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Rapid Separation and Quantification of Iron in Uranium Nuclear Matrix by Capillary Zone Electrophoresis (CZE)
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作者 Vivekchandra Mishra Mrinal Kanti Das +2 位作者 Subbiah Jeyakumar Ramesh Mahadeo Sawant Karanam Lakshminarayana Ramakumar 《American Journal of Analytical Chemistry》 2011年第1期46-55,共10页
A method was developed for rapid separation and determination of iron by employing capillary zone elec-trophoresis (CZE) technique with direct UV detection. Iron could be separated from matrix uranium by di-rect injec... A method was developed for rapid separation and determination of iron by employing capillary zone elec-trophoresis (CZE) technique with direct UV detection. Iron could be separated from matrix uranium by di-rect injection of dissolved sample solution into capillary using a mixture of 10 mM HCl and 65 mM KCl (pH = 2) as background electrolyte (BGE) at an applied voltage of 15 kV. The developed method has a very high tolerance for the matrix element U (100 mg/mL) and as such may not need prior separation of iron from the matrix. Iron could be separated with better than 95% recovery. The method showed a linear calibration over a concentration range 1-50 ppm of Fe. The migration times for the iron peak were reproducible within 1% for both pure Fe(III) and in presence of matrix uranium (80 mg/mL). The precision (RSD, n = 22) of peak area obtained for 1ppm of iron was 3.5%. The limit of detection (LOD) (3 ) was 0.1 ppm and the ab-solute LOD was 9 × 10-14 g considering the sample injection volume of 1.5 nL. The developed method has been validated by separating and determining iron in two certified reference materials of U3O8. The method was applied for the determination of iron in different uranium based nuclear materials. The CZE method is versatile for routine analysis as it is simple, rapid and has simple sample preparation procedure. 展开更多
关键词 capillary zone electrophoresis IRON URANIUM Nuclear Industry Specification Analysis
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Interactions Between Thrombin and Natural Proudcts of Coreopsis tinctoria Nuttt. and Cistanche deserticola Ma. in Capillary Zone Electrophoresis 被引量:1
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作者 刘一 张媛 +3 位作者 刘晓明 凌笑梅 屠鹏飞 崔景荣 《Journal of Chinese Pharmaceutical Sciences》 CAS 2006年第1期38-44,共7页
Aim A capillary zone electrophoretic method (CZE) was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubat... Aim A capillary zone electrophoretic method (CZE) was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubated at 25 ℃ and then were separated by CZE with Tris-acetate buffer at pH 7.2. Each run could be accomplished within 5 min. Results In CZE, the peak width broadened due to the affinity interaction between natural products and thrombin. Compared with positive and negative control, the natural products (CB-1, CB-2) from Coreopsis tinctoria Nuttt. interacted with thrombin; CB-3 from Coreopsis tinctoria Nuttt. and HC-1, HC-2, HC-3 from Cistanche deserticola Ma. did not bind to thrombin. Both qualification and quantification characterizations of the binding were determined. Conclusion The established method is capable of sensitive and fast determination of natural products and thrombin interactions, it can be employed as an alternative method. 展开更多
关键词 capillary zone electrophoresis Coreopsis tinctoria Nuttt. Cistanche deserticola Ma INTERACTIONS THROMBIN
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A Peptides Migration Model in capillary Zone Electrophoresis
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作者 梁恒 王正刚 +2 位作者 顾峻岭 傅若农 林炳承 《Journal of Beijing Institute of Technology》 EI CAS 1996年第1期35-39,共5页
