Transfer of the zp3a gene results in changes in egg adhesiveness and buoyancy in transgenic zebrafish

Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.

Effect of Water Absorption on the Mechanical Property and Failure Mechanism of Hollow Glass Microspheres Composite Epoxy Resin Solid Buoyancy Materials

To study the water absorption of hollow glass microspheres(HGMs)composite epoxy resin solid buoyancy materials in the marine environment and its effect on the mechanical properties,the water absorption was measured by immersing the material in distilled water for 36 days at ambient temperature and fitted to Fick’s second law.The strength of materials before and after water absorption were tested by uniaxial experiments,and the effects of the filling ratio and water absorption on the mechanical properties of the materials were analyzed and explained.Finally,the failure modes and mechanism of the hollow glass microspheres composite material were explicated from the microscopic level by scanning electron microscope(SEM).This research will help solve the problems of solid buoyancy materials in ocean engineering applications.

Cracking and buoyancy effect on hydrocarbon endothermic and heat transfer characteristics in rectangular mini-channel

Although buoyancy and cracking reactions are strongly coupled in the active cooling process, most of the previous studies consider only one of these factors, and their coupling relationship has not been considerably examined. In this work, this coupling relationship was numerically investigated with complete consideration of different cases of heating, and in the view of energy transport and conversion. By comparing with the no-gravity case(NGC), the results indicate that buoyancy has a significant effect on the bottom-heated case(BHC) and side-heated case(SHC), but has little influence on the top-heated case(THC) owing to the different magnitudes of secondary flow. The heat transfer of the BHC and SHC was significantly enhanced by the secondary flow, but their energy conversion was simultaneously impaired.The conversion of the BHC and SHC was approximately half that of the THC and NGC. For all cases, by analyzing the energy transport ways, the cross section can be classified into three regions in the heating direction. Laminar conduction dominates in region Ⅰ, but gradually fails in region Ⅱ, where its role is replaced by other energy transport ways. In region Ⅲ, convection dominates the energy transport for BHC and SHC, whereas turbulence dominates for THC and NGC.

Minimizing Buoyancy Factor of Metallic Pressure-Hull Subjected to Hydrostatic Pressure

To increase the payload,reduce energy consumption,improve work efficiency and therefore must accordingly reduce the total hull weight of the submersible.This paper introduces a design optimization process for the pressurehull of submarines under uniform external hydrostatic pressure using bothfinite element analysis(FEA)and optimization tools.A comprehensive study about the optimum design of the pressure hull,to minimize the weight and increase the volume,to reach minimum buoyancy factor and maximum operating depth minimizing the buoyancy factor(B.F)is taken as an objective function with constraints of plate and frame yielding,general instability and deflection.The optimization process contains many design variables such as pressure-hull plate thickness,unsupported spacing,dimensions of long and ring beams andfinally the elliptical submersible pressure-hull diameters.The optimization process was conducted using ANSYS parametric design language(APDL)and ISIGHT.The Multi-Island Genetic Algorithm(G.A)is considered to conduct the optimization process.Additionally,parametric analysis is done on the pressure hull to examine the effect of different design variables on the pressure-hull design.As a result,the B.F of the proposed optimal model is reduced by an average of 31.78%compared with Reference Model(RM).Maximum von Mises stress is reduced by 27%as well.These results can be helpful for submarine pressure-hull designers.

Seasonal Variability of the Buoyancy Flux along the Northern Coast of the Gulf of Guinea

The buoyancy flux Bo at the air/sea interface is very useful to understand the variability of the stratification of the mixed layer, the oceanic mixing, the phytoplankton dynamics and then the coastal upwelling. The atmospheric reanalysis ERA5 and the oceanic reanalysis ORAP5 data have been used in this study to describe the sea surface Bo and, its influence on the variability of the mixing in the mixed layer and consequently on the coastal upwelling along the northern coast of the Gulf of Guinea. The Bo is negative along the coast and, is characterized by a seasonal variability dominated by the thermal buoyancy flux. This study has also shown that the mixing layer is very shallow along the coast and deeper offshore. The negative value of the Bo increases the stratification of the mixed layer and reduces the mixing. This could explain why the mixed layer is shallow in this region. This work suggests that an increasing trend of the global warming could have dramatic impact in this area by increasing the stratification in the mixed layer and would contribute to reducing the coastal upwelling intensity.

