Near isogenic lines carrying large-effect QTL （qtl2.1）, which has a consistent influence on grain yield under upland drought stress conditions in a wide range of environments, were evaluated under water stress in th...Near isogenic lines carrying large-effect QTL （qtl2.1）, which has a consistent influence on grain yield under upland drought stress conditions in a wide range of environments, were evaluated under water stress in the fields. The line which gave higher yield under drought was crossed with a local elite line, PMK3, and forwarded to F2：3 generation. Significant variation was found among the F2：3 lines for agronomic traits under water stress in the fields. Low to high broad sense heritability （H） for investigated traits was also found. Water stress indicators such as leaf rolling and leaf drying were negatively correlated with plant height, biomass and grain yield under stress. Bulked segregant analysis （BSA） was performed with the markers in the vicinity of qUl2.1, and RM27933 was found to be segregated perfectly well in individual components of drought resistant and drought susceptible bulks which were bulked based on yield under water stress among F2：3 lines. Hence, this simple and breeder friendly marker, RM27933, may be useful as a potentially valuable candidate marker for the transfer of the QTL qtl12.1 in the regional breeding program. Bioinformatic analysis of the DNA sequence of the qtl12.1 region was also done to identify and analyze positional candidate genes associated with this QTL and to ascertain the putative molecular basis of qUl2.1.展开更多
Background: Urinary incontinence mainly affects women regardless of age and, as it affects their quality of life, influences work, sex life and independence for activities of daily living. The treatment of stress urin...Background: Urinary incontinence mainly affects women regardless of age and, as it affects their quality of life, influences work, sex life and independence for activities of daily living. The treatment of stress urinary incontinence including urethral volume injection therapy can provide an intermediate option over non-surgical and surgical therapies. One of the mechanisms for stress continence depends on the effective coaptation of the urethra during the increase in intra-abdominal pressure. The bulking agents can be injected transurethral or periurethral retrogradely, using direct vision from a cystoscope. Purpose: To evaluate the feasibility and preliminary outcome performance of the bacterial polysaccharide gel used as biological bulking agent applied in female patients with stress urinary incontinence. Methods: A prospective clinical pilot study was performed, in a single institution, including female patients who were admitted to the urologic outpatient clinic with Stress Urinary Incontinence (SUI) without previous treatments and they were selected and underwent bulking agent procedure. The evaluation was performed at the time of enrollment and 6 months after treatment. The primary outcome was Quality of Life (QOL) using the ICIQ-SF Questionnaire. The amount of urine leakage measured by the 1-HOUR PAD-TEST was the second outcome. Results: Fifteen women (with an average age of 53 years) were submitted to the application of bacterial cellulose gel and she was analyzed. Only two patients presented unchanged incontinence. The study considered as primary outcome the improvement or disappearance of symptoms after six months of intervention. Post-intervention Quality of Life (QOL) questionnaire indicated that all of these patients related a better quality of life (62.5%). Through the PAD-test it was possible to observe a decrease in urinary leak of 85% comparing the results pre and post-intervention (BCA—Bacterial Cellulose Application) with p-value equal to 0.000009. Conclusions: The results of this pilot study suggest that the use of biological bulking agent is a promising approach to treat stress urinary incontinence in female patients. Trial registration: Registration number and date of registration should be instated in this section.展开更多