A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass ... A peptides migration model based on the principle of mechanics is presented in capillary zone electrophoresis (CZE). It is shown that the migration that the (tr) is a function of electric (Q), relative molecular mass (Mr), conformation parameter (Rc) of peptides and electrophoretic condition parameter(A). The conformation parameter is introduced to characterize multifarious shapes owing to the complex conlormation and the various kinds of macromolecules, where Rc≥1/3. The parameters A and Rc can be obtained from experimental data. The times of migration of the nine standard peptides were measured in pH 2.5buffer on different electrophoretic conditions in CZE. The experimental results agreed well with the theoretical prediction. 展开更多
关键词 capillary zone electrophoresis migration model conformation parameter
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Separation of Nucleotides in Swine Tissues Using Capillary Zone Electrophoresis
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作者 顾峻岭 刘玉 +2 位作者 傅若农 鲁红军 张燕婉 《Journal of Beijing Institute of Technology》 EI CAS 1994年第2期155-161,共7页
Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the me... Capillary zone electrophoresis has been applied to the analysis of nucleotides. The effects of buffer concentration. pH and other operating conditions on the separation were investigated and optimized. By using the method, separation and identification of nuclotides in swine tissues were completed. 展开更多
关键词 SEPARATION nucleotides/capillary zone electrophoresis
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p-Hydrazinobenzenesulfonic Acid Derivatives of Carbohydrates andTheir Capillary Zone Electrophoresis 被引量:6
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作者 Wang, XY Chen, Y 《Chinese Chemical Letters》 SCIE CAS CSCD 2000年第12期1081-1084,共4页
p-Hydrazinobenzenesulfonic acid is explored as a novel ultraviolet labeling reagent for capillary electrophoresis (CE) of mono- and disaccharides. The labeling reaction takes less than 10 minutes and introduces both o... p-Hydrazinobenzenesulfonic acid is explored as a novel ultraviolet labeling reagent for capillary electrophoresis (CE) of mono- and disaccharides. The labeling reaction takes less than 10 minutes and introduces both of absorption and charge groups into the sugars. 展开更多
关键词 p-hydrazinobenzenesulfonic acid CARBOHYDRATE DERIVATIZATION capillary zone electrophoresis
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Separation of chiral drugs with sulfobutylether-β-cyclodextrin bycapillary zone electrophoresis 被引量:1
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作者 Jun Ying LIU Yi Yang DONG +4 位作者 Tian Song WANG Hu Wei LIU Al Jin HUANG yi Liang SUNI Zeng Pei SUN(Department of Chemistry. Peking University. Beijing 100871)(Natlonal Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第1期39-42,共4页
Sulfobutylether-β-cyclodextrin(SBE-β-CD)was used as a chiral selector tor separatingten chlral drugs with resolution 1.2 by capillary zone electrophoresls(CZE), The backgroundelectrolylc solution compris... Sulfobutylether-β-cyclodextrin(SBE-β-CD)was used as a chiral selector tor separatingten chlral drugs with resolution 1.2 by capillary zone electrophoresls(CZE), The backgroundelectrolylc solution comprised of 120 mmol/L Britton-Robinson buffer(BRB) containing1 ~10mmol/L SBE-β-CD with the pH value adjusted from 5.0-6.8. Five of the drugs were better resolvedthan those previously reported with neutral CDs. 展开更多
关键词 Chiral separation sulfobutylether-β-cyclodextrin capillary zone electrophoresis anlsodamlne bencynonate ECONAZOLE ESMOLOL glycopynonate lobeline METHOXAMINE PHENYLEPHRINE plnacidll TERAZOSIN
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Capillary Zone Electrophoresis Investigation of Interactions between Granulocyte-colony Stimulating Factor and Dextran Sulfate/Carrageenan Oligosaccharide 被引量:1
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作者 AiYeLIANG YuGuangDU +1 位作者 KeYiWANG BingChengLIN 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第5期647-650,共4页
