Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(...Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six environments.Seven major QTLs,QGl.cib-2D,QGw.cib-2D,QGw.cib-3B,QGw.cib-4B.1,QGlw.cib-2D.1,QTgw.cib-2D.1 and QTgw.cib-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic variance.Significant interactions were detected between the two major TGW QTLs and three major GW loci.In addition,QTgw.cib-3B.1 and QGw.cib-3B were co-located,and the improved TGW at this locus was contributed by GW.Unlike other loci,QTgw.cib-3B.1/QGw.cib-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that QTgw.cib-3B.1/QGw.cib-3B is likely a novel locus.Six haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and cultivars.According to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of QTgw.cib-3B.1/QGw.cib-3B was predicted.Collectively,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.展开更多
The non-local means (NLM) denoising method replaces each pixel by the weighted average of pixels with the sur-rounding neighborhoods. In this paper we employ a cosine weighting function instead of the original exponen...The non-local means (NLM) denoising method replaces each pixel by the weighted average of pixels with the sur-rounding neighborhoods. In this paper we employ a cosine weighting function instead of the original exponential func-tion to improve the efficiency of the NLM denoising method. The cosine function outperforms in the high level noise more than low level noise. To increase the performance more in the low level noise we calculate the neighborhood si-milarity weights in a lower-dimensional subspace using singular value decomposition (SVD). Experimental compari-sons between the proposed modifications against the original NLM algorithm demonstrate its superior denoising per-formance in terms of peak signal to noise ratio (PSNR) and histogram, using various test images corrupted by additive white Gaussian noise (AWGN).展开更多
In order to solve instability problem of calculation precision resulting from the selection of each target weight in evaluating weapon systems, a weighted sum based method is proposed. Specif- ically, the subjective w...In order to solve instability problem of calculation precision resulting from the selection of each target weight in evaluating weapon systems, a weighted sum based method is proposed. Specif- ically, the subjective weights depending on experts＇ experience are substituted by the optimal weights. The optimal weights are acquired through constructing a mathematical programming model based on subjective weights and objective weights. The method of solving subjective weights is the same as before, and the objective weights were solved by means of grey theory. The case analysis shows that the method of improved weighted sum can improve the evaluation precision up to more than 5% , and minimize the instability of calculation precision resulting from only using subjective weights. The method that the optimal weights substituted the subjective weights is brought forward in improving evaluation precision for the first time. The ideas of the optimal weights and the pro- posed method are described and analyzed.展开更多
Thousand-kernel weight(TKW)is a measure of grain weight,a target of wheat breeding.The object of this study was to fine-map a stable quantitative trait loci(QTL)for TKW and identify its candidate gene in a recombinant...Thousand-kernel weight(TKW)is a measure of grain weight,a target of wheat breeding.The object of this study was to fine-map a stable quantitative trait loci(QTL)for TKW and identify its candidate gene in a recombinant inbred line(RIL)population derived from the cross of Kenong 9204(KN9204)and Jing411(J411).On a high-density genetic linkage map,24,26 and 25 QTL were associated with TKW,kernel length(KL),and kernel width(KW),respectively.A major and stable QTL,QTkw-2D,was mapped to an8.3 cM interval on chromosome arm 2DL.By saturation of polymorphic markers in its target region,QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line(F6:7).This interval was further narrowed to 2.52 Mb using QTkw-2D near-isogenic lines(NILs).NILs~(KN9204)had higher fresh and dry weights than NILsJ411at various grain-filling stages.The TKW and KW of NILs~(KN9204)were much higher than those of NILsJ411in field trials.By comparison of both DNA sequence and expression between KN9204 and J411,TraesCS2D02G460300.1(TraesKN2D01HG49350)was assigned as a candidate gene for QTkw-2D.This was confirmed by RNA sequencing(RNA-seq)of QTkw-2D NILs.These results provide the basis of map-based cloning of QTkw-2D,and DNA markers linked to the candidate gene may be used in marker-assisted selection.展开更多
