500 grams pork with skin75 grams dried mustard cabbage10 grams Shaoxing wine50 grams sugar100 grams soy sauce2 grams salt5 grams spring onions5 grams ginger500 grams clear stockClean the pork, soak the dried mustard c...500 grams pork with skin75 grams dried mustard cabbage10 grams Shaoxing wine50 grams sugar100 grams soy sauce2 grams salt5 grams spring onions5 grams ginger500 grams clear stockClean the pork, soak the dried mustard cabbage until soft, and clean and slice the spring onion and ginger.Braise the pork in a wok,展开更多
Plant structures and chemicals, which are developed from the shoot apical meristem(SAM), form the main barriers to insect feeding. A plant chimera containing cells of different genetic origins in the SAM will be mor...Plant structures and chemicals, which are developed from the shoot apical meristem(SAM), form the main barriers to insect feeding. A plant chimera containing cells of different genetic origins in the SAM will be morphologically and chemically different compared with the parents and thus may result in differential resistance to herbivores. In this study, we explore if particular elements of plant resistance are localized in one of the layers of SAM; the replacement of one cell layer in a chimera may be linked to change of a single resistance trait to herbivores. The morphology and glucosinolate profiles of two periclinal chimeras(labeled as TTC and TCC, respectively) and grafted parents tuber mustard(labeled as TTT) and red cabbage(labeled as CCC) were compared and the performance of whitefly(Bemisia tabaci) in host selection, oviposition preference and development were assessed under controlled conditions. Both chimeras possessed leaf trichomes as parent tuber mustard TTT, however, TTC had significantly more trichomes than TCC and parent TTT. Leaf wax content of both chimeras was intermediate between the two parents. Five aliphatic and two indole glucosinolates were detected in both chimeras, whereas three aliphatic glucosinolates(3-methyl-sulfinylpropyl, 4-methyl-sulfinylbutyl and 2-hydroxy-3-butenyl) were not detected in tuber mustard, and one aliphatic glucosinolate(3-butenyl) was not detected in red cabbage. Unexpectedly for a chimera, the quantities of two aliphatic glucosinolates(3-methyl-sulfinylpropyl and 4-methyl-sulfinylbutyl) in both TTC and TCC were 3-to 5-fold higher than parents. In olfactory preference assays, B. tabaci showed preference to CCC, followed by TCC, TTC and TTT, and number of eggs laid showed the same pattern: CCC〉TCC〉TTC〉TTT. Interestingly, more whiteflies landed on TTT plants than the other three types in a free choice experiment and the developmental duration from egg to adult was the shortest on TTT and increased in the order TTT展开更多
文摘500 grams pork with skin75 grams dried mustard cabbage10 grams Shaoxing wine50 grams sugar100 grams soy sauce2 grams salt5 grams spring onions5 grams ginger500 grams clear stockClean the pork, soak the dried mustard cabbage until soft, and clean and slice the spring onion and ginger.Braise the pork in a wok,
基金financially supported by the Special Fund for Agro-scientific Research in the Public Interest of China(201203036)the Key Science and Technology Innovation team of the Zhejiang Province,China(2013TD05)the Key Science and Technology Program of Zhejiang Province,China(2015C32048)
文摘Plant structures and chemicals, which are developed from the shoot apical meristem(SAM), form the main barriers to insect feeding. A plant chimera containing cells of different genetic origins in the SAM will be morphologically and chemically different compared with the parents and thus may result in differential resistance to herbivores. In this study, we explore if particular elements of plant resistance are localized in one of the layers of SAM; the replacement of one cell layer in a chimera may be linked to change of a single resistance trait to herbivores. The morphology and glucosinolate profiles of two periclinal chimeras(labeled as TTC and TCC, respectively) and grafted parents tuber mustard(labeled as TTT) and red cabbage(labeled as CCC) were compared and the performance of whitefly(Bemisia tabaci) in host selection, oviposition preference and development were assessed under controlled conditions. Both chimeras possessed leaf trichomes as parent tuber mustard TTT, however, TTC had significantly more trichomes than TCC and parent TTT. Leaf wax content of both chimeras was intermediate between the two parents. Five aliphatic and two indole glucosinolates were detected in both chimeras, whereas three aliphatic glucosinolates(3-methyl-sulfinylpropyl, 4-methyl-sulfinylbutyl and 2-hydroxy-3-butenyl) were not detected in tuber mustard, and one aliphatic glucosinolate(3-butenyl) was not detected in red cabbage. Unexpectedly for a chimera, the quantities of two aliphatic glucosinolates(3-methyl-sulfinylpropyl and 4-methyl-sulfinylbutyl) in both TTC and TCC were 3-to 5-fold higher than parents. In olfactory preference assays, B. tabaci showed preference to CCC, followed by TCC, TTC and TTT, and number of eggs laid showed the same pattern: CCC〉TCC〉TTC〉TTT. Interestingly, more whiteflies landed on TTT plants than the other three types in a free choice experiment and the developmental duration from egg to adult was the shortest on TTT and increased in the order TTT