The interactions between granulocyte-colony stimulating factor (G-CSF) and dextran sulfate / κ-carrageenan oligosaccharide were studied by capillary zone electrophoresis. Dextran sulfate could strongly interact with ... The interactions between granulocyte-colony stimulating factor (G-CSF) and dextran sulfate / κ-carrageenan oligosaccharide were studied by capillary zone electrophoresis. Dextran sulfate could strongly interact with G-CSF and the complex was detected. The binding constant and stoichiometry were determined to be 1.2×106 (mol/L)-1 and 3:1, respectively. However, the interaction between κ-carrageenan oligosaccharide and G-CSF was not found. 展开更多
关键词 capillary zone electrophoresis granulocyte-colony stimulating factor dextran sulfate κ-carrageenan oligosaccharide INTERACTION
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Ionic Liquid and HP-β-CD Modified Capillary Zone Electrophoresis to Separate Hyperoside, Luteolin and Chlorogenic Acid 被引量:1
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作者 Yue Ling WANG Zhong Bo HU Zhuo Bin YUAN 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第2期231-234,共4页
Ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate, 1E-3MI-TFB) and HP-β- CD as modifier was added to the buffer to separate hyperoside, luteolin and chlorogenic acid. Experiments explored the effect of co... Ionic liquid (1-ethyl-3-methylimidazolium tetrafluoroborate, 1E-3MI-TFB) and HP-β- CD as modifier was added to the buffer to separate hyperoside, luteolin and chlorogenic acid. Experiments explored the effect of concentration of 1E-3MI-TFB and HP-β-CD on separation. The results indicated that 1.0 mmol/L HP-β-CD and 1%0 (v/v) 1E-3MI-TFB added to the buffer simultaneously could achieve a good compromise of resolution and analysis time. Capillary experiments and UV spectra indicated that there was interaction between 1E-3MI-TFB and analytes. 展开更多
关键词 Ionic liquid 1-ethyl-3-methylimidazolium tetrafluorobomte capillary zone electrophoresis lute.olin HYPEROSIDE chlorogenic acid.
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Studies of Active Ingredients in Cough Syrup by Capillary Zone Electrophoresis with Amperometric Detection 被引量:1
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作者 ZHOUTian-shu WANGAi-fang +2 位作者 WUFang SHIGuo-yue FANGYu-zhi 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2003年第4期386-389,共4页
The present paper covers a simple, reliable and reproducible method, based on capillary zone electrophoresis(CZE) with amperometric detection(AD), for the separation and the determination of ephedrine hydrochloride, p... The present paper covers a simple, reliable and reproducible method, based on capillary zone electrophoresis(CZE) with amperometric detection(AD), for the separation and the determination of ephedrine hydrochloride, promethazine hydrochloride and codeine phosphate. Under the optimal conditions, the three analytes were base-line separated completely within 16 min. Good linear relationships between the peak heights and the concentrations of the three analytes were obtained with the correlation coefficients better than 0 9993. The method was directly applied to the determination of the active ingredients in pharmaceutical preparations and the assay results were satisfactory. 展开更多
关键词 capillary zone electrophoresis Amperometric detection Ephedrine hydrochloride Promethazine hydrochloride Codeine phosphate
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Detection and Separation of Inorganic Cations in Natural, Potable, and Wastewater Samples Using Capillary Zone Electrophoresis with Indirect UV Detection 被引量:1
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作者 Lara Varden Fadi Bou-Abdallah 《American Journal of Analytical Chemistry》 2017年第1期81-94,共14页
Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify sever... Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify several inorganic cations in natural, potable, and wastewater samples. A pH 4.35 background electrolyte system was employed and consisted of 15 mM imidazole, 8 mM malonic acid, 2 mM 18-crown-6 ether as complexing agents, 10% v/v methanol as an organic modifier with indirect absorbance reference at 214 nm. The CZE method involved electromigration injection at 5 kV for 5 s, a separation voltage of 20 kV at 25℃, and a detection wavelength of 280 nm. Six main cations (ammonium , potassium K+, calcium Ca2+, sodium Na+, magnesium Mg2+, and lead Pb2+) were tested, and all but lead, were detected in the water samples at concentrations between 0.03 and 755 ppm with a detection limit ranging between 0.023 and 0.084 ppm. The successful evaluation of the proposed methodology allowed us to reliably detect and separate six metal ions in different water samples without any pretreatment. All water samples were collected from Northern New York towns and the Raquette River water system, the third longest river in New York State and the largest watershed of the central and western Adirondacks. 展开更多
关键词 capillary zone electrophoresis Indirect UV DETECTION INORGANIC CATIONS Northern New YORK Raquette River Adirondack Watershed
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Study of Interaction between Red-tide Toxin, Domoic Acid and Double stranded DNA by Capillary Zone Electrophoresis
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作者 DaZhiLI XinYaHE +2 位作者 HuiWANG LiSUN BingChengLIN 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第9期1079-1082,共4页
The interactions between amnesic red-tide toxin, domoic acid (DA) and 14mer double-stranded DNA (dsDNA with three kinds of sequences) were studied by capillary zone electrophoresis (CZE). For the dsDNA with a sequenc... The interactions between amnesic red-tide toxin, domoic acid (DA) and 14mer double-stranded DNA (dsDNA with three kinds of sequences) were studied by capillary zone electrophoresis (CZE). For the dsDNA with a sequence of 5'-CCCCCTATACCCGC-3', the amount of free dsDNA decreases with the increase of added DA; and the signal of DA-dsDNA complex was observed. Meanwhile, the other two dsDNAs, 5'-(C)12GC-3' and 5'-(AT)7-3', the existence of DA could not lead to the change of dsDNA signal and indicated that there is no interaction between DA and these two dsDNAs. 展开更多
关键词 DA DSDNA INTERACTION capillary zone electrophoresis.
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Evaluation of The Interaction between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis
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作者 Xin Ya HE Da Zhi LI +1 位作者 Ai Ye LIANG Bing Cheng LIN 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第3期304-307,共4页
Capillary zone electrophoresis (CZE) was applied to study the interaction between netropsin and a 14mer double stranded DNA (dsDNA). The binding constant of this interaction calculated from Scatchard plot was (1.070.... Capillary zone electrophoresis (CZE) was applied to study the interaction between netropsin and a 14mer double stranded DNA (dsDNA). The binding constant of this interaction calculated from Scatchard plot was (1.070.10)×105 (mol/L)-1. The binding stoichiometry was 1:1. The use of polyacrylamide coated capillary showed better effect in the analysis of DNA than noncoated capillary. 展开更多
关键词 INTERACTION netropsin DNA capillary zone electrophoresis.
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A Novel Method for Quantification of Circulating DNA in Serum by Capillary Zone Electrophoresis
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作者 PengZHANG JiCunREN ZuoJunSHEN 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第9期1091-1094,共4页
In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digeste... In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digested by proteinase to release free DNA, and then CZE-LIF system was used for the quantification of total circulating DNA. This method was successfully used to quantify the circulating DNA levels in sera from healthy individuals and certain cancer patients. We found the significantly elevated circulating DNA levels in certain prostate cancer patients. Our results demonstrated that CZE-LIF system has good linearity, excellent sensitivity (0.5 ng/mL DNA), satisfactory reproducibility (RSDs in one day and between days were both less than 5%) and reliability, and is well suitable to the quantification of the circulating DNA in human serum or plasma. 展开更多
关键词 Circulating DNA capillary zone electrophoresis quantification serum.
