Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of ...Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of Cangfu Daotan pills were searched in the database of traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).GeneCards database and OMIM database were used to search for disease targets of PCOS;the intersection of the target of Cangfu Daotan pill and the disease target of PCOS was drawn by using R to obtain the intersection gene;protein interaction network was constructed through String online database;Cytoscape(3.2.1)was used to construct a‘major component-disease-target’network diagram and analyze the important nodes therein;KEGG and GO enrichment analyses were carried out on the obtained intersection genes through org data packets in R.Results:Network pharmacology analysis showed that 151 active ingredients involved in pathways were screened out,140 of which acted on PCOS,and 105 potential targets were used to exert therapeutic effects.After enrichment,1900 GO biological signals and 143 KEGG signals were obtained,and the first 14 drug targets were screened out.Conclusion:Cangfu Daotan pill may mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation and other signals through regulating key targets of JUN,MAPK3,HSP90AA1,MAPK1,ESR1,NR3C1,APP,MAPK14,MAPK8,VEGFA,EGFR,RB1,AR,IL6,mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation,etc.,and regulate biological processes such as drug reaction,nutrition and lipid metabolism,active oxygen,sugar metabolism,steroid hormone,etc.to achieve the effect of treating polycystic ovary syndrome.展开更多
目的:运用网络药理学方法探讨苍附导痰丸治疗多囊卵巢综合症(polycystic ovary syndrome,PCOS)的作用靶点及分子机制。方法:在中药系统药理学数据库(traditional Chinese medicine systems pharmacology database and analysis platform...目的:运用网络药理学方法探讨苍附导痰丸治疗多囊卵巢综合症(polycystic ovary syndrome,PCOS)的作用靶点及分子机制。方法:在中药系统药理学数据库(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索苍附导痰丸的主要活性成分及对应的作用靶点;利用GeneCards数据库和OMIM数据库搜索多囊卵巢综合症的疾病靶点;运用R数据包图绘制苍附导痰丸作用靶点和多囊卵巢综合症的疾病靶点的交集,得到交集基因;通过String在线数据库构建蛋白互作网络;利用Cytoscape(3.2.1)构建“主要成分-疾病-靶点”网络图,分析其中的重要节点;通过R语言下的org数据包对得到的交集基因进行KEGG和GO富集分析。结果:网络药理学分析表明,共筛选出151个有效活性成分涉及通路,其中作用于PCOS的140个,通过105个潜在靶点发挥疗效。富集得到1900条GO生物信号,143条KEGG信号,筛选出前14个药物靶点。结论:苍附导痰丸可能通过调控JUN、MAPK3、HSP90AA1、MAPK1、ESR1、NR3C1、APP、MAPK14、MAPK8、VEGFA、EGFR、RB1、AR、IL6关键靶点,介导基因转录调节、脂质代谢、炎性微环境、胰岛素抵抗、性激素轴、动脉硬化、白介素免疫调节等信号,调控药物反应、营养与脂质代谢、活性氧、糖代谢、类固醇激素等生物进程调控达到治疗多囊卵巢综合症的作用。展开更多
目的探讨苍附导痰丸治疗多囊卵巢综合征(Polycystic ovary syndrome,PCOS)的潜在机制。方法通过检索中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)中苍附...目的探讨苍附导痰丸治疗多囊卵巢综合征(Polycystic ovary syndrome,PCOS)的潜在机制。方法通过检索中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)中苍附导痰丸组成药物的成分数据,筛选其有效成分和作用靶点;检索Drug Bank、Gene Cards、OMIM、Pharm GKB及Uniprot疾病数据库中PCOS相关靶点;检索GEO数据库,选择多囊卵巢综合征的差异表达基因。构建苍附导痰丸和PCOS成分、靶点间的相互关系网络,利用筛选出的关键靶点,构建蛋白互作(Protein-protein interaction,PPI)网络;在GO功能和KEGG信号通路富集的基础上,得出苍附导痰丸治疗PCOS的作用机制,进一步用分子对接技术验证靶点之间结合的可靠性。结果共筛选出活性成分单体46个,159个苍附导痰丸治疗PCOS的共同靶点。结论苍附导痰丸治疗PCOS具有多成分、多靶点、多通路的特点,推测苍附导痰丸可能通过调节炎症微环境从而改善肥胖型PCOS患者胰岛素抵抗,通过调节雌激素信号通路改善卵巢功能,因而可以改善肥胖及卵巢排卵的临床症状,为后期研究苍附导痰丸治疗PCOS的作用机制提供了新的理论支撑。展开更多
