Objective To investigate the effect of oleanolic acid (OA) on apoptosis,correlation between apoptosis and intracellular calcium,and its mechanism in human lung adenocarcinoma cell line A549. Methods Human lung adenoca...Objective To investigate the effect of oleanolic acid (OA) on apoptosis,correlation between apoptosis and intracellular calcium,and its mechanism in human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma A549 cells were incubated in vitro and assigned with OA concentrations of 0,10,20 and 40μg/mL. The apoptosis status of A549 cell line was detected with Annexin V-FITC/PI by flow cytometry (FCM); fluorescence intensity (FI) of A549 cells was assessed and the level of intracellular calcium was calculated at 24 hour of OA intervention. The relation between apoptosis and calcium FI was illustrated by curve fitting. Results FCM showed that 10,20 and 40μg/mL of OA could induce A549 cell apoptosis,which followed a concentration-effect pattern; 24-hour intervention with 20μg/mL and 40μg/mL OA showed increased A549 cell apoptosis,and was significantly different from that with 0μg/mL OA (P<0.01). The FI of intracellular calcium concentration in 10,20 and 40μg/mL OA groups was significantly higher than that in 0μg/mL group after 24 hours’ intervention,and the FI showed a trend of increase with increased OA concentration (P<0.01). Curve fitting showed a significant correlation between apoptosis rate and intracellular calcium concentration in A549 cells (r=0.981,P<0.01). Regression equation was Y=0.508X-1.627. Conclusion OA plays a role in inducing apoptosis of human lung adenocarcinoma cells in a concentration-dependent manner. The OA-induced apoptosis is responsible for intracellular calcium overload of the tumor.展开更多
The aim of this study was to clarify the response of sympathetic activity to antihypertensive drugs using a mental stress test in hypertensive patients and to determine the effects of antihypertensive drugs on the sym...The aim of this study was to clarify the response of sympathetic activity to antihypertensive drugs using a mental stress test in hypertensive patients and to determine the effects of antihypertensive drugs on the sympathetic activitymediated hemodynamic response to mental stress. Hypertensive patients were divided into three groups according to the type of drug(s) being taken: a calcium antagonist group, an angiotensin II receptor blocker group, and a combination therapy group of calcium antagonists and angiotensin II receptor blockers. The Stroop color-word conflict test was applied as a mental stress test and hemodynamic responses to mental stress were measured, including blood pressure, pulse rate, and skin blood flow. Elevation of blood pressure by mental stress was suppressed in the combination therapy group compared with the calcium antagonist group. Reduction of skin blood flow by mental stress was suppressed in both the angiotensin II blocker group and the combination therapy group compared with the calcium antagonist group. In conclusion, skin blood flow can be a useful tool to evaluate sympathetic activity and combination therapy with calcium antagonists and angiotensin II receptor blockers were the most useful therapy for suppressing the hemodynamic response to mental stress.展开更多
The synergetic effect of calcium carbonate (CC)-fly ash (FA) hybrid filler particles on the mechanical and physical properties of low density polyethylene (LDPE) has been investigated. Low density polyethylene is fill...The synergetic effect of calcium carbonate (CC)-fly ash (FA) hybrid filler particles on the mechanical and physical properties of low density polyethylene (LDPE) has been investigated. Low density polyethylene is filled with varying weight percentages of FA and CC using melt casting. Composites are characterized for mechanical, thermal, microstructural and physical properties. Results show that the flexural strength increases with increases in FA content of the hybrid filler. It is evident from the study that to achieve optimum density a certain combination of both fillers need to be used. The optimum combination of CC and FA for a higher density (1.78 g/cm3) is found to be at 20 wt% FA and 30 wt% CC. An increase of 7.27% in micro-hardness over virgin polyethylene is obtained in composites with 10 wt% FA and 40 wt% CC. The presence of higher amount of CC is seen to be detrimental to the crystallinity of composites. X-ray, FTIR and DSC results show that composite with 45 wt% CC and 5 wt% FA exhibits a typical triclinic polyethylene structure indicating that the composite is amorphous in nature. There was the synergy between FA and CC fillers on flexural strength and crystallinity of composite. However, the fillers show the antagonistic effect on energy at peak and micro-hardness.展开更多