This paper describes a micro thermal shear stress sensor with a cavity underneath, based on vacuum anodic bonding and bulk micromachined technology. A Ti/Pt alloy strip, 2μm×100μm, is deposited on the top of a ...This paper describes a micro thermal shear stress sensor with a cavity underneath, based on vacuum anodic bonding and bulk micromachined technology. A Ti/Pt alloy strip, 2μm×100μm, is deposited on the top of a thin silicon nitride diaphragm and functioned as the thermal sensor element. By using vacuum anodic bonding and bulk-si anisotropic wet etching process instead of the sacrificial-layer technique, a cavity, functioned as the adiabatic vacuum chamber, 200μm×200μm×400μm, is placed between the silicon nitride diaphragm and glass （Corning 7740）. This method totally avoid adhesion problem which is a major issue of the sacrificial-layer technique.展开更多
The use of injectable agents for the treatment of stress urinary incontinence(SUI)is an option for female patients who are unwilling to undergo surgery,or have concurrent conditions or diseases that render surgical tr...The use of injectable agents for the treatment of stress urinary incontinence(SUI)is an option for female patients who are unwilling to undergo surgery,or have concurrent conditions or diseases that render surgical treatment unsuitable.To be effective for SUI,an injectable agent must be nonimmunogenic,hypoallergenic,biocompatible,permanent,nonerosive,nonmigratory and painless.It must also heal with minimal fibrosis,possess a long-term bulking effect,and be easily stored and handled.Glutaraldehyde cross-linked bovine collagen(Contigen),silicone polymers(Macroplastique),Durasphere,calcium hydroxyapatite(Coaptite),polyacrylamide hydrogel(Aquamid,Bulkamid),Permacol,and stem cell therapy have been used as injectable agents.Patients must be informed that treatment with injectable agents is not as effective as surgical treatment,and that such agents might necessitate additional and repeated administrations in order to achieve the desired therapeutic effect.展开更多
粮仓中粮堆在装卸时存在着复杂的应力路径,为了得出复杂应力路径对粮堆模量和临界状态特性的影响规律,以及粮堆应力应变关系模型,该文在侧向应力50~300 k Pa下,进行了常规三轴压缩(conventional triaxial compression,CTC)、等p压缩(con...粮仓中粮堆在装卸时存在着复杂的应力路径,为了得出复杂应力路径对粮堆模量和临界状态特性的影响规律,以及粮堆应力应变关系模型,该文在侧向应力50~300 k Pa下,进行了常规三轴压缩(conventional triaxial compression,CTC)、等p压缩(constant mean normal stress compression,CMS)、三轴主动压缩(reduced triaxial compression,RTC)三轴应力路径试验,分析了应力路径和侧向压力对模量的影响和粮堆临界状态特性;修正岩土体三次曲线模型,建立了适于描述仓内小麦粮堆应力应变的模型,并通过应力路径试验结果和文献试验结果对模型的适用性进行验证。研究结果表明:各应力路径下初始模量、割线模量E50均随着侧向应力呈幂函数增长;CTC、CMS试验的割线模量E50比初始模量发生较大的降低,而RTC试验没有明显降低。在参考压力(大气压力)下,对于初始模量,CTC试验的结果最大,RTC试验的结果最小;对于割线模量E50,CTC试验的结果最小,RTC试验的结果最大。CTC试验的初始模量、割线模量均随着侧向应力增长最慢,而RTC试验的结果均随着侧向应力增长最快。不同应力路径和侧向应力下,试验的破坏点均落于同一临界状态线上,小麦粮堆临界状态应力比为0.976。修正三次曲线模型反映了粮堆强度、峰度系数和峰值应变等特性,并通过8个参数进行计算;通过应力路径试验结果和文献试验结果对该模型进行了验证。研究结果可为粮仓装卸料压力、变形的计算提供更符合实际应力路径条件的参量,建立的修正三次曲线模型可用于粮堆应力和变形的数值模拟,为粮仓的设计提供参考。展开更多
用容重为1.2和1.5 g cm-3的土壤进行盆栽试验,研究了紧实胁迫对土壤剖面各层中呼吸强度及CO2浓度的影响,调查了黄瓜植株及果实品质对土壤紧实胁迫的反应。结果表明:紧实土壤(高容重)土面下CO2浓度及呼吸强度均大于疏松土壤(低容重)。紧...用容重为1.2和1.5 g cm-3的土壤进行盆栽试验,研究了紧实胁迫对土壤剖面各层中呼吸强度及CO2浓度的影响,调查了黄瓜植株及果实品质对土壤紧实胁迫的反应。结果表明:紧实土壤(高容重)土面下CO2浓度及呼吸强度均大于疏松土壤(低容重)。紧实土壤中黄瓜根系伸长生长受到抑制,根系重量显著减小,根冠比(地下部干重/地上部干重)降低;地上部的鲜重及干重也减小,但干物质含量却有一定程度增加;展开的叶片数减小,黄叶数增加,植株衰老提早。果实增大速度受到抑制,糖/酸比大幅度下降,风味变差。展开更多
基金funded by the Generation Challenge Programme Grant in coordination with the Global Partnership Initiative for Plant Breeding Capacity Building and Global Crop Diversity Trust