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Separation and Determination of Bases by Capillary Zone Electrophoresis
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作者 ZHAO Tao , LIU Qi-ping, CHENG Jie-ke (Department of Chemistry, Wuhan University , Wuhan , 430072) 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 1997年第3期215-220,共6页
The present paper covers the separation and determination of cytosine , 9-N-di- hydroxypropyltheophylline , theobromine , adenine , guanine , uracil , theophylline , 1-hydroxyethyl-5-fluorouracil , xanthine , uric aci... The present paper covers the separation and determination of cytosine , 9-N-di- hydroxypropyltheophylline , theobromine , adenine , guanine , uracil , theophylline , 1-hydroxyethyl-5-fluorouracil , xanthine , uric acid , 6-mercaptopurine , 5-fluo- rouracil and 1 -propionyloxyuracil by capillary zone electrophoresis. In an uncoated silica capillary(70 cm ×75 μm i. d. effective length 63 cm) , thirteen bases could be separated within 1 1 min under the optimum conditions : 0. 01 mol/L(pH 10. 0) bo- rate buffer, 40℃ , 15 kV, 260 nm and 2 s load. Efficiencies up to 3. 1 ×105 theoreti- cal plates (4. 9 ×105 plates/m) were achieved. 展开更多
关键词 capillary zone electrophoresis BASE electrophoresis
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Determination of Size Distribution of Nano-particles by Capillary Zone Electrophoresis
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作者 YanXUE HaiYingYANG YongTanYANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第1期67-70,共4页
A new method was developed for the determination of the size distribution of nano-particles by capillary zone electrophoresis (CZE). Scattering effect of nanoparticles was studied . This method for the determination... A new method was developed for the determination of the size distribution of nano-particles by capillary zone electrophoresis (CZE). Scattering effect of nanoparticles was studied . This method for the determination of size distribution was statistical. 展开更多
关键词 capillary zone electrophoresis polystyrene latex particle size distribution.
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Studies on Immunoglobulin G in Human Sera from Guillain Barre Syndrome Patients (Ⅰ)——The Separation of Immunoglobulin G by Capillary Zone Electrophoresis
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作者 RU Qin-hua WANG Yi-ming LUO Guo-an 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 1999年第4期367-370,共4页
关键词 capillary zone electrophoresis Immunoglobulin G Guillain Barre syndrome
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Separation of Purine and Its Derivatives by Capillary Zone Electrophoresis
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作者 Zeng Bai-zhao, Zhao Fa-qiongCollege of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, China 《Wuhan University Journal of Natural Sciences》 CAS 2003年第02A期437-442,共6页
The separation of a group of 17 purine and its derivatives by capillary zone electrophoresis is presented. A systematic approach was used to study the effect of pH, buffer type, organic modifiers, applied potential, s... The separation of a group of 17 purine and its derivatives by capillary zone electrophoresis is presented. A systematic approach was used to study the effect of pH, buffer type, organic modifiers, applied potential, sodium dodecyl sulfate (SDS) and cyclodextrins on the separation of these purine derivatives. An ideal condition was found for their separation, which was 30 mmol/L sodium borate buffer (pH 9&#8211;9.5), 10%(V/V) methanol buffer modifier and 20 kV. Under this condition, the 17 purine derivatives were baseline separated and the linear correlation coefficient for adenine, uric acid and 2-thioxanthine was 0. 99 over two orders of magnitude. The variation of peak areas was less than 4. 6%(n=5) and that of migration times was in the range of 0%&#8211;3%, while the samples were injected hydrodynamically at a height of 15 cm and an injection time of 8&#8211;10 s. In addition, alcohol, 1-propanol, 1-butanol and acetonitrile were also effective additives in the separation. However, SDS and various &#946;-cyclodextrin (&#946;-CDs) were found to do no good to their separation. 展开更多
关键词 purine derivatives capillary zone electrophoresis THIOPURINES
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CAPILLARY ZONE ELECTROPHORESIS OF NONOSACCHARIDES