目的基于热力耦合机理,应用模型数值仿真和多参数化的评价机制,实施方案寻优,从而提升齿轮坯闭式热锻工艺的设计效率和成形质量。方法基于DEFORM仿真软件对某工程齿轮坯锻件的3种闭式热模锻成形工艺方案进行CAE仿真分析,研究其金属塑性...目的基于热力耦合机理,应用模型数值仿真和多参数化的评价机制,实施方案寻优,从而提升齿轮坯闭式热锻工艺的设计效率和成形质量。方法基于DEFORM仿真软件对某工程齿轮坯锻件的3种闭式热模锻成形工艺方案进行CAE仿真分析,研究其金属塑性流动规律,并采用TOPSIS(Technique for Order Preferences by Similarity to Ideal Solution)和CRITIC(Criteria Importance Though Intercrieria Correlation)相结合的方法,构建以应变、应力、载荷、充填性、磨损等8个评价指标的评估模型及权值计算方法,进行工艺方案评估。结果通过仿真可以发现不同的预锻方案,金属塑性流动方式不同,成形结果不同,其中方案1未填满,成形有毛刺,受载不均匀,模具损伤值较大;方案2受载均匀,填充饱满,模具磨损深度大,等效应力大;方案3成形质量较好,但受载不均匀,且同样模具磨损深度大,等效应力大;综合考虑锻件成形质量和模具使用寿命,通过指标同向标准化处理、权重计算,得到方案1、2、3贴近程度分别为0.446、0.511、0.556,方案3值最大,结果最优。对方案3进行工艺试验,获得的齿轮坯充填饱满、无折叠缺陷。结论通过数值模拟与评估模型相结合的方法,可以精确、高效地评估闭式热锻造工艺方案的优劣,提高设计效率与成形质量。展开更多
基金supported by the Major Program of National Agricultural Science and Technology of China(NK20220607)the West Light Foundation of the Chinese Academy of Sciences(2022XBZG_XBQNXZ_A_001)the Sichuan Science and Technology Program,China(2022ZDZX0014)。
文摘Grain weight is one of the key components of wheat(Triticum aestivum L.)yield.Genetic manipulation of grain weight is an efficient approach for improving yield potential in breeding programs.A recombinant inbred line(RIL)population derived from a cross between W7268 and Chuanyu 12(CY12)was employed to detect quantitative trait loci(QTLs)for thousand-grain weight(TGW),grain length(GL),grain width(GW),and the ratio of grain length to width(GLW)in six environments.Seven major QTLs,QGl.cib-2D,QGw.cib-2D,QGw.cib-3B,QGw.cib-4B.1,QGlw.cib-2D.1,QTgw.cib-2D.1 and QTgw.cib-3B.1,were consistently identified in at least four environments and the best linear unbiased estimation(BLUE)datasets,and they explained 2.61 to 34.85%of the phenotypic variance.Significant interactions were detected between the two major TGW QTLs and three major GW loci.In addition,QTgw.cib-3B.1 and QGw.cib-3B were co-located,and the improved TGW at this locus was contributed by GW.Unlike other loci,QTgw.cib-3B.1/QGw.cib-3B had no effect on grain number per spike(GNS).They were further validated in advanced lines using Kompetitive Allele Specific PCR(KASP)markers,and a comparison analysis indicated that QTgw.cib-3B.1/QGw.cib-3B is likely a novel locus.Six haplotypes were identified in the region of this QTL and their distribution frequencies varied between the landraces and cultivars.According to gene annotation,spatial expression patterns,ortholog analysis and sequence variation,the candidate gene of QTgw.cib-3B.1/QGw.cib-3B was predicted.Collectively,the major QTLs and KASP markers reported here provide valuable information for elucidating the genetic architecture of grain weight and for molecular marker-assisted breeding in grain yield improvement.
文摘The non-local means (NLM) denoising method replaces each pixel by the weighted average of pixels with the sur-rounding neighborhoods. In this paper we employ a cosine weighting function instead of the original exponential func-tion to improve the efficiency of the NLM denoising method. The cosine function outperforms in the high level noise more than low level noise. To increase the performance more in the low level noise we calculate the neighborhood si-milarity weights in a lower-dimensional subspace using singular value decomposition (SVD). Experimental compari-sons between the proposed modifications against the original NLM algorithm demonstrate its superior denoising per-formance in terms of peak signal to noise ratio (PSNR) and histogram, using various test images corrupted by additive white Gaussian noise (AWGN).