基金Shanghai“Xinglin New Star”project[ZY(2018-2020)-RCPY-3012]Clinical study on improving the endometrial receptivity of patients with polycystic ovary syndrome by the method of invigorating the kidney,removing phlegm and promoting blood circulation(Project within budget of Shanghai university of traditional Chinese medicine)+1 种基金The construction project of Shanghai famous old traditional Chinese medicine experience research studio(No.SHGZS-2017026)Shanghai university of traditional Chinese medicine affiliated Shuguang hospital Siming fund(In-hospital project)
文摘Objective:To explore the target and molecular mechanism of Cangfu Daotan pill in the treatment of polycystic ovary syndrome(PCOS)by network pharmacology.Methods:The main active components and corresponding targets of Cangfu Daotan pills were searched in the database of traditional Chinese medicine systems pharmacology database and analysis platform(TCMSP).GeneCards database and OMIM database were used to search for disease targets of PCOS;the intersection of the target of Cangfu Daotan pill and the disease target of PCOS was drawn by using R to obtain the intersection gene;protein interaction network was constructed through String online database;Cytoscape(3.2.1)was used to construct a‘major component-disease-target’network diagram and analyze the important nodes therein;KEGG and GO enrichment analyses were carried out on the obtained intersection genes through org data packets in R.Results:Network pharmacology analysis showed that 151 active ingredients involved in pathways were screened out,140 of which acted on PCOS,and 105 potential targets were used to exert therapeutic effects.After enrichment,1900 GO biological signals and 143 KEGG signals were obtained,and the first 14 drug targets were screened out.Conclusion:Cangfu Daotan pill may mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation and other signals through regulating key targets of JUN,MAPK3,HSP90AA1,MAPK1,ESR1,NR3C1,APP,MAPK14,MAPK8,VEGFA,EGFR,RB1,AR,IL6,mediate gene transcription regulation,lipid metabolism,inflammatory micro environment,insulin resistance,sex hormone axis,arteriosclerosis,interleukin immune regulation,etc.,and regulate biological processes such as drug reaction,nutrition and lipid metabolism,active oxygen,sugar metabolism,steroid hormone,etc.to achieve the effect of treating polycystic ovary syndrome.
文摘目的:运用网络药理学方法探讨苍附导痰丸治疗多囊卵巢综合症(polycystic ovary syndrome,PCOS)的作用靶点及分子机制。方法:在中药系统药理学数据库(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索苍附导痰丸的主要活性成分及对应的作用靶点;利用GeneCards数据库和OMIM数据库搜索多囊卵巢综合症的疾病靶点;运用R数据包图绘制苍附导痰丸作用靶点和多囊卵巢综合症的疾病靶点的交集,得到交集基因;通过String在线数据库构建蛋白互作网络;利用Cytoscape(3.2.1)构建“主要成分-疾病-靶点”网络图,分析其中的重要节点;通过R语言下的org数据包对得到的交集基因进行KEGG和GO富集分析。结果:网络药理学分析表明,共筛选出151个有效活性成分涉及通路,其中作用于PCOS的140个,通过105个潜在靶点发挥疗效。富集得到1900条GO生物信号,143条KEGG信号,筛选出前14个药物靶点。结论:苍附导痰丸可能通过调控JUN、MAPK3、HSP90AA1、MAPK1、ESR1、NR3C1、APP、MAPK14、MAPK8、VEGFA、EGFR、RB1、AR、IL6关键靶点,介导基因转录调节、脂质代谢、炎性微环境、胰岛素抵抗、性激素轴、动脉硬化、白介素免疫调节等信号,调控药物反应、营养与脂质代谢、活性氧、糖代谢、类固醇激素等生物进程调控达到治疗多囊卵巢综合症的作用。
文摘目的探讨苍附导痰丸治疗多囊卵巢综合征(Polycystic ovary syndrome,PCOS)的潜在机制。方法通过检索中药系统药理学数据库和分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)中苍附导痰丸组成药物的成分数据,筛选其有效成分和作用靶点;检索Drug Bank、Gene Cards、OMIM、Pharm GKB及Uniprot疾病数据库中PCOS相关靶点;检索GEO数据库,选择多囊卵巢综合征的差异表达基因。构建苍附导痰丸和PCOS成分、靶点间的相互关系网络,利用筛选出的关键靶点,构建蛋白互作(Protein-protein interaction,PPI)网络;在GO功能和KEGG信号通路富集的基础上,得出苍附导痰丸治疗PCOS的作用机制,进一步用分子对接技术验证靶点之间结合的可靠性。结果共筛选出活性成分单体46个,159个苍附导痰丸治疗PCOS的共同靶点。结论苍附导痰丸治疗PCOS具有多成分、多靶点、多通路的特点,推测苍附导痰丸可能通过调节炎症微环境从而改善肥胖型PCOS患者胰岛素抵抗,通过调节雌激素信号通路改善卵巢功能,因而可以改善肥胖及卵巢排卵的临床症状,为后期研究苍附导痰丸治疗PCOS的作用机制提供了新的理论支撑。
