期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
Cellulase Producing Bacteria from the Wood-Yards on Kallai River Bank 被引量:2
1
作者 Sasidharan Sreedevi Sreedharan Sajith Sailas Benjamin 《Advances in Microbiology》 2013年第4期326-332,共7页
This study evaluates the influence of growth parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC) concentration and agitation on cellulase production from three bacterial strains, viz., Achromobacter xyl... This study evaluates the influence of growth parameters such as pH, temperature, Carboxy Methyl Cellulose (CMC) concentration and agitation on cellulase production from three bacterial strains, viz., Achromobacter xylosoxidans BSS4, Bacillus sp. BSS3 and Pseudomonas sp. BSS2 isolated from the wood-yards on Kallai river bank in Kerala. Production of cellulase by these isolates was detected using basal salt medium (BSM) with 0.5% CMC as supplement, and CMCase activity was confirmed by iodine test. Dinitrosalicylic acid method was employed for assaying the cellulase production by measuring the amount of glucose liberated in μmol/mL/min. Maximum enzyme production from Pseudomonas sp. BSS2 was at pH 8, 37℃ with 1% CMC and 150 rpm, and cellulase production increased from initial 49.84 U/mL to 91.28 U/mL after optimization. The highest enzyme activity from Bacillus sp. BSS3 was at pH 9, 37℃ with 1% CMC, 150 rpm, and cellulase production increased from initial 26.05 U/mL to 104.68 U/mL after optimization. The maximum enzyme production from A. xylosoxidans BSS4 was at pH 7, 40℃ with 0.5% CMC and 150 rpm, and cellulase production increased from initial 55.28 U/mL to 68.37 U/mL after optimization. Thus among the three isolates, Bacillus sp. BSS3 showed maximum enzyme yield which can be explored for further scale up studies with an industrial perspective. 展开更多
关键词 cellulase carboxy methyl CELLULOSE Dinitrosalicylic Acid Optimization SUBMERGED FERMENTATION
下载PDF
Selection of a Thermophilic Alkalitolerant Actinomycete and Conditions for CMCase Production
2
作者 齐云 赵林 +1 位作者 廖银章 谭欣 《Transactions of Tianjin University》 EI CAS 2005年第3期204-208,共5页
Five thermophilic strains that can degrade cellulose were isolated from the compost of a waste management in Guangzhou, China. Since one of them degraded cellulose effectively, it was chosen as the study strain. Based... Five thermophilic strains that can degrade cellulose were isolated from the compost of a waste management in Guangzhou, China. Since one of them degraded cellulose effectively, it was chosen as the study strain. Based on its morphology, spores′ susceptibility to heat, cell wall composition and other characteristics, the organism was classified as Thermomonospora fusca. Conditions for production of carboxy methyl cellulase (CMCase) were examined. The optimal temperature and pH value for enzyme production were 50 ℃ and 10.5, respectively. Cellulosic materials and easily metabolisable carbohydrates served as carbon sources for the growth of the strain. Only cotton, avicel,carboxy methyl cellulose (CMC) acted as potent inducers for the production of cellulases by this strain. Despite excellent growth on easily metabolisable carbohydrates, only constitutive levels of cellulases were produced. The optimal carbon and nitrogen sources for CMCase production were cotton and soybean respectively. The high thermostability, wide pH stability, and cheap nitrogen source show well potential use for composting treatment and commercial detergents. 展开更多
关键词 ACTINOMYCETES Thermomonospora fusca carboxy methyl cellulase (cmcase)
下载PDF
里氏木酶利用预处理小麦秆生产纤维素酶的应用研究 被引量:2
3
作者 王欲晓 周琳琳 《广州化工》 CAS 2012年第4期29-33,共5页
为了降低里氏木霉生产纤维素酶的成本,研究了小麦秸秆经白腐菌和酸处理后主要成分如纤维素、木聚糖、木质素含量的变化,采用正交实验考察了里氏木霉菌发酵小麦秆生产纤维素酶的最佳条件,研究得到的最佳条件为:小麦秆∶麸皮=2∶3,培养温... 为了降低里氏木霉生产纤维素酶的成本,研究了小麦秸秆经白腐菌和酸处理后主要成分如纤维素、木聚糖、木质素含量的变化,采用正交实验考察了里氏木霉菌发酵小麦秆生产纤维素酶的最佳条件,研究得到的最佳条件为:小麦秆∶麸皮=2∶3,培养温度30℃,起始pH 5.0,发酵时间48 h。通过对正交实验条件的优化,发酵液滤纸酶活(FPA)为6.11 IU.mL-1,羧甲基纤维素酶活(CMCase)为29.11 IU.mL-1,纤维二糖酶活(CBA)为16.11 IU.mL-1。和原工艺相比FPA、CMCase和CBA分别提高了30.78%、26.82%和37.11%。 展开更多
关键词 纤维素酶生产 预处理小麦秆 里氏木霉 滤纸酶活 羧甲基纤维素酶活 纤维二糖酶活
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部