This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the param...This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.展开更多
Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor a...Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor antibodies 1, 4, and 9, and react relatively weakly with factor antibody 5. We have investigated the structural characteristic of cell wall mannan of this strain using a non-degradable method, nuclear magnetic resonance analysis. The results obtained from NMR analysis, the two-dimensional homonuclear Hartmann-Hahn, indicated that C. zeylanoides mannan contains α-1, 3-linked and β-1,2-linked oligomannosyl side chains corresponding to C. guilliermondii specific antigenic factor 9. In addition, it was found that the mannan contains β-1,2-linked oligomannosyl side chains attached to phosphate groups corresponding to anitigenic factor 5, which were not found in C. guilliermondii mannan.展开更多
目的总结4例中枢神经系统念珠菌感染(Central nervous system candidiasis,CNSC)患者的临床表现、实验室检验检查、治疗方案和随访情况,为此类患者的治疗提供参考。方法回顾4例CNSC患者的治疗过程,结合文献分析该类患者的治疗特点。结果...目的总结4例中枢神经系统念珠菌感染(Central nervous system candidiasis,CNSC)患者的临床表现、实验室检验检查、治疗方案和随访情况,为此类患者的治疗提供参考。方法回顾4例CNSC患者的治疗过程,结合文献分析该类患者的治疗特点。结果①4例患者脑脊液培养结果分别为季也蒙念珠菌、白色念珠菌、热带假丝酵母菌、白色念珠菌。②4例患者实验室检验结果脑脊液生化葡萄糖含量降低,蛋白质含量正常或者偏高,脑脊液常规白细胞计数略高,1,3-β-D-葡聚糖正常或偏高。③治疗方案中,2例患者使用伏立康唑,1例患者首选伏立康唑治疗,由于精神系统不良反应停药,另1例为氟康唑治疗无效换用伏立康唑治疗,治愈后随访未复发。2例首选氟康唑治疗患者1例治愈,另1例治疗无效。结论国内文献关于成人CNSC治疗案例报道较少,本文通过对4例患者进行病例回顾和文献分析,为CNSC的临床治疗提供参考。展开更多
文摘This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.
文摘Antigenicity of pathogenic yeast Candida zeylanoides NBRC 10326 was found to have similar to that of Candida guilliermondii, but it was slightly different, i.e. NBRC 10326 strain cells react strongly with the factor antibodies 1, 4, and 9, and react relatively weakly with factor antibody 5. We have investigated the structural characteristic of cell wall mannan of this strain using a non-degradable method, nuclear magnetic resonance analysis. The results obtained from NMR analysis, the two-dimensional homonuclear Hartmann-Hahn, indicated that C. zeylanoides mannan contains α-1, 3-linked and β-1,2-linked oligomannosyl side chains corresponding to C. guilliermondii specific antigenic factor 9. In addition, it was found that the mannan contains β-1,2-linked oligomannosyl side chains attached to phosphate groups corresponding to anitigenic factor 5, which were not found in C. guilliermondii mannan.
文摘目的总结4例中枢神经系统念珠菌感染(Central nervous system candidiasis,CNSC)患者的临床表现、实验室检验检查、治疗方案和随访情况,为此类患者的治疗提供参考。方法回顾4例CNSC患者的治疗过程,结合文献分析该类患者的治疗特点。结果①4例患者脑脊液培养结果分别为季也蒙念珠菌、白色念珠菌、热带假丝酵母菌、白色念珠菌。②4例患者实验室检验结果脑脊液生化葡萄糖含量降低,蛋白质含量正常或者偏高,脑脊液常规白细胞计数略高,1,3-β-D-葡聚糖正常或偏高。③治疗方案中,2例患者使用伏立康唑,1例患者首选伏立康唑治疗,由于精神系统不良反应停药,另1例为氟康唑治疗无效换用伏立康唑治疗,治愈后随访未复发。2例首选氟康唑治疗患者1例治愈,另1例治疗无效。结论国内文献关于成人CNSC治疗案例报道较少,本文通过对4例患者进行病例回顾和文献分析,为CNSC的临床治疗提供参考。