Effect of Thermal Buoyancy on Fluid Flow and Inclusion Motion in Tundish without Flow Control Devices——Part II :Inclusion Motion

Following up the fluid flow simulation in a 60 t tundish, the trajectories of inclusions in the 60 t tundish without flow control are simulated by considering the force balance between the drag force and the inertial buoyancy force. The Stochastic model yields more accurate inclusion motion than the non-Stochastic model due to including the effect of the turbulent fluctuation. The average residence time of inclusions decreases with increasing size. The thermal buoyancy favors inclusions removal especially the small inclusions. Using solute transport like the dye injection in water model and copper addition in the real steel tundish cannot accurately study the motion of the inclusions. In the simulation, more than 68% inclusions bigger than 10μm are removed to the top, and less than 32% enters the mold. The thermal buoyancy has little effect on the fraction of inclusions moved to the top of the inlet zone, and it mainly favors the removal of inclusions smaller than 100μm to the top surface of the outlet zone. For inclusions bigger than 100μm, the effect of thermal buoyancy on their motion can be ignored compared to the inertial buoyancy effect.

Effect of Thermal Buoyancy on Fluid Flow and Inclusion Motion in Tundish without Flow Control Devices——Part Ⅰ: Fluid Flow

The κ-ε two-equation model is used to simulate the fluid flow in the continuous casting tundish coupling with the effect of thermal buoyancy. The natural convection induced by the thermal buoyancy generates an upward flow pattern especially at the outlet zone, and has little effect on the fluid flow in the inlet zone. The maximum viscosity is 700 times larger than the laminar viscosity, which indicates the strong turbulent flow in the tundish. The maximum temperature difference in the whole tundish is 82 K. The temperature near the stopper rod and the short wall is obviously lower than that in the inlet zone. The existence of the stopper rod has a big effect on the fluid flow entering the SEN and the mold. All the characteristics of the tundish geometry should be considered to accurately simulate the fluid flow in the tundish.

Design optimization of composite egg-shaped submersible pressure hull for minimum buoyancy factor

This paper describes a design optimization study of the composite egg-shaped submersible pressure hull employing optimization and finite element analysis(FEA)tools as a first attempt to provide an optimized design of the composite egg-shaped pressure hull for manufacturing or further investigations.A total of 15 optimal designs for the composite egg-shaped pressure hull under hydrostatic pressure are obtained in terms of fibers’angles and the number of layers for 5 lay-up arrangements and 3 unidirectional(UD)composite materials.The optimization process is performed utilizing a genetic algorithm and FEA in ANSYS.The minimization of the buoyancy factor eB:FT is selected as the objective for the optimization under constraints on both material failure and buckling strength.Nonlinear buckling analysis is conducted for one optimal design considering both geometric nonlinearity and imperfections.A sensitivity study is also conducted to further investigate the influence of the design variables on the optimal design of the egg-shaped pressure hull.

Buoyancy leads to high productivity of the Changjiang diluted water:a note

Being the mightiest river emptying into the East China Sea （ECS） and the Pacific Ocean, compounded with the large increase of nitrogen and phosphorus input due to anthropogenic activities, the Changjiang River （Yangtze River） has become a dominating source of these nutrients to the estuary. The high nutrient concentrations notwithstanding, however, outside of the estuary the high biological productivity of the Changjiang diluted water （CDW） are most probably fueled mainly by nutrient-rich subsurface waters originating from the upwelled Kuroshio waters. This is because while the buoyancy of the CDW spreads it out on the ECS continen- tal shelf, the CDW entrains subsurface waters along with the nutrients. Nutrients thus supplied are several times more than those supplied by the Changjiang River.