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作者 RuiFeng YAO Yi CHEN Li Wen CHANG Institute of Chemistry,Academia Sinica,Beijing 100080 《Chinese Chemical Letters》 SCIE CAS CSCD 1993年第4期343-346,共4页
A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- ject... A method for the analysis of monosaccharides derived from glycoproteins and glycolipids was described using the newly developed technique of capillary zone electrophoresis(CZE).It is found that,at ca.0.5-5 nL in- jection,CZE can offer a high resolution of most monoses from glycoprotein and glycolipids.A mixture of amino-and non-aminomonosaccharides,which is difficult to separate with chromatographic methods,was separated complet- ely by CZE in borate buffer after the monosaccharides were derivatized with 1-naphthylamine. 展开更多
关键词 cze CHEN capillary zone electrophoresis OF NONOSACCHARIDES
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Quick Single Run Capillary Zone Electrophoresis Determination of Active Ingredients and Preservatives in Pharmaceutical Products
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作者 Marina Franco Renata Jasionowska 《American Journal of Analytical Chemistry》 2013年第3期117-124,共8页
The paper deals with the development of a rapid and efficient Capillary Zone Electrophoresis (CZE) method for Quality Control analysis of pharmaceutical preparations containing antihistamines, decongestants, anticholi... The paper deals with the development of a rapid and efficient Capillary Zone Electrophoresis (CZE) method for Quality Control analysis of pharmaceutical preparations containing antihistamines, decongestants, anticholinergic remedies and preservatives. Active ingredients of interest are: ChlorPheniramine Maleate (CPM), DiPhenhydramine Hydrochloride (DPH), Ephedrine hydrochloride (E), Isopropamide Iodide (II), Pheniramine Maleate (PM), Lidocaine hydrochloride (L), Tetracaine hydrochloride (T), Clopamide Hydrochloride (CH), DiHydroErgochristine (DHE), PhenylEphrine hydrochloride (PE) and Acetaminophen (A). Preservatives studied are: MethylParaben (MeP), EthylParaben (EtP), PropylParaben (PrP), ButylParaben (BuP), p-HydroxyBenzoic Acid (p-HBA). All these analytes were separated in a single run using 60 mM tetraborate buffer solution (TBS) pH = 9.2 as a BackGround Electrolyte (BGE) by using an uncoated fused silica capillary of I.D. = 50 mm and applying a voltage of 25 kV in the first part of the electropheretic run (up to 5.8 min) and 30 kV for the remaining time. The hydrodynamic pressurization of the inlet vial was 20 psi at 7.2 min. up to the end of analysis. Total separation time was of 7.5 min. The method was then successfully validated and applied to the simultaneous determination of active ingredients and preservatives. Good repeatability, linearity, and sensitivity were demonstrated. Precision of migration time (tm) was RSD% < 0.53% and of corrected peak area (Ac) was RSD% < 6.15%. The linearity evaluation gave 0.9928 2 1.000. LOD and LOQ, accuracy (recovery) and ruggedness were evaluated for each analyte demonstrating the good reliability of the method. Analyses of some pharmaceutical real samples were performed. 展开更多
关键词 capillary zone electrophoresis Nitrogenous Compound PARABENS PHARMACEUTICAL Analysis Quality Control Validation
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Determination of Melamine in Quail Egg and Milk with Capillary Zone Electrophoresis
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作者 Yu Kong Chong Wei +8 位作者 Zilong Wang Zhanwu Hou Hua Li Jiang Yu Jiaqiang Yuan Yongxi Zhao Jiangang Long Yuhai Tang Meili Gao 《American Journal of Analytical Chemistry》 2014年第4期258-265,共8页
A capillary zone electrophoresis method was developed for determination of melamine in food samples, such as quail egg and milk products. The CE procedure was performed on fused silica capillary (41 cm × 75 μm I... A capillary zone electrophoresis method was developed for determination of melamine in food samples, such as quail egg and milk products. The CE procedure was performed on fused silica capillary (41 cm × 75 μm I.D.) at 17 kV using pH3.1 60mmol/L phosphate buffer as run buffer and detecting at 200 nm. The proposed method showed good linearity (0.5 - 10.0 μmol/L) and low LOD (0.5 μmol/L) with good reproducibility (RSD% was 2.4 and 3.2 for migration time and peak area respectively), which made it suitable for quantity control of the related food product. 展开更多
关键词 capillary zone electrophoresis MELAMINE FOOD Products
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