文摘Near isogenic lines carrying large-effect QTL （qtl2.1）, which has a consistent influence on grain yield under upland drought stress conditions in a wide range of environments, were evaluated under water stress in the fields. The line which gave higher yield under drought was crossed with a local elite line, PMK3, and forwarded to F2：3 generation. Significant variation was found among the F2：3 lines for agronomic traits under water stress in the fields. Low to high broad sense heritability （H） for investigated traits was also found. Water stress indicators such as leaf rolling and leaf drying were negatively correlated with plant height, biomass and grain yield under stress. Bulked segregant analysis （BSA） was performed with the markers in the vicinity of qUl2.1, and RM27933 was found to be segregated perfectly well in individual components of drought resistant and drought susceptible bulks which were bulked based on yield under water stress among F2：3 lines. Hence, this simple and breeder friendly marker, RM27933, may be useful as a potentially valuable candidate marker for the transfer of the QTL qtl12.1 in the regional breeding program. Bioinformatic analysis of the DNA sequence of the qtl12.1 region was also done to identify and analyze positional candidate genes associated with this QTL and to ascertain the putative molecular basis of qUl2.1.
文摘Background: Urinary incontinence mainly affects women regardless of age and, as it affects their quality of life, influences work, sex life and independence for activities of daily living. The treatment of stress urinary incontinence including urethral volume injection therapy can provide an intermediate option over non-surgical and surgical therapies. One of the mechanisms for stress continence depends on the effective coaptation of the urethra during the increase in intra-abdominal pressure. The bulking agents can be injected transurethral or periurethral retrogradely, using direct vision from a cystoscope. Purpose: To evaluate the feasibility and preliminary outcome performance of the bacterial polysaccharide gel used as biological bulking agent applied in female patients with stress urinary incontinence. Methods: A prospective clinical pilot study was performed, in a single institution, including female patients who were admitted to the urologic outpatient clinic with Stress Urinary Incontinence (SUI) without previous treatments and they were selected and underwent bulking agent procedure. The evaluation was performed at the time of enrollment and 6 months after treatment. The primary outcome was Quality of Life (QOL) using the ICIQ-SF Questionnaire. The amount of urine leakage measured by the 1-HOUR PAD-TEST was the second outcome. Results: Fifteen women (with an average age of 53 years) were submitted to the application of bacterial cellulose gel and she was analyzed. Only two patients presented unchanged incontinence. The study considered as primary outcome the improvement or disappearance of symptoms after six months of intervention. Post-intervention Quality of Life (QOL) questionnaire indicated that all of these patients related a better quality of life (62.5%). Through the PAD-test it was possible to observe a decrease in urinary leak of 85% comparing the results pre and post-intervention (BCA—Bacterial Cellulose Application) with p-value equal to 0.000009. Conclusions: The results of this pilot study suggest that the use of biological bulking agent is a promising approach to treat stress urinary incontinence in female patients. Trial registration: Registration number and date of registration should be instated in this section.