Buoyancy driven Flow of a Second-Grade Nanofluid flow Taking into Account the Arrhenius Activation Energy and Elastic Deformation:Models and Numerical Results

The buoyancy driven flow of a second-grade nanofluid in the presence of a binary chemical reaction is analyzed in the context of a model based on the balance equations for mass,species concentration,momentum and energy.The elastic properties of the considered fluid are taken into account.The two-dimensional slip flow of such non-Newtonian fluid over a porous flat material which is stretched vertically upwards is considered.The role played by the activation energy is accounted for through an exponent form modified Arrhenius function added to the Buongiorno model for the nanofluid concentration.The effects of thermal radiation are also examined.A similarity transformations is used to turn the problem based on partial differential equations into a system of ordinary differential equations.The resulting system is solved using a fourth order RK and shooting methods.The velocity profile,temperature profile,concentration profile,local skin friction,local Nusselt number and local Sherwood number are reported for several circumstances.The influence of the chemical reaction on the properties of the concentration and momentum boundary layers is critically discussed.

Proposal for Offshore Wind Farm with Triangle-Coupled Movable Parallelogram Buoyancy Structure for the High Seas

We are responsible for providing energy and food resources for our next generation.After more than 20 years of research,the author has confirmed that the VAWT with flip-up mechanism can maintain rotation without the brake,even in strong winds.Experiments have shown that the triangularly coupled movable parallelogram buoyancy structure makes it easy to maintain the verticality of the wind turbine tower even under large waves.Furthermore,as a result of studying the connection between the HAWT(horizontal axis wind turbine)and the movable parallelogram buoyancy structure of the triangular connection,the feasibility was confirmed.The only way for all people in the world to secure energy and food fairly is to look for the remaining areas such as the high seas.The author would like to propose the use of the high seas to combat global hunger,which is likely to be a global problem in the future.

Analysis of Buoyancy Module Auxiliary Installation Technology Based on Numerical Simulation

To reduce the requirement for lifting capacity and decrease the hoist cable force during the descending and laying process of a subsea production system(SPS), a buoyancy module auxiliary installation technology was proposed by loading buoyancy modules on the SPS to reduce the lifting weight. Two models are established, namely, the SPS lowering-down model and the buoyancy module floating-up model. The main study results are the following: 1) When the buoyancy module enters the water under wave condition, the amplitude of tension fluctuation is twice that when SPS enters water; 2) Under current condition, the displacement of SPS becomes three times larger because of the existence of the buoyancy module; 3) After being released, the velocity of the buoyancy module increases to a large speed rapidly and then reaches a balancing speed gradually. The buoyancy module floats up at a balancing speed and rushes out from the water at a pop-up distance; 4) In deep water, the floating-up velocity of the buoyancy module is related to its mass density and shape, and it is not related to water depth; 5) A drag parachute can reduce floating-up velocity and pop-up distance effectively. Good agreement was found between the simulation and experiment results.

Two－Dimensional Model of Hydraulic Fracturing in Geosciences：Effects of Fluid Buoyancy

Hydraulic fracturing occurs in diverse fields of geosciences. We introduce effects of fluid buoyancy into the CGDD (Christianovich-Geertsma-DeKlerk-Daneshy) model of hydranlic fracturing. In the model, a two-dimensional one-sided crack in impermeable rock propagates from a horizontally lying wellbore or fluid reservoir at depth; the crack plane is inclined at a prescribed angle to the horizontal; incompressible and Newtonian fluid less dense than the surrounding rock is injected consecutively from the wellbore or fluid reservoir into the crack at a given injection rate. A solution of the crack propagation is obtained using lubrication theory for turbulent or laminar film flow and linear elastic fracture mechanics. The solution shows the importance of the buoyancy of the fluid in the crack as a driving force or a resisting force of the crack propagation. For example,when the water injection rate into a vertical fracture is 10-2 m2/s and the vertical length of the propagating fracture exceeds 100 m, the fluid buoyancy is important (1) as a driving force if the fracture is formed by the upward propagation of a vertical crack and (2) as a resisting force if it is formed by the downward propagation.

