西北牧区牧民自制酸奶中c9,t11-CLA含量的研究

共轭亚油酸(CLA)是亚油酸的一组几何和位置异构体,具有多种生理功能。反刍动物肉、乳制品是c9,t11-CLA丰富的天然来源。本研究用气相色谱法测定青海、甘南、天祝和肃北4个不同牧区牧民自制酸奶中c9,t11-CLA的含量,并与市售酸奶和牦牛乳中的含量进行对比。结果表明:采用该方法使牧区酸奶中c9,t11-CLA得到很好的分离,测得青海、甘南、天祝和肃北牧区酸奶中c9,t11-CLA的含量分别为1.007、0.782、0.987、1.196mg/ml酸奶;牧区自制酸奶中c9,t11-CLA的含量与市售酸奶中的含量差异不显著(p>0.05);但含量极显著低于牦牛乳(p<0.01)。

c9,t11-CLA对人结肠癌细胞(Caco-2)的抑制作用

采用体外细胞培养方法,通过MTT实验,研究了c9,t11-CLA(Conjugated Linoleic acid,CLA)在不同浓度,(0,25,50,100,200μmol/L)、不同作用时间(1,2,3,4 d)下,对人结肠癌细胞Caco-2增殖的影响。结果表明,c9,t11-CLA抑制了Caco-2细胞的增殖,且对Caco-2细胞增殖的抑制作用与浓度和作用时间密切相关。

品种对牛肉中c9，t11-共轭亚油酸含量的影响研究

c9,t11-共轭亚油酸(CLA)具有多种生理功能,主要存在反刍动物的肉及乳中。试验采用气相色谱法以c9,t11-CLA甲酯标准品在色谱图上保留时间定性,外标法定量,测定不同品种对牛肉中c9,t11-CLA含量的影响。结果表明:气相色谱法是测定牛肉中c9,t11-CLA含量的良好方法,分析数值准确度高(相对标准差RSD=1.61%)、重现性好(回收率为93.57%,RSD=1.49%)。试验测得牛肉中c9,t11-CLA含量为荷斯坦奶公牛肉>西门塔尔牛肉>新疆土种牛肉>新疆褐牛肉,分别为0.242,0.224,0.163,0.125 mg/g脂肪,品种间差异不显著(p>0.05)。

EFFECT OF CIS-9, TRANS-11-CONJUGATED LINOLEIC ACID ON CELL CYCLE OF MAMMARY ADENOCARCINOMA CELLS(MCF-7)

Objective: To determine the effect of cis-9, trans-1 1-conjugated linoleic acid on the cell cycle of mammary cancer cells (MCF-7) and the possible mechanism of the inhibitory effect of c9,t11-CLA. Methods: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B1, D1, p16ink4a and p21cip/waf1 of MCF-7 cells at various c9,t11-CLA concentrations (25μM, 50μM, 100μM and 200μM), at 24h and 48h. 96% ethand was used as negative control. Results: The cell growth and DNA synthesis of MCF-7 cells were inhibited by c9,t11-CLA. After treatment with various doses of c9,t11-CLA mentioned above for 8 days, the inhibition frequency was 27.18%, 35.43%, 91.05%, and 92.86%, respectively. Inhibitory effect of c9,t11-CLA on DNA synthesis (except for 25μM, 24h) was demonstrated by significantly less incorporation of 3H-TdR than the negative control (P<0.05 and P<0.01). To further investigate the influence of the cell cycle progression, we found that c9,t11-CLA may arrest the cell cycle of MCF-7 cells. Immunocytochemical staining demonstrated that incubation with different concentration of c9,t11-CLA at various times significantly decreased the expression of PCNA, Cyclin A, B1, D1 in MCF-7 cells compared to the negative control (P<0.01), whereas the expression of p16ink4a and p21cip/waf1, cyclin-dependent kinases inhibitors (CDKI), were increased. Conclusions: The cell growth and proliferation of MCF-7 cells is inhibited by c9,t11-CLA via blocking cell cycle, accompanying reduced expression of cyclin A, B1, D1 and enhanced expression of CDKI (p16ink4a and p21cip/waf1).

共轭亚油酸诱导人乳腺癌细胞(MCF-7)凋亡的作用

目的 为了研究共轭亚油酸单体 (c9,t11 CLA)对人乳腺癌细胞 (MCF 7)凋亡的影响。方法 采用细胞生长曲线 ,凋亡细胞的荧光显微镜和电镜观察、流式细胞光度术的检测以及p5 3蛋白表达的方法 ,观察了用不同浓度c9,t11 CLA(2 5、5 0、10 0和 2 0 0 μmol L)诱导MCF 7细胞凋亡作用。结果 c9,t11 CLA可明显抑制MCF 7细胞生长 ,8天的抑制率分别为 -6 0 %、4 5 2 %、99 0 %和 99 4 %。通过荧光显微镜和电镜技术观察到了发生凋亡的MCF 7细胞 ;用流式细胞光度术也检测到c9,t11 CLA可诱导MCF 7细胞产生凋亡 ,并随着c9,t11 CLA浓度的增加 ,细胞凋亡率逐渐增加 ;而p5 3蛋白表达则随着c9,t11 CLA浓度的增加呈现逐渐降低的趋势。结论 c9,t11 CLA可诱导MCF 7细胞产生凋亡 ,这可能是c9,t11 CLA抑制肿瘤细胞生长的机制之一。

日粮中添加共轭亚油酸钙盐对奶牛产奶性能和乳脂中共轭亚油酸含量的影响

试验选择40头泌乳中期、胎次一致、泌乳量相近的中国荷斯坦奶牛,随机分为5组,基础日粮中分别添加0、20、80、140、200 g/d的CLA(由CLA钙盐提供),预试期10 d,正试期50 d。正试期第1、10、20、30、40、50天测定乳产量、常规乳成分和乳脂中c9,t11-CLA含量。结果表明:在日粮中添加CLA对产奶量、乳蛋白和乳糖含量无显著影响(P>0.05)。随日粮中CLA添加量的增加,乳脂率呈指数关系降低(P<0.05);各试验组较对照组全期平均乳脂分别降低13.1%、19.7%2、0.7%和30.2%。随着CLA添加量的增加,乳脂中c9,t11-CLA的含量呈线性增长(P<0.05);各试验组较对照组乳脂中c9,t11-CLA含量分别提高了87.9%、143.5%、140.4%和276.7%,牛奶中c9,t11-CLA的含量分别提高61.7%、135.8%、116.0%和158.1%。