To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB...To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.展开更多
目的:探讨珍骨胶囊对兔膝骨性关节炎软骨下骨c-fos m RNA的影响。方法:动物分组与药物干预:健康6月龄新西兰大白兔64只,按抽签法随机分为2组,即:试验组(32只)、对照组(32只)。术后1周开始分组灌胃治疗:试验组给予珍骨胶囊水溶液10m L/d...目的:探讨珍骨胶囊对兔膝骨性关节炎软骨下骨c-fos m RNA的影响。方法:动物分组与药物干预:健康6月龄新西兰大白兔64只,按抽签法随机分为2组,即:试验组(32只)、对照组(32只)。术后1周开始分组灌胃治疗:试验组给予珍骨胶囊水溶液10m L/d(含珍骨胶囊1.12g)灌胃,对照组给予生理盐水10m L/d灌胃,两组动物均在术后1w、6w、9w、12w分批处死取材。检测指标:采用X线摄片、光镜对软骨下骨进行组织形态学观察,RT-PCR法检测软骨下骨c-fos的表达。结果:珍骨胶囊干预后9w、12w软骨下骨的X线片表现为试验组软骨下骨退变表现明显较对照组轻;光镜观察表现为试验组骨质增生及软骨下骨结构紊乱现象明显较对照组轻;RT-PCR法检测结果显示了试验组的c-fos m RNA的表达表现为各项指标均较低,组间有显著性差异(P<0.05)。结论:珍骨胶囊通过下调c-fos m RNA的表达,降低软骨下骨重塑速率。展开更多
文摘To examine the relationship between expression of the c fosmRNA, ppENKmRNA, iNOSmRNA, iNOS activity and acupuncture analgesia, the acupuncture effect on expression of them in the mouse spinal cord was studied. 20 BALB/C mice were randomly divided into 2 groups: a. the acupuncture group treated with 5 Hz electroacupuncture(EA); b. the control group treated with no EA. The gene expression of c fos, ppENK and iNOS in the spinal cord was detected by using in situ hybridization and RNA dot blot techniques. The iNOS activity was detected by using histochemistry and protein dot blot. The dot blot signals were scanned for statistical analysis. The results showed that the hybridization signals appeared bluish violet color. The c fos signals were localized in the laminae I-II and V-VII, the ppEMK weak signals localized in the superficial lamina and the iNOS signals localized in the cytoplasm of astrocytes of the mouse spinal cord. The pain threshold and all of the dot blot signals were enhanced in the acupuncture group when compared to that in the control group, P<0.05. In the acupuncture group, the intensity of expression of c fosmRNA, ppENKmRNA, iNOSmRNA and iNOS activity was positively correlated with analgesia effect respectively, P<0.05-0.01. The enhanced expression of iNOSmRNA and iNOS suggests that the spinal glia may play a more active role in synaptic function to cause analgesia induced by acupuncture stimulation. Besides, there was a positive correlation in alteration between spinal c fosmRNA and ppENK or c fosmRNA and iNOSmRNA, suggesting that c fos may be involved in ppENKmRNA transcription and c fos and iNOS may have common transactivators.
文摘目的:探讨珍骨胶囊对兔膝骨性关节炎软骨下骨c-fos m RNA的影响。方法:动物分组与药物干预:健康6月龄新西兰大白兔64只,按抽签法随机分为2组,即:试验组(32只)、对照组(32只)。术后1周开始分组灌胃治疗:试验组给予珍骨胶囊水溶液10m L/d(含珍骨胶囊1.12g)灌胃,对照组给予生理盐水10m L/d灌胃,两组动物均在术后1w、6w、9w、12w分批处死取材。检测指标:采用X线摄片、光镜对软骨下骨进行组织形态学观察,RT-PCR法检测软骨下骨c-fos的表达。结果:珍骨胶囊干预后9w、12w软骨下骨的X线片表现为试验组软骨下骨退变表现明显较对照组轻;光镜观察表现为试验组骨质增生及软骨下骨结构紊乱现象明显较对照组轻;RT-PCR法检测结果显示了试验组的c-fos m RNA的表达表现为各项指标均较低,组间有显著性差异(P<0.05)。结论:珍骨胶囊通过下调c-fos m RNA的表达,降低软骨下骨重塑速率。