基金Project supported by the National Natural Science Foundation of China (Grant No 60576053)Technology Innovation of Chinese Academy of Sciences (Grant No CXJJ-176)
文摘This paper describes a micro thermal shear stress sensor with a cavity underneath, based on vacuum anodic bonding and bulk micromachined technology. A Ti/Pt alloy strip, 2μm×100μm, is deposited on the top of a thin silicon nitride diaphragm and functioned as the thermal sensor element. By using vacuum anodic bonding and bulk-si anisotropic wet etching process instead of the sacrificial-layer technique, a cavity, functioned as the adiabatic vacuum chamber, 200μm×200μm×400μm, is placed between the silicon nitride diaphragm and glass （Corning 7740）. This method totally avoid adhesion problem which is a major issue of the sacrificial-layer technique.
文摘The use of injectable agents for the treatment of stress urinary incontinence(SUI)is an option for female patients who are unwilling to undergo surgery,or have concurrent conditions or diseases that render surgical treatment unsuitable.To be effective for SUI,an injectable agent must be nonimmunogenic,hypoallergenic,biocompatible,permanent,nonerosive,nonmigratory and painless.It must also heal with minimal fibrosis,possess a long-term bulking effect,and be easily stored and handled.Glutaraldehyde cross-linked bovine collagen(Contigen),silicone polymers(Macroplastique),Durasphere,calcium hydroxyapatite(Coaptite),polyacrylamide hydrogel(Aquamid,Bulkamid),Permacol,and stem cell therapy have been used as injectable agents.Patients must be informed that treatment with injectable agents is not as effective as surgical treatment,and that such agents might necessitate additional and repeated administrations in order to achieve the desired therapeutic effect.
文摘粮仓中粮堆在装卸时存在着复杂的应力路径,为了得出复杂应力路径对粮堆模量和临界状态特性的影响规律,以及粮堆应力应变关系模型,该文在侧向应力50~300 k Pa下,进行了常规三轴压缩(conventional triaxial compression,CTC)、等p压缩(constant mean normal stress compression,CMS)、三轴主动压缩(reduced triaxial compression,RTC)三轴应力路径试验,分析了应力路径和侧向压力对模量的影响和粮堆临界状态特性;修正岩土体三次曲线模型,建立了适于描述仓内小麦粮堆应力应变的模型,并通过应力路径试验结果和文献试验结果对模型的适用性进行验证。研究结果表明:各应力路径下初始模量、割线模量E50均随着侧向应力呈幂函数增长;CTC、CMS试验的割线模量E50比初始模量发生较大的降低,而RTC试验没有明显降低。在参考压力(大气压力)下,对于初始模量,CTC试验的结果最大,RTC试验的结果最小;对于割线模量E50,CTC试验的结果最小,RTC试验的结果最大。CTC试验的初始模量、割线模量均随着侧向应力增长最慢,而RTC试验的结果均随着侧向应力增长最快。不同应力路径和侧向应力下,试验的破坏点均落于同一临界状态线上,小麦粮堆临界状态应力比为0.976。修正三次曲线模型反映了粮堆强度、峰度系数和峰值应变等特性,并通过8个参数进行计算;通过应力路径试验结果和文献试验结果对该模型进行了验证。研究结果可为粮仓装卸料压力、变形的计算提供更符合实际应力路径条件的参量,建立的修正三次曲线模型可用于粮堆应力和变形的数值模拟,为粮仓的设计提供参考。
文摘用容重为1.2和1.5 g cm-3的土壤进行盆栽试验,研究了紧实胁迫对土壤剖面各层中呼吸强度及CO2浓度的影响,调查了黄瓜植株及果实品质对土壤紧实胁迫的反应。结果表明:紧实土壤(高容重)土面下CO2浓度及呼吸强度均大于疏松土壤(低容重)。紧实土壤中黄瓜根系伸长生长受到抑制,根系重量显著减小,根冠比(地下部干重/地上部干重)降低;地上部的鲜重及干重也减小,但干物质含量却有一定程度增加;展开的叶片数减小,黄叶数增加,植株衰老提早。果实增大速度受到抑制,糖/酸比大幅度下降,风味变差。
