Total flavonoids of bugloss limits left ventricular remodeling after myocardial infarction in mice

OBJECTIVE To investigate the effects of total flavonoids of bugloss(TFB) on left ventricular(LV) remodeling after myocardial infarction(MI),LV size and function was compared in mice subjected to left anterior descending coronary artery ligation.METHODS 28 d after MI,the infarcted fraction of the LV and LV mass,systolic and diastolic function were measured.Capillary density and myocyte width in the nonischemic portion of the LV were also determined.RESULTS 28 d after MI,both groups had dilated LVs with decreased fractional shortening and lower ejection fractions.Although the infarcted size of the LV was similar in both groups,LV end-diastolic internal diameter,end-diastolic volume,and mass were lower,but fractional shortening,ejection fraction,and the maximum rate of developed LV pressure(dp/dtmax) were greater in TFB treated mice than in control mice.Impairment of diastolic func.tion,as measured by the time constant of isovolumic relaxation(t) and the maximum rate of LV pres.sure decay(dp/dtmin),was more marked in control mice than in TFB treated mice.Mortality after MI was greater in control mice than in TFB treated mice.In control mice,capillary density and myocyte width in the nonischemic portion of the LV did not differ before and 28 days after MI,whereas in TFB treated mice,capillary density increased and myocyte width declined after MI.CONCLUSION These results suggest that the presence of TFB limits LV dysfunction and remodeling in a murine model of MI in part by decreasing myocyte hypertrophy in the remote myocardium.

基于牛舌草指纹图谱的多指标成分相对定量与鉴别方法

色谱指纹图谱法在中草药产地鉴别或中成药鉴别中发挥着重要作用,然而色谱峰强度和位置易受目标物提取和分离条件的影响,发展更加准确的指纹图谱具有重要意义。该文以色谱峰中某一个常见组分(芦丁)作为内标,分析其他组分的相对含量,以相对含量作为指纹图谱信息,结合化学模式识别可以精确给出产地的相似度。以牛舌草的液相色谱指纹图谱指纹峰为例,以其中芦丁的组分峰作为基准峰,其他指纹峰以芦丁为参照,计算每个指纹峰以芦丁计的含量,建立了牛舌草的指纹图谱。将获得的相对含量指纹图谱采用系统聚类分析和主成分分析进行化学模式识别研究,实现了不同产地牛舌草的区分。该方法建立了牛舌草多指标成分的相对定量与鉴别方法,既节省资源,又具有可操作性,对于其他中药或中成药的质量控制具有一定的借鉴意义。

牛舌草总黄酮抗大鼠心肌缺血再灌注损伤的作用及机制

牛舌草是维吾尔医长期实践中治疗心脑血管疾病的常用药材之一。本研究采用大鼠冠状动脉左前降支结扎30 min,复灌4 h模拟临床心肌缺血再灌注(ischemia/reperfusion,I/R)损伤,于再灌注后腹腔注射给予10、30和50 mg·kg-1牛舌草总黄酮(bugloss total flavonoids,BTF),根据心电图、心功能、心肌梗死指数和血清心肌酶谱结果评价牛舌草总黄酮的心肌保护作用,并从炎症、细胞凋亡以及PI3K/Akt信号通路等探讨其作用机制。实验结果显示,牛舌草总黄酮能够剂量依赖性地降低心肌梗死指数,改善缺血再灌注大鼠的心脏功能,减少心肌酶的漏出,具有明显的心肌保护作用。ELISA结果表明,牛舌草总黄酮能降低心肌炎症因子IL-1β、IL-6、TNF-α的水平,增加Bcl-2,降低Bax,并上调PI3K和Akt的磷酸化水平。牛舌草总黄酮具有明显的抗心肌缺血再灌注损伤作用,其机制可能与上调PI3K/AKT信号通路而抑制细胞凋亡与炎症有关。