【目的】为提高螺旋输送机的输送效率,降低输送机的功耗与磨损,探究在不同进料速率、螺旋轴转速与几何体摩擦系数下,超细碳酸钙在水平变径变距螺旋输送机内的颗粒流动状态、出口质量流量、输送机功耗与磨损分布。【方法】使用计算流体...【目的】为提高螺旋输送机的输送效率,降低输送机的功耗与磨损,探究在不同进料速率、螺旋轴转速与几何体摩擦系数下,超细碳酸钙在水平变径变距螺旋输送机内的颗粒流动状态、出口质量流量、输送机功耗与磨损分布。【方法】使用计算流体动力学(computational fluid dynamics,CFD)与离散单元法(discrete element method,DEM)双向耦合数值模拟的方法,对螺旋输送机在不同转速下的质量流率进行分析对比,验证数值模型的正确性。【结果】摩擦系数对颗粒的运动有较大影响,颗粒流的轴向速度峰值和质量流率峰值随着摩擦系数的增加先增大再减小;随着下料速度和摩擦系数的增大,输送机功率明显增大,且摩擦系数在高进料速度与低转速的情况下对功耗的影响相对于低进料速度和高转速更加明显;磨损较严重的区域集中在下料口处的螺旋轴与螺旋叶片的边缘处。【结论】简单增大或减小摩擦系数并不能提高颗粒的轴向速度和质量流量,而是存在一个局部最优参数组合;适当地提高转速能够减小颗粒密实度与颗粒停留时间,从而减小输送机的功耗与几何体磨损。展开更多
文摘Objective To investigate the effect of oleanolic acid (OA) on apoptosis,correlation between apoptosis and intracellular calcium,and its mechanism in human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma A549 cells were incubated in vitro and assigned with OA concentrations of 0,10,20 and 40μg/mL. The apoptosis status of A549 cell line was detected with Annexin V-FITC/PI by flow cytometry (FCM); fluorescence intensity (FI) of A549 cells was assessed and the level of intracellular calcium was calculated at 24 hour of OA intervention. The relation between apoptosis and calcium FI was illustrated by curve fitting. Results FCM showed that 10,20 and 40μg/mL of OA could induce A549 cell apoptosis,which followed a concentration-effect pattern; 24-hour intervention with 20μg/mL and 40μg/mL OA showed increased A549 cell apoptosis,and was significantly different from that with 0μg/mL OA (P<0.01). The FI of intracellular calcium concentration in 10,20 and 40μg/mL OA groups was significantly higher than that in 0μg/mL group after 24 hours’ intervention,and the FI showed a trend of increase with increased OA concentration (P<0.01). Curve fitting showed a significant correlation between apoptosis rate and intracellular calcium concentration in A549 cells (r=0.981,P<0.01). Regression equation was Y=0.508X-1.627. Conclusion OA plays a role in inducing apoptosis of human lung adenocarcinoma cells in a concentration-dependent manner. The OA-induced apoptosis is responsible for intracellular calcium overload of the tumor.
文摘The aim of this study was to clarify the response of sympathetic activity to antihypertensive drugs using a mental stress test in hypertensive patients and to determine the effects of antihypertensive drugs on the sympathetic activitymediated hemodynamic response to mental stress. Hypertensive patients were divided into three groups according to the type of drug(s) being taken: a calcium antagonist group, an angiotensin II receptor blocker group, and a combination therapy group of calcium antagonists and angiotensin II receptor blockers. The Stroop color-word conflict test was applied as a mental stress test and hemodynamic responses to mental stress were measured, including blood pressure, pulse rate, and skin blood flow. Elevation of blood pressure by mental stress was suppressed in the combination therapy group compared with the calcium antagonist group. Reduction of skin blood flow by mental stress was suppressed in both the angiotensin II blocker group and the combination therapy group compared with the calcium antagonist group. In conclusion, skin blood flow can be a useful tool to evaluate sympathetic activity and combination therapy with calcium antagonists and angiotensin II receptor blockers were the most useful therapy for suppressing the hemodynamic response to mental stress.
文摘The synergetic effect of calcium carbonate (CC)-fly ash (FA) hybrid filler particles on the mechanical and physical properties of low density polyethylene (LDPE) has been investigated. Low density polyethylene is filled with varying weight percentages of FA and CC using melt casting. Composites are characterized for mechanical, thermal, microstructural and physical properties. Results show that the flexural strength increases with increases in FA content of the hybrid filler. It is evident from the study that to achieve optimum density a certain combination of both fillers need to be used. The optimum combination of CC and FA for a higher density (1.78 g/cm3) is found to be at 20 wt% FA and 30 wt% CC. An increase of 7.27% in micro-hardness over virgin polyethylene is obtained in composites with 10 wt% FA and 40 wt% CC. The presence of higher amount of CC is seen to be detrimental to the crystallinity of composites. X-ray, FTIR and DSC results show that composite with 45 wt% CC and 5 wt% FA exhibits a typical triclinic polyethylene structure indicating that the composite is amorphous in nature. There was the synergy between FA and CC fillers on flexural strength and crystallinity of composite. However, the fillers show the antagonistic effect on energy at peak and micro-hardness.
文摘【目的】为提高螺旋输送机的输送效率,降低输送机的功耗与磨损,探究在不同进料速率、螺旋轴转速与几何体摩擦系数下,超细碳酸钙在水平变径变距螺旋输送机内的颗粒流动状态、出口质量流量、输送机功耗与磨损分布。【方法】使用计算流体动力学(computational fluid dynamics,CFD)与离散单元法(discrete element method,DEM)双向耦合数值模拟的方法,对螺旋输送机在不同转速下的质量流率进行分析对比,验证数值模型的正确性。【结果】摩擦系数对颗粒的运动有较大影响,颗粒流的轴向速度峰值和质量流率峰值随着摩擦系数的增加先增大再减小;随着下料速度和摩擦系数的增大,输送机功率明显增大,且摩擦系数在高进料速度与低转速的情况下对功耗的影响相对于低进料速度和高转速更加明显;磨损较严重的区域集中在下料口处的螺旋轴与螺旋叶片的边缘处。【结论】简单增大或减小摩擦系数并不能提高颗粒的轴向速度和质量流量,而是存在一个局部最优参数组合;适当地提高转速能够减小颗粒密实度与颗粒停留时间,从而减小输送机的功耗与几何体磨损。