Research and Analysis of the Error of an Unequal Arm Buoyancy Balance

The basic structure and working principle of an unequal-arm buoyancy balance are introduced in this article. Different kinds of error produeed in the unequal-arm buoyancy balance has been researched and analyzed. First the temperature influence of the crossbeam is analyzed, then the measurement error is calculated; second, the error produced by the pivot and the counterweight system is analyzed, then the corresponding error reduction method is proposed. It is shown that the error reduction method can promote the precision of the buoyancy, which is critical to balance.

Nonlinear Thermal Buoyancy on Ferromagnetic Liquid Stream Over a Radiated Elastic Surface with Non Fourier Heat Flux

The current article discusses the heat transfer characteristics of ferromagnetic liquid over an elastic surface with the thermal radiation and non-Fourier heat flux.In most of the existing studies,the heat flux is considered as constant,but whereas we incorporated the non-Fourier flux to get the exact performance of the flow.Also,we excluded the PWT and PHF cases to control the boundary layer of the flow.The governing equations related to our contemplate are changed into non-linear ordinary differential equations(ODE’s)by utilizing appropriate similarity changes,which are at the point enlightened by Runge–Kutta based shooting approach.The equations are broken down concerning boundary conditions and to be explained prescribed wall temperature(PWT)and prescribed heat flux(PHF)cases.The impacts of diverse non-dimensional physical parameters on velocity and temperature profiles are laid out graphically.Also,the assortment of skin friction and local Nusselt number for both PWT and PHF cases for various assessments of non-dimensional parameters have been sorted out.Towards the wrap-up of the examination,we suspect that the friction factor coefficient is higher in the PWT case compared to the PHF case.This result helps to conclude that the flux conditions are useful for cooling applications.

Numerical Analysis of the Influence of Buoyancy Ratio and Dufour Parameter on Thermosolutal Convection in a Square Salt Gradient Solar Pond

Revise the abstract as follows:This work aims to investigate numerically the influence of the buoyancy ratio and the Dufour parameter on thermosolutal convection in a square Salt Gradient Solar Pond(SGSP).The absorption of solar radiation by the saline water,the heat losses and the wind effects via the SGSP free surface are considered.The mathematical model is based on the Navier-Stokes equations used in synergy with the thermal energy equation.These equations are solved using the finite volume method and the Gauss algorithm.Velocity-pressure coupling is implemented through the SIMPLE algorithm.Simulations of the SGSP are performed for three values of buoyancy ratio(N=1,2 and 10),three values of Dufour parameter(Df?0,0.2 and 0.8)and some sample meteorological data(Tangier,Morocco).Results show that the highest dimensionless temperature of the storage zone is found for N=10.In the same zone and for the same value of N,the dimensionless salt concentration decreases very slightly versus time(unlike for N=1 or 2).Moreover,increasing Df from 0 to 0.8 causes a decrease in the dimensionless temperature of the SGSP storage zone and this decrease is more pronounced for N=1 and N=2.

Experimental and numerical study of buoyancy driven flow within a bottom heated vertical concentric cylindrical enclosure

The study of buoyancy driven flow within bottom-heated vertical concentric cylindrical enclosure was important with respect to the processes in chemical and nuclear industries. In this research paper, experimental and numerical study of the axial temperature gradient and the heat transfer mechanism within the enclosure were performed. The numerical simulations were validated by comparing the numerical results with experimentally measured axial temperature. The numerical results of the streamlines within the enclosure depicted the real picture of the buoyancy effects. Eighteen different experiments were performed by using inner cylinder of different materials and outer cylinder of different diameters within the bottom disc temperature range of 353 - 433 K. The CFD simulations were performed to study the buoyancy effects within the enclosure. At the bottom disc with temperature up to 393 K, the streamlines within the inner cylinder were almost the same for both con- figurations being independent of outer cylinder diameter, while at 433 K streamlines within the inner cylinders varied. With larger diameter outer cylinder configuration, the buoyancy effects in the outer annulus were stronger as compared to smaller one.

Surface tension effects on the behaviour of a rising bubble driven by buoyancy force

In the inviscid and incompressible fluid flow regime,surface tension effects on the behaviour of an initially spherical buoyancy-driven bubble rising in an infinite and initially stationary liquid are investigated numerically by a volume of fluid （VOF） method. The ratio of the gas density to the liquid density is 0.001, which is close to the case of an air bubble rising in water. It is found by numerical experiment that there exist four critical Weber numbers We1,~We2,~We3 and We4, which distinguish five different kinds of bubble behaviours. It is also found that when 1≤We2, the bubble will finally reach a steady shape, and in this case after it rises acceleratedly for a moment, it will rise with an almost constant speed, and the lower the Weber number is, the higher the speed is. When We 〉We2, the bubble will not reach a steady shape, and in this case it will not rise with a constant speed. The mechanism of the above phenomena has been analysed theoretically and numerically.

Impacts of a wind stress and a buoyancy flux on the seasonal variation of mixing layer depth in the South China Sea

The seasonal variation of mixing layer depth （MLD） in the ocean is determined by a wind stress and a buoy- ance flux. A South China Sea （SCS） ocean data assimilation system is used to analyze the seasonal cycle of its MLD. It is found that the variability of MLD in the SCS is shallow in summer and deep in winter, as is the case in general. Owing to local atmosphere forcing and ocean dynamics, the seasonal variability shows a regional characteristic in the SCS. In the northern SCS, the MLD is shallow in summer and deep in winter, affected coherently by the wind stress and the buoyance flux. The variation of MLD in the west is close to that in the central SCS, influenced by the advection of strong western boundary currents. The eastern SCS presents an annual cycle, which is deep in summer and shallow in winter, primarily impacted by a heat flux on the air-sea interface. So regional characteristic needs to be cared in the analysis about the MLD of SCS.

Conduction-convection coupled heat transfer around a hollow cylinder under different buoyancy forces

In order to clarify the effect of a buoyancy force on conduction–convection coupled heat transfer in a hollow cylinder, the flow and thermal characteristics were analyzed using an RNG k-ε turbulence model. The Reynolds number was fixed at 1.014 × 10^(6), and the Rayleigh number varied from 1.122 × 10^(10)to 1.088 × 10^(11). Results have shown that, when considering the effect of an opposed buoyancy force, increasing the Rayleigh number has a positive impact on the rate of change and uniformity of the cylinder temperature. The temperature distributions along the axial and circumferential directions are similar for different Rayleigh numbers, but extreme values differ.Along the axial direction, the maximum temperature is obtained at the interface between the variable-diameter part and the constant-diameter part. The maximum dimensionless temperature value decreases to 0.12 when the Rayleigh number increases to 1.088 × 10^(11). Along the circumferential direction, the temperature distribution is affected by the buoyancy force, which results in the temperature of the upper part being higher than that of the lower part. After nondimensionalization of the temperature and time, a correlation was proposed to illustrate the transient heat transfer process quantitatively. The standard deviation of the maximum relative temperature, representing the temperature uniformity, was also calculated. It was found that the difference in the direction of the buoyancy force made a huge difference. Compared with the opposed buoyancy force, the maximum dimensionless temperature is almost two times higher with an assisted buoyancy force. Similarly, the heat transfer coefficient with an assisted buoyancy force is half of that with an opposed buoyancy force. Overall, an assisted buoyancy force plays a negative role in terms of thermal characteristics. The flow field around the hollow cylinder was also illustrated to reveal the mechanism of the buoyancy force on magnitude and direction aspects.