settling.
基金funded by the Major Program of National Natural Science Foundation of China(No.32090023)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA19050204)the National Forestry and Grassland Administration,China,and the Chinese Academy of Sciences(CZBZX-1).
文摘Toll-like receptors(TLRs)are important initiators of innate immune responses that target host-pathogen interactions.However,further research into the molecular characteristics of TLRs in wild populations is required,as well as how TLRs genetically influenced pathogen infections in the brown rat(Rattus norvegicus).Here,we explored the genetic characterization and evolution of 2 sensing nucleic acid TLRs(TLR7 and TLR8)and 2 sensing nonnucleic acid TLRs(TLR2 and TLR4)in the wild brown rat,and assessed their associations with 2 RNA viruses(Seoul hantavirus and rat hepatitis E virus(HEV))and 2 bacteria(Leptospira and Bartonella).In these 4 TLRs,we discovered a total of 16 variants.Furthermore,TLR8 had high genetic diversity among 7 variants,while TLR2 had low genetic diversity with only 1 variant.According to selective pressure analyses,TLR4,TLR7,and TLR8 genes evolved under purifying selection.Interestingly,significant associations were found between 3 TLR8 variants and HEV infection,as well as 1 TLR2 variant and Bartonella infection.Overall,our findings provided a glimpse into the genetic characterization of TLRs in the brown rat,and further demonstrated that TLR2 and TLR8 genetic variations were related to Bartonella and HEV infection,respectively.Especially,TLR8 may be a good candidate immune gene for future research on molecular ecology and functional adaptation in wild populations.
基金the funding support from the Provincial Natural Science Foundation General Program(No.2015JJ2109)Hunan Province Graduate Student Research Innovation Program(No.CX2018B473)+7 种基金Hunan Traditional Chinese Medicine Scientific Research Program(NO.201463 and NO.2017141)Key Scientific Research Project of Hunan Administration of Traditional Chinese Medicine(NO.201917)University-level Scientific Research Projects of Hunan University of Chinese Medicine(NO.2017029 and NO.2018XJJJ31)The Domestic Firstclass Discipline Construction Project of Chinese Medicine of Hunan University of Chinese MedicineCentral Finance Supported Local High School Construction ProjectState Administration of Traditional Chinese Medicine Ophthalmology Key Discipline Construction ProjectHunan Province Traditional Chinese Medicine Facial Feature Key Discipline Construction ProjectHunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine,Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine
文摘Objective To analyze the effects of Dan Huang Ming Mu Recipe(DHMMR)(a pharmaceutical preparation from herbs and having the function of replenishing vital essence,removing heat,promoting blood circulation and excreting pathogenic water) on diabetic retinopathy(DR) after retinal laser photocoagulation through regulating the expression of vascular endothelial growth factor(VEGF) and pigment epithelium-derived factor(PEDF).Methods Forty male Brown Norway(BN) rats were randomly divided into blank group(group A,10 BN rats) and model group(30 BN rats).DR models were induced by 40 mg/kg streptozotocin(STZ) and the body weight and blood glucose of rats were monitored.After 12-week injection,fluorescein fundus angiography(FFA) and histopathological examination were detected to confirm the successful establishment of DR models.Subsequently,the right eyes of model group rats were conducted retinal laser photocoagulation with the left eyes having no retinal laser photocoagulation and rats of the model group were randomly divided into group B(the model control group),group C(the positive control group),and group D(the DHMMR group),with 10 rats in each group.Rats of the group A and the group B were given vehicle,and the group C were given calcium dobesilate suspension by gavage,and the group D were given DHMMR by gavage.After 4-week gavage,FFA was carried out to observe the fundus microvascular change.Then,the rats were sacrificed to do histopathological examinations and the serum levels of P-selectin,cAMP,cGMP,and the relative expression of the protein of VEGF and PEDF in retina were detected.Results After 12-week STZ injection,the blood glucose of the model group were pronouncedly higher than the blank group(P < 0.01).Fundus micro-hemangioma changes were observed in the rats of the model group through FFA,and the rats of the blank group did not see any changes.The pictures of HE staining showed that the retinal structure of the model group was more disordered than the blank group.After 4-week gavage,treatments with DHMMR showed dramatic reduction of serum levels of Pselectin,cAMP and cGMP compared with the group B(P < 0.01).FFA and histopathological examinations of DHMMR-treated rats revealed significantly suppression of retinal edema,fundus microvascular destruction and retinal destruction distinguishing from the group B.And the relative expression of VEGF protein of the group D and the group A was markedly lower than that of the group B(P < 0.01).The relative expression of PEDF protein of the group D and the group A was dramatically higher than that of the group B to the contrary(P < 0.01).Conclusions DHMMR demonstrates pronounced suppressive effects on the progression of DR after retinal laser photocoagulation,through down-regulating VEGF and upregulating PEDF.
基金Supported by National Natural Science Foundation ( No.30371516) , the Scientific Research Foundation for the ReturnedOverseas Chinese Scholars, State Education Ministry, China ( 2004) , Natural Science Foundation of Shanxi Province ( No.2004C246) and Scientific and Technological Innovation Foundation of Xijing Hospital ( No.XJCX04M003)
文摘Purpose: Choroidal neovascularization (CNV) plays an important role in pathogenesis of age-related macular degeneration (AMD), ocular histoplasmosis syndrome (OHS) and so on. However, mechanisms of CNV formation are not fully understood. The aim of this study is to investigate the correlation between expressions of CD105 and vascular endothelial growth factor (VEGF) in experimental laser-induced CNV in rats. Methods: CNV model was established by 532 nm laser photocoagulation in Brown-Norway rats. The expression of CD105 and VEGF in CNV was observed by immunohistochemistry at 3, 7, 14, 21, 28 and 56 days after laser photocoagulation. The image analysis was performed with the professional software of Image-Pro Plus. Results: Fluorescein angiography showed fluorescein leakage in CNV from days 7 to 56 after photocoagulation. VEGF expression was mainly observed in vascular endothelial cells, ganglion cells, inner nuclear layers and retinal pigment epithelial cells in normal retina and vascular endothelial cells in normal choroid of the rats. On day 3 after photocoagulation, VEGF began to express in laser-induced lesions. VEGF was strongly expressed in CNV after 7 days (P<0.05) and decreased after 14 days (P>0.05). CD105 was initially presented in CNV at 7 days and obviously expressed at 14 days after photocoagulation (P<0.05). Four weeks later, when angiogenesis tended toward inactive status, expression of CD105 was markedly decreased (P>0.05). There was notablely direct correlation between CD105-positive-microvessel density and positively semiquantitative scoring of VEGF in the CNV(r=0.989, P<0.01). Conclusions: There is direct correlation between the expression of CD105 and VEGF in the laser-induced CNV in rat. It suggests that CD105 and VEGF might participate in the new blood vessel formation and promote the growth of CNV.
