A preliminary study of the relationship between antibiotic administration and changes in bacteriological profile of wound infection in a burn unit

To investigate the relationship between antibiotic administration and the changes in bacteriological profile in a burn unit. Methods: The data of consumption of different kinds of antibiotics, including total antibiotic consumption [expressed as the number of defined daily doses (DDD)] as well as pathogen identification, werecollected in a 8-year period. The constituent ratios of different kinds of antibiotics in total antibiotic consumption toisolation rates of various species of bacteria were calculat...

The Effect and Mechanism of Early Specific Immune RNA for Preventing Infection in Burned Patients

Objective To explore the effect and mechanism of early using specific immune RNA (iRNA) for preventing burn infection. Method 129 patients with serious burn were randomly divided into two groups by double blind method. The control group ( n= 64)was treated with routine method；The therapeutic group(n=65)was given specific iRNA in addition to the routine therapy.The incidence of infection in two groups were investigated；Meantime，with the methods of monoclonal antibody (McAb)APAAP，3 H－TdR incorporation and MTT colorimetery，the various immune functions of patients were determined.Result (1) the incidences of wound infection and bacteriemia after burn in the therapeutic group were markedly lower than those of the control group；(2) the therapeutic group was also superior to the control group in the general condition and time of the wound healing；(3) on the 10th day postburn，the various immune founction tested in the therapeutic group have approximately restored to the normal levels, while those in the control group were still in low levels. The difference between the two groups was statistically significant(P<0.01).Conclusion Specific iRNA，which，when used early after burn，can reduce the incidence of postburn infection ，and improve immune functions of burned patients.

The effect of extensive excision of burn wound with invasive infection on hypermetabolism in burn patients with sepsis

To evaluate the effect of extensive excision of invasive burn wound infection on hypermeta-bolic response in burn patients with sepsis. Methods:Eight patients with major burn, complicated by invasive burn wound infection and sepsis were consecutively admitted to our hospital from September 1997 to October 1998. REEs were monitored by means of Cardiorespiratory Diagnostic System (Medical Graphics Corporation, USA) at patients bedside. Plasma concentration of IL-6、IL-8、TNF-α and LPS were assayed before an...

natural polymer-based bilayer adhesive skin substitute for infected burned wound healing

Thermal wounds are complex and lethal with irregular shapes, risk of infection, slow healing, and large surface area. The mortality rate in patients with infected burns is twice that of non-infected burns. Developing multifunctional skin substitutes to augment the healing rate of infected burns is vital. Herein, we 3D printed a hydrogel scaffold comprising carboxymethyl chitosan (CMCs) and oxidized alginate grafted catechol (O-AlgCat) on a hydrophobic electrospun layer, forming a bilayer skin substitute (BSS). The functional layer (FL) was fabricated by physiochemical crosslinking to ensure favorable biodegradability. The gallium-containing hydrophobic electrospun layer or backing layer (BL) could mimic the epidermis of skin, avoiding fluid penetration and offering antibacterial activity. 3D printed FL contains catechol, gallium, and biologically active platelet rich fibrin (PRF) to adhere to both tissue and BL, show antibacterial activity, encourage angiogenesis, cell growth, and migration. The fabricated bioactive BSS exhibited noticeable adhesive properties (P ≤ 0.05), significant antibacterial activity (P ≤ 0.05), faster clot formation, and the potential to promote proliferation (P ≤ 0.05) and migration (P ≤ 0.05) of L929 cells. Furthermore, the angiogenesis was significantly higher (P ≤ 0.05) when evaluated in vivo and in ovo. The BSS-covered wounds healed faster due to low inflammation and high collagen density. Based on the obtained results, the fabricated bioactive BSS could be an effective treatment for infected burn wounds.