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Cloning and Sequencing of cSZ1 Gene of Eimeria acervulina Changchun Strain
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作者 REN Ke-yan YUAN Shu-xian LI Lin YAO Xin-hua YUAN Dong-mei 《Animal Husbandry and Feed Science》 CAS 2011年第5期20-22,共3页
[ Objective] To analyze Eimetria acervulina cSZl gene sequence and thus to find a candidate antigen for vaccine development.[ Method] According to the cSZl gene sequence of Eimeria acervulina published in GenBank, a p... [ Objective] To analyze Eimetria acervulina cSZl gene sequence and thus to find a candidate antigen for vaccine development.[ Method] According to the cSZl gene sequence of Eimeria acervulina published in GenBank, a pair of specific primers was designed. Then, the cSZl gene of Eirneda acervulina Changchun strain was amplified by reverse-transcription polymerase chain reaction (RT-PCR) and sequenced. [ Result] Compared with the published cSZ1 gene sequence, five nucleotide mutation sites appeared in the cSZ1 gene of Eirneda acervulina Changchun strain. They had a nucleotide sequence similarity of 99.47%, and their encoding region had a nucleotide sequence similarity of 99.61%. Among these mutation sites, two appeared in cSZl gene ORF, and A185G mutation led to substitution of valine by isoleucine. [Conclusion] Protein encoded by cSZ1 gene can be used as a candidate antigen for vaccine development. 展开更多
关键词 Eimeria acervulina cszl gene CLONING SEQUENCE
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