基金Supported by the Natonal Natural Science Foundation of China(5145781)
文摘In order to solve instability problem of calculation precision resulting from the selection of each target weight in evaluating weapon systems, a weighted sum based method is proposed. Specif- ically, the subjective weights depending on experts＇ experience are substituted by the optimal weights. The optimal weights are acquired through constructing a mathematical programming model based on subjective weights and objective weights. The method of solving subjective weights is the same as before, and the objective weights were solved by means of grey theory. The case analysis shows that the method of improved weighted sum can improve the evaluation precision up to more than 5% , and minimize the instability of calculation precision resulting from only using subjective weights. The method that the optimal weights substituted the subjective weights is brought forward in improving evaluation precision for the first time. The ideas of the optimal weights and the pro- posed method are described and analyzed.
基金jointly supported by the National Natural Science Foundation of China(32272056,U22A6009,31671673,and 31871612)Hebei Natural Science Foundation(C2021205013,C2022204202)+1 种基金Talents Program of Hebei Agricultural University in China(YJ2021016)China Agriculture Research System of MOF and MARA(CARS-03)。
文摘Thousand-kernel weight(TKW)is a measure of grain weight,a target of wheat breeding.The object of this study was to fine-map a stable quantitative trait loci(QTL)for TKW and identify its candidate gene in a recombinant inbred line(RIL)population derived from the cross of Kenong 9204(KN9204)and Jing411(J411).On a high-density genetic linkage map,24,26 and 25 QTL were associated with TKW,kernel length(KL),and kernel width(KW),respectively.A major and stable QTL,QTkw-2D,was mapped to an8.3 cM interval on chromosome arm 2DL.By saturation of polymorphic markers in its target region,QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line(F6:7).This interval was further narrowed to 2.52 Mb using QTkw-2D near-isogenic lines(NILs).NILs~(KN9204)had higher fresh and dry weights than NILsJ411at various grain-filling stages.The TKW and KW of NILs~(KN9204)were much higher than those of NILsJ411in field trials.By comparison of both DNA sequence and expression between KN9204 and J411,TraesCS2D02G460300.1(TraesKN2D01HG49350)was assigned as a candidate gene for QTkw-2D.This was confirmed by RNA sequencing(RNA-seq)of QTkw-2D NILs.These results provide the basis of map-based cloning of QTkw-2D,and DNA markers linked to the candidate gene may be used in marker-assisted selection.
文摘目的基于热力耦合机理,应用模型数值仿真和多参数化的评价机制,实施方案寻优,从而提升齿轮坯闭式热锻工艺的设计效率和成形质量。方法基于DEFORM仿真软件对某工程齿轮坯锻件的3种闭式热模锻成形工艺方案进行CAE仿真分析,研究其金属塑性流动规律,并采用TOPSIS(Technique for Order Preferences by Similarity to Ideal Solution)和CRITIC(Criteria Importance Though Intercrieria Correlation)相结合的方法,构建以应变、应力、载荷、充填性、磨损等8个评价指标的评估模型及权值计算方法,进行工艺方案评估。结果通过仿真可以发现不同的预锻方案,金属塑性流动方式不同,成形结果不同,其中方案1未填满,成形有毛刺,受载不均匀,模具损伤值较大;方案2受载均匀,填充饱满,模具磨损深度大,等效应力大;方案3成形质量较好,但受载不均匀,且同样模具磨损深度大,等效应力大;综合考虑锻件成形质量和模具使用寿命,通过指标同向标准化处理、权重计算,得到方案1、2、3贴近程度分别为0.446、0.511、0.556,方案3值最大,结果最优。对方案3进行工艺试验,获得的齿轮坯充填饱满、无折叠缺陷。结论通过数值模拟与评估模型相结合的方法,可以精确、高效地评估闭式热锻造工艺方案的优劣,提高设计效率与成形质量。
