Gap junction blocking agents can inhibit spontaneous discharge frequency in cells. We established a rat model of posttraumatic epilepsy induced using ferric ions. Rats were intraperitoneally injected with carbenoxolon...Gap junction blocking agents can inhibit spontaneous discharge frequency in cells. We established a rat model of posttraumatic epilepsy induced using ferric ions. Rats were intraperitoneally injected with carbenoxolone, 20 mg/kg, prior to and 30 minutes after model establishment, once a day for 14 consecutive days. Immunohistochemistry showed glial cell proliferation around a cortical focus and significantly increased connexin expression in posttraumatic epilepsy. However, carbenoxolone pretreatment or treatment significantly reduced connexin expression in the cortex, inhibited glial fibdllary acidic protein expression and ameliorated seizure degree in rats. These findings indicate that large amounts of glial cell proliferation and abnormal gap junction generation play a role in posttraumatic epilepsy, and that carbenoxolone may prevent and treat this disease.展开更多
Pectin is a biopolymer that has numerous useful purposes in food and beverage industry, cosmetic products and pharmaceutical fields. One of the important properties of pectin is its bioadhesive properties. Native pect...Pectin is a biopolymer that has numerous useful purposes in food and beverage industry, cosmetic products and pharmaceutical fields. One of the important properties of pectin is its bioadhesive properties. Native pectin provides fair bioadhesive properties that can be improved by modification of pectin structure. The immobilization of thiol groups on polymer can significantly improve the bioadhesive properties due to in situ cross-linking between thiol groups of polymer and mucin [1].展开更多
多梳蛋白家族(polycomb group proteins,PcG)是通过修饰染色质对靶基因进行转录抑制的表观遗传调控复合物。研究发现CBX4能介导与疾病发生的相关信号通路,从而参与增殖、分化及预后等生物学过程。该文就CBX4与肿瘤发生的相关机制及潜在...多梳蛋白家族(polycomb group proteins,PcG)是通过修饰染色质对靶基因进行转录抑制的表观遗传调控复合物。研究发现CBX4能介导与疾病发生的相关信号通路,从而参与增殖、分化及预后等生物学过程。该文就CBX4与肿瘤发生的相关机制及潜在的临床应用价值等方面的研究作一综述。展开更多
CBX蛋白(Chromobox protein homolog)是多梳蛋白家族(Polycomb group proteins,Pc G)的重要成员,多梳蛋白家族是一种表观遗传调控复合物,以聚合转录抑制复合体PRCs的形式存在,可以通过修饰染色质对靶基因进行转录抑制,在调控细胞分化、...CBX蛋白(Chromobox protein homolog)是多梳蛋白家族(Polycomb group proteins,Pc G)的重要成员,多梳蛋白家族是一种表观遗传调控复合物,以聚合转录抑制复合体PRCs的形式存在,可以通过修饰染色质对靶基因进行转录抑制,在调控细胞分化、衰老、死亡和肿瘤发生、转移中发挥着重要作用。CBX蛋白家族是经典型PRC1的重要成员,包括5个成员,CBX2、CBX4、CBX6、CBX7和CBX8,越来越多的证据表明它在肿瘤发生、发展中扮演重要角色。本篇综述阐述了目前关于CBX家族成员在肿瘤中的研究进展。展开更多
基金supported by the Social Development Program of Nantong, No. S2009035
文摘Gap junction blocking agents can inhibit spontaneous discharge frequency in cells. We established a rat model of posttraumatic epilepsy induced using ferric ions. Rats were intraperitoneally injected with carbenoxolone, 20 mg/kg, prior to and 30 minutes after model establishment, once a day for 14 consecutive days. Immunohistochemistry showed glial cell proliferation around a cortical focus and significantly increased connexin expression in posttraumatic epilepsy. However, carbenoxolone pretreatment or treatment significantly reduced connexin expression in the cortex, inhibited glial fibdllary acidic protein expression and ameliorated seizure degree in rats. These findings indicate that large amounts of glial cell proliferation and abnormal gap junction generation play a role in posttraumatic epilepsy, and that carbenoxolone may prevent and treat this disease.
文摘Pectin is a biopolymer that has numerous useful purposes in food and beverage industry, cosmetic products and pharmaceutical fields. One of the important properties of pectin is its bioadhesive properties. Native pectin provides fair bioadhesive properties that can be improved by modification of pectin structure. The immobilization of thiol groups on polymer can significantly improve the bioadhesive properties due to in situ cross-linking between thiol groups of polymer and mucin [1].
文摘目的利用生物信息学技术分析CBX1在胃癌中的表达,探讨CBX1与胃瘤临床病理特征的相关性,阐明CBX1对胃瘤早期诊断和预后的影响。方法利用Oncomine数据库、GEPIA数据库分析CBX1在胃癌组织和正常胃组织中转录水平的表达差异;利用Western blot检测CBX1在正常胃黏膜细胞GES-1和胃癌细胞SGC7901中的蛋白表达差异;利用Human Protein Atlas网站分析CBX1在胃癌组织及正常胃组织中翻译水平的表达差异;利用UALCAN数据库及Kaplan Meier-Plotter在线分析工具,阐明CBX1高表达与胃癌患者病理参数及患者生存率之间的关系;利用GEPIA数据库寻找与CBX1有紧密作用关系的蛋白,利用STRING数据库,构建蛋白质互作(PPI)网络,Scytoscape软件对PPI可视化,并利用Metascape网站进行基因本体富集分析,Starbase网站进行京都基因与基因组百科全书通路富集分析。结果CBX1在胃瘤中高表达,且与胃瘤的肿瘤分级、分期相关;生存分析结果显示CBX1高表达与胃癌患者生存率密切相关;富集分析结果显示与CBX1相互作用的蛋白主要发挥组蛋白修饰、mRNA组装功能。结论CBX1在胃瘤中呈高表达,并与胃瘤多个病理性指标相关。
文摘CBX蛋白(Chromobox protein homolog)是多梳蛋白家族(Polycomb group proteins,Pc G)的重要成员,多梳蛋白家族是一种表观遗传调控复合物,以聚合转录抑制复合体PRCs的形式存在,可以通过修饰染色质对靶基因进行转录抑制,在调控细胞分化、衰老、死亡和肿瘤发生、转移中发挥着重要作用。CBX蛋白家族是经典型PRC1的重要成员,包括5个成员,CBX2、CBX4、CBX6、CBX7和CBX8,越来越多的证据表明它在肿瘤发生、发展中扮演重要角色。本篇综述阐述了目前关于CBX家族成员在肿瘤中的研究